Cytochrome P-450 inactivation: structure of the prosthetic heme adduct with propyne.

abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...

journal_title：Biochemistry

authors： Sun M,Leyh TS

更新日期：2006-09-26 00:00:00

abstract：:Translational initiation factor IF-2 is involved in a multistep pathway leading to the synthesis of the first peptide bond. IF-2 is a guanine nucleotide binding protein (G-protein) and catalyzes GTP hydrolysis in the presence of ribosomes. According to sequence homologies with other G-proteins, particularly EF-Tu, a t...

journal_title：Biochemistry

authors： Vachon G,Laalami S,Grunberg-Manago M,Julien R,Cenatiempo Y

更新日期：1990-10-16 00:00:00

abstract：:The glucose derivative, 2,2,6,6-tetramethylpiperidine-1-oxylglucose (TEMPO-glucose) was synthesized and examined for its ability to substitute for glucose as a substrate for the intestinal brush border membrane Na+/glucose cotransporter. TEMPO-glucose inhibited Na(+)-dependent phlorizin binding with an apparent KI of ...

journal_title：Biochemistry

authors： Peerce BE

更新日期：1991-04-30 00:00:00

abstract：:Histone deacetylase 8 (HDAC8) is a well-characterized member of the class I acetyl-lysine deacetylase (HDAC) family. Previous work has shown that the efficiency of HDAC8-catalyzed deacetylation of a methylcoumarin peptide varies depending on the identity of the divalent metal ion in the HDAC8 active site. Here we demo...

journal_title：Biochemistry

authors： Castaneda CA,Lopez JE,Joseph CG,Scholle MD,Mrksich M,Fierke CA

更新日期：2017-10-24 00:00:00

abstract：:Prokaryotic promoters have been extensively studied to relate sequence features to promoter function. Here we examine the relationship between double-helix stability and promoter activity. The double-helix stability is evaluated from sequence data by free energy computation, based on reported values of dinucleotide fr...

journal_title：Biochemistry

authors： Margalit H,Shapiro BA,Nussinov R,Owens J,Jernigan RL

更新日期：1988-07-12 00:00:00

abstract：:β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natur...

journal_title：Biochemistry

authors： Mojica MF,Mahler SG,Bethel CR,Taracila MA,Kosmopoulou M,Papp-Wallace KM,Llarrull LI,Wilson BM,Marshall SH,Wallace CJ,Villegas MV,Harris ME,Vila AJ,Spencer J,Bonomo RA

更新日期：2015-05-26 00:00:00

abstract：:Histidinol-phosphate aminotransferase (HspAT) is a key enzyme on the histidine biosynthetic pathway. HspAT catalyzes the transfer of the amino group of L-histidinol phosphate (Hsp) to 2-oxoglutarate to form imidazole acetol phosphate (IAP) and glutamate. Thus, HspAT recognizes two kinds of substrates, Hsp and glutamat...

journal_title：Biochemistry

authors： Haruyama K,Nakai T,Miyahara I,Hirotsu K,Mizuguchi H,Hayashi H,Kagamiyama H

更新日期：2001-04-17 00:00:00

abstract：:3-(4-Azido-2,3,5,6-tetrafluorobenzoyl)-6-hydroxy-2-(4- hydroxyphenyl)benzo[b]thiophene 1 (tetrafluoroaryl azide, TFAA) and its protio analogue 3-(4-azidobenzoyl)-6- hydroxy-2-(4-hydroxyphenyl)benzo[b]thiophene 2 (protioaryl azide, PAA), photoaffinity labeling (PAL) reagents for the estrogen receptor (ER), have been pr...

journal_title：Biochemistry

authors： Pinney KG,Carlson KE,Katzenellenbogen BS,Katzenellenbogen JA

更新日期：1991-03-05 00:00:00

abstract：:Indolotryptoline natural products represent a small family of structurally unique chromopyrrolic acid-derived antiproliferative agents. Like many prospective anticancer agents before them, the exploration of their potential clinical utility has been hindered by the limited information known about their mechanism of ac...

journal_title：Biochemistry

authors： Chang FY,Kawashima SA,Brady SF

更新日期：2014-11-18 00:00:00

abstract：:Purine C-8 tritium-labeling rates have been measured at specific sites in Escherichia coli tRNAIle and tRNA2Tyr. The results are compared with those obtained for yeast tRNAPhe (preceding paper(Gamble et al., 1976)). The tRNAIle and tRNAPhe fall into the same general class of tRNA structures, while tRNA2Tyr is in a dif...

journal_title：Biochemistry

authors： Schoemaker HJ,Gamble RC

更新日期：1976-06-29 00:00:00

abstract：:Base excision repair is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). Base excision repair requires the coordinated action of several repair and ancillary proteins, the impairment of which can lead to genetic instability. Using a protein-DNA cross-linking a...

journal_title：Biochemistry

authors： Parsons JL,Dianova II,Allinson SL,Dianov GL

更新日期：2005-08-09 00:00:00

abstract：:The structural and motional properties of membrane lipids in various physical states and macroscopic organizations are elucidated by 1H NMR dipolar echo decay spectroscopy (DECODE). Multilamellar lipid dispersions in the gel (L beta') and liquid-crystalline (L alpha) states and a nonbilayer, hexagonal (HII) phase are ...

journal_title：Biochemistry

authors： Janes N,Rubin E,Taraschi TF

更新日期：1990-09-11 00:00:00

abstract：:Nine monoclonal antibodies were used to test calcium and EDTA effects on the molecular conformation of ventricular VLC2 within myosin. Antibody epitopes were located in six domains of VLC2 using recombinant proteins. The apparent association constants of these antibodies were measured in solution in the presence of ca...

journal_title：Biochemistry

authors： Eldin P,Cathiard AM,Anoal M,Léger J,Mornet D,Léger JJ,Cornillon B

更新日期：1994-10-25 00:00:00

abstract：:Signal transducer and activator of transcription factors (STATs) are proteins that can translocate into the nucleus, bind DNA, and activate gene transcription. STAT proteins play a crucial role in cell proliferation, apoptosis, and differentiation. The prevalent view is that STAT proteins are able to form dimers and b...

journal_title：Biochemistry

authors： Sgrignani J,Olsson S,Ekonomiuk D,Genini D,Krause R,Catapano CV,Cavalli A

更新日期：2015-09-08 00:00:00

abstract：:The purified PBS2 phage-coded inhibitor of uracil-DNA glycosylase (Ura-DNA glycosylase) from Bacillus subtilis has been tested for its ability to inhibit this enzyme isolated from other prokaryotic and from eukaryotic sources. In addition, the inhibitor has been assayed for its effect on DNA glycosylases specific for ...

journal_title：Biochemistry

authors： Karran P,Cone R,Friedberg EC

更新日期：1981-10-13 00:00:00

abstract：:Gemcitabine 5'-diphosphate (F(2)CDP) is a potent inhibitor of ribonucleotide reductases (RNRs), enzymes that convert nucleotides (NDPs) to deoxynucleotides and are essential for DNA replication and repair. The Escherichia coli RNR, an alpha2beta2 complex, when incubated with 1 equiv of F(2)CDP catalyzes the release of...

journal_title：Biochemistry

authors： Artin E,Wang J,Lohman GJ,Yokoyama K,Yu G,Griffin RG,Bar G,Stubbe J

更新日期：2009-12-15 00:00:00

abstract：:The characteristics of tetrahydrobiopterin (H4biopterin) binding to pteridine-free recombinant macrophage inducible nitric oxide synthase expressed in Escherichia coli were investigated with a special focus given to effects caused by 2,4-diamino-5,6,7, 8-tetrahydro-6-(l-erythro-1,2-dihydroxypropyl)pteridine (4-amino-H...

journal_title：Biochemistry

authors： Mayer B,Wu C,Gorren AC,Pfeiffer S,Schmidt K,Clark P,Stuehr DJ,Werner ER

更新日期：1997-07-08 00:00:00

abstract：:Histone tails in nucleosomes play critical roles in regulation of many biological processes, including chromatin compaction, transcription, and DNA repair. Moreover, post-translational modifications, notably lysine acetylation, are crucial to these functions. While the tails have been intensively studied, how the stru...

journal_title：Biochemistry

authors： Fu I,Cai Y,Geacintov NE,Zhang Y,Broyde S

更新日期：2017-04-11 00:00:00

abstract：:Src homology 2 (SH2) domains are phosphotyrosine- (pY-) binding modules found in a variety of signal-transducing proteins and constitute an important class of drug targets for the treatment of signaling related diseases/conditions. To date, a large number of peptidic as well as nonpeptidic SH2 domain inhibitors have b...

journal_title：Biochemistry

authors： Park J,Fu H,Pei D

更新日期：2003-05-06 00:00:00

abstract：:We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesi...

journal_title：Biochemistry

authors： Sidik K,Smerdon MJ

更新日期：1990-08-14 00:00:00

abstract：:An investigation into the kinetics and regulatory properties of the type-1 phosphofructokinase (PFK) from the extreme thermophile Thermus thermophilus (TtPFK) reveals an enzyme that is inhibited by PEP and activated by ADP by modifying the affinity exhibited for the substrate fructose 6-phosphate (Fru-6-P) in a manner...

journal_title：Biochemistry

authors： McGresham MS,Lovingshimer M,Reinhart GD

更新日期：2014-01-14 00:00:00

abstract：:Aminoimidazole riobnucleotide carboxylase, the sixth step in the purine biosynthetic pathway, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to carboxyaminoimidazole ribonucleotide (CAIR). The gene products of the purE and purK genes (PurE and PurK, respectively) thought to be responsible for this act...

journal_title：Biochemistry

authors： Meyer E,Leonard NJ,Bhat B,Stubbe J,Smith JM

更新日期：1992-06-02 00:00:00

abstract：:Most nuclear receptors recognize the same consensus hexameric sequence, AGGTCA. An important question has been how the various members of this transcription factor family distinguish identity features in these closely related DNA sites. We determined structures from several crystal forms of the RevErb-DNA complex and ...

journal_title：Biochemistry

authors： Sierk ML,Zhao Q,Rastinejad F

更新日期：2001-10-30 00:00:00

abstract：:Toc34 is a transmembrane protein located in the outer envelope membrane of chloroplasts and involved in transit peptide recognition. The cytosolic region of Toc34 reveals 34% alpha-helical and 26% beta-strand structure and is stabilized by intramolecular electrostatic interaction. Toc34 binds both chloroplast preprote...

journal_title：Biochemistry

authors： Schleiff E,Soll J,Sveshnikova N,Tien R,Wright S,Dabney-Smith C,Subramanian C,Bruce BD

更新日期：2002-02-12 00:00:00

abstract：:We investigated the effects of MA-5, a human-specific monoclonal antibody to the insulin receptor alpha-subunit, on transmembrane signaling in cell lines transfected with and expressing both normal human insulin receptors and receptors mutated in their beta-subunit tyrosine kinase domains. In cell lines expressing nor...

journal_title：Biochemistry

authors： Sung CK,Han XL,Brunetti A,Maddux B,Yamamoto-Honda R,Goldfine ID

更新日期：1992-01-14 00:00:00

abstract：:By following the kinetics of abortive and productive synthesis in single-round transcription assays, we confirm the existence of two general classes of initial transcribing complexes (ITCs), which we term "productive ITC" and "unproductive ITC". The productive ITCs are able to escape from the promoter rapidly to produ...

journal_title：Biochemistry

authors： Vo NV,Hsu LM,Kane CM,Chamberlin MJ

更新日期：2003-04-08 00:00:00

abstract：:Base excision repair (BER) is the major corrective pathway for most spontaneous, oxidative, and alkylation DNA base and sugar damage. X-ray cross-complementing 1 (XRCC1) has been suggested to function at nearly every step of this repair process, primarily through direct protein-protein interactions. Using whole cell e...

journal_title：Biochemistry

authors： Wong HK,Kim D,Hogue BA,McNeill DR,Wilson DM 3rd

更新日期：2005-11-01 00:00:00

abstract：:The steroidogenic acute regulatory protein (StAR) facilitates the movement of cholesterol from the outer to inner mitochondrial membrane in adrenal and gonadal cells, fostering steroid biosynthesis. MLN64 is a 445-amino acid protein of unknown function. When 218 amino-terminal residues of MLN-64 are deleted, the resul...

journal_title：Biochemistry

authors： Bose HS,Whittal RM,Huang MC,Baldwin MA,Miller WL

更新日期：2000-09-26 00:00:00

abstract：:The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...

journal_title：Biochemistry

authors： Stöckel J,Safar J,Wallace AC,Cohen FE,Prusiner SB

更新日期：1998-05-19 00:00:00

abstract：:The endogenous phosphoserine residue in porcine pepsinogen has been titrated with use of phosphorus-31 nuclear magnetic resonance (31P NMR). It has an observed pKa2 of 6.7 and a narrow line width (congruent to 10 Hz). The phosphate can be readily removed by an acid phosphatase from potato; however, it is resistant to ...

journal_title：Biochemistry

authors： Williams SP,Bridger WA,James MN

更新日期：1986-10-21 00:00:00