Abstract:
:The sialic acid N-acetylneuraminic acid (NANA) has a key role in the pathogenesis of a select number of neuroinvasive bacteria such as Neisseria meningitidis. These pathogens coat themselves with polysialic acids, mimicking the exterior surface of mammalian cells and consequentially concealing the bacteria from the host's immune system. NANA is synthesized in bacteria by the homodimeric enzyme NANA synthase (NANAS), which catalyzes a condensation reaction between phosphoenolpyruvate (PEP) and N-acetylmannosamine (ManNAc). NANAS is closely related to the α-keto acid synthases 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase and 3-deoxy-d-manno-octulosonate 8-phosphate synthase. NANAS differs from these enzymes in that it contains an antifreeze protein like (AFPL) domain, which extends from the C-terminal of the (β/α)8 barrel containing the active site and contributes a highly conserved arginine (Arg314) into the active site of the opposing monomer chain. We have investigated the role of Arg314 in NmeNANAS through mutagenesis and a combination of kinetic and structural analyses. Using isothermal titration calorimetry and molecular modeling, we have shown that Arg314 is required for the catalytic function of NANAS and that the delocalized positively charged guanidinium functionality of this residue provides steering of the sugar substrate ManNAc for suitable placement in the active site and thus reaction with PEP.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Joseph DD,Jiao W,Parker EJdoi
10.1021/bi400062csubject
Has Abstractpub_date
2013-04-16 00:00:00pages
2609-19issue
15eissn
0006-2960issn
1520-4995journal_volume
52pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Electron spin polarized electron paramagentic resonance (ESP EPR) spectra were obtained with deuterated iron-removed photosynthetic bacterial reaction centers (RCs) to specifically investigate the effect of the rate of primary charge separation, metal-site occupancy, and H-subunit content on the observed P865+QA- char...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9630319
更新日期:1997-07-15 00:00:00
abstract::The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01066
更新日期:2019-02-12 00:00:00
abstract::Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(II) dependent agmatinase (SpeB). This is the first report of an iron-dependent agmatinase. We demonstrate that aerobically isolated recombinant enzyme contains two disulfide bonds and only a trace amount of any metal and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300039f
更新日期:2012-04-10 00:00:00
abstract::Annexin A1 is a multifunctional, calcium-dependent phospholipid binding protein involved in a host of processes including inflammation, regulation of neuroendocrine signaling, apoptosis, and membrane trafficking. Binding of annexin A1 to glycans has been implicated in cell attachment and modulation of annexin A1 funct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101121a
更新日期:2011-04-05 00:00:00
abstract::Alpha2u-globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. We have measured the rate of transcription of the alpha2u-globulin gene using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polym...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00608a023
更新日期:1978-07-25 00:00:00
abstract::Several diseases, including Alzheimer's disease, Parkinson's disease, and Huntington's disease (HD), are associated with specific proteins aggregating and depositing within tissues and/or cellular compartments. The aggregation of these proteins is characterized by the formation of extended, β-sheet rich fibrils, terme...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00739
更新日期:2019-10-29 00:00:00
abstract::The first structure of nicotine oxidoreductase (NicA2) was determined by X-ray crystallography. Pseudomonas putida has evolved nicotine-degrading activity to provide a source of carbon and nitrogen. The structure establishes NicA2 as a member of the monoamine oxidase family. Residues 1-50 are disordered and may play a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00963
更新日期:2016-12-06 00:00:00
abstract::Evidence of nonequilibrium local heating in transient spectra of LHCII, the main light-harvesting complex of plants, was studied by using various excitation intensities over a wide temperature range, from 10 K to room temperature. No obvious manifestation of local heating was found at room temperature, whereas at 10 K...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060048a
更新日期:2006-08-08 00:00:00
abstract::Six structural repeat motifs of 58 amino acids are found in the sequence of both mouse and human properdins. Twelve more examples of the motif are available from the sequences of thrombospondin, the terminal complement components, and the thrombospondin-related anonymous protein. The averaged Robson and Chou-Fasman se...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00444a007
更新日期:1989-09-05 00:00:00
abstract::Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401013g
更新日期:2013-10-29 00:00:00
abstract::Recently, it was demonstrated that bifunctional catalase-peroxidases (KatGs) are found not only in archaea and bacteria but also in lower eukaryotes. Structural studies and preliminary biochemical data of the secreted KatG from the rice pathogen Magnaporthe grisea (MagKatG2) suggested both similar and novel features w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00831
更新日期:2015-09-08 00:00:00
abstract::A site-specific analog of ATP, 6,6'-dithiobis (inosinyl imidodiphosphate (S2P-PNP), inactivates the ATPase activities of myosin's proteolytic fragments, heavy meromyosin (HMM) and subfragment one (SF1), by formation of mixed disulfides between the 6 position of the purine ring and certain key cysteines. The stoichiome...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00680a015
更新日期:1975-05-06 00:00:00
abstract::In cerebellum, inositol trisphosphate- (InsP(3)-) gated Ca channels play a key role in learning, though they exhibit a low sensitivity to InsP(3) compared to peripheral tissues. In the present study, the cerebellar InsP(3) receptor is shown to be associated with a novel inhibitor of InsP(3) binding. (3)H-InsP(3) bindi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992175v
更新日期:2000-03-28 00:00:00
abstract::Structural analyses of bacterial thymidylate synthases (TSs) implicate a serine residue corresponding to Ser216 in human TS in hydrogen bond networks that are involved in binding of the nucleotide substrate, 2'-deoxyuridylate (dUMP), and that stabilize a beta-bulge in the protein. Utilizing site-directed mutagenesis, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972562+
更新日期:1998-05-19 00:00:00
abstract::Synaptotagmin I is the calcium sensor in synchronous neurotransmitter release caused by fusion of synaptic vesicles with the presynaptic membrane in neurons. Synaptotagmin I interacts with acidic phospholipids, but also with soluble N-ethylmaleimide-sensitive factor attachment receptors (SNAREs), at various stages in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101864k
更新日期:2011-04-05 00:00:00
abstract::Comparison between the structures and solvent-accessible surfaces of the antigen-binding fragments of two murine anti-p-azophenylarsonate monoclonal antibodies, one bearing a major cross-reactive idiotype of A/J strain mice (36-71) and one lacking the idiotype (R19.9; Lascombe et al., 1989), highlight the structural b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00229a023
更新日期:1991-04-16 00:00:00
abstract::Crystallographic studies of ligand-protein complexes reveal most preferable ligand binding modes, but do not show less populated modes that may contribute to measurable biochemical and biophysical characteristics of the complexes. In some cases, a ligand may bind a protein in essentially different modes. An example is...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047553x
更新日期:2005-05-17 00:00:00
abstract::Activator of G protein signaling 3 (AGS3) activates the Gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors. It competes with Gbetagamma subunits to bind GDP-bound Gi/o(alpha) subunits via four repeated G protein regulatory (GPR) domains in the carboxyl-terminal half of the mol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034561p
更新日期:2003-07-08 00:00:00
abstract::Fully functional variable fragments (Fv) of D1.3, a mouse antibody directed against the hen egg lysozyme, were readily produced as hybrids (Fv-MalE) with the maltose-binding protein of Escherichia coli and purified independently of their antigen-binding properties. We used site-directed mutations of residues in the co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961419y
更新日期:1997-01-07 00:00:00
abstract::Site-directed mutagenesis has been used to replace alanine 305 with phenylalanine (A305F) and serine (A305S) in the active site of cytochrome P450 3A4 (CYP3A4). Enzyme kinetics for diazepam, erythromycin, nifedipine, and testosterone metabolism have been determined for both mutants and wild-type CYP3A4. The A305F muta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992372u
更新日期:2000-04-18 00:00:00
abstract::Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901583n
更新日期:2010-01-26 00:00:00
abstract::Cu,Zn superoxide dismutase from Photobacterium leiognathi has been cloned and expressed in Escherichia coli. The circular dichroism spectrum in the UV region of the recombinant protein indicates an higher content of random coil structure with respect to the eukaryotic enzymes. Investigation of the active site by optic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963020f
更新日期:1997-06-10 00:00:00
abstract::Various omega-halogenated carboxy acids and amides were evaluated as potential active-site-directed reagents for alcohol dehydrogenase. 2-Bromoacetamide and bromoacetic and 3-bromopropionic acids inactivated the enzyme; AMP, NAD+, and NADH markedly decreased the rate of inactivation. Some omega-halogenated carboxyamid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00694a034
更新日期:1975-11-18 00:00:00
abstract::Isothermal titration calorimetry (ITC) measurements of the binding 1-beta carbohydrate-substituted galactopyranoside derivatives to galectin-1 from bovine spleen, a dimer with one binding site per subunit, were performed at 283-285 and 298 K. The disaccharides were lactose, methyl beta-lactoside, lactulose, 4-O-beta-D...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9716478
更新日期:1998-04-28 00:00:00
abstract::Highly purified tryptic fragments of calmodulin were tested for their ability to stimulate adenylate cyclase activity of Bordetella pertussis spheroplast membranes and were compared to their activities on brain Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase. The C-terminal fragment, consisting of residu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00372a025
更新日期:1986-12-02 00:00:00
abstract::Site-directed spin labeling (SDSL), the site-specific incorporation of nitroxide spin-labels into a protein, has allowed us to investigate ligand-induced conformational changes in the ligand-binding domain of human estrogen receptor alpha (hERalpha-LBD). EPR (electron paramagnetic resonance) spectroscopy of the nitrox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035566p
更新日期:2004-02-24 00:00:00
abstract::Isoforms of transforming growth factor beta (TGFbeta) are 25 kDa homodimeric polypeptides that signal by binding and bringing together two related, functionally distinct cell surface receptors designated as TbetaR1 and TbetaR2. Here, we report the solution structure of the 13.8 kDa extracellular domain of human TbetaR...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034366a
更新日期:2003-09-02 00:00:00
abstract::Pantothenate synthetase (PS, EC 6.3.2.1) is the last enzyme in the pantothenate biosynthesis pathway, a metabolic pathway identified as a potential target for new antimicrobials. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Here we report the overexpression, purificati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1004206
更新日期:2010-08-03 00:00:00
abstract::Displacement of the fluorescent substrate analogue methylanthraniloyl ADP (mant-ADP) from kinesin by excess ATP results in a biphasic fluorescent transient. The pH and microtubule dependence of the rates and amplitudes indicates that the two phases are produced by release of bound mant-ADP, with an excess of the 3'-is...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972742j
更新日期:1998-04-14 00:00:00
abstract::Lysyl-tRNA synthetase is a member of the class II aminoacyl-tRNA synthetases and catalyses the specific aminoacylation of tRNA(Lys). The crystal structure of the constitutive lysyl-tRNA synthetase (LysS) from Escherichia coli has been determined to 2.7 A resolution in the unliganded form and in a complex with the lysi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001487r
更新日期:2000-10-24 00:00:00