Abstract：
:In the presence of Ca2+ norchlorpromazine isothiocyanate forms a monocovalent complex with calmodulin: CAPP1-calmodulin (Newton et al, 1983). Trypsin digestion of [3H]CAPP1-calmodulin yields as the major radioactive peptide N epsilon-CAPP-Lys-Met-Lys, corresponding to residues 75-77 of calmodulin. Stoichiometric amounts of all other expected tryptic peptides are also found, indicating that norchlorpromazine isothiocyanate selectively acylates Lys 75. A second molecule of CAPP-NCS can react, albeit slowly, with calmodulin to form CAPP2-calmodulin. Fragments 38-74 and 127-148 are completely missing from the trypsin digests of CAPP2-calmodulin without deliberate exposure to UV irradiation. Possibly the lengthy preparation of CAPP2-calmodulin favors photolysis, caused by room lights, of the putative CAPP-binding domains located in these two peptides. Lys 148, the sole lysyl residue in fragment 127-148, is a probable site of attachment of the second molecule of CAPP. UV irradiation of CAPP1-calmodulin, followed by digestion with trypsin, results in the selective loss of 50% each of peptides containing residues 38-74 and 127-148, suggesting that these peptides contain the hydrophobic amino acids that form the phenothiazine-binding sites. The loss of peptides encompassing residues 38-74 and 127-148, located in the amino and carboxyl halves of calmodulin, respectively, suggests that the hydrophobic rings of CAPP can bind at either one of the two phenothiazine sites. Computer modeling of CAPP1-calmodulin with the X-ray coordinates of calmodulin (Babu et al., 1986) indicates that CAPP attached to Lys 75 cannot interact with the carboxyl-terminal phenothiazine-binding site.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Newton DL,Klee CBdoi
10.1021/bi00435a019subject
Has Abstractpub_date
1989-05-02 00:00:00pages
3750-7issue
9eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The bacterial second messenger signaling molecule bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) controls important biological processes such as biofilm formation, virulence response, and motility. This second messenger is sensed by macromolecular targets inside the cell, both protein and RNA, which ind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301510v
更新日期：2013-01-15 00:00:00
abstract：:We investigated the dependence of certain properties of bile salt/lecithin mixed micelles on the molar ratio of the two components. UV absorption suggested that the micellar system is fundamentally altered on passing through a bile salt:lecithin molar ratio of 1.8:1. Differential scanning calorimetry confirmed this tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00505a029
更新日期：1981-01-20 00:00:00
abstract：:The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydroxy acid substrates t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01199
更新日期：2017-01-17 00:00:00
abstract：:The Ndi1 enzyme found in the mitochondrial membrane of Saccharomyces cerevisiae is an NDH-2-type alternative NADH-quinone oxidoreductase. As Ndi1 is expected to be a possible remedy for complex I defects of mammalian mitochondria, a detailed biochemical characterization of the enzyme is needed. To identify the ubiquin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100005j
更新日期：2010-04-06 00:00:00
abstract：:Quantum tunneling and protein dynamics have emerged as important components of enzyme function. This review focuses on soybean lipoxygenase-1, to illustrate how the properties of enzymatic C-H bond activation link protein motions to the fundamental bond making-breaking processes. ...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi301504m
更新日期：2013-03-26 00:00:00
abstract：:Recent work has resulted in the development of potent inhibitors of oligosaccharyl transferase (OT), the enzyme that catalyzes the cotranslational glycosylation of asparagine [Hendrickson, T. L., Spencer, J. R., Kato, M., and Imperiali, B. (1996) J. Am. Chem. Soc. 118, 7636-7637; Kellenberger, C., Hendrickson, T. L., ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982740e
更新日期：1999-04-27 00:00:00
abstract：:The virally encoded integrase protein carries out retroviral integration, which requires specific interactions with the two ends of the viral DNA, and also with host DNA that is the target of integration. We attached a photo-cross-linking agent to specific viral and target DNA sites to identify regions of the integras...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972949c
更新日期：1998-05-12 00:00:00
abstract：:5-HT(2A) serotonin receptors represent the principal molecular targets for LSD-like hallucinogens and atypical antipsychotic drugs. It has been proposed that a dysregulation of 5-HT(2A) receptor-mediated signaling may contribute to the pathogenesis of schizophrenia and related diseases. A major mechanism for the atten...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035061z
更新日期：2003-09-16 00:00:00
abstract：:Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed tha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00439a003
更新日期：1989-06-27 00:00:00
abstract：:Chicken histone H4, labeled separately at Met-84 with N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid and 5-(iodoacetamido)fluorescein, was reassociated with unlabeled histones H2A, H2B, and H3 and 146 base pairs of DNA to produce fluorescently labeled nucleosomes having physical characteristics virtually...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00366a010
更新日期：1986-09-09 00:00:00
abstract：:Mutagenesis of Escherichia coli manganese superoxide dismutase (MnSD) demonstrates involvement of the strictly conserved gateway tyrosine (Y34) in exogenous ligand interactions. Conservative replacement of this residue by phenylalanine (Y34F) affects the pH sensitivity of the active-site metal ion and perturbs ligand ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9704212
更新日期：1997-07-22 00:00:00
abstract：:The quercetin binding sites on spinach chloroplast coupling factor 1 (CF1) have been investigated using direct and competitive binding, stopped-flow, temperature-jump, and fluorescence resonance energy transfer measurements. It was found that 8-anilino-1-naphthalensulfonic acid (ANS) competes with quercetin binding at...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00646a001
更新日期：1976-01-13 00:00:00
abstract：:In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970160w
更新日期：1997-09-23 00:00:00
abstract：:In order to define structural regions in thrombin that interact with hirudin, the N alpha-dinitrofluorobenzyl analogue of an undecapeptide was synthesized corresponding to residues 54-64 of hirudin [GDFEEIPEEY(O35SO3)L (DNFB-[35S]Hir54-64)]. DNFB-[35S]Hir54-64 was reacted at a 10-fold molar excess with human alpha-thr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00479a006
更新日期：1990-07-10 00:00:00
abstract：:The objective of this work is to determine the molecular mechanism and regulation of short-chain acyl-CoA dehydrogenase (SCAD) from Megasphaera elsdenii. To achieve this, the gene coding for SCAD from M. elsdenii was cloned and sequenced. Site-directed mutagenesis was then used to identify an amino acid residue that i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00091a026
更新日期：1993-10-12 00:00:00
abstract：:Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00439a004
更新日期：1989-06-27 00:00:00
abstract：:Transport of lactate, pyruvate, and the ketone bodies, acetoacetate and beta-hydroxybutyrate, is mediated in many mammalian cells by the monocarboxylate transporter MCT1. To be accepted as a substrate, a carboxyl group and an unpolar side chain are necessary. Site-directed mutagenesis of the rat MCT1 was used to ident...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990973f
更新日期：1999-08-31 00:00:00
abstract：:The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00207a004
更新日期：1994-10-18 00:00:00
abstract：:We introduce here a new model to describe the binding of extrinsic membrane proteins to acidic lipid membranes. In this view, macroscopic binding affinity is determined by two processes: nonspecific adsorption of protein to the membrane surface and association of acidic lipids with specific sites on the bound protein....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00444a045
更新日期：1989-09-05 00:00:00
abstract：:Alditol oxidase (AldO) from Streptomyces coelicolor A3(2) is a soluble monomeric flavin-dependent oxidase that performs selective oxidation of the terminal primary hydroxyl group of several alditols. Here, we report the crystal structure of the recombinant enzyme in its native state and in complex with both six-carbon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701886t
更新日期：2008-01-22 00:00:00
abstract：:Fluorescence-detected circular dichroism (FDCD) spectra are reported for ethidium in Escherichia coli cells and bound to E. coli DNA in vitro. FDCD bands are observed at 325 and 385 nm. These bands change amplitude as the ethidium to DNA ratio changes. Spectra are similar for in vivo and in vitro measurements. However...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00333a001
更新日期：1985-06-04 00:00:00
abstract：:The biosynthesis of the antitumor antibiotic sibiromycin by Streptosporangium sibiricum requires the construction of four units: the amino sugar from glucose; the anthranilate ring from DL-tryptophan probably via kynurenine; the aromatic methyl group from methionine; the propylidene proline from L-tyrosine with the lo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00586a029
更新日期：1979-09-18 00:00:00
abstract：:Isoforms of transforming growth factor beta (TGFbeta) are 25 kDa homodimeric polypeptides that signal by binding and bringing together two related, functionally distinct cell surface receptors designated as TbetaR1 and TbetaR2. Here, we report the solution structure of the 13.8 kDa extracellular domain of human TbetaR...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034366a
更新日期：2003-09-02 00:00:00
abstract：:Protein farnesyltransferase catalyzes the posttranslational farnesylation of several proteins involved in signal transduction, including Ras, and is a target enzyme for antitumor therapies. Efficient product formation catalyzed by protein farnesyltransferase requires an enzyme-bound zinc cation and high concentrations...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0011781
更新日期：2000-10-10 00:00:00
abstract：:Modification of the bacterial cell wall heteropolymer peptidoglycan by addition of an acetyl group to the C-6 hydroxyl group of N-acetylmuramoyl residues is known to inhibit the activity of muramidases (lysozymes) of innate immune systems. The O-acetylation of peptidoglycan also precludes the action of intrinsic lytic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051679s
更新日期：2006-01-24 00:00:00
abstract：:Photoactivated adenylyl cyclases are powerful tools for optogenetics and for investigating signal transduction mechanisms in biological photoreceptors. Because of its large increase in enzyme activity in the light, the BLUF (blue light sensor using flavin adenine dinucleotide)-activated adenylyl cyclase (bPAC) from Be...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500479v
更新日期：2014-08-12 00:00:00
abstract：:The fluorescence characteristics of brevin and the effects of Ca2+ on the protein conformation were fully investigated. Brevin contains 18 tryptophans and 27 tyrosines. Analysis of the fluorescence spectra and the accessibility to quenching molecules indicate that the emitting tryptophans are located in a hydrophobic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00341a055
更新日期：1985-09-24 00:00:00
abstract：:Enolase is a glycolytic metalloenzyme involved in carbon metabolism. The advantage of targeting enolase lies in its essentiality in many biological processes such as cell wall formation and RNA turnover and as a plasminogen receptor. We initially used a DARTS assay to identify enolase as a target in Escherichia coli. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01298
更新日期：2019-03-05 00:00:00
abstract：:The importance of two buried salt bridges in barnase in the stability of its folded state, the major transition rate for unfolding, and a folding intermediate has been analyzed by protein engineering, kinetic, and thermodynamic studies. The aspartate residues in the bridges Arg69-Asp93 and Arg83-Asp75 were replaced by...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952930e
更新日期：1996-05-28 00:00:00
abstract：:Resonance energy-transfer approaches have been used to directly monitor the interactions of the GTP gamma S-bound alpha subunit of transducin (alpha T GTP gamma S) with the retinal cyclic GMP phosphodiesterase (PDE). The PDE was labeled with 5-(iodoacetamido) fluorescein (IAF-PDE) and served as the fluorescence donor ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00243a011
更新日期：1991-07-23 00:00:00
