Correlations of crystal structures of DNA oligonucleotides with enantioselective recognition by Rh(phen)2phi3+: probes of DNA propeller twisting in solution.

abstract：:The vitamin K epoxide reductase (VKORC1) enzyme is of primary importance in many physiological processes, i.e., blood coagulation, energy metabolism, and arterial calcification prevention, due to its role in the vitamin K cycle. Indeed, VKORC1 catalyzes reduction of vitamin K epoxide to quinone and then to hydroquinon...

journal_title：Biochemistry

authors： Chatron N,Abi Khalil R,Benoit E,Lattard V

更新日期：2020-04-07 00:00:00

abstract：:A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...

journal_title：Biochemistry

authors： Kealey JT,Santi DV

更新日期：1991-10-08 00:00:00

abstract：:Following platelet activation, platelets undergo a dramatic shape change mediated by the actin cytoskeleton and accompanied by secretion of granule contents. While the actin cytoskeleton is thought to influence platelet granule secretion, the mechanism for this putative regulation is not known. We found that disruptio...

journal_title：Biochemistry

authors： Woronowicz K,Dilks JR,Rozenvayn N,Dowal L,Blair PS,Peters CG,Woronowicz L,Flaumenhaft R

更新日期：2010-06-01 00:00:00

abstract：:Using a simple model for a biological membrane we examine cation-induced gel phase formation and the depletion of polypeptide from the gel phase. The model system consists of vesicles of phosphatidic acid and phosphatidylcholine which contain gramicidin A'. By use of electron spin resonance to monitor lipid phase beha...

journal_title：Biochemistry

authors： Feigenson GW

更新日期：1983-06-21 00:00:00

abstract：:Thermal transitions of type I collagen fibrils were investigated by differential scanning calorimetry and spectrophotometry of turbidity within a wide range of external conditions. The advanced microcalorimeter allowed us to carry out the measurements at low concentrations of collagen (0.15-0.3 mg/mL). At these concen...

journal_title：Biochemistry

authors： Tiktopulo EI,Kajava AV

更新日期：1998-06-02 00:00:00

abstract：:Electron spin echo envelope modulation (ESEEM) spectroscopy has been applied to the determination of the number of water molecules coordinated to the metal in the binary complex of staphylococcal nuclease with Mn2+, to the ternary enzyme-Mn2+-3',5'-pdTp complex, and to ternary complexes of a number of mutant enzymes i...

journal_title：Biochemistry

authors： Serpersu EH,McCracken J,Peisach J,Mildvan AS

更新日期：1988-10-18 00:00:00

abstract：:Crystallographic studies have demonstrated two flavin conformations for p-hydroxybenzoate hydroxylase (PHBH) [Gatti, D. L., Palfey, B. A. , Lah, M. S., Entsch, B., Massey, V., Ballou, D. P., & Ludwig, M. L. (1994) Science 266, 110-114. Schreuder, H. A., Mattevi, A., Obmolova, G., Kalk, K. H., Hol, W. G. J., van der Bo...

journal_title：Biochemistry

authors： Palfey BA,Ballou DP,Massey V

更新日期：1997-12-16 00:00:00

abstract：:The microscopic interactions and mechanisms leading to nascent protein folding events are generally unknown. While such short time-scale events are difficult to study experimentally, molecular dynamics simulations of peptides can provide a useful model for studying events related to protein folding initiation. Recentl...

journal_title：Biochemistry

authors： Karpen ME,Tobias DJ,Brooks CL 3rd

更新日期：1993-01-19 00:00:00

abstract：:It was previously observed that two steps of the reaction of eukaryotic DNA topoisomerase I (topoisomerization and cleavage) depend upon the conformation of the DNA substrate: in both instances the supercoiled form is a more efficient substrate than the relaxed one. This paper reports the analysis of two other steps o...

journal_title：Biochemistry

authors： Caserta M,Amadei A,Camilloni G,Di Mauro E

更新日期：1990-09-04 00:00:00

abstract：:Recent studies implicate hydrogen sulfide (H2S) oxidation as an important aspect of bacterial antibiotic resistance and sulfide homeostasis. The cst operon of the major human pathogen Staphylococcus aureus is induced by exogenous H2S stress and encodes enzymes involved in sulfide oxidation, including a group I flavopr...

journal_title：Biochemistry

authors： Shen J,Peng H,Zhang Y,Trinidad JC,Giedroc DP

更新日期：2016-11-29 00:00:00

abstract：:The conformational analysis of non-native states of proteins remains one of the most difficult problems in structural biology, because such states are represented by a superimposition of several states that are rapidly interconverting. Hence, model building of the conformational ensemble remains challenging, although ...

journal_title：Biochemistry

authors： Sziegat F,Silvers R,Hähnke M,Jensen MR,Blackledge M,Wirmer-Bartoschek J,Schwalbe H

更新日期：2012-04-24 00:00:00

abstract：:Proteogenomic identification of translated small open reading frames in humans has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many reg...

journal_title：Biochemistry

authors： Na Z,Luo Y,Schofield JA,Smelyansky S,Khitun A,Muthukumar S,Valkov E,Simon MD,Slavoff SA

更新日期：2020-10-27 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, CTP synthetase [EC 6.3.4.2; UTP:ammonia ligase (ADP-forming)] is the product of the URA7 gene. CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme. The purificati...

journal_title：Biochemistry

authors： Yang WL,McDonough VM,Ozier-Kalogeropoulos O,Adeline MT,Flocco MT,Carman GM

更新日期：1994-09-06 00:00:00

abstract：:The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...

journal_title：Biochemistry

authors： Stöckel J,Safar J,Wallace AC,Cohen FE,Prusiner SB

更新日期：1998-05-19 00:00:00

abstract：:Wideline 2H NMR spectroscopy was used to assess the conformational and orientational effects of N-acetylneuraminic acid (NeuAc) (sialic acid) as a component of a particular oligosaccharide chain at a bilayer membrane surface. For this purpose, three glycosphingolipids, sharing a neutral core tetrasaccharide and differ...

journal_title：Biochemistry

authors： Jones DH,Barber KR,Grant CW

更新日期：1996-04-16 00:00:00

abstract：:Studies of the mechanism of protein secretion in a Gram-positive bacterium, Bacillus subtilis, yielded results very similar to those previously obtained with a Gram-negative organism: nascent chains protruding from protoplasts could be labeled extracellularly; the labeled chains could be recovered on polysomes isolate...

journal_title：Biochemistry

authors： Smith WP,Tai PC,Davis BD

更新日期：1979-01-09 00:00:00

abstract：:Two terminally blocked peptides, acetylAETAAAKFLRQHMamide and acetylAETSSSRYLRQHMamide, were obtained by solid-phase synthesis, purified by reversed-phase chromatography, and characterized by fast atom bombardment mass spectrometry. Both peptides were soluble in aqueous solutions and remained monomeric over the concen...

journal_title：Biochemistry

authors： Merutka G,Stellwagen E

更新日期：1989-01-10 00:00:00

abstract：:Typical of many transcriptional regulatory proteins, the lambdoid bacteriophage repressors bind cooperatively to multiple sites on DNA. This cooperative binding is essential for establishment and maintenance of phage lysogeny. In the phage, two repressor homodimers, one bound at each of the adjacent operator sites, in...

journal_title：Biochemistry

authors： Ciubotaru M,Koudelka GB

更新日期：2003-04-15 00:00:00

abstract：:Oxidative damage by transition metals bound to proteins may be an important pathogenic mechanism. Ceruloplasmin (Cp) is a Cu-containing plasma protein thought to be involved in oxidative modification of lipoproteins. We have previously shown that Cp increased cell-mediated low-density lipoprotein (LDL) oxidation by a ...

journal_title：Biochemistry

authors： Mukhopadhyay CK,Fox PL

更新日期：1998-10-06 00:00:00

abstract：:The interaction of the TEM-2 beta-lactamase with 9-deoxyclavulanic acid (3) and with both extensively labeled (2) and specifically labeled (1) clavulanic acid has been studied. The close similarity between 9-doexyclavulanate and clavulanate in kinetics, spectroscopic, and protein chemical terms show that the allyl alc...

journal_title：Biochemistry

authors： Charnas RL,Knowles JR

更新日期：1981-05-26 00:00:00

abstract：:It has previously been shown that mammalian RNA-peptidyl complexes are found in close association with tRNA, but can be separated from the bulk of the tRNA by benzoylated diethylaminoethylcellulose chromatography (Kull, F.J., and Soodak, M. (1971), Biochim. Biophys. Acta 246, l; Gadski, R.A., and Kull, F.J. (1973), Bi...

journal_title：Biochemistry

authors： Fisher PA,Goodman JG,Kull FJ

更新日期：1976-09-07 00:00:00

abstract：:The side chains of R269 and N270 interact with the phosphodianion of dihydroxyacetone phosphate (DHAP) bound to glycerol 3-phosphate dehydrogenase (GPDH). The R269A, N270A, and R269A/N270A mutations of GPDH result in 9.1, 5.6, and 11.5 kcal/mol destabilization, respectively, of the transition state for GPDH-catalyzed ...

journal_title：Biochemistry

authors： Reyes AC,Amyes TL,Richard JP

更新日期：2016-03-15 00:00:00

abstract：:Aminoacyl-tRNA synthetases preserve the fidelity of decoding genetic information by accurately joining amino acids to their cognate transfer RNAs. Here, tRNA discrimination at the level of binding by Escherichia coli histidyl-tRNA synthetase is addressed by filter binding, analytical ultracentrifugation, and iodine fo...

journal_title：Biochemistry

authors： Bovee ML,Yan W,Sproat BS,Francklyn CS

更新日期：1999-10-12 00:00:00

abstract：:The enzyme PvdQ (E.C. 3.5.1.97) from Pseudomonas aeruginosa is an N-terminal nucleophile hydrolase that catalyzes the removal of an N-myristyl substituent from a biosynthetic precursor of the iron-chelating siderophore pyoverdine. Inhibitors of pyoverdine biosynthesis are potential antibiotics since iron is essential ...

journal_title：Biochemistry

authors： Clevenger KD,Wu R,Liu D,Fast W

更新日期：2014-10-28 00:00:00

abstract：:The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be r...

journal_title：Biochemistry

authors： Jackson RC

更新日期：1976-12-14 00:00:00

abstract：:The E-domain of staphylococcal protein A is one of five homologous IgG-binding domains designated E, D, A, B, and C that comprise the extracellular portion of protein A. The E-domain binds tightly to Fc fragments of IgG and binds certain Fv fragments with micromolar affinity. To explore further the structural features...

journal_title：Biochemistry

authors： Starovasnik MA,Skelton NJ,O'Connell MP,Kelley RF,Reilly D,Fairbrother WJ

更新日期：1996-12-03 00:00:00

abstract：:We measure the effects of low concentrations of helix-stabilizing cosolvents, including 2,2,2-trifluoroethanol (TFE), on the thermodynamics and kinetics of folding of the dimeric alpha-helical coiled coil derived from the leucine zipper region of bZIP transcriptional activator GCN4. The change in kinetic behavior upon...

journal_title：Biochemistry

authors： Kentsis A,Sosnick TR

更新日期：1998-10-13 00:00:00

abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...

journal_title：Biochemistry

authors： Kasson PM,Rabinowitz JD,Schmitt L,Davis MM,McConnell HM

更新日期：2000-02-08 00:00:00

abstract：:The cytochrome bc(1) complex from Paracoccus denitrificans and soluble fragments of its cytochrome c(1) and Rieske ISP subunits are characterized by a combined approach of protein electrochemistry and FTIR difference spectroscopy. The FTIR difference spectra provide information about alterations in the protein upon re...

journal_title：Biochemistry

authors： Ritter M,Anderka O,Ludwig B,Mäntele W,Hellwig P

更新日期：2003-10-28 00:00:00

abstract：:Rett syndrome causing missense mutations in the methyl-CpG-binding domain (MBD) of methyl CpG-binding protein 2 (MeCP2) were investigated both in silico and in vitro to reveal their effect on protein stability. It is demonstrated that the vast majority of frequently occurring mutations in the human population indeed a...

journal_title：Biochemistry

authors： Kucukkal TG,Yang Y,Uvarov O,Cao W,Alexov E

更新日期：2015-10-20 00:00:00