Key residues for the light regulation of the blue light-activated adenylyl cyclase from Beggiatoa sp.

abstract：:Treatment of soybean lipoxygenase isozyme 1 with its substrates, linoleic acid and oxygen, or product, 13(S)-hydroperoxy-9,11(Z,E)-octadecadienoic acid (13-HPOD), leads to the appearance of a purple color. Although the structure of the chromophore has not been determined, we present strong evidence that it is an Fe(3+...

journal_title：Biochemistry

authors： Nelson MJ,Chase DB,Seitz SP

更新日期：1995-05-09 00:00:00

abstract：:Fully functional variable fragments (Fv) of D1.3, a mouse antibody directed against the hen egg lysozyme, were readily produced as hybrids (Fv-MalE) with the maltose-binding protein of Escherichia coli and purified independently of their antigen-binding properties. We used site-directed mutations of residues in the co...

journal_title：Biochemistry

authors： England P,Brégégère F,Bedouelle H

更新日期：1997-01-07 00:00:00

abstract：:Slow conformational changes have been proposed to be responsible for the kinetic positive cooperativity of glucokinase (GK) with glucose. Induced-fit and preexisting equilibrium kinetic models have been previously suggested. In the present study, equilibrium and pre-steady-state fluorescence spectroscopy has been used...

journal_title：Biochemistry

authors： Antoine M,Boutin JA,Ferry G

更新日期：2009-06-16 00:00:00

abstract：:The flash-induced Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon S1-to-S2 transition (S2/S1 spectrum) was measured using photosystem II (PS II) core complexes of Synechocystis 6803 in which tyrosine residues were specifically labeled with 13C at the ring-4 position. The do...

journal_title：Biochemistry

authors： Noguchi T,Inoue Y,Tang XS

更新日期：1997-12-02 00:00:00

abstract：:Human transferrin (Tf) is responsible for the binding and transport of iron in the bloodstream of vertebrates. Delivery of this bound iron to cells occurs by a process of receptor-mediated endocytosis during which Tf releases its iron at the reduced endosomal pH of approximately 5.6. Iron release from Tf involves a la...

journal_title：Biochemistry

authors： Nurizzo D,Baker HM,He QY,MacGillivray RT,Mason AB,Woodworth RC,Baker EN

更新日期：2001-02-13 00:00:00

abstract：:We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiff...

journal_title：Biochemistry

authors： Shrivastava R,Rai A,Salapaka M,Sivaramakrishnan S

更新日期：2019-11-26 00:00:00

abstract：:In Bacillus subtilis, P protein is the noncatalytic component of ribonuclease P (RNase P) that is critical for achieving maximal nuclease activity under physiological conditions. P protein is predominantly unfolded (D) at neutral pH and low ionic strength; however, it folds upon the addition of sulfate anions (ligands...

journal_title：Biochemistry

authors： Henkels CH,Oas TG

更新日期：2005-10-04 00:00:00

abstract：:G-Quadruplexes occupy important regulatory regions in the genome. DNA G-quadruplexes in the promoter regions and RNA quadruplexes in the UTRs (untranslated regions) have been individually studied and variously implicated at different regulatory levels of gene expression. However, the formation of G-quadruplexes in the...

journal_title：Biochemistry

authors： Agarwal T,Roy S,Kumar S,Chakraborty TK,Maiti S

更新日期：2014-06-17 00:00:00

abstract：:We recently introduced a new substrate rescue tool for investigating enzymatic base flipping by uracil DNA glycosylase (UDG) in which a bulky pyrene nucleotide wedge (Y) was placed opposite a uracil in duplex DNA (i.e., a U/Y pair), thereby preorganizing the target base in an extrahelical conformation [Jiang, Y. L., e...

journal_title：Biochemistry

authors： Jiang YL,Stivers JT,Song F

更新日期：2002-09-17 00:00:00

abstract：:Several different monoclonal antibodies (mAbs) have been actively developed in the field of Alzheimer's disease (AD) for basic science and clinical applications; however, the binding kinetics of many of the mAbs with the beta-amyloid peptides (Abeta) are poorly understood. A panel of mAbs with different Abeta recognit...

journal_title：Biochemistry

authors： Ramakrishnan M,Kandimalla KK,Wengenack TM,Howell KG,Poduslo JF

更新日期：2009-11-03 00:00:00

abstract：:The binding of the R17 coat protein to synthetic RNAs containing one or two coat protein binding sites was characterized by using nitrocellulose filter and gel-retention assays. RNAs with two available sites bound coat protein in a cooperative manner, resulting in a higher affinity and reduced sensitivity to pH, ionic...

journal_title：Biochemistry

authors： Witherell GW,Wu HN,Uhlenbeck OC

更新日期：1990-12-18 00:00:00

abstract：:Green sulfur bacteria possess two light-harvesting antenna systems, the chlorosome and the Fenna-Matthews-Olson (FMO) protein. In addition to self-aggregated bacteriochlorophyll (BChl) c, chlorosomes of Chlorobium tepidum contain a small amount of BChl a (ratio 100:1). The chlorosomal BChl a is associated with CsmA, a...

journal_title：Biochemistry

authors： Pedersen MØ,Pham L,Steensgaard DB,Miller M

更新日期：2008-02-05 00:00:00

abstract：:Amyloid-beta (Abeta) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Abeta amyloid fibril using magnetically aligned preparations of a central Abeta domain which forms representative amyloid fibrils. Diffraction ...

journal_title：Biochemistry

authors： Serpell LC,Blake CC,Fraser PE

更新日期：2000-10-31 00:00:00

abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...

journal_title：Biochemistry

authors： Cho YK,Rebholz KL,Northrop DB

更新日期：1994-08-16 00:00:00

abstract：:The triplet lifetime and absorption anisotropy decay of eosin-labeled band 3 was measured in resealed erythrocyte ghosts. Membrane potentials were generated by the addition of valinomycin in the presence of a K+ gradient. Neither negative nor positive membrane potentials had any detectable effect on the rotational dif...

journal_title：Biochemistry

authors： Wyatt K,Cherry RJ

更新日期：1992-05-19 00:00:00

abstract：:Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemi...

journal_title：Biochemistry

authors： Wichmann JK,Schnoes HK,DeLuca HF

更新日期：1981-12-22 00:00:00

abstract：:Carbonic anhydrase (CA) catalyzes the reversible hydration of carbon dioxide to hydrogen carbonate, and its role in maintaining pH balance has made it an attractive drug target. Steroidal sulfamate esters, inhibitors of the cancer drug target steroid sulfatase (STS), are sequestered in vivo by CA II in red blood cells...

journal_title：Biochemistry

authors： Cozier GE,Leese MP,Lloyd MD,Baker MD,Thiyagarajan N,Acharya KR,Potter BV

更新日期：2010-04-27 00:00:00

abstract：:Pantothenate synthetase (PS, EC 6.3.2.1) is the last enzyme in the pantothenate biosynthesis pathway, a metabolic pathway identified as a potential target for new antimicrobials. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Here we report the overexpression, purificati...

journal_title：Biochemistry

authors： Satoh A,Konishi S,Tamura H,Stickland HG,Whitney HM,Smith AG,Matsumura H,Inoue T

更新日期：2010-08-03 00:00:00

abstract：:The photocycle of 124 kDa phytochrome A from Avena sativa was studied by Fourier-transform infrared spectroscopy at low temperatures. Difference spectra between the parent state Pr and the intermediates of the Pr-->Pfr pathway, i.e. lumi-R, meta-Ra, and meta-Rc, and between Pfr and the intermediates of the Pfr-->Pr pa...

journal_title：Biochemistry

authors： Foerstendorf H,Mummert E,Schäfer E,Scheer H,Siebert F

更新日期：1996-08-20 00:00:00

abstract：:Mycobacterium smegmatis MshC catalyzes the ATP-dependent condensation of GlcN-Ins and l-cysteine to form l-Cys-GlcN-Ins, the penultimate step in mycothiol biosynthesis. Attempts to crystallize the native, full-length MshC have been unsuccessful. However, incubation of the enzyme with the cysteinyl adenylate analogue, ...

journal_title：Biochemistry

authors： Tremblay LW,Fan F,Vetting MW,Blanchard JS

更新日期：2008-12-16 00:00:00

abstract：:The behavior of fluorescent and spin-label probes is examined in several fluid and gel phospholipid phases, with particular focus on the Ca2+-induced gel phase in phosphatidylserine (PS). These probes have behavior characteristic of the type of probe and of the type of lipid environment. Anthroyloxy- and doxyl-labeled...

journal_title：Biochemistry

authors： Florine KI,Feigenson GW

更新日期：1987-03-24 00:00:00

abstract：:Calf brain tubulin was subjected to isoelectric focusing and tryptic peptide map analysis. Results from isoelectric focusing experiments showed a total number of 17 well-resolved protein peaks. The number of peaks and the mass distribution under each peak remained the same when the concentration of protein or ampholyt...

journal_title：Biochemistry

authors： George HJ,Misra L,Field DJ,Lee JC

更新日期：1981-04-28 00:00:00

abstract：:Oxalate is the strongest known inhibitor of yeast cytochrome b2 activity. We have used spectrophotometric titration, temperature-jump relaxation, and calorimetry in an investigation of the interaction between enzyme and inhibitor. The titration data are consistent with noncooperative binding to one site per subunit. T...

journal_title：Biochemistry

authors： Thusius D,Blazy B,Baudras A

更新日期：1976-01-27 00:00:00

abstract：:Recent crystallographic data suggest that conserved residues in the connection subdomain and C-terminal ribonuclease H (RNase H) domain of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) contact the nascent DNA primer and modulate the trajectory of the template relative to the RNase H catalytic ce...

journal_title：Biochemistry

authors： Rausch JW,Lener D,Miller JT,Julias JG,Hughes SH,Le Grice SF

更新日期：2002-04-16 00:00:00

abstract：:Tandem calponin-homology (CH) domains are the most common actin-binding domains in proteins. However, structural principles underlying their function are poorly understood. These tandem domains exist in multiple conformations with varying degrees of inter-CH-domain interactions. Dystrophin and utrophin tandem CH domai...

journal_title：Biochemistry

authors： Bandi S,Singh SM,Mallela KM

更新日期：2015-09-08 00:00:00

abstract：:Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) is a key element in a signal transduction pathway that couples expression of genes required for glycerol metabolism to the relative availability of glycerol and glucose. Its catalytic activity is inhibited by protein-protein interactions...

journal_title：Biochemistry

authors： Liu WZ,Faber R,Feese M,Remington SJ,Pettigrew DW

更新日期：1994-08-23 00:00:00

abstract：:Anthrax toxin receptor 1 (ANTXR1)/tumor endothelial marker 8 (TEM8) is one of two known proteinaceous cell surface anthrax toxin receptors. A metal ion dependent adhesion site (MIDAS) present in the integrin-like inserted (I) domain of ANTXR1 mediates the binding of the anthrax toxin subunit, protective antigen (PA). ...

journal_title：Biochemistry

authors： Ramey JD,Villareal VA,Ng C,Ward SC,Xiong JP,Clubb RT,Bradley KA

更新日期：2010-08-31 00:00:00

abstract：:Structures of several voltage-gated sodium (NaV) channels from bacteria have been determined recently, but the same feat might not be achieved for the mammalian counterparts in the near future. Thus, at present, computational studies of the mammalian NaV channels have to be performed using homology models based on the...

journal_title：Biochemistry

authors： Patel D,Mahdavi S,Kuyucak S

更新日期：2016-03-29 00:00:00

abstract：:The complete amino acid sequence of human liver NADPH-cytochrome P-450 reductase has been determined by microsequence analysis and mass spectrometry. The total sequence consists of 676 amino acids initiated by an amino-terminal acetyl group. There is no evidence for posttranslational modifications, including Asn-linke...

journal_title：Biochemistry

authors： Haniu M,McManus ME,Birkett DJ,Lee TD,Shively JE

更新日期：1989-10-17 00:00:00

abstract：:Aromatic amino acids often flank the transmembrane alpha helices of integral membrane proteins. By favoring locations within the membrane-water interface of the lipid bilayer, aromatic residues Trp, Tyr, and sometimes Phe may serve as anchors to help stabilize a transmembrane orientation. In this work, we compare the ...

journal_title：Biochemistry

authors： Sparks KA,Gleason NJ,Gist R,Langston R,Greathouse DV,Koeppe RE 2nd

更新日期：2014-06-10 00:00:00