Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast.

abstract：:Rh(phen)2phi3+ (phen = 1,10-phenanthroline; phi = 9,10-phenanthrenequinone diimine) avidly binds to DNA via intercalation from the major groove and upon photoactivation produces strand scission with single base 5' asymmetry. Enantiomers of Rh(phen)2phi3+, which lack hydrogen-bonding substituents in ancillary positions...

journal_title：Biochemistry

authors： Campisi D,Morii T,Barton JK

更新日期：1994-04-12 00:00:00

abstract：:β-2-Microglobulin (β2m) forms amyloid fibrils in the joints of patients undergoing dialysis treatment as a result of kidney failure. One of the ways in which β2m can be induced to form amyloid fibrils in vitro is via incubation with stoichiometric amounts of Cu(II). To better understand the structural changes caused b...

journal_title：Biochemistry

authors： Borotto NB,Zhang Z,Dong J,Burant B,Vachet RW

更新日期：2017-02-28 00:00:00

abstract：:Bacterioferritins are members of a class of spherical shell-like iron storage proteins that catalyze the oxidation and hydrolysis of iron at specific sites inside the protein shell, resulting in formation of a mineral core of hydrated ferric oxide within the protein cavity. Electrode oximetry/pH stat was used to study...

journal_title：Biochemistry

authors： Yang X,Le Brun NE,Thomson AJ,Moore GR,Chasteen ND

更新日期：2000-04-25 00:00:00

abstract：:The nonspecific phospholipase C from Bacillus cereus is a zinc metalloenzyme that catalyzes the hydrolysis of phospholipids to yield diacylglycerol and a phosphate monoester. Glu-4 has been proposed as a potential candidate for the general base in the hydrolysis reaction and was shown to interact with the substrate he...

journal_title：Biochemistry

authors： Tan CA,Roberts MF

更新日期：1998-03-24 00:00:00

abstract：:A specific, high-affinity binding site for ammodytoxin C in synaptic membranes from bovine cerebral cortex was detected and partially characterized. Equilibrium binding analysis revealed a single population of [125I]ammodytoxin C acceptors with the following binding parameters: Kd = 6.0 nM and Bmax = 5.7 pmol/mg membr...

journal_title：Biochemistry

authors： Krizaj I,Dolly JO,Gubensek F

更新日期：1994-11-22 00:00:00

abstract：:Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(II) dependent agmatinase (SpeB). This is the first report of an iron-dependent agmatinase. We demonstrate that aerobically isolated recombinant enzyme contains two disulfide bonds and only a trace amount of any metal and...

journal_title：Biochemistry

authors： Miller D,Xu H,White RH

更新日期：2012-04-10 00:00:00

abstract：:GPIbalpha is an integral membrane protein of the GPIb-IX-V complex found on the platelet surface that interacts with the A1 domain of von Willebrand factor (vWF-A1). The interaction of GPIbalpha with vWF-A1 under conditions of high shear stress is the first step in platelet-driven thrombus formation. Phage display was...

journal_title：Biochemistry

authors： Benard SA,Smith TM,Cunningham K,Jacob J,DeSilva T,Lin L,Shaw GD,Kriz R,Kelleher KS

更新日期：2008-04-22 00:00:00

abstract：:Amyloid formation by immunoglobulin light chain (LC) proteins is associated with amyloid light chain (AL) amyloidosis. Destabilization of the native state of the variable domain of the LC (VL) is known to be one of the critical factors in promoting the formation of amyloid fibrils. However, determining the key residue...

journal_title：Biochemistry

authors： Kobayashi Y,Tsutsumi H,Abe T,Ikeda K,Tashiro Y,Unzai S,Kamikubo H,Kataoka M,Hiroaki H,Hamada D

更新日期：2014-08-12 00:00:00

abstract：:Modification of the bacterial cell wall heteropolymer peptidoglycan by addition of an acetyl group to the C-6 hydroxyl group of N-acetylmuramoyl residues is known to inhibit the activity of muramidases (lysozymes) of innate immune systems. The O-acetylation of peptidoglycan also precludes the action of intrinsic lytic...

journal_title：Biochemistry

authors： Weadge JT,Clarke AJ

更新日期：2006-01-24 00:00:00

abstract：:Crowding, which characterizes the interior of all living cells, has been shown to dramatically affect biochemical processes, leading to stabilization of compact morphologies, enhanced macromolecular associations, and altered reaction rates. Due to the crowding-mediated shift in binding equilibria toward association, c...

journal_title：Biochemistry

authors： Goobes R,Kahana N,Cohen O,Minsky A

更新日期：2003-03-04 00:00:00

abstract：:The stability and folding kinetics of wild-type and a mutant staphylococcal nuclease (SNase) at neutral pH are significantly perturbed by the presence of moderate to high concentrations of salts. Very substantial increases in stability toward thermal and urea denaturation were observed; for example, 0.4 M sodium sulfa...

journal_title：Biochemistry

authors： Nishimura C,Uversky VN,Fink AL

更新日期：2001-02-20 00:00:00

abstract：:Unoccupied estrogen receptor (ER) can be extracted from tissues by homogenization with a hypotonic buffer, whereas hormone-occupied ER becomes tightly bound to the nuclear pellet and must be extracted with high-salt-containing buffers. The molecular basis for estrogen-induced tight nuclear binding of ER remains an imp...

journal_title：Biochemistry

authors： Fritsch M,Aluker M,Murdoch FE

更新日期：1999-06-01 00:00:00

abstract：:Phosphate was reported to be an inhibitor of copper- and zinc-containing superoxide dismutase (SOD) [de Freitas, D.M., & Valentine, J.S. (1984) Biochemistry 23, 2079-2082]. Thus SOD activity, in 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (pH 7.4), was decreased by approximately 50% when the assay...

journal_title：Biochemistry

authors： Beyer WF Jr,Wang Y,Fridovich I

更新日期：1986-10-07 00:00:00

abstract：:In Campylobacter jejuni 2,4-diacetamido-2,4,6-trideoxy-alpha-d-glucopyranose, termed N,N'-diacetylbacillosamine (Bac2,4diNAc), is the first carbohydrate in the glycoprotein N-linked heptasaccharide. With uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) as a starting point, two enzymes of the general protein glycos...

journal_title：Biochemistry

authors： Olivier NB,Chen MM,Behr JR,Imperiali B

更新日期：2006-11-14 00:00:00

abstract：:Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cb...

journal_title：Biochemistry

authors： Fedosov SN,Fedosova NU,Berglund L,Moestrup SK,Nexø E,Petersen TE

更新日期：2005-03-08 00:00:00

abstract：:Adenosyltransferases (ATRs) catalyze the synthesis of the reactive cobalt-carbon bond found in coenzyme B(12) or 5'-deoxyadenosylcobalamin (AdoCbl), which serves as a cofactor for a number of isomerases. The reaction involves a reductive adenosylation of cob(II)alamin in which an electron delivered by a reductase redu...

journal_title：Biochemistry

authors： Padovani D,Banerjee R

更新日期：2009-06-16 00:00:00

abstract：:Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...

journal_title：Biochemistry

authors： Cifonelli JA,King JA

更新日期：1977-05-17 00:00:00

abstract：:Polyhydroxybutyrate (PHB) synthases catalyze the conversion of beta-hydroxybutyryl coenzyme A (HBCoA) to PHB. These enzymes require an active site cysteine nucleophile for covalent catalysis. A protein BLASTp search using the Class III Chromatium vinosum synthase sequence reveals high homology to prokaryotic lipases w...

journal_title：Biochemistry

authors： Jia Y,Kappock TJ,Frick T,Sinskey AJ,Stubbe J

更新日期：2000-04-11 00:00:00

abstract：:Identical first-order rate constants for phosphorylation of the calcium ATPase of sarcoplasmic reticulum by bound inorganic phosphate (Pi) of 25 +/- 2 s-1 with empty vesicles and 25 +/- 1 s-1 with vesicles that were passively loaded with 40 mM Ca2+ were obtained by treating the reaction as an approach to equilibrium (...

journal_title：Biochemistry

authors： Myung J,Jencks WP

更新日期：1995-03-07 00:00:00

abstract：:Complete 1H NMR sequential assignments have been made for copper(I)- and silver (I)-substituted metallothionein (MT) from Saccharomyces cerevisiae using standard 2D 1H NMR methods. The fingerprint region of the COSY spectrum of both metalloproteins shows a doubling of a few backbone proton resonances from residue K41 ...

journal_title：Biochemistry

authors： Narula SS,Winge DR,Armitage IM

更新日期：1993-07-06 00:00:00

abstract：:We have previously suggested that folding of the SV40 attenuator RNA 1 into two hairpin elements leads to a block of transcription elongation. Using site-directed mutagenesis, we created three templates that either strengthened or weakened the proposed hairpin structures. The mutated and wild-type templates were clone...

journal_title：Biochemistry

authors： Goldring NB,Kessler M,Aloni Y

更新日期：1992-09-08 00:00:00

abstract：:Highly purified tryptic fragments of calmodulin were tested for their ability to stimulate adenylate cyclase activity of Bordetella pertussis spheroplast membranes and were compared to their activities on brain Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase. The C-terminal fragment, consisting of residu...

journal_title：Biochemistry

authors： Wolff J,Newton DL,Klee CB

更新日期：1986-12-02 00:00:00

abstract：:DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...

journal_title：Biochemistry

authors： Berardini M,Mackay W,Loechler EL

更新日期：1997-03-25 00:00:00

abstract：:The complex of dioldehydrase with adenosylcobalamin (coenzyme B12) and potassium ion reacts with molecular oxygen in the absence of a substrate to oxidize coenzyme and inactivate the complex. In this article, high performance liquid chromatography and mass spectral analysis are used to identify the nucleoside products...

journal_title：Biochemistry

authors： Schwartz PA,Frey PA

更新日期：2007-06-19 00:00:00

abstract：:Thrombospondin-1 (TSP1) binding to calreticulin (CRT) on the cell surface signals focal adhesion disassembly, leading to the intermediate adhesive phenotype, cell migration, anoikis resistance, and collagen stimulation. Residues Lys 24 and 32 in TSP1 and amino acids 24-26 and 32-34 in CRT have been shown through bioch...

journal_title：Biochemistry

authors： Yan Q,Murphy-Ullrich JE,Song Y

更新日期：2011-02-01 00:00:00

abstract：:The properties of the Fe and Mo sites of the iron-molybdenum cofactor of nitrogenase with respect to binding and activation of N(2) have been studied by molecular mechanics calculations on the local protein environment and by density functional theory (DFT) calculations on subsections of the cofactor. The DFT calculat...

journal_title：Biochemistry

authors： Durrant MC

更新日期：2002-11-26 00:00:00

abstract：:In bee venom phospholipase A2, histidine-34 probably functions as a Brønsted base to deprotonate the attacking water. Aspartate-64 and tyrosine-87 form a hydrogen bonding network with histidine-34. We have prepared mutants at these positions and studied their kinetic properties. The mutant in which histidine-34 is cha...

journal_title：Biochemistry

authors： Annand RR,Kontoyianni M,Penzotti JE,Dudler T,Lybrand TP,Gelb MH

更新日期：1996-04-09 00:00:00

abstract：:We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesi...

journal_title：Biochemistry

authors： Sidik K,Smerdon MJ

更新日期：1990-08-14 00:00:00

abstract：:Circular dichroism (CD) spectra at variable temperatures have been recorded for human cytosolic sialidase NEU2 in buffered water solutions and in the presence of divalent cations. The results show the prevalence of β-strands together with a considerable amount of α-helical structure, while in the solid state, from bot...

journal_title：Biochemistry

authors： Monti E,Benaglia G,Mozzi A,Fusi P,Longhi G,Gangemi F,Castiglioni E,Woody RW,Fornili SL,Abbate S

更新日期：2014-08-19 00:00:00

abstract：:The fluorescent nucleotide analogue of ADP, 1,N6-ethenoadenosine diphosphate (epsilon ADP), has been used to probe the active site of myosin subfragment 1 (SF1). The Mg complex of ADP was shown to be trapped stoichiometrically at the active site by a variety of thiol cross-linking agents having sulfur to sulfur spanni...

journal_title：Biochemistry

authors： Perkins WJ,Wells JA,Yount RG

更新日期：1984-08-14 00:00:00