Abstract:
:We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Soares AS,Engel MA,Stearns R,Datwani S,Olechno J,Ellson R,Skinner JM,Allaire M,Orville AMdoi
10.1021/bi200549xsubject
Has Abstractpub_date
2011-05-31 00:00:00pages
4399-401issue
21eissn
0006-2960issn
1520-4995journal_volume
50pub_type
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