Abstract:
:Highly purified tryptic fragments of calmodulin were tested for their ability to stimulate adenylate cyclase activity of Bordetella pertussis spheroplast membranes and were compared to their activities on brain Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase. The C-terminal fragment, consisting of residues 78-148, was a full agonist for the cyclase with 0.1-0.15 the potency of calmodulin but did not stimulate phosphodiesterase. Fragments 1-77, 1-90, and 107-148 stimulated adenylate cyclase (and not phosphodiesterase) at low potency; this was not due to calmodulin contamination, but contamination by fragment 78-148 could not be excluded with certainty. An adduct of norchlorpromazine isothiocyanate and calmodulin showed full agonist activity for adenylate cyclase at 0.01-0.02 the potency of calmodulin. Stimulation of adenylate cyclase by a number of the fragments occurred in the absence of Ca2+, but stimulator potency was enhanced 20-60-fold in its presence. The similarity of Ca2+ requirements of fragment 78-148 and calmodulin suggests that occupancy of the two C-terminal Ca2+ binding sites of calmodulin accounts for most of the Ca2+ enhancement of calmodulin stimulation of adenylate cyclase.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wolff J,Newton DL,Klee CBdoi
10.1021/bi00372a025subject
Has Abstractpub_date
1986-12-02 00:00:00pages
7950-5issue
24eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Fluorescence lifetimes and fluorescence anisotropy decays of the voltage-sensitive styryl-pyridinium dye RH421 have been measured in the presence of dimyristoylphosphatidylcholine vesicles, the water soluble enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco), and Na+,K(+)-ATPase-containing membrane fragm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00037a015
更新日期:1995-09-19 00:00:00
abstract::Uncontrolled proteolysis due to cathepsin G (cat G) may cause severe pathological disorders. Cat G is inhibited by alpha 1-antichymotrypsin (ACT) and alpha 1-proteinase inhibitor (alpha 1PI), two members of the serpin superfamily of proteins. To see whether these two inhibitors play a physiological proteolysis-prevent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980223q
更新日期:1998-08-11 00:00:00
abstract::NADH:ubiquinone oxidoreductase (NDH-1 or complex I) from Escherichia coli was purified using a combination of anion exchange chromatography and centrifugation in sucrose density gradient. The dependence of enzyme activity on detergent and phospholipids was studied. Artificial hexaammineruthenium reductase activity was...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050134v
更新日期:2005-06-14 00:00:00
abstract::The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as leaving groups is described. It is demonstrated that th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952758e
更新日期:1996-06-04 00:00:00
abstract::The primary electron acceptor complex of photosystem II, QAFe2+, can bind a number of small molecules at the iron site, including cyanide [Koulougliotis, D., Kostopoulos, T., Petrouleas, V., & Diner, B. A. (1993) Biochim. Biophys. Acta 1141, 275-282)]. In the presence of NaCN (30-300 mM) at pH 6.5, the reduced state, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00199a014
更新日期:1994-08-23 00:00:00
abstract::At any instant, the human erythrocyte sugar transporter presents at least one sugar export site but multiple sugar import sites. The present study asks whether the transporter also presents more than one sugar exit site. We approached this question by analysis of binding of [3H]cytochalasin B (an export conformer liga...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015586w
更新日期:2001-12-25 00:00:00
abstract::The Escherichia coli repressor of biotin biosynthesis is both a biotin ligase and the repressor of transcriptional initiation at the biotin biosynthetic operon. The small molecule, biotinyl-5'-adenylate (bio-5'-AMP), is the intermediate in the biotin ligation reaction and the positive allosteric effector for sequence-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00189a041
更新日期:1994-06-14 00:00:00
abstract::The structure of HIV protease (HIV Pr) bound to JE-2147 (also named AG1776 or KNI-764) is determined here to 1.09 A resolution. This highest-resolution structure for HIV Pr allows refinement of anisotropic displacement parameters (ADPs) for all atoms. Clustering based on the directional information in ADPs defines two...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011781z
更新日期:2002-04-09 00:00:00
abstract::13C NMR has been used to observe the equilibrium complex of [5,5-2H,5-13C]-5-aminolevulinate [( 5,5-2H,5-13C]ALA) bound to porphobilinogen (PBG) synthase (5-aminolevulinate dehydratase), a 280,000-dalton protein. [5,5-2H,5-13C]ALA (chemical shift 46.9 ppm in D2O) was prepared from [5-13C]ALA through enolization in deu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00412a039
更新日期:1988-06-14 00:00:00
abstract::Trifluoperazine (TFP) was shown to interact with the cyanogen bromide fragment 9 (CB9) (residues 84-135) of rabbit skeletal troponin C and with a synthetic peptide representing the N-terminal region of CB9. The phenothiazine did not affect the calcium binding property of CB9 as observed by proton magnetic resonance an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00276a010
更新日期:1983-03-29 00:00:00
abstract::The molecular mass (M(r)) of the nickel- and iron-sulfur-containing enzyme CO dehydrogenase from Clostridium thermoaceticum was determined by sedimentation equilibrium ultracentrifugation to be 300,000 +/- 30,000 Da. Since the enzyme is known to contain equal numbers of two types of subunits (M(r) = 82,000 Da for alph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9511853
更新日期:1996-02-13 00:00:00
abstract::In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035150b
更新日期:2003-12-30 00:00:00
abstract::Charybdotoxin (ChTX) and iberiotoxin (IbTX) are highly charged peptidyl toxins which exhibit 68% sequence identity and share a similar three-dimensional structure. Despite these structural similarities, IbTX and ChTX differ in their selectivity for two types of potassium channels; large conductance calcium-activated p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00060a030
更新日期:1993-03-09 00:00:00
abstract::IFABP is a small beta-barrel protein with a short helix-turn-helix motif near the N-terminus that is thought to participate in the regulation of the uptake and delivery of fatty acids. In a previous work, we detected by near UV circular dichroism a reversible conformational transition of this protein occurring between...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020680d
更新日期:2003-06-24 00:00:00
abstract::Hepatic microsomal cytochrome P-450 from phenobarbital-pretreated rats is destroyed by propyne in a reduced nicotinamide adenine dinucleotide dependent process which also results in vivo in the accumulation of an abnormal green porphyrin. The green porphyrin has been identified by its electronic absorption, mass spect...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00528a033
更新日期:1981-12-08 00:00:00
abstract::The kinetics of the slow folding and unfolding reactions of barstar, a bacterial ribonuclease inhibitor protein, have been studied at 23(+/-1) degrees C, pH 8, by the use of tryptophan fluorescence, far-UV circular dichroism (CD), near-UV CD, and transient mixing (1)H nuclear magnetic resonance (NMR) spectroscopic mea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990285w
更新日期:1999-07-13 00:00:00
abstract::Oxidative DNA damage can lead to cancer, and as enzymatic DNA repair systems become compromised during the aging process, the role of exogenous antioxidants becomes more critical. Here, we examined whether such non-enzymatic DNA repair can be effected by the common cellular antioxidant glutathione, investigating both ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00697
更新日期:2019-11-19 00:00:00
abstract::The thermodynamic quantities of the anti-dinitrophenyl antibody-hapten interaction are reported for rabbit, goat, and guinea pig antibodies. Rabbit and goat antibodies had similar exothermic enthalpy changes for their reaction with 2,4-dinitrophenyl-L-lysine (-13.9 and -14.8 kcal/mol, respectively). The enthalpy chang...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00694a032
更新日期:1975-11-18 00:00:00
abstract::In order to elucidate the molecular mechanisms responsible for the apparent nonlinear behavior of the temperature dependence of the redox potential of Hydrogenobacter thermophilus cytochrome c552 [Takahashi, Y., Sasaki, H., Takayama, S. J., Mikami, S., Kawano, S., Mita, H., Sambongi, Y., and Yamamoto, Y. (2006) Bioche...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7000714
更新日期:2007-08-14 00:00:00
abstract::The carbon-13 (13C) nuclear magnetic resonance (NMR) behavior of the hyperactive analogue of luteinizing-hormone-releasing hormone (LH-RH), des-Gly-NH2(10)-[D-Leu6]LH-RH ethylamide, has been studied. The spectra are compared with those of the hypoactive des-Gly-NH2(10)-[L-Leu6]LH-RH ethylamide. Spin-lattice relaxation...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00666a020
更新日期:1976-10-19 00:00:00
abstract::Serine hydroxymethyltransferase (SHMT) catalyzes the reversible conversion of l-serine and tetrahydrofolate into glycine and 5,10-methylenetetrahydrofolate. This enzyme, which plays a pivotal role in one-carbon metabolism, is involved in cancer metabolic reprogramming and is a recognized target of chemotherapy interve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01074
更新日期:2018-12-26 00:00:00
abstract::14C-Labeled fluorodinitrobenzene and N-ethylmaleimide have been used as chemical probes of the conformational states of myosin induced by the binding of MgADP and MgATP. The results indicate that in the high-energy conformation, MMgADP-Pi, the essential thiols are protected from modification but their diminished react...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00643a005
更新日期:1977-11-29 00:00:00
abstract::Unfolded apomyoglobin in 8 M urea at pH 2.3 displays distinct regions with different backbone mobility, as monitored by NMR relaxation. These variations in backbone mobility can be correlated with intrinsic properties of the amino acids in the sequence. Clusters of small amino acids such as glycine and alanine show in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020381o
更新日期:2002-10-22 00:00:00
abstract::The light-sensing outer segments of photoreceptor cells harbor hundreds of flattened membranous discs containing the visual pigment, rhodopsin, and all the proteins necessary for visual signal transduction. PRCD (progressive rod-cone degeneration) protein is one of a few proteins residing specifically in photoreceptor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00489
更新日期:2016-09-13 00:00:00
abstract::A T203Y substitution in green fluorescent protein causes a red shift in emission to yield a class of mutants known as yellow fluorescent protein (YFP). Many of these YFP mutants bind halides with affinities in the millimolar range, which often results in the chromophore pK values being shifted into the physiological r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3016839
更新日期:2013-04-09 00:00:00
abstract::The Escherichia coli ATP-consuming chaperonin machinery, a complex between GroEL and GroES, has evolved to facilitate folding of substrate proteins (SPs) that cannot do so spontaneously. A series of kinetic experiments show that the SPs are encapsulated in the GroEL/ES nanocage for a short duration. If confinement of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00903
更新日期:2021-01-19 00:00:00
abstract::The homologous series of optically active short-chain phosphatidylethanolamines (PE) from dibutyryl-PE to dioctanoyl-PE was synthesized. In addition, two monomeric short-chain phospholipid analogues that are not degraded by phospholipase A2 (1,2-bis[(butylcarbamyl)oxy]-sn-glycero-3-phosphocholine and the corresponding...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00336a058
更新日期:1985-07-16 00:00:00
abstract::The structure of octylcarbamoyl-alpha-chymotrypsin to a resolution of 3.0 A is described. The n-octyl side chain of the active site directed irreversible inactivator octyl isocyanate is bound exclusively in the hydrophobic substrate binding pocket. The n-octyl isocyanate forms a planar urethane bond with the Ser-195 O...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00694a008
更新日期:1975-11-18 00:00:00
abstract::Changes in the concentrations of high-energy phosphate metabolites were measured by 31P NMR spectroscopy of surviving rat uteri from 0-48 h following estrogen administration. Concentrations (millimoles per kilogram wet weight) of these metabolites in the untreated immature uterus, measured at 4 degrees C, were found t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00307a006
更新日期:1984-06-05 00:00:00
abstract::Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation,...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.5b01073
更新日期:2016-03-22 00:00:00