Structural analysis of O2'-methyl-5-carbamoylmethyluridine, a newly discovered constituent of yeast transfer RNA.

abstract：:Terminase is an enzyme from bacteriophage lambda that is required for insertion of the viral genome into an empty pro-capsid. This enzyme is composed of the viral proteins gpNu1 (20.4 kDa) and gpA (73.3 kDa) in a holoenzyme complex. Current models for terminase assembly onto DNA suggest that gpNu1 binds to three repea...

journal_title：Biochemistry

authors： Yang Q,de Beer T,Woods L,Meyer JD,Manning MC,Overduin M,Catalano CE

更新日期：1999-01-05 00:00:00

abstract：:All phosphagen kinases contain a conserved cysteine residue which has been shown by crystallographic studies, on both creatine kinase and arginine kinase, to be located in the active site. There are conflicting reports as to whether this cysteine is essential for catalysis. In this study we have used site-directed mut...

journal_title：Biochemistry

authors： Wang PF,McLeish MJ,Kneen MM,Lee G,Kenyon GL

更新日期：2001-10-02 00:00:00

abstract：:The human intestinal Caco-2 cell line, described as enterocyte-like in a number of studies, was examined for its ability to carry out the metabolism of vitamin A normally required in the absorptive process. Caco-2 cells contained cellular retinol-binding protein II, a protein which is abundant in human villus-associat...

journal_title：Biochemistry

authors： Quick TC,Ong DE

更新日期：1990-12-18 00:00:00

abstract：:The cyanobacterium Synechocystis sp. PCC 6803 (S6803) expresses a two-on-two globin in which His46 (distal side) and His70 (proximal) function as heme iron axial ligands. His46 can be displaced by O2, CO, and CN-, among others, whereas His70 is not labile under native conditions. The residue preceding the proximal his...

journal_title：Biochemistry

authors： Knappenberger JA,Kuriakose SA,Vu BC,Nothnagel HJ,Vuletich DA,Lecomte JT

更新日期：2006-09-26 00:00:00

abstract：:The ZFY protein is a member of one of the most interesting classes of polydactyl zinc finger proteins. It has a domain of 13 tandem zinc fingers that is organized with an internal repeat of odd-even finger pairs. It has been proposed that each finger pair interacts with six base pairs within a turn of the double helix...

journal_title：Biochemistry

authors： Grants J,Flanagan E,Yee A,Romaniuk PJ

更新日期：2010-02-02 00:00:00

abstract：:From the larger set of 191 variants at all the variable contact positions in the turkey ovomucoid third domain, we selected a subset that consists of Asp, Glu, His, and Lys residues at eight of the nine contiguous P6-P3' positions (residues 13-21), the exception being P3-Cys16 which is involved in a conserved disulfid...

journal_title：Biochemistry

authors： Song J,Laskowski M Jr,Qasim MA,Markley JL

更新日期：2003-03-18 00:00:00

abstract：:Plasma high-density lipoprotein and apolipoprotein AI (apoAI) levels are suppressed by tumor necrosis factor alpha. To determine the molecular mechanisms responsible for the effect of TNF alpha on the apoAI promoter activity, HepG2 cells were exposed to both genetic and pharmacological modulators of TNF alpha-mediated...

journal_title：Biochemistry

authors： Beers A,Haas MJ,Wong NC,Mooradian AD

更新日期：2006-02-21 00:00:00

abstract：:Heme-regulated eIF2alpha kinase [heme-regulated inhibitor (HRI)] plays a critical role in the regulation of protein synthesis by heme iron. The kinase active site is located in the C-terminal domain, whereas the N-terminal domain is suggested to regulate catalysis in response to heme binding. Here, we found that the r...

journal_title：Biochemistry

authors： Miksanova M,Igarashi J,Minami M,Sagami I,Yamauchi S,Kurokawa H,Shimizu T

更新日期：2006-08-15 00:00:00

abstract：:Aminopeptidases catalyze the hydrolysis of amino acid residues from the amino terminus of peptide substrates. They are found in most cells and tissues, and their activity has been implicated in myriad fundamental biochemical and physiological processes. Nevertheless, little is known about the structure of the aminopep...

journal_title：Biochemistry

authors： Taylor A,Peltier CZ,Jahngen EG Jr,Laxman E,Szewczuk Z,Torre FJ

更新日期：1992-04-28 00:00:00

abstract：:Heme-derived linear tetrapyrroles (phytobilins) in phycobiliproteins and phytochromes perform critical light-harvesting and light-sensing roles in oxygenic photosynthetic organisms. A key enzyme in their biogenesis, phycocyanobilin:ferredoxin oxidoreductase (PcyA), catalyzes the overall four-electron reduction of bili...

journal_title：Biochemistry

authors： Kohler AC,Gae DD,Richley MA,Stoll S,Gunn A,Lim S,Martin SS,Doukov TI,Britt RD,Ames JB,Lagarias JC,Fisher AJ

更新日期：2010-07-27 00:00:00

abstract：:The discovery of three mutants in the -synuclein (alphaSyn) gene and the identification of alphaSyn as the major component of Lewy body have opened a new field for understanding the pathogenesis of Parkinson's disease (PD). AlphaSyn is a natively unfolded protein with unknown function and unspecified conformational he...

journal_title：Biochemistry

authors： Jiang C,Chang JY

更新日期：2007-01-16 00:00:00

abstract：:The light-induced QA-/QA FTIR difference spectrum of the photoreduction of the primary quinone (QA) in reaction centers (RCs) from Rhodobacter sphaeroides exhibits a set of complex differential bands between 1750 and 1715 cm(-1). Several of these features correspond in frequency to bands that bleach in the HA-/HA FTIR...

journal_title：Biochemistry

authors： Breton J,Nabedryk E,Allen JP,Williams JC

更新日期：1997-04-15 00:00:00

abstract：:The interactions of Pr(III) and Eu(III) with specifically nitrated derivatives of the basic bovine pancreatic trypsin inhibitor have been studied using optical spectroscopy and nuclear magnetic resonance (NMR) at 250 and 270 MHz. Stability constants for proton and metal binding to nitrotyrosines 10 and 21 determined o...

journal_title：Biochemistry

authors： Marinetti TD,Snyder GH,Sykes BD

更新日期：1977-02-22 00:00:00

abstract：:Several recent studies have provided evidence that LIN28, a cytoplasmic RNA-binding protein, inhibits the biogenesis of members of the let-7 microRNA family at the Dicer step in both mammals and Caenorhabditis elegans. However, the precise mechanism of inhibition is still poorly understood. Here we report on an in vit...

journal_title：Biochemistry

authors： Lightfoot HL,Bugaut A,Armisen J,Lehrbach NJ,Miska EA,Balasubramanian S

更新日期：2011-09-06 00:00:00

abstract：:We show here that expression of chromogranins in non-neuroendocrine NIH3T3 cells significantly increased the amount of IP(3)-mediated intracellular Ca(2+) mobilization in these cells, whereas suppression of them in neuroendocrine PC12 cells decreased the amount of mobilized Ca(2+). We have therefore investigated the r...

journal_title：Biochemistry

authors： Huh YH,Jeon SH,Yoo JA,Park SY,Yoo SH

更新日期：2005-04-26 00:00:00

abstract：:NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...

journal_title：Biochemistry

authors： Eibl C,Grigoriu S,Hessenberger M,Wenger J,Puehringer S,Pinheiro AS,Wagner RN,Proell M,Reed JC,Page R,Diederichs K,Peti W

更新日期：2012-09-18 00:00:00

abstract：:Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has a well-established causative role in cancer metastasis. However, little is known about its natural substrates, pathways, and biological functions, and only a few protein substrates have been sugges...

journal_title：Biochemistry

authors： McParland V,Varsano G,Li X,Thornton J,Baby J,Aravind A,Meyer C,Pavic K,Rios P,Köhn M

更新日期：2011-09-06 00:00:00

abstract：:The N-terminus of the major polypeptide component of the light-harvesting complex (LHC) from the brown alga Laminaria saccharina is blocked. Two partial sequences, one near the N-terminus and the other near the C-terminus, have been obtained by chemical cleavage with acetic acid and N-chlorosuccinimide. Four peptides ...

journal_title：Biochemistry

authors： Douady D,Rousseau B,Caron L

更新日期：1994-03-22 00:00:00

abstract：:Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...

journal_title：Biochemistry

authors： Riccardi D,König P,Guo H,Cui Q

更新日期：2008-02-26 00:00:00

abstract：:Resonance Raman and UV-visible absorption spectra show that nickel(II) microperoxidase-11 (NiMP-11) is four-coordinate in aqueous solution in the pH range from 1.0 to 13.0. In aqueous solutions of NiMP-11 in the absence of cetyltrimethylammonium bromide (CTAB), NiMP-11 is aggregated. In CTAB micellar solutions, where ...

journal_title：Biochemistry

authors： Ma JG,Vanderkooi JM,Zhang J,Jia SL,Shelnutt JA

更新日期：1999-03-02 00:00:00

abstract：:In this paper the interaction of cytoplasmic CopZ and the N-terminal domain of the CopA ATPase from Bacillus subtilis has been studied by NMR through (15)N-(1)H HSQC experiments in order to understand the role of the two proteins in the whole copper trafficking mechanism of the bacteria. It appears that the two protei...

journal_title：Biochemistry

authors： Banci L,Bertini I,Ciofi-Baffoni S,Del Conte R,Gonnelli L

更新日期：2003-02-25 00:00:00

abstract：:The function of the GM2 activator protein is to act as a substrate-specific cofactor in the hydrolysis of GM2 ganglioside by beta-hexosaminidase A. Mutations in the gene encoding it result in the AB variant form of GM2 gangliosidosis. One such mutation, Cys138 Arg, results in the mutant protein being retained and degr...

journal_title：Biochemistry

authors： Xie B,Rigat B,Smiljanic-Georgijev N,Deng H,Mahuran D

更新日期：1998-01-20 00:00:00

abstract：:The existence of two conformational states of concanavalin A (Con A) with different metal ion binding properties has been recently demonstrated (Brown, R. D., Brewer, C. F., & Koenig, S. H. (1977) Biochemistry 16, 3883). Introduction of Mn2+ to the S1 site and Ca2+ to the S2 site of apo-Con A was shown to induce a con...

journal_title：Biochemistry

authors： Koenig SH,Brewer CF,Brown RD 3rd

更新日期：1978-10-03 00:00:00

abstract：:The kinetics of the tryptophan fluorescence enhancement that occurs when myofibrils (rabbit psoas) are mixed with Mg-ATP were studied by stopped-flow in different solvents (water, 40% ethylene glycol, 20% methanol) at 4 degrees C. Under relaxing conditions (low Ca(2+)) in water (mu = 0.16 M, pH 7.4) and at high ATP co...

journal_title：Biochemistry

authors： Stehle R,Lionne C,Travers F,Barman T

更新日期：2000-06-27 00:00:00

abstract：:Endonuclease V from bacteriophage T4 is a well characterized enzyme that initiates the repair of ultraviolet light induced pyrimidine dimers. Scission of the phosphodiester backbone between the pyrimidines within a dimer, or 3' to an abasic (AP) site, occurs by a beta-elimination mechanism. In addition, high concentra...

journal_title：Biochemistry

authors： Latham KA,Lloyd RS

更新日期：1995-07-11 00:00:00

abstract：:Tumor suppressor Nore1, its acronym coming from novel Ras effector, is one of the 10 members of the Rassf (Ras association domain family) protein family that have been identified. It is expressed as two mRNA splice variants, Nore1A and a shorter isoform, Nore1B. It forms homo- and heterocomplexes through its C-termina...

journal_title：Biochemistry

authors： Makbul C,Constantinescu Aruxandei D,Hofmann E,Schwarz D,Wolf E,Herrmann C

更新日期：2013-02-12 00:00:00

abstract：:The intercellular environment is known to be very different from the environment where most of the elementary biological processes are studied in the laboratory. As a result, there was a considerable effort on cell mimicking either by confinement or by introducing macromolecular crowding. In the present study, dextran...

journal_title：Biochemistry

authors： Das N,Sen P

更新日期：2018-10-23 00:00:00

abstract：:Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...

journal_title：Biochemistry

authors： Jumblatt JE,Schlup V,Burger MM

更新日期：1980-03-04 00:00:00

abstract：:Recent studies implicate hydrogen sulfide (H2S) oxidation as an important aspect of bacterial antibiotic resistance and sulfide homeostasis. The cst operon of the major human pathogen Staphylococcus aureus is induced by exogenous H2S stress and encodes enzymes involved in sulfide oxidation, including a group I flavopr...

journal_title：Biochemistry

authors： Shen J,Peng H,Zhang Y,Trinidad JC,Giedroc DP

更新日期：2016-11-29 00:00:00

abstract：:The functional role of the Ca (2+) ion in the oxygen-evolving complex of photosystem II is not yet clear. Current models explain why the redox cycle of the complex would be interrupted after the S 3 state without Ca (2+), but the literature shows that it is interrupted after the S 2 state. Reinterpretation of the lite...

journal_title：Biochemistry

authors： Miqyass M,Marosvölgyi MA,Nagel Z,Yocum CF,van Gorkom HJ

更新日期：2008-07-29 00:00:00