Abstract:
:Bacteriophage T7 codes for a single-stranded DNA binding protein. This protein is the product of gene 2.5 and has been found previously to stimulate specifically the activity of the phage-coded DNA polymerase. We report here that the T7 DNA binding protein also stimulates the activity of the phage-coded exonuclease. The gene 6 exonuclease is a double-stranded DNA specific 5'-exonuclease that has been implicated in destruction of bacterial DNA, removal of RNA primers during DNA replication, genetic recombination, and DNA maturation. The enzyme is markedly inhibited by physiological concentrations of NaCl. This inhibition, which is due to a marked reduction in the Vmax of the enzyme, can be largely overcome by the phage-coded DNA binding protein. This stimulation is specific since the Escherichia coli DNA binding protein is without effect. The stimulation by the binding protein is apparently not due to its coating of the 3' single-stranded tails generated during the digestion. Kinetic studies show that the stimulation is due to a combined effect on both the Km and Vmax of the exonuclease. These studies are consistent with a loose binding of the binding protein to either the DNA or the exonuclease.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Roberts L,Sadowski P,Wong JTdoi
10.1021/bi00266a042subject
Has Abstractpub_date
1982-11-09 00:00:00pages
6000-5issue
23eissn
0006-2960issn
1520-4995journal_volume
21pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The crystal structure of the complex between the quinoprotein methylamine dehydrogenase (MADH) and the type I blue copper protein amicyanin, both from Paracoccus denitrificans, has been determined at 2.5-A resolution using molecular replacement. The search model was MADH from Thiobacillus versutus. The amicyanin could...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00136a006
更新日期:1992-06-02 00:00:00
abstract::Chorismate-utilizing enzymes catalyze diverse reactions, providing critical physiological functions unique to plants, bacteria, fungi, and some parasites. Their absence in animals makes them excellent targets for antimicrobials and herbicides. 4-Amino-4-deoxychorismate synthase (ADCS) catalyzes the first step in folat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052216p
更新日期:2006-04-18 00:00:00
abstract::In order to evaluate the contributions that histidine residues might play both in the catalytic activities of endonuclease V and in binding to nontarget DNA, the technique of oligonucleotide site directed mutagenesis was used to create mutations at each of the four histidine residues in the endonuclease V gene. Althou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00246a025
更新日期:1991-08-13 00:00:00
abstract::In recent years, there has been dramatic growth in the study of RNA. RNA has gone from being known as an intermediate in the central dogma of molecular biology to a molecule with a large diversity of structure and function that is involved in all aspects of biology. As new functions are rapidly discovered, it has beco...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01141
更新日期:2018-08-07 00:00:00
abstract::Many drugs are oxidized by membrane protein cytochrome P450 (CYP) enzymes during their metabolism process. CYPs are located mainly in endoplasmic reticulum (ER) membranes. Recent studies have suggested that CYP substrate drugs first bind the lipid bilayers of ER membranes and then the drugs reach the active site of CY...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00286
更新日期:2016-07-19 00:00:00
abstract::The essential prokaryotic cell division protein FtsZ is a tubulin homologue that forms a ring at the division site. FtsZ forms polymers in a GTP-dependent manner. Recent biochemical evidence has shown that FtsZ forms multimeric structures in vitro and in vivo and functions as a self-activating GTPase. Structural analy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011370i
更新日期:2002-01-15 00:00:00
abstract::According to the X-ray crystallographic results from human deoxyhemoglobin, beta 99Asp at the alpha 1 Beta 2 interface forms hydrogen bonds with alpha 42Tyr and alpha 97Asn. To clarify the structural and functional roles of the hydrogen bond between alpha 97Asn and beta 99Asp, we have engineered a recombinant hemoglob...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952518z
更新日期:1996-05-28 00:00:00
abstract::Salmonella typhimurium L-histidinol dehydrogenase (EC 1.1.1.23), a four-electron dehydrogenase, was inactivated by an active-site-directed modification reagent, 7-chloro-4-nitro-2,1,3-benzoxadiazole (NBD-Cl). The inactivation followed pseudo-first-order kinetics and was prevented by low concentrations of the substrate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00365a009
更新日期:1986-08-26 00:00:00
abstract::The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00462a002
更新日期:1990-03-13 00:00:00
abstract::We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00402a012
更新日期:1988-01-26 00:00:00
abstract::The photosynthetic antenna of Chloroflexus aurantiacus includes bacteriochlorophyll (BChl) C740 and BChl a792, both of which occur in chlorosomes, and B808-866 (containing BChl a808 and BChl a866), which is membrane-located (subscripts refer to near-infrared absorption maxima in vivo). BChl a792 is thought to mediate ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00400a024
更新日期:1987-12-29 00:00:00
abstract::Cytochrome c oxidase contains four redox-active metal centers: two heme irons, cytochromes a and a3, and two copper ions, CuA and CuB. Due to the paucity of spectroscopic signatures for both copper sites in cytochrome c oxidase, the ligands and structures for these sites have remained ambiguous. The specific depletion...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00382a005
更新日期:1987-04-21 00:00:00
abstract::Regulation of Saccharomyces cerevisiae membrane-associated phosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by phospholipids was examined using purified enzyme and Triton X-100/phospholipid-mixed micelles. Anionic phospholipids activated phosphatidate phosphatase activity whereas zw...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952808f
更新日期:1996-03-26 00:00:00
abstract::The Staphylococcus aureus sortase transpeptidase SrtA isoform is responsible for the covalent attachment of virulence and colonization-associated proteins to the bacterial peptidoglycan. SrtA utilizes two substrates, undecaprenol-pyrophosphoryl-MurNAc(GlcNAc)-Ala-D-isoGlu-Lys(epsilon-Gly(5))-D-Ala-D-Ala (branched Lipi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035920j
更新日期:2004-02-17 00:00:00
abstract::The aggregation of the microtubule-associated protein, tau, into amyloid fibrils is a hallmark of neurodegenerative diseases such as the tauopathies and Alzheimer's disease. Since monomeric tau is an intrinsically disordered protein and the polymeric fibrils possess an ordered cross-β core, the aggregation process is ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500528x
更新日期:2014-10-21 00:00:00
abstract::L6 is a monoclonal antitumor antibody which recognizes an epitope located in a 42-residue extracellular domain of a tumor-associated approximately 22 kDa glycoprotein antigen. The L6 mAb localizes to solid tumors in vivo and triggers complement activation and antibody-dependent cellular cytotoxicity. It has been the s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00252a005
更新日期:1994-12-06 00:00:00
abstract::S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900590m
更新日期:2009-07-14 00:00:00
abstract::Human secretory group IIa phospholipase A2 (hIIa-PLA2) contains a large number of prominent cationic patches on its molecular surface and has exceptionally high affinity for anionic surfaces, including anionic membranes. To identify the cationic amino acid residues that support binding of hIIa-PLA2 to anionic membrane...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971200z
更新日期:1997-11-25 00:00:00
abstract::The membrane interaction properties of two single-residue variants, R6W and L5S, of the 17-amino acid neuropeptide dynorphin A (DynA) were studied by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy. Corresponding gene mutations have recently been discovered in humans and causatively linked to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4004205
更新日期:2013-06-18 00:00:00
abstract::The complete amino acid sequence of human liver NADPH-cytochrome P-450 reductase has been determined by microsequence analysis and mass spectrometry. The total sequence consists of 676 amino acids initiated by an amino-terminal acetyl group. There is no evidence for posttranslational modifications, including Asn-linke...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00447a054
更新日期:1989-10-17 00:00:00
abstract::The rate of deuterium exchange of a purine 8CH group in DNA is highly sensitive to both macromolecular secondary structure and intermolecular interactions which restrict solvent access to the major groove [Lamba, O.P., Becka, R., & Thomas, G.J., Jr. (1990) Biopolymers 29, 1465-1477]. We have exploited the sensitivity ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00127a012
更新日期:1992-03-31 00:00:00
abstract::The existence of two conformational states of concanavalin A (Con A) with different metal ion binding properties has been recently demonstrated (Brown, R. D., Brewer, C. F., & Koenig, S. H. (1977) Biochemistry 16, 3883). Introduction of Mn2+ to the S1 site and Ca2+ to the S2 site of apo-Con A was shown to induce a con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00613a022
更新日期:1978-10-03 00:00:00
abstract::Venomous organisms have evolved a variety of structurally diverse peptide neurotoxins that target ion channels. Despite the lack of any obvious structural homology, unrelated toxins that interact with voltage-activated K(+) channels share a dyad motif composed of a lysine and a hydrophobic amino acid residue, usually ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0495681
更新日期:2004-07-13 00:00:00
abstract::The enzymes in the alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily represent the largest class of non-heme iron oxidases and have important medical, ecological, and biotechnological roles. One such enzyme, taurine/alpha-ketoglutarate dioxygenase (TauD), catalyzes the conversion of 2-aminoethanesulfonat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0341096
更新日期:2003-05-20 00:00:00
abstract::Exogenous mannosylphosphoryldolichol (Man-P-Dol) has previously been shown to stimulate UDP-GlcNAc:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GPT1), the enzyme catalyzing the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol (GlcNAc-P-P-Dol). To define the structural specificity of the ma...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00200a036
更新日期:1994-08-30 00:00:00
abstract::Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00552a023
更新日期:1980-05-27 00:00:00
abstract::The ferlin family proteins have emerged as multi-C2 domain regulators of calcium-triggered membrane fusion and fission events. While initially determined to share many of the features of members of the synaptotagmin family of calcium sensors, ferlins in more recent studies have been found to interact directly with non...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00928
更新日期:2017-12-12 00:00:00
abstract::Ceruloplasmin is unique among the multicopper oxidases in that in addition to the usual copper stoichiometry of one Type 1 copper site and a Type 2/Type 3 trinuclear copper cluster, it contains two other Type 1 sites. This assignment of copper sites, based on copper quantitation, sequence alignment, and crystallograph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980434v
更新日期:1998-06-30 00:00:00
abstract::The relative quantitative importance of the factors that determine the fidelity of translation is largely unknown, which makes predicting the extent to which the degeneracy of the genetic code can be broken challenging. Our strategy of using orthogonal tRNA/aminoacyl tRNA synthetase pairs to precisely direct the incor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00177
更新日期:2018-05-15 00:00:00
abstract::Bacterial bioluminescence is a phenomenon resulting from the reaction of a two-component FMN-dependent aldehyde monooxygenase system, which comprises a bacterial luciferase and a flavin reductase. Bacterial luciferase (LuxAB) is one of the most extensively investigated two-component monooxygenases, while its reductase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1009985
更新日期:2010-11-02 00:00:00