Investigation of quercetin binding sites on chloroplast coupling factor 1.

abstract：:R67 dihydrofolate reductase (DHFR) is a novel homotetrameric protein that possesses 222 symmetry and a single, voluminous active site pore. This symmetry poses numerous limitations on catalysis; for example, two dihydrofolate (DHF) molecules or two NADPH molecules, or one substrate plus one cofactor can bind. Only the...

journal_title：Biochemistry

authors： Chopra S,Lynch R,Kim SH,Jackson M,Howell EE

更新日期：2006-05-30 00:00:00

abstract：:Monomeric sarcosine oxidase is a flavoenzyme that catalyzes the oxidation of the methyl group in sarcosine (N-methylglycine). Rapid reaction kinetic studies under anaerobic conditions at pH 8.0 show that the enzyme forms a charge transfer Michaelis complex with sarcosine (E-FAD(ox).sarcosine) that exhibits an intense ...

journal_title：Biochemistry

authors： Zhao G,Jorns MS

更新日期：2006-05-16 00:00:00

abstract：:The five-membered nitrogen plus heteroatom rings known as azolines or in their oxidized form as azoles are very common in natural products and drugs. The oxidation of thiazoline to thiazole in the cyanobactin class of natural products is one of the several important transformations that are known to alter the biologic...

journal_title：Biochemistry

authors： Gao S,Ge Y,Bent AF,Schwarz-Linek U,Naismith JH

更新日期：2018-10-16 00:00:00

abstract：:Putative physiological partners of the [2Fe-2S] ferredoxin from Clostridium pasteurianum have been searched by running crude soluble extracts of this bacterium through an affinity column to which the [2Fe-2S] ferredoxin had been covalently bound. Subsequent washing of the column with buffers of increasing ionic streng...

journal_title：Biochemistry

authors： Golinelli MP,Gagnon J,Meyer J

更新日期：1997-09-30 00:00:00

abstract：:Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides and 2'-deoxyribonucleosides, yielding the purine base and (2'-deoxy)ribose 1-phosphate as products. While this enzyme has been extensively studied, several questions with respect to the catalytic mechanism have rema...

journal_title：Biochemistry

authors： Isaksen GV,Hopmann KH,Åqvist J,Brandsdal BO

更新日期：2016-04-12 00:00:00

abstract：:X-ray absorption spectroscopy has been used to investigate binding of selenohomocysteine to cobalamin-independent (MetE) and cobalamin-dependent (MetH) methionine synthase enzymes of Escherichia coli. We have shown previously [Peariso et al. (1998) J. Am. Chem. Soc. 120, 8410-8416] that the Zn sites in both enzymes sh...

journal_title：Biochemistry

authors： Peariso K,Zhou ZS,Smith AE,Matthews RG,Penner-Hahn JE

更新日期：2001-01-30 00:00:00

abstract：:The addition of 10(-9) M insulin to a suspension of rat liver plasma membranes increases the overall lipid microviscosity, eta, by about 10--20%. The effect is confined to physiological concentrations of the hormone and is highly specific. The specificity was demonstrated in experiments where insulin analogues were ad...

journal_title：Biochemistry

authors： Luly P,Shinitzky M

更新日期：1979-02-06 00:00:00

abstract：:In the native calcium-binding protein calbindin D9k (M(r) 8.700; 75aa; 2 EF-hands), the backbone carbonyl oxygen of Glu60 coordinates the Ca2+ ion in the C-terminal site (site II). The carboxylate group of the same residue forms a hydrogen bond to a water molecule that constitutes a Ca2+ ligand in the N-terminal site ...

journal_title：Biochemistry

authors： Linse S,Bylsma NR,Drakenberg T,Sellers P,Forsén S,Thulin E,Svensson LA,Zajtzeva I,Zajtsev V,Marek J

更新日期：1994-10-18 00:00:00

abstract：:We review signaling by reactive oxygen species, which is emerging as a major physiological process. However, among the reactive oxygen species, H(2)O(2) best fulfills the requirements of being a second messenger. Its enzymatic production and degradation, along with the requirements for the oxidation of thiols by H(2)O...

journal_title：Biochemistry

authors： Forman HJ,Maiorino M,Ursini F

更新日期：2010-02-09 00:00:00

abstract：:CYP2C19 is selective for the 4'-hydroxylation of S-mephenytoin while the highly similar CYP2C9 has little activity toward this substrate. To identify critical amino acids determining the specificity of human CYP2C19 for S-mephenytoin 4'-hydroxylation, we constructed chimeras by replacing portions of CYP2C9 containing ...

journal_title：Biochemistry

authors： Tsao CC,Wester MR,Ghanayem B,Coulter SJ,Chanas B,Johnson EF,Goldstein JA

更新日期：2001-02-20 00:00:00

abstract：:Human lysine demethylase KDM5A is a chromatin-modifying enzyme associated with transcriptional regulation, because of its ability to catalyze removal of methyl groups from methylated lysine 4 of histone H3 (H3K4me3). Amplification of KDM5A is observed in many cancers, including breast cancer, prostate cancer, hepatoce...

journal_title：Biochemistry

authors： Petronikolou N,Longbotham JE,Fujimori DG

更新日期：2020-02-11 00:00:00

abstract：:Multidimensional heteronuclear NMR techniques were applied to study the phosphotransfer domain, residues 1-134, of the histidine kinase CheA, from Escherichia coli, which contains the site of autophosphorylation, His48. Assignments of the backbone amide groups and side chain protons are nearly complete. Our studies sh...

journal_title：Biochemistry

authors： Zhou H,Lowry DF,Swanson RV,Simon MI,Dahlquist FW

更新日期：1995-10-24 00:00:00

abstract：:The locations of RNA-RNA cross-links in 16S rRNA were determined after in vivo incorporation of 4-thiouridine (s(4)U) into RNA in a strain of Escherichia coli deficient in pyrimidine synthesis and irradiation at >320 nm. This was done as an effort to find RNA cross-links different from UVB-induced cross-links that wou...

journal_title：Biochemistry

authors： Nanda K,Wollenzien P

更新日期：2004-07-20 00:00:00

abstract：:By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...

journal_title：Biochemistry

authors： Singh AK,Ekka MK,Kaushik A,Pandya V,Singh RP,Banerjee S,Mittal M,Singh V,Kumaran S

更新日期：2017-09-19 00:00:00

abstract：:Heparin lyases are valuable tools for generating oligosaccharide fragments and in sequence determination of heparan sulfate (HS). Heparin lyase III is known to cleave the linkages between N-acetylglucosamine (GlcNAc) or N-sulfated glucosamine (GlcNS) and glucuronic acid (GlcA) as the primary sites and the linkages bet...

journal_title：Biochemistry

authors： Chai W,Leteux C,Westling C,Lindahl U,Feizi T

更新日期：2004-07-06 00:00:00

abstract：:The binding site of cyclosporin A to P-glycoprotein was characterized by using a multidrug-resistant Chinese hamster ovary cell line. P-glycoprotein photolabeled with diazirine-cyclosporin A analogue was purified by a two-step process involving continuous elution electrophoresis followed by wheat germ agglutinin-agaro...

journal_title：Biochemistry

authors： Demeule M,Laplante A,Murphy GF,Wenger RM,Béliveau R

更新日期：1998-12-22 00:00:00

abstract：:tRNase Z, which exists in almost all cells, is believed to be working primarily for tRNA 3' maturation. In Escherichia coli, however, the tRNase Z gene appears to be dispensable under normal growth conditions, and its physiological role is not clear. Here, to investigate a possibility that E. coli tRNase Z cleaves RNA...

journal_title：Biochemistry

authors： Shibata HS,Minagawa A,Takaku H,Takagi M,Nashimoto M

更新日期：2006-05-02 00:00:00

abstract：:Neutral glycosphingolipids and gangliosides in rabbit thymus, spleen, bone marrow, and erythrocyte ghosts were analyzed by conventional chemical and enzymatic procedures and negative ion fast atom bombardment mass spectrometry (FABMS). Thymus gangliosides showed a characteristic composition. Major gangliosides compris...

journal_title：Biochemistry

authors： Iwamori M,Kiguchi K,Kanno J,Kitagawa M,Nagai Y

更新日期：1986-02-25 00:00:00

abstract：:Cytochrome P-450(M-1) [P-450(M-1)] is specifically expressed in adult male rat liver [Yoshioka, H., Morohashi, K., Sogawa, K., Miyata, T., Kawajiri, K., Hirose, T., Inayama, S., Fujii-Kuriyama, Y., & Omura, T. (1987) J. Biol. Chem. 262, 1706-1711]. Isolation and analysis of the gene for P-450(M-1) revealed that the co...

journal_title：Biochemistry

authors： Morishima N,Yoshioka H,Higashi Y,Sogawa K,Fujii-Kuriyama Y

更新日期：1987-12-15 00:00:00

abstract：:Absorbance difference spectra were recorded at 20 degrees C from 30 ns to milliseconds after photolysis of lauryl maltoside suspensions of artificial visual pigments derived from 9-cis isomers of 5-ethylretinal, 8,16-methanoretinal (a 6-s-trans-bicyclic analogue), or 5-demethyl-8-methylretinal. In all three pigments, ...

journal_title：Biochemistry

authors： Szundi I,de Lera AR,Pazos Y,Alvarez R,Oliana M,Sheves M,Lewis JW,Kliger DS

更新日期：2002-02-12 00:00:00

abstract：:The Xenopus borealis somatic 5S ribosomal RNA gene was used as a model system to determine the mutual effects of nucleosome folding and formation of ultraviolet (UV) photoproducts (primarily cis-syn cyclobutane pyrimidine dimers, or CPDs) in chromatin. We analyzed the preferred rotational and translational settings of...

journal_title：Biochemistry

authors： Liu X,Mann DB,Suquet C,Springer DL,Smerdon MJ

更新日期：2000-01-25 00:00:00

abstract：:PII proteins are sensors of alpha-ketoglutarate and adenylylate energy charge that regulate signal transduction proteins, metabolic enzymes, and permeases involved in nitrogen assimilation. Here, purified Escherichia coli PII and two of its receptors, ATase and NRII, were used to study the mechanisms of sensation by P...

journal_title：Biochemistry

authors： Jiang P,Ninfa AJ

更新日期：2009-12-08 00:00:00

abstract：:Fatty acid-induced upregulation of death receptor 5 (DR5) and its cognate ligand, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), promotes hepatocyte lipoapoptosis, which is a key mechanism in the progression of fatty liver disease. Accordingly, inhibition of DR5 signaling represents an attractive str...

journal_title：Biochemistry

authors： Vunnam N,Szymonski S,Hirsova P,Gores GJ,Sachs JN,Hackel BJ

更新日期：2020-10-13 00:00:00

abstract：:The plasma membrane of the Ehrlich ascites tumor cell contains an NADH dehydrogenase. This activity was shown not to be due to contamination by other subcellular membranes. A variety of electron acceptors have been compared as to rate with the following result: ferricyanide greater than cytochrome c greater than cytoc...

journal_title：Biochemistry

authors： Kilberg MS,Christensen HN

更新日期：1979-04-17 00:00:00

abstract：:Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...

journal_title：Biochemistry

authors： Seto R,Takaichi S,Kurihara T,Kishi R,Honda M,Takenaka S,Tsukatani Y,Madigan MT,Wang-Otomo ZY,Kimura Y

更新日期：2020-06-30 00:00:00

abstract：:Redox transitions in the Rhodobacter capsulatus cytochrome bc(1) complex were investigated by perfusion-induced attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with synchronous visible spectroscopy, in both the wild type and a cytochrome c(1) point mutant, M183K, in which the mi...

journal_title：Biochemistry

authors： Iwaki M,Osyczka A,Moser CC,Dutton PL,Rich PR

更新日期：2004-07-27 00:00:00

abstract：:Murine fibroblasts cultured in the presence of fibroblast growth factor-1 express relatively high levels of FR-1, a approximately 36 kDa protein related to the aldo-keto reductase superfamily [Donohue, P. J., Alberts, G. F., Hampton, B. S., Winkles, J. A. (1994) J. Biol. Chem. 269, 8604-8609]. While the crystal struct...

journal_title：Biochemistry

authors： Srivastava S,Harter TM,Chandra A,Bhatnagar A,Srivastava SK,Petrash JM

更新日期：1998-09-15 00:00:00

abstract：:We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micromete...

journal_title：Biochemistry

authors： Soares AS,Engel MA,Stearns R,Datwani S,Olechno J,Ellson R,Skinner JM,Allaire M,Orville AM

更新日期：2011-05-31 00:00:00

abstract：:Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhi...

journal_title：Biochemistry

authors： Hsieh PC,Kowalczyk TH,Phillips NF

更新日期：1996-07-30 00:00:00

abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...

journal_title：Biochemistry

authors： Cotruvo JA,Stubbe J

更新日期：2011-03-15 00:00:00