当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 2. Structural basis of hapten binding and idiotypy.

Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 2. Structural basis of hapten binding and idiotypy.

Abstract：

:Comparison between the structures and solvent-accessible surfaces of the antigen-binding fragments of two murine anti-p-azophenylarsonate monoclonal antibodies, one bearing a major cross-reactive idiotype of A/J strain mice (36-71) and one lacking the idiotype (R19.9; Lascombe et al., 1989), highlight the structural basis for the determination of hapten affinity and idiotypy. Since the sequence of R 19.9 is identical with the germline-encoded sequence at 16 positions in both heavy-chain and light-chain variable regions where somatic mutations and junctional differences have occurred to produce the 36-71 sequence, the structure of R 19.9 can be used to model the structure of the germline-encoded antibody (36-65) in the regions around these sites. These 16 sequence differences exclude the third heavy-chain complementarity-determining region because R 19.9 utilizes a D gene segment not associated with the predominant idiotype, which is 4 residues longer than the canonical D gene segment utilized in the sequences of 36-71 and 36-65. This difference between the structures of R 19.9 and 36-71 does not affect the validity of using the structure of R 19.9 to model the structure of 36-65 since the third heavy-chain complementarity-determining region is highly solvent-exposed in both 36-71 and R 19.9, and does not interact with any of these 16 sites. Comparing the structures of 36-71 and R 19.9 suggests that only three of the differences in the heavy-chain sequences, and three of the differences in the light-chain sequences of 36-71 and 36-65, increase the affinity for hapten.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Strong RK,Petsko GA,Sharon J,Margolies MN

doi

10.1021/bi00229a023

subject

Has Abstract

pub_date

1991-04-16 00:00:00

pages

3749-57

issue

15

eissn

0006-2960

issn

1520-4995

journal_volume

30

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Effect of structural modifications on the activity of the leadzyme.

    abstract：:The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962219p

    authors： Chartrand P,Usman N,Cedergren R

    更新日期：1997-03-18 00:00:00

  • Composite organization of the cobalamin binding and cubilin recognition sites of intrinsic factor.

    abstract：:Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cb...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047936v

    authors： Fedosov SN,Fedosova NU,Berglund L,Moestrup SK,Nexø E,Petersen TE

    更新日期：2005-03-08 00:00:00

  • Phospholipid lateral phase separation and the partition of cis-parinaric acid and trans-parinaric acid among aqueous, solid lipid, and fluid lipid phases.

    abstract：:The partition of cis-parinaric acid (9,11,13,15-cis, trans, trans,cis-octadecatetraenoic acid, cis-PnA) and trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid, trans-PnA) among aqueous, solid lipid, and fluid lipid phases has been measured by three spectroscopic parameters: absorption spectral shifts, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00576a012

    authors： Sklar LA,Miljanich GP,Dratz EA

    更新日期：1979-05-01 00:00:00

  • Ability of DNA and spermidine to affect the activity of restriction endonucleases from several bacterial species.

    abstract：:Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00223a035

    authors： Oller AR,Vanden Broek W,Conrad M,Topal MD

    更新日期：1991-03-05 00:00:00

  • Indirect readout of tRNA for aminoacylation.

    abstract：:Aminoacylation of tRNA by aminoacyl-tRNA synthetases is the essential reaction that matches protein amino acids with the trinucleotide sequences specified in mRNA. Direct electrostatic interactions made by tRNA synthetases with discriminating functional groups on the tRNA bases have long been known to determine aminoa...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi7014647

    authors： Perona JJ,Hou YM

    更新日期：2007-09-18 00:00:00

  • Catalysis by human leukocyte elastase: proton inventory as a mechanistic probe.

    abstract：:Proton inventories (rate measurements in mixtures of H2O and D2O) were determined for the human leukocyte elastase catalyzed hydrolyses of thiobenzyl esters and p-nitroanilides of the peptides MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The dependencies of k2/Ks on mole fraction of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00379a016

    authors： Stein RL,Strimpler AM,Hori H,Powers JC

    更新日期：1987-03-10 00:00:00

  • Spin equilibrium in human methemoglobin: effects of inositol hexaphosphate and bezafibrate as measured by resonance Raman spectroscopy.

    abstract：:The spin equilibria of several derivatives of human methemoglobin were probed by resonance Raman scattering. The intensity of lines in the Raman spectrum gives a measure of the high-spin (S = 5/2) to low-spin (S = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility meas...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00438a056

    authors： Noble RW,DeYoung A,Rousseau DL

    更新日期：1989-06-13 00:00:00

  • Movement of the Rieske iron-sulfur protein in the p-side bulk aqueous phase: effect of lumenal viscosity on redox reactions of the cytochrome b6f complex.

    abstract：:Based on the atomic structures of the mitochondrial cytochrome bc(1) complex, it has been proposed that the soluble domain of the [2Fe-2S] Rieske iron-sulfur protein (ISP) must rotate by ca. 60 degrees and translate through an appreciable distance between two binding sites, proximal to cytochrome c(1) and to the lumen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9921992

    authors： Heimann S,Ponamarev MV,Cramer WA

    更新日期：2000-03-14 00:00:00

  • Isolation and characterization of human cDNA clones encoding the alpha and the alpha' subunits of casein kinase II.

    abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00488a034

    authors： Lozeman FJ,Litchfield DW,Piening C,Takio K,Walsh KA,Krebs EG

    更新日期：1990-09-11 00:00:00

  • Probing inhibitor-induced conformational changes along the interface between tissue factor and factor VIIa.

    abstract：:Upon injury of a blood vessel, activated factor VII (FVIIa) forms a high-affinity complex with its allosteric regulator, tissue factor (TF), and initiates blood clotting. Active site-inhibited factor VIIa (FVIIai) binds to TF with even higher affinity. We compared the interactions of FVIIai and FVIIa with soluble TF (...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010283n

    authors： Osterlund M,Owenius R,Carlsson K,Carlsson U,Persson E,Lindgren M,Freskgård PO,Svensson M

    更新日期：2001-08-07 00:00:00

  • Structure elucidation of marker glycolipids of alloantigen-activated murine T lymphocytes.

    abstract：:DBA/2 mouse spleen cells were stimulated in vitro by (a) alloantigen (mitomycin-treated CBA/J splenocytes), (b) the T cell mitogen concanavalin A (Con A), and (c) the B cell mitogen lipopolysaccharide (LPS). The cultures were pulsed for 10 h with (14)C-labeled galactose and glucosamine. Radiolabeled glycosphingolipids...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00519a002

    authors： Gruner KR,van Eijk RV,Mühlradt PF

    更新日期：1981-08-04 00:00:00

  • Correlation between the inhibition of the acto-heavy meromyosin ATPase and the binding of tropomyosin to F-actin: effects of Mg2+, KCl, troponin I, and troponin C.

    abstract：:When stoichiometric amounts of tropomyosin (TM) are bound to F-actin in the presence of 2 mM ATP, the MG2+-activated acto-heavy meromyosin (HMM) ATPase is inhibited by about 60% in 5 mM MgCl2-30 mM KCl. If the concentration of MgCl2 is reduced to 1 mM, the inhibition disappears because TM no longer binds to F-actin. I...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00683a025

    authors： Eaton BL,Kominz DR,Eisenberg E

    更新日期：1975-06-17 00:00:00

  • Acid-base properties of ionophore A23187 in methanol-water solutions and bound to unilamellar vesicles of dimyristoylphosphatidylcholine.

    abstract：:The acid-base properties of ionophore A23187 in methanol-water solutions (0--95% w/w) and bound to unilamellar vesicles of dimyristoylphosphatidylcholine were examined by ultraviolet and fluorescence spectroscopy, and the spectral properties for the acidic and basic forms were defined under these conditions. Standard ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00539a023

    authors： Kauffman RF,Taylor RW,Pfeiffer DR

    更新日期：1982-05-11 00:00:00

  • A large solvent isotope effect on protein association thermodynamics.

    abstract：:Solvent reorganization can contribute significantly to the energetics of protein-protein interactions. However, our knowledge of the magnitude of the energetic contribution is limited, in part, by a dearth of quantitative experimental measurements. The biotin repressor forms a homodimer as a prerequisite to DNA bindin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400952m

    authors： Eginton C,Beckett D

    更新日期：2013-09-24 00:00:00

  • 3'-Phosphoadenosine 5'-phosphosulfate binding site of flavonol 3-sulfotransferase studied by affinity chromatography and 31P NMR.

    abstract：:The function of Lys-59, Arg-141, and Arg-277 in PAPS binding and catalysis of the flavonol 3-sulfotransferase was investigated. Affinity chromatography of conservative mutants with PAPS analogues allowed us to determine that Lys-59 interacts with the 5' portion of the nucleotide, while Arg-141 interacts with the 3' po...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982239m

    authors： Marsolais F,Laviolette M,Kakuta Y,Negishi M,Pedersen LC,Auger M,Varin L

    更新日期：1999-03-30 00:00:00

  • Evidence for a nucleotide binding site on the isolated beta subunit from Escherichia coli F1-ATPase. Interaction between nucleotide and aurovertin D binding sites.

    abstract：:The nucleotide binding capacity and affinity of the isolated beta subunit from Escherichia coli F1-ATPase have been studied with radiolabeled ADP and ATP by an equilibrium dialysis technique. Each mole of beta subunit in the presence of EDTA bound 1 mol of ADP or ATP with Kd values of 25 microM and 50-100 microM, resp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00321a048

    authors： Issartel JP,Vignais PV

    更新日期：1984-12-18 00:00:00

  • Specific inhibition of in vitro transcription elongation by triplex-forming oligonucleotide-intercalator conjugates targeted to HIV proviral DNA.

    abstract：:A 16-base pair oligo(purine)-oligo(pyrimidine) sequence present in the coding region of two HIV 1 proviral genes (pol and nef) was chosen as a target for triplex-forming oligonucleotides in in vitro transcription assays. Inhibition of transcription elongation was observed with triplex-forming oligonucleotide-acridine ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952993x

    authors： Giovannangeli C,Perrouault L,Escudé C,Nguyen T,Hélène C

    更新日期：1996-08-13 00:00:00

  • Structural characterization of a (+)-trans-anti-benzo[a]pyrene-DNA adduct using NMR, restrained energy minimization, and molecular dynamics.

    abstract：:The (+)-trans-anti-benzo[a]pyrene adduct formed at the N2 amino group of guanine is the major adduct found after metabolic activation of the ubiquitous carcinogen benzo[a]pyrene. The carcinogenic and mutagenic properties of the (+)-trans-anti-BP adduct, as well as related adducts, have been extensively studied. A DNA ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00010a004

    authors： Fountain MA,Krugh TR

    更新日期：1995-03-14 00:00:00

  • Conformation of immunoglobulin M. 2. Nanosecond fluorescence depolarization analysis of segmental flexibility in anti-epsilon-l-dimethylamino-5-naphthalenesulfonyl-L-lysine anti-immunoglobulin from horse, pig, and shark.

    abstract：:The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00660a033

    authors： Holowka DA,Cathou RE

    更新日期：1976-07-27 00:00:00

  • Triplex formation of chemically modified homopyrimidine oligonucleotides: thermodynamic and kinetic studies.

    abstract：:We have investigated effects of chemical modifications of a third strand on the thermodynamic and kinetic properties of the triplex formation between a 23-bp duplex and each of four kinds of 15-mer chemically modified third strands using isothermal titration calorimetry and interaction analysis system. The chemical mo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990832d

    authors： Torigoe H,Shimizume R,Sarai A,Shindo H

    更新日期：1999-11-02 00:00:00

  • Interaction of ribosomal protein S1 and initiation factor IF3 with the 3' major domain and the decoding site of the 30S subunit of Escherichia coli.

    abstract：:We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00112a007

    authors： Laughrea M,Tam J

    更新日期：1991-12-03 00:00:00

  • Revealing Conformational Transitions in G-Protein-Coupled Receptor Rhodopsin upon Phosphorylation.

    abstract：:G-protein-coupled receptors (GPCRs) have evolved as highly specialized cellular machinery that can dictate biological outcomes in response to diverse stimuli. Specifically, they induce multiple pathway responses upon structural perturbations induced at local protein sites. GPCRs utilize a concurrent strategy involving...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00884

    authors： Jatana N,Aswin SK,Rathore S,Thukral L

    更新日期：2020-01-28 00:00:00

  • Evidence for a recombination-independent pathway for the repair of DNA interstrand cross-links based on a site-specific study with nitrogen mustard.

    abstract：:DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962778w

    authors： Berardini M,Mackay W,Loechler EL

    更新日期：1997-03-25 00:00:00

  • L-685,458, an aspartyl protease transition state mimic, is a potent inhibitor of amyloid beta-protein precursor gamma-secretase activity.

    abstract：:Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0005456

    authors： Shearman MS,Beher D,Clarke EE,Lewis HD,Harrison T,Hunt P,Nadin A,Smith AL,Stevenson G,Castro JL

    更新日期：2000-08-01 00:00:00

  • Solution conformations of GM3 gangliosides containing different sialic acid residues as revealed by NOE-based distance mapping, molecular mechanics, and molecular dynamics calculations.

    abstract：:The conformation of the GM3 ganglioside, Neu5Ac alpha 2-3Gal beta 1-4Glc beta 1-1 Cer, and its analogs containing the Neu5Gc or Neu4Ac5Gc residues (Gc = glycolyl, CH2OHCO) was investigated in Me2SO-d6 solution with the aid of a distance-mapping procedure based on rotating-frame NOE contacts, with hydroxyl protons bein...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00145a014

    authors： Siebert HC,Reuter G,Schauer R,von der Lieth CW,Dabrowski J

    更新日期：1992-08-04 00:00:00

  • Crystal structure of a ternary complex of the alcohol dehydrogenase from Sulfolobus solfataricus.

    abstract：:The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035271b

    authors： Esposito L,Bruno I,Sica F,Raia CA,Giordano A,Rossi M,Mazzarella L,Zagari A

    更新日期：2003-12-16 00:00:00

  • Selective binding to polynucleotides of the hybrid intercalating groove binder bis(pyrrolecarboxamide)-oxazolopyridocarbazole: a molecular modeling study.

    abstract：:In order to further characterize the binding of the hybrid molecule NetOPC [bis(pyrrolecarboxamide)-oxazolopyridocarbazole conjugate] to double-stranded DNA, we have performed a molecular modeling study to investigate the binding modes of the complexes possibly formed between NetOPC and synthetic polynucleotides poly ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00172a018

    authors： Goulaouic H,Carteau S,Subra F,Mouscadet JF,Auclair C,Sun JS

    更新日期：1994-02-15 00:00:00

  • Equilibrium folding studies of tetrameric R67 dihydrofolate reductase.

    abstract：:R67 dihydrofolate reductase (DHFR) is an R-plasmid encoded enzyme that confers resistance to the antibacterial drug trimethoprim. This enzyme is not homologous in sequence or structure to chromosomal DHFRs. Equilibrium folding of tetrameric R67 DHFR was studied and found to be fully reversible. Formation of an inactiv...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00180a018

    authors： Zhuang P,Eisenstein E,Howell EE

    更新日期：1994-04-12 00:00:00

  • Cross-linked dimers with nucleating activity in actin prepared from muscle acetone powder.

    abstract：:A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9914964

    authors： Selden LA,Kinosian HJ,Estes JE,Gershman LC

    更新日期：2000-01-11 00:00:00

  • Chloroplasts biogenesis: detection of divinylprotochlorophyllide ester in higher plants.

    abstract：:It is shown that the protochlorophyllide ester pool of etiolated higher plants is a faithful copy of the protochlorophyllide pool. It is made up of both monovinyl- and divinylprotochlorophyllide esters. Although the two tetrapyrroles exhibited similar emission maxima, they were distinguishable by their Soret excitatio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00562a027

    authors： Belanger FC,Rebeiz CA

    更新日期：1980-10-14 00:00:00