Acoustically mounted microcrystals yield high-resolution X-ray structures.

Abstract:

:We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Soares AS,Engel MA,Stearns R,Datwani S,Olechno J,Ellson R,Skinner JM,Allaire M,Orville AM

doi

10.1021/bi200549x

subject

Has Abstract

pub_date

2011-05-31 00:00:00

pages

4399-401

issue

21

eissn

0006-2960

issn

1520-4995

journal_volume

50

pub_type

杂志文章