Engineering of the nonspecific phospholipase C from Bacillus cereus: replacement of glutamic acid-4 by alanine results in loss of interfacial catalysis and enhanced phosphomonoesterase activity.

abstract：:The binding of ethidium bromide to the self-complementary deoxytetranucleotides containing guanine and cytosine bases has been studied by circular dichroism, visible absorption, and fluorescence spectroscopies. The circular dichroism spectrum of each of the four deoxytetranucleotides was measured and compared to the s...

journal_title：Biochemistry

authors： Kastrup RV,Young MA,Krugh TR

更新日期：1978-11-14 00:00:00

abstract：:Aminoglycoside 3'-phosphotransferases [APH(3')s] are the most common cause of bacterial high-level resistance to aminoglycoside antibiotics in clinical isolates. A one-step affinity chromatography was used to purify APH(3') type Ia. The kinetic parameters for turnover of seven aminoglycosides and the corresponding min...

journal_title：Biochemistry

authors： Siregar JJ,Miroshnikov K,Mobashery S

更新日期：1995-10-03 00:00:00

abstract：:The ionic and nucleotide requirements for the in vitro polymerization of microtubules from purified brain tubulin have been characterized by viscometry. Protein was purified by successive cycles of a temperature dependent assembly-diassembly scheme. Maximal polymerization occurred at a concentration of 0.1 M Pipes (pi...

journal_title：Biochemistry

authors： Olmsted JB,Borisy GG

更新日期：1975-07-01 00:00:00

abstract：:Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...

journal_title：Biochemistry

authors： Kuo W,Herrick DZ,Cafiso DS

更新日期：2011-04-05 00:00:00

abstract：:The active site topography of rabbit CYP4B1 has been studied relative to CYP2B1 and CYP102 using a variety of aromatic probe substrates. Oxidation of the prochiral substrate cumene by CYP4B1, but not CYP2B1 or CYP102, resulted in the formation of the thermodynamically disfavored omega-hydroxy metabolite, 2-phenyl-1-pr...

journal_title：Biochemistry

authors： Henne KR,Fisher MB,Iyer KR,Lang DH,Trager WF,Rettie AE

更新日期：2001-07-24 00:00:00

abstract：:Phase partition techniques have been used to measure the binding of the antitumor drugs adriamycin (NSC-123127) and daunorubicin (NSC-82151) to various DNAs. These methods provide reliable equilibrium binding data at the low levels of drug binding that may be expected in vivo. Both adriamycin and daunorubicin exhibit ...

journal_title：Biochemistry

authors： Graves DE,Krugh TR

更新日期：1983-08-02 00:00:00

abstract：:In this work we modeled the circular dichroism (CD) spectrum of LHCII, the main light harvesting antenna of photosystem II of higher plants. Excitonic calculations are performed for a monomeric subunit, taken from the crystal structure of trimeric LHCII from spinach [Liu, Z. F., Yan, H. C., Wang, K. B., Kuang, T. Y., ...

journal_title：Biochemistry

authors： Georgakopoulou S,van der Zwan G,Bassi R,van Grondelle R,van Amerongen H,Croce R

更新日期：2007-04-24 00:00:00

abstract：:MutY protein, a DNA glycosylase found in Escherichia coli, recognizes dA:dG, dA:8-oxodG, and dA:dC mismatches in duplex DNA, excising the adenine moiety. We have investigated the mechanism of action of MutY, addressing several points of disagreement raised by previous studies of this enzyme. MutY forms a covalent inte...

journal_title：Biochemistry

authors： Zharkov DO,Grollman AP

更新日期：1998-09-08 00:00:00

abstract：:Epoxides are cancer-causing agents chemically analogous to the nitrogen mustards, a family of powerful antitumor drugs. We found that the DNA interstrand cross-linking sequence preference of diepoxybutane is the same as that of the mustard mechlorethamine: 5'-GNC. Therefore, the genomic site of cross-linking alone can...

journal_title：Biochemistry

authors： Millard JT,White MM

更新日期：1993-03-02 00:00:00

abstract：:The highly processive motor, myosin V, has an extremely long neck containing six calmodulin-binding IQ motifs that allows it to take multiple 36 nm steps corresponding to the pseudo-repeat of actin. To further investigate how myosin V moves processively on actin filaments, we altered the length of the neck by adding o...

journal_title：Biochemistry

authors： Sakamoto T,Yildez A,Selvin PR,Sellers JR

更新日期：2005-12-13 00:00:00

abstract：:The structure and backbone dynamics of an extended second transmembrane segment (TM2e) of the human neuronal glycine receptor alpha(1) subunit in sodium dodecyl sulfate micelles were studied by (1)H and (15)N solution-state NMR. The 28-amino acid segment contained the consensus TM2 domain plus part of the linker betwe...

journal_title：Biochemistry

authors： Yushmanov VE,Mandal PK,Liu Z,Tang P,Xu Y

更新日期：2003-04-08 00:00:00

abstract：:The light-driven enzyme NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), a key regulatory step in the chlorophyll biosynthesis pathway. As POR is light activated, it allows intermediates in the reaction pathway to be observed by initiat...

journal_title：Biochemistry

authors： Heyes DJ,Ruban AV,Hunter CN

更新日期：2003-01-21 00:00:00

abstract：:Oligopeptides and dipeptides are transported into Saccharomyces cerevisiae by a carrier-mediated system. In the dark, leucyl-p-nitroanilide (Leu-p-NA) and leucyl-leucyl-4-azido-2-nitrophenylalanine [Leu-Leu-Phe-(4N3,2NO2)] are competitive inhibitors of peptide transport by S. cerevisiae cells. The photolysis of yeast ...

journal_title：Biochemistry

authors： Becker JM,Dunsmore KP,Steinfeld AS,Naider F

更新日期：1982-11-09 00:00:00

abstract：:Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure through the application of multi-wavelength anomalous diffraction techniques [Yang et al. (1990) Science (Washington, D.C.) 249, 1398-1405]. The availability of SeMet...

journal_title：Biochemistry

authors： Boles JO,Cisneros RJ,Weir MS,Odom JD,Villafranca JE,Dunlap RB

更新日期：1991-11-19 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:Cdx and Hox gene families encode homeodomain-containing transcription factors involved in anterior-posterior vertebral patterning. Although Cdx proteins are direct transcriptional regulators of Hox gene expression, both Hox and Cdx proteins are known to interact with other homeodomain transcription factors, leading us...

journal_title：Biochemistry

authors： Lafontaine CA,Grainger S,Hess BL,Béland M,Lohnes D

更新日期：2012-12-04 00:00:00

abstract：:3-Deoxy-d-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) to form KDO8P, a key precursor in the biosynthesis of the endotoxin of Gram-negative bacteria. Earlier studies have established that the condensation occurs with a syn addit...

journal_title：Biochemistry

authors： Tao P,Gatti DL,Schlegel HB

更新日期：2009-12-15 00:00:00

abstract：:Previous studies have demonstrated that lung-specific proteins are associated with surfactant lipids, particularly the highly surface active subfraction known as tubular myelin. We have isolated a surfactant-associated protein complex with molecular weight components of 36 000, 32 000, and 28 000 and reassembled it wi...

journal_title：Biochemistry

authors： Hawgood S,Benson BJ,Hamilton RL Jr

更新日期：1985-01-01 00:00:00

abstract：:GGAs are a family of vesicle-coating regulatory proteins that function in intracellular protein transport. A GGA molecule contains four domains, each mediating interaction with other proteins in carrying out intracellular transport. The GAT domain of GGAs has been identified as the structural entity that binds membran...

journal_title：Biochemistry

authors： Zhu G,Zhai P,He X,Terzyan S,Zhang R,Joachimiak A,Tang J,Zhang XC

更新日期：2003-06-03 00:00:00

abstract：:Two dioxygenases (ARD and ARD') were cloned from Klebsiella pneumoniae that catalyze different oxidative decomposition reactions of an advanced aci-reductone intermediate, CH(3)SCH(2)CH(2)COCH(OH)=CH(OH) (I), in the methionine salvage pathway. The two enzymes are remarkable in that they have the same polypeptide seque...

journal_title：Biochemistry

authors： Dai Y,Pochapsky TC,Abeles RH

更新日期：2001-05-29 00:00:00

abstract：:Diphtheria toxin (DT) has been studied as a model for understanding active-site structure and function in the ADP-ribosyltransferases. Earlier evidence suggested that histidine-21 of DT is important for the ADP-ribosylation of eukaryotic elongation factor 2 (EF-2). We have generated substitutions of this residue by ca...

journal_title：Biochemistry

authors： Blanke SR,Huang K,Wilson BA,Papini E,Covacci A,Collier RJ

更新日期：1994-05-03 00:00:00

abstract：:Kinetic study of a series of compounds containing the thrombin-directed peptide D-Phe-ProboroArg-OH had indicated that the structure of the N-terminal blocking group may be correlated with binding [Kettner, C., Mersinger, L., & Knabb, R. (1990) J. Biol. Chem. 265, 18289-18297]. In order to further study this phenomeno...

journal_title：Biochemistry

authors： Nienaber VL,Mersinger LJ,Kettner CA

更新日期：1996-07-30 00:00:00

abstract：:Choline oxidase catalyzes the oxidation of choline to glycine betaine through a two-step, four-electron reaction with betaine aldehyde as an intermediate. Oxygen is the final electron acceptor. Alcohol oxidation is initiated by the removal of the substrate hydroxyl proton by an unknown active site residue with a pKa v...

journal_title：Biochemistry

authors： Smitherman C,Rungsrisuriyachai K,Germann MW,Gadda G

更新日期：2015-01-20 00:00:00

abstract：:Displacement of the fluorescent substrate analogue methylanthraniloyl ADP (mant-ADP) from kinesin by excess ATP results in a biphasic fluorescent transient. The pH and microtubule dependence of the rates and amplitudes indicates that the two phases are produced by release of bound mant-ADP, with an excess of the 3'-is...

journal_title：Biochemistry

authors： Cheng JQ,Jiang W,Hackney DD

更新日期：1998-04-14 00:00:00

abstract：:The sialic acid N-acetylneuraminic acid (NANA) has a key role in the pathogenesis of a select number of neuroinvasive bacteria such as Neisseria meningitidis. These pathogens coat themselves with polysialic acids, mimicking the exterior surface of mammalian cells and consequentially concealing the bacteria from the ho...

journal_title：Biochemistry

authors： Joseph DD,Jiao W,Parker EJ

更新日期：2013-04-16 00:00:00

abstract：:Clip domains are structural modules found in arthropod serine proteinases and some proteolytically inactive homologues, which mediate extracellular signaling pathways of development and immunity. While little is known about their structures or functions, clip domains are proposed to be sites for interactions of protei...

journal_title：Biochemistry

authors： Huang R,Lu Z,Dai H,Velde DV,Prakash O,Jiang H

更新日期：2007-10-16 00:00:00

abstract：:The aggregation of the microtubule-associated protein, tau, into amyloid fibrils is a hallmark of neurodegenerative diseases such as the tauopathies and Alzheimer's disease. Since monomeric tau is an intrinsically disordered protein and the polymeric fibrils possess an ordered cross-β core, the aggregation process is ...

journal_title：Biochemistry

authors： Ramachandran G,Milán-Garcés EA,Udgaonkar JB,Puranik M

更新日期：2014-10-21 00:00:00

abstract：:The conformational constraints for apoA-I bound to recombinant phospholipid complexes (rHDL) were attained from a combination of chemical cross-linking and mass spectrometry. Molecular distances were then used to refine models of lipid-bound apoA-I on both 80 and 96 A diameter rHDL particles. To obtain molecular const...

journal_title：Biochemistry

authors： Bhat S,Sorci-Thomas MG,Tuladhar R,Samuel MP,Thomas MJ

更新日期：2007-07-03 00:00:00

abstract：:The repeating units of mouse, rat, and alpha-monkey satellites have been cloned. All three show properties that are characteristic of curved DNA: (i) their migration in polyacrylamide gels is slower than predicted from their sequences, and (ii) they appear as curved molecules when visualized by electron microscopy. Al...

journal_title：Biochemistry

authors： Martínez-Balbás A,Rodríguez-Campos A,García-Ramírez M,Sainz J,Carrera P,Aymamí J,Azorín F

更新日期：1990-03-06 00:00:00

abstract：:Inherently curved DNA segments, associated with short runs of adenines, have been identified in many gene regulatory regions, yet their physiological significance remains unknown. The observations reported in this study indicate that intrinsically bent nucleic acid fragments are characterized by substantially attenuat...

journal_title：Biochemistry

authors： Shatzky-Schwartz M,Hiller Y,Reich Z,Ghirlando R,Weinberger S,Minsky A

更新日期：1992-03-03 00:00:00