Abstract:
:Fluorescence-detected circular dichroism (FDCD) spectra are reported for ethidium in Escherichia coli cells and bound to E. coli DNA in vitro. FDCD bands are observed at 325 and 385 nm. These bands change amplitude as the ethidium to DNA ratio changes. Spectra are similar for in vivo and in vitro measurements. However, the bands at 325 and 385 nm disappear when ethidium binds to macromolecules without intercalating between base pairs. The results demonstrate that FDCD spectra can be measured in cell suspensions and indicate that ethidium binds to nucleic acids in E. coli cells by intercalation.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lamos ML,Turner DHdoi
10.1021/bi00333a001subject
Has Abstractpub_date
1985-06-04 00:00:00pages
2819-22issue
12eissn
0006-2960issn
1520-4995journal_volume
24pub_type
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