Abstract：
:Studies of the mechanism of protein secretion in a Gram-positive bacterium, Bacillus subtilis, yielded results very similar to those previously obtained with a Gram-negative organism: nascent chains protruding from protoplasts could be labeled extracellularly; the labeled chains could be recovered on polysomes isolated from the membrane--polysome fraction; they could be released by puromycin, low Mg2+, or chain completion; the completed chains include a known secreted protein (alpha-amylase); and their ribosomes appear to be attached to membrane solely by their nascent chains. The reagent used for extracellular labeling, [1252]diazoiodosulfanilic acid, yielded severalfold more specific labeling of the nascent chains (7--10% of the total cellular labeling and one-fourth to one-third of that of the membrane--polysome fraction) than was obtained earlier with another nonpenetrating reagent.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Smith WP,Tai PC,Davis BDdoi
10.1021/bi00568a030subject
Has Abstractpub_date
1979-01-09 00:00:00pages
198-202issue
1eissn
0006-2960issn
1520-4995journal_volume
18pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Human hemoglobin produced in the Escherichia coli coexpression system of Hernan et al. [(1992) Biochemistry 31, 8619-8628] has been transformed into a functionally homogeneous protein whose properties closely approximate those of normal hemoglobin A. Both of the alpha and beta chains of this hemoglobin contain a valin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981986g
更新日期：1999-01-19 00:00:00
abstract：:The aminolysis of the depsipeptide m-[[(phenylacetyl)glycyl]oxy]benzoic acid (1) by D-phenylalanine, catalyzed by the beta-lactamase of Enterobacter cloacae P99, is inhibited by the product of the reaction, (phenylacetyl)glycyl-D-phenylalanine (2), by the peptide analogue of 1, m-[(phenylacetyl)-glycinamido]benzoic ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00443a015
更新日期：1989-08-22 00:00:00
abstract：:The VWF A1 domain seems to possess two heparin binding regions (residues 565-587 and 633-648) displaying positively charged amino acids, but the overall polyanion-A1 domain interaction scheme remains essentially elusive. To probe this molecular reaction as well as the role of electrostatic forces in VWF-heparin intera...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020044f
更新日期：2002-05-28 00:00:00
abstract：:Transcripts of Saccharomyces cerevisiae nuclear tRNA genes are normally terminated within a few nucleotides of the tRNA coding region, in contrast to mitochondrially encoded tRNAs, which are contained within polycistronic transcripts and thus require 3'-processing by mitochondrial endonucleases. We show that 3'-proces...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00159a024
更新日期：1992-11-10 00:00:00
abstract：:Kinesin-5 proteins are essential for formation of a bipolar mitotic spindle in most and, perhaps all, eukaryotic cells. Several Kinesin-5 proteins, notably the human version, HsEg5, are targets of a constantly expanding group of small-molecule inhibitors, which hold promise both as tools for probing mechanochemical tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801291q
更新日期：2009-03-03 00:00:00
abstract：:The thermodynamics of the native<-->A state and native<-->unfolded transitions for ubiquitin have been characterized in detail using the denaturants methanol and guanidinium chloride (Gdn.HCl) both separately and in combination. Gdn.HCl destabilizes the partially folded alcohol-induced A state such that the effects of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010767j
更新日期：2001-08-28 00:00:00
abstract：:Bacteriophage T7 codes for a single-stranded DNA binding protein. This protein is the product of gene 2.5 and has been found previously to stimulate specifically the activity of the phage-coded DNA polymerase. We report here that the T7 DNA binding protein also stimulates the activity of the phage-coded exonuclease. T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00266a042
更新日期：1982-11-09 00:00:00
abstract：:The structural determinants of substrate specificity of human liver cytochrome P450 2C8 (CYP2C8) were investigated using site-directed mutants chosen on the basis of a preliminary substrate pharmacophore and a three-dimensional (3D) model. Analysis of the structural features common to CYP2C8 substrates exhibiting a mi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0489309
更新日期：2004-12-14 00:00:00
abstract：:Glycerol stabilizes the triple-helical structure of solubilized calf skin collagen. The equilibrium melting temperature of the protein increased linearly from 38.0 degrees C in AS buffer (0.01 M NaOAc and 0.02 M NaCl, pH 4.0) to 43.0 degrees C in AS and 6 M glycerol buffer. To understand the thermodynamic basis of thi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00353a004
更新日期：1986-03-11 00:00:00
abstract：:Firefly luciferase is 80-90% inactivated within 3 h upon incubation with the adenine nucleotide analogue p-fluorosulfonylbenzoyl-5'-adenosine (FSBA). Although 4 mol of 14C-FSBA/mol of enzyme is irreversibly bound during inactivation, only 1 mol of 14C-FSBA appears to be specifically directed to an adenine nucleotide b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00508a031
更新日期：1981-03-03 00:00:00
abstract：:The folding behavior of bovine growth hormone (bGH) is examined by chemical and pH denaturation using several spectroscopic probes of protein secondary and tertiary structure. Partially denaturing concentrations of urea eliminate the native-state quenching of intrinsic tryptophan fluorescence, from the single protein ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00457a022
更新日期：1990-02-06 00:00:00
abstract：:Plexins are transmembrane proteins that serve as guidance receptors during angiogenesis, lymphangiogenesis, neuronal development, and zebrafish fin regeneration, with a putative role in cancer metastasis. Receptor dimerization or clustering, induced by extracellular ligand binding but modulated in part by the plexin t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00517
更新日期：2016-09-06 00:00:00
abstract：:The mature form of subtilisin is an unusual example of a monomeric protein with a high kinetic barrier to folding and unfolding. Using site-directed mutagenesis of subtilisin BPN', we are attempting to determine the physical and energetic nature of the kinetic barrier. The high-affinity calcium-binding site A has been...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00090a012
更新日期：1993-10-05 00:00:00
abstract：:Monomeric sarcosine oxidase is a flavoenzyme that catalyzes the oxidation of the methyl group in sarcosine (N-methylglycine). Rapid reaction kinetic studies under anaerobic conditions at pH 8.0 show that the enzyme forms a charge transfer Michaelis complex with sarcosine (E-FAD(ox).sarcosine) that exhibits an intense ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0600852
更新日期：2006-05-16 00:00:00
abstract：:The iron-chelating peptide vibriobactin of the pathogenic Vibrio cholerae is assembled by a four-subunit nonribosomal peptide synthetase complex, VibE, VibB, VibH, and VibF, using 2,3-dihydroxybenzoate and L-threonine as precursors to two 2,3-dihydroxyphenyl- (DHP-) methyloxazolinyl groups in amide linkage on a norspe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010937s
更新日期：2001-09-04 00:00:00
abstract：:High-resolution, solid-state 15N NMR has been used to study the chemical shift anisotropies of the Schiff bases in bacteriorhodopsin (bR) and in an extensive series of model compounds. Using slow-spinning techniques, we are able to obtain sufficient rotational sideband intensity to determine the full 15N chemical shif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00434a033
更新日期：1989-04-18 00:00:00
abstract：:Human S-adenosylmethionine decarboxylase (AdoMetDC) was expressed in high yield in Escherichia coli using the pIN-III(lppP-5) expression vector and purified to apparent homogeneity using affinity chromatography on methylglyoxal bis(guanylhydrazone)-Sepharose. The inactivation of the purified enzyme by 5'-deoxy-5'-[(3-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00144a027
更新日期：1992-07-28 00:00:00
abstract：:We have developed an assay to continuously monitor the branched amino acid preferring peptidase (BrAAP) activity of the proteasome. This assay is based on the hydrolysis of the fluorogenic peptide, Abz-Gly-Pro-Ala-Leu-Ala-Nba (Abz is 2-aminobenzoyl and Nba is 4-nitrobenzylamide) which is cleaved exclusively at the Leu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980097q
更新日期：1998-05-26 00:00:00
abstract：:Aspartate-beta-semialdehyde dehydrogenase (ASADH) lies at the first branch point in the biosynthetic pathway through which bacteria, fungi, and the higher plants synthesize amino acids, including lysine and methionine and the cell wall component diaminopimelate from aspartate. Blocks in this biosynthetic pathway, whic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015713o
更新日期：2001-12-04 00:00:00
abstract：:The membrane-bound beta-subunit of oxaloacetate decarboxylase from Klebsiella pneumoniae catalyzes the decarboxylation of carboxybiotin, which is coupled to Na(+) translocation and consumes a periplasmically derived proton. Upon site-directed mutagenesis of 20 polar and/or conserved residues within putative membrane-i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992817e
更新日期：2000-04-18 00:00:00
abstract：:Structure-function studies of antibody-antigen systems include the identification of amino acid residues in the antigen that interact with an antibody and elucidation of their individual contributions to binding affinity. We used fluorescence correlation spectroscopy (FCS) and alanine-scanning mutagenesis to character...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0607047
更新日期：2006-11-28 00:00:00
abstract：:Multiple forms of the acute-phase serum protein SAA were isolated from the lipoprotein fraction of plasma from a single individual. These protein forms were purified by size-exclusion, ion-exchange, and reverse-phase high-pressure liquid chromatography, and then the tryptic peptides were subjected to amino acid sequen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00405a044
更新日期：1988-03-08 00:00:00
abstract：:An organic solvent soluble polypeptide has been isolated from photoreceptor complexes and chromatophores of Rhodospirillum rubrum. After extraction of the protein from lyophilized samples with 1:1 chloroform-methanol, it was purified by column chromatography. Its isoelectric point determined by isoelectric focusing wa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00624a011
更新日期：1977-03-08 00:00:00
abstract：:A 16-base pair oligo(purine)-oligo(pyrimidine) sequence present in the coding region of two HIV 1 proviral genes (pol and nef) was chosen as a target for triplex-forming oligonucleotides in in vitro transcription assays. Inhibition of transcription elongation was observed with triplex-forming oligonucleotide-acridine ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952993x
更新日期：1996-08-13 00:00:00
abstract：:The hydrogen exchange behavior of a four-helix bundle protein in low concentrations of denaturant reveals some partially unfolded forms that are significantly more stable than the fully unfolded state. Kinetic folding of the protein, however, is apparently two-state in the absence of the accumulation of early folding ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025872n
更新日期：2002-06-25 00:00:00
abstract：:1H NMR and ligand-binding data were used to assess the role of residue Arg(E10) in ligand stabilization of several site-directed mutants, all carrying the His(E7) to Val substitution, obtained using a synthetic sperm whale myoglobin gene. Arg(E10) was previously found to form a hydrogen bond with the ligand in fluoro-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00074a015
更新日期：1993-06-15 00:00:00
abstract：:Decarboxylation reactions from comparable thiamin diphosphate- and thiamin-derived adducts of p-(halomethyl)benzoylformic acids in enzymic and non-enzymic reactions, respectively, reveal critical distinctions in otherwise similar Breslow intermediates. The ratio of protonation to chloride elimination from the Breslow ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00298
更新日期：2019-08-27 00:00:00
abstract：:The flap endonuclease, FEN1, plays a critical role in DNA replication and repair. Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic activity. On primer-template substrates containing an unannealed 5'-tail, or flap structure, FEN1 employs a unique mechanism to cleave at the poi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991321u
更新日期：1999-10-05 00:00:00
abstract：:Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700726a
更新日期：2007-10-23 00:00:00
abstract：:The synthesis of 5-(2-oxalylethyl)-NADH, a reduced nicotinamide adenine dinucleotide (NADH) derivate with pyruvate covalently attached to the 5 position of the dihydronicotinamide ring over an additional methylene group has been described previously (Trommer, W.E., Blume, H., and Kapmeyer, H. (1976) Justus Liebigs Ann...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00668a012
更新日期：1976-11-16 00:00:00
