当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Activation of isolated NADH:ubiquinone reductase I (complex I) from Escherichia coli by detergent and phospholipids. Recovery of ubiquinone reductase activity and changes in EPR signals of iron-sulfur clusters.

Activation of isolated NADH:ubiquinone reductase I (complex I) from Escherichia coli by detergent and phospholipids. Recovery of ubiquinone reductase activity and changes in EPR signals of iron-sulfur clusters.

Abstract：

:NADH:ubiquinone oxidoreductase (NDH-1 or complex I) from Escherichia coli was purified using a combination of anion exchange chromatography and centrifugation in sucrose density gradient. The dependence of enzyme activity on detergent and phospholipids was studied. Artificial hexaammineruthenium reductase activity was not affected by dodecyl maltoside (DDM) and asolectin. Ubiquinone reductase activity had a bell-shape dependence on DDM concentration; 7-10-fold activation could be achieved. Treatment with asolectin subsequently yields additional 2-fold activation with a corresponding increase in the apparent V(max) and without significant changes in apparent K(m). Comparative EPR studies of complex I reduced with NADH, "as prepared" and "activated by asolectin" showed an increase in the signals derived mainly from two [4Fe-4S] clusters in the activated enzyme. One of these signals could be simulated with an axial spectrum with g values of g(xyz)= 1.895, 1.904, 2.05, which corresponds to the parameters reported for the N2 cluster. This data indicates conformational rearrangements of catalytic importance in complex I upon binding of phospholipids.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Sinegina L,Wikström M,Verkhovsky MI,Verkhovskaya ML

doi

10.1021/bi050134v

subject

Has Abstract

pub_date

2005-06-14 00:00:00

pages

8500-6

issue

23

eissn

0006-2960

issn

1520-4995

journal_volume

44

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Structure, dynamics, and antimicrobial and immune modulatory activities of human LL-23 and its single-residue variants mutated on the basis of homologous primate cathelicidins.

    abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2016266

    authors： Wang G,Elliott M,Cogen AL,Ezell EL,Gallo RL,Hancock RE

    更新日期：2012-01-17 00:00:00

  • Stimulation of the maltose transporter ATPase by unliganded maltose binding protein.

    abstract：:ATP hydrolysis by the maltose transporter (MalFGK(2)) is regulated by maltose binding protein (MBP). Binding of maltose to MBP brings about a conformational change from open to closed that leads to a strong stimulation of the MalFGK(2) ATPase. In this study, we address the long-standing but enigmatic observation that ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9007066

    authors： Gould AD,Telmer PG,Shilton BH

    更新日期：2009-08-25 00:00:00

  • The impact of the E46K mutation on the properties of alpha-synuclein in its monomeric and oligomeric states.

    abstract：:The third and most recently identified Parkinson's disease-linked variant of the neuronal protein alpha-synuclein to be identified (E46K) results in widespread brain pathology and early onset Parkinson symptoms (Zarranz et al. (2004) Ann. Neurol. 55, 164-173). Herein, we present biochemical and biophysical characteriz...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7000246

    authors： Fredenburg RA,Rospigliosi C,Meray RK,Kessler JC,Lashuel HA,Eliezer D,Lansbury PT Jr

    更新日期：2007-06-19 00:00:00

  • Natural mutagenesis study of the human steroid 5 alpha-reductase 2 isozyme.

    abstract：:The enzyme steroid 5 alpha-reductase utilizes NADPH to reduce the double bonds of a variety of steroid substrates with generalized 3-oxo, delta 4,5 structures. One substrate for this membrane-bound enzyme is testosterone, whose reduction to dihydrotestosterone is required for the embryonic differentiation of the exter...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00171a029

    authors： Wigley WC,Prihoda JS,Mowszowicz I,Mendonca BB,New MI,Wilson JD,Russell DW

    更新日期：1994-02-08 00:00:00

  • Kinetics of ribosome dissociation and subunit association studied in a light-scattering stopped-flow apparatus.

    abstract：:The association-dissociation kinetics of ribosomes from Escherichia coli have been studied under various conditions in a light-scattering stopped-flow apparatus. The dissociation reaction at 2 mg/ml at 25 degrees C, induced by lowering the MgCl2 concentration from 18 to 3 mM, can best be described by three independent...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00671a010

    authors： Görisch H,Goss DJ,Parkhurst LJ

    更新日期：1976-12-28 00:00:00

  • Stacking of bacteriochlorophyll c macrocycles in chlorosome from Chlorobium limicola as revealed by intermolecular 13C magnetic-dipole correlation, X-ray diffraction, and quadrupole coupling in 25Mg NMR.

    abstract：:The stacking of the bacteriochlorophyll (BChl) c macrocycles and the role of water in forming an aggregate sheet, in chlorosome, were examined by means of (13)C NMR spectroscopy, the measurement of the X-ray diffraction pattern, and (25)Mg NMR spectroscopy. (1) The stacking of the macrocycles, i.e., weakly overlapped ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801651w

    authors： Kakitani Y,Koyama Y,Shimoikeda Y,Nakai T,Utsumi H,Shimizu T,Nagae H

    更新日期：2009-01-13 00:00:00

  • Three-dimensional structures of the amyloid beta peptide (25-35) in membrane-mimicking environment.

    abstract：:The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 expe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961598j

    authors： Kohno T,Kobayashi K,Maeda T,Sato K,Takashima A

    更新日期：1996-12-17 00:00:00

  • Characterization of the basic amphiphilic alpha-helix calmodulin-binding domain of a 61.5 kDa tobacco calmodulin-binding protein.

    abstract：:A 19-amino acid residue peptide, Gly-Trp-Leu-Lys-Ile-Lys-Ala-Ala-Met-Arg-Trp-Gly-Phe-Phe-Val-Arg-Lys-Lys- Ala, corresponding to the basic amphiphilic alpha-helix (BAA) motif at the C-terminus of a recombinant tobacco calmodulin-binding protein, TCB60, was synthesized. The interaction of the synthetic binding domain wi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9621674

    authors： Dash S,Niemaczura W,Harrington HM

    更新日期：1997-02-25 00:00:00

  • Mechanism of yeast cytochrome b2 action. I. Thermodynamics and relaxation kinetics of the interaction between cytochrome b2 and oxalate.

    abstract：:Oxalate is the strongest known inhibitor of yeast cytochrome b2 activity. We have used spectrophotometric titration, temperature-jump relaxation, and calorimetry in an investigation of the interaction between enzyme and inhibitor. The titration data are consistent with noncooperative binding to one site per subunit. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00647a002

    authors： Thusius D,Blazy B,Baudras A

    更新日期：1976-01-27 00:00:00

  • Light-induced changes in the chemical bond structure of light-harvesting complex II probed by FTIR spectroscopy.

    abstract：:Light-harvesting complex II (LHC-II) regulates the light energy distribution between photosystem I and II in plants. This process is mediated by phosphorylation of the LHC-II protein, which depends on the oxidation state of photosynthetic electron carriers. In addition to this regulatory mechanism, it has recently bee...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034114+

    authors： Rogl H,Kühlbrandt W,Barth A

    更新日期：2003-09-02 00:00:00

  • Energetics of peptide recognition by the second PDZ domain of human protein tyrosine phosphatase 1E.

    abstract：:Formation of protein-protein assemblies is essential in maintaining cell structure and function. Conservation of structural motifs and binding sites is the result of evolutionary pressure for solutions compatible with both molecular economy and regulation. PDZ domains are a typical example: A conserved fold governs sp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061869i

    authors： Milev S,Bjelić S,Georgiev O,Jelesarov I

    更新日期：2007-01-30 00:00:00

  • Phenothiazine-binding and attachment sites of CAPP1-calmodulin.

    abstract：:In the presence of Ca2+ norchlorpromazine isothiocyanate forms a monocovalent complex with calmodulin: CAPP1-calmodulin (Newton et al, 1983). Trypsin digestion of [3H]CAPP1-calmodulin yields as the major radioactive peptide N epsilon-CAPP-Lys-Met-Lys, corresponding to residues 75-77 of calmodulin. Stoichiometric amoun...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00435a019

    authors： Newton DL,Klee CB

    更新日期：1989-05-02 00:00:00

  • Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and the esterification of cholesterol in human long term lymphoid cell lines.

    abstract：:The regulation of the rate-controlling enzyme in cholesterol biosynthesis and of the incorporation of [14C]oleate into cholesterol esters were studied in established lymphoid cell lines from normal subjects and compared with that of eight patients with genetic abnormalities of lipid metabolism. The activity of 3-hydro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00648a011

    authors： Kayden HJ,Hatam L,Beratis NG

    更新日期：1976-02-10 00:00:00

  • The affinity of elongation factor Tu for an aminoacyl-tRNA is modulated by the esterified amino acid.

    abstract：:When different mutations were introduced into the anticodon loop and at position 73 of YFA2, a derivative of yeast tRNA(Phe), a single tRNA body was misacylated with 13 different amino acids. The affinities of these misacylated tRNAs for Thermus thermophilus elongation factor Tu (EF-Tu).GTP were determined using a rib...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi036290o

    authors： Dale T,Sanderson LE,Uhlenbeck OC

    更新日期：2004-05-25 00:00:00

  • Effects of metal ligation and oxygen on the reversibility of the thermal denaturation of Pseudomonas aeruginosa azurin.

    abstract：:Thermodynamic equilibrium transition models in DSC are only applicable to reversible processes, but reversibility of the thermal transitions of proteins is comparatively rare because of intermolecular aggregation of denatured proteins and the degradation that occurs at high temperatures. The cupredoxin azurin from Pse...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0157621

    authors： Sandberg A,Leckner J,Shi Y,Schwarz FP,Karlsson BG

    更新日期：2002-01-22 00:00:00

  • Effect of alpha-methylation on inactivation of monoamine oxidase by N-cyclopropylbenzylamine.

    abstract：:Monoamine oxidase (MAO) was shown previously [Silverman, R. B., & Hoffman, S. J. (1980) J. Am. Chem. Soc. 102, 7126-7128] to catalyze the oxidation of N-cyclopropylbenzylamine (N-CBA) at two sites on the molecule. Oxidation at the benzyl methylene gave benzaldehyde and cyclopropylamine; oxidation of the cyclopropyl gr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00317a019

    authors： Silverman RB

    更新日期：1984-10-23 00:00:00

  • Ligand-induced variations in the reactivity of thio groups of the alpha-subunit of the acetylcholine receptor from Torpedo californica.

    abstract：:We have studied alkylation of the acetylcholine receptor by N-[3H]ethylmaleimide ([3H]NEM) under various conditions. The radiolabeled preparations were submitted to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to separate the receptor complex into subunits, and the incorporation of 3H into each type of ch...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00306a001

    authors： de Souza Otero A,Hamilton SL

    更新日期：1984-05-22 00:00:00

  • Protonmotive activity of cytochrome c oxidase: control of oxidoreduction of the heme centers by the protonmotive force in the reconstituted beef heart enzyme.

    abstract：:This paper contributes to the characterization of partial steps of electron and proton transfer in mitochondrial cytochrome c oxidase with respect to their membrane arrangement and involvement in energy-linked protonmotive activity. It is shown that delta psi controls electron flow from cytochrome c to heme a is consi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00464a008

    authors： Capitanio N,De Nitto E,Villani G,Capitanio G,Papa S

    更新日期：1990-03-27 00:00:00

  • Regulation of plasminogen activation by components of the extracellular matrix.

    abstract：:The kinetics of activation of Glu-plasminogen (Glu-Pg) and Lys77-Pg by two-chain recombinant tissue plasminogen activator (t-PA) were determined in the presence of isolated protein components of the extracellular matrix (ECM) and compared to activation in the presence of fibrinogen and fibrinogen fragments and in the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00472a029

    authors： Stack S,Gonzalez-Gronow M,Pizzo SV

    更新日期：1990-05-22 00:00:00

  • Manganese (II) and substrate interaction with unadenylylated glutamine synthetase (Escherichia coli w). II. Electron paramagnetic resonance and nuclear magnetic resonance studies of enzyme-bound manganese(II) with substrates and a potential transition-sta

    abstract：:The enhancement of the longitudinal proton relaxation rate of solvent water protons which occurs when Mn(II) is bound to the "tight" metal ion site of unadenylylated glutamine synthetase (GS) was used to determine the binding constant of L-methionine (SR)-sulfoximine to GS-Mn(II) complexes. The binary enhancement for ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00648a014

    authors： Villafranca JJ,Ash DE,Wedler FC

    更新日期：1976-02-10 00:00:00

  • High-resolution structure of an HIV zinc fingerlike domain via a new NMR-based distance geometry approach.

    abstract：:A new method is described for determining molecular structures from NMR data. The approach utilizes 2D NOESY back-calculations to generate simulated spectra for structures obtained from distance geometry (DG) computations. Comparison of experimental and back-calculated spectra, including analysis of cross-peak buildup...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00454a005

    authors： Summers MF,South TL,Kim B,Hare DR

    更新日期：1990-01-16 00:00:00

  • Altering the reaction specificity of eukaryotic ornithine decarboxylase.

    abstract：:Ornithine decarboxylase (ODC) catalyzes the first committed step in the biosynthesis of polyamines, and it has been identified as a drug target for the treatment of African sleeping sickness, caused by Trypanosoma brucei. ODC is a pyridoxal 5'-phosphate (PLP) dependent enzyme and an obligate homodimer. X-ray structura...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001209s

    authors： Jackson LK,Brooks HB,Osterman AL,Goldsmith EJ,Phillips MA

    更新日期：2000-09-19 00:00:00

  • Role of mitogen-activated protein kinases in S-nitrosoglutathione-induced macrophage apoptosis.

    abstract：:The inflammatory mediator nitric oxide (NO*) promotes apoptotic cell death based on morphological evidence, accumulation of the tumor suppressor p53, caspase-3 activation, and DNA fragmentation in RAW 264.7 macrophages. Since nitrosothiols may actually be the predominant form of biologically active NO* in vivo, we use...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982292a

    authors： Callsen D,Brüne B

    更新日期：1999-02-23 00:00:00

  • Cholesterol dynamics in membranes of raft composition: a molecular point of view from 2H and 31P solid-state NMR.

    abstract：:Lipidic membrane systems that have been reported to be composed of sphingomyelin (SM)-cholesterol (Chol) microdomains or "rafts" by Dietrich et al. [palmitoyloleoyl-phosphatidylcholine(POPC)/SM/Chol, 1/1/1; Dietrich, C., Bagatolli, L. A., Volovyk, Z. N., Thompson, N. L., Levi, M., Jacobson, K., and Gratton, E. (2001) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026717b

    authors： Aussenac F,Tavares M,Dufourc EJ

    更新日期：2003-02-18 00:00:00

  • The orientation and accessibility of substrates on the active site of triosephosphate isomerase.

    abstract：:Tritiated sodium borohydride was used to reduce the substrates of triosephosphate isomerase in the presence of the enzyme, and the mixture of the four possible products (D-[1(R)-3H]; D-[1(S)-3H]-; D-[2-3H]-, and L-[2-3H]glycerol 3-phosphate) was analyzed. While enzyme-bound dihydroxyacetone phosphate is reduced comple...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00692a020

    authors： Webb MR,Knowles JR

    更新日期：1975-10-21 00:00:00

  • Identification of the protein 4.1 binding site to phosphatidylserine vesicles.

    abstract：:Previous studies have shown that protein 4.1 is a multifunctional protein that binds to spectrin, actin, glycophorins, the anion channel protein, and phosphatidylserine (PS). In this report, we have characterized the binding of protein 4.1 and its major proteolytic fragments to phospholipid vesicles. Pure 125I-labeled...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00402a018

    authors： Cohen AM,Liu SC,Lawler J,Derick L,Palek J

    更新日期：1988-01-26 00:00:00

  • High resolution solution structure of the 1.3S subunit of transcarboxylase from Propionibacterium shermanii.

    abstract：:Transcarboxylase (TC) from Propionibacterium shermanii, a biotin-dependent enzyme, catalyzes the transfer of a carboxyl group from methylmalonyl-CoA to pyruvate to form propionyl-CoA and oxalacetate. Within the multi-subunit enzyme complex, the 1.3S subunit functions as the carboxyl group carrier and also binds the ot...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9925367

    authors： Reddy DV,Shenoy BC,Carey PR,Sönnichsen FD

    更新日期：2000-03-14 00:00:00

  • Benzothiazepine binding domain of purified L-type calcium channels: direct labeling using a novel fluorescent diltiazem analogue.

    abstract：:We have synthesized a series of N-propylamino-substituted benzazepinones (NPSBs) as specific probes for the benzothiazepinone (BTZ) binding domain of muscle L-type calcium channels (LTCCs). NPSBs were identified which possess high affinity for the channel after purification. We synthesized a fluorescent NPSB, DMBODIPY...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00010a039

    authors： Brauns T,Cai ZW,Kimball SD,Kang KC,Haugland RP,Berger W,Berjukov S,Hering S,Glossmann H,Striessnig J

    更新日期：1995-03-14 00:00:00

  • Equilibrium and kinetics of the reaction of Aplysia myoglobin with azide.

    abstract：:The present paper reports a study on the equilibria and kinetics of the acid-alkaline transition and the azide binding reaction by ferric Aplysia myoglobin. A single completely reversible spectrophotometric titration curve is found over the pH range from similar to 5 to similar to 9, with an apparent pK equals to 7.5 ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00679a006

    authors： Giacometti GM,Da Ros A,Antonini E,Brunori M

    更新日期：1975-04-22 00:00:00

  • Analysis of the reaction of carbachol with acetylcholinesterase using thioflavin T as a coupled fluorescence reporter.

    abstract：:Acetylcholinesterase (AChE) contains a narrow and deep active site gorge with two sites of ligand binding, an acylation site (or A-site) at the base of the gorge and a peripheral site (or P-site) near the gorge entrance. The P-site contributes to catalytic efficiency by transiently binding substrates on their way to t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8015197

    authors： Rosenberry TL,Sonoda LK,Dekat SE,Cusack B,Johnson JL

    更新日期：2008-12-09 00:00:00