Spectroscopic characterization of recombinant Cu,Zn superoxide dismutase from Photobacterium leiognathi expressed in Escherichia coli: evidence for a novel catalytic copper binding site.

abstract：:The constant fragment of the immunoglobulin light chain in which the intramolecular disulfide bond is reduced (reduced CL fragment) assumes a conformation very similar to that of the intact CL fragment and contains two sulfhydryl groups buried in the interior of the molecule [Goto, Y., & Hamaguchi, K. (1979) J. Bioche...

journal_title：Biochemistry

authors： Goto Y,Hamaguchi K

更新日期：1986-05-20 00:00:00

abstract：:Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...

journal_title：Biochemistry

authors： Czekster CM,Naismith JH

更新日期：2017-04-18 00:00:00

abstract：:The reactions of metmyoglobin (metMb) and methemoglobin (metHb), oxidized to their respective oxoferryl free radical species (.Mb-FeIV = O/.Hb-4FeIV = O) by tert-butyl hydroperoxide (t-BuOOH), with nitric oxide (NO.) were studied by a combination of optical, electron spin resonance (ESR), ionspray mass (MS), fluoresce...

journal_title：Biochemistry

authors： Gorbunov NV,Osipov AN,Day BW,Zayas-Rivera B,Kagan VE,Elsayed NM

更新日期：1995-05-23 00:00:00

abstract：:Peptides corresponding to portions of curaremimetic neurotoxin loop 2 and to a structurally similar segment of rabies virus glycoprotein were synthetically modified in order to gain information on structure-function relationships of neurotoxin loop 2 interactions with the acetylcholine receptor. Binding of synthetic p...

journal_title：Biochemistry

authors： Lentz TL

更新日期：1991-11-12 00:00:00

abstract：:The interaction of oxidized nicotine adenine dinucleotide phosphate dependent isocitrate dehydrogenase (from pig heart) with (RS)-3-bromo-2-ketoglutarate was investigated in an effort to evaluate the reagent's potential as a selective reagent for alpha-ketoglutarate binding sites. The enzyme is rapidly inactivated by ...

journal_title：Biochemistry

authors： Hartman FC

更新日期：1981-02-17 00:00:00

abstract：:We have investigated the kinetic and thermodynamic properties of carbon monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis of CO from heme a(3), the complex recombines with an apparent rate constant of approximately 3...

journal_title：Biochemistry

authors： Aagaard A,Gilderson G,Gomes CM,Teixeira M,Brzezinski P

更新日期：1999-08-03 00:00:00

abstract：:The substrate specificity of the beta-glucosidase (CelB) from the hyperthermophilic archaeon Pyrococcus furiosus, a family 1 glycosyl hydrolase, has been studied at a molecular level. Following crystallization and X-ray diffraction of this enzyme, a 3.3 A resolution structural model has been obtained by molecular repl...

journal_title：Biochemistry

authors： Kaper T,Lebbink JH,Pouwels J,Kopp J,Schulz GE,van der Oost J,de Vos WM

更新日期：2000-05-02 00:00:00

abstract：:Dihydroorotates deuteriated at both C5 and C6 have been prepared and used to probe the mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase. Primary deuterium isotope effects on kcat are observed with both (6RS)-[5(S)-2H]- and (6RS)-[6-2H] dihydroorotates (3 and 6, respectively); these effects are ...

journal_title：Biochemistry

authors： Hines V,Johnston M

更新日期：1989-02-07 00:00:00

abstract：:The structure of a novel plant defensin isolated from the flowers of Petunia hybrida has been determined by (1)H NMR spectroscopy. P. hybrida defensin 1 (PhD1) is a basic, cysteine-rich, antifungal protein of 47 residues and is the first example of a new subclass of plant defensins with five disulfide bonds whose stru...

journal_title：Biochemistry

authors： Janssen BJ,Schirra HJ,Lay FT,Anderson MA,Craik DJ

更新日期：2003-07-15 00:00:00

abstract：:One- and two-dimensional NMR spectroscopy and energy minimization calculations were used to investigate the conformation of a 2-aminofluorene- (AF-) modified model human c-H-ras1 protooncogene codon 61 deoxyoligonucleotide duplex, d(C1-A2-C3-C4-A5- [AF-G6]-G7-A8-A9-C10).-d(G11-T12-T13-C14-C15-T16 -G17-G18-T19-G20), in...

journal_title：Biochemistry

authors： Eckel LM,Krugh TR

更新日期：1994-11-22 00:00:00

abstract：:The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...

journal_title：Biochemistry

authors： Gekko K,Timasheff SN

更新日期：1981-08-04 00:00:00

abstract：:Conformational selection and induced fit are two well-known mechanisms of allosteric protein-ligand interaction. Some proteins, like ubiquitin, have recently been found to exist in multiple conformations at equilibrium, suggesting that the conformational selection may be a general mechanism of interaction, in particul...

journal_title：Biochemistry

authors： Chi CN,Bach A,Engström A,Wang H,Strømgaard K,Gianni S,Jemth P

更新日期：2009-08-04 00:00:00

abstract：:We have analyzed the role of several protein motifs in controlling the membrane association of the xlcaax-1 protein in Xenopus oocytes. Xlcaax-1 is a maternally expressed protein that during development is associated with the basal lateral membrane of polarized epithelial cells. It is enriched in the tubule cells of t...

journal_title：Biochemistry

authors： Kloc M,Li XX,Etkin LD

更新日期：1993-08-17 00:00:00

abstract：:The bleomycins (BLMs), tallysomycins (TLMs), phleomycin, and zorbamycin (ZBM) are members of the BLM family of glycopeptide-derived antitumor antibiotics. The BLM-producing Streptomyces verticillus ATCC15003 and the TLM-producing Streptoalloteichus hindustanus E465-94 ATCC31158 both possess at least two self-resistanc...

journal_title：Biochemistry

authors： Rudolf JD,Bigelow L,Chang C,Cuff ME,Lohman JR,Chang CY,Ma M,Yang D,Clancy S,Babnigg G,Joachimiak A,Phillips GN Jr,Shen B

更新日期：2015-11-17 00:00:00

abstract：:The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...

journal_title：Biochemistry

authors： Drohat AC,Xiao G,Tordova M,Jagadeesh J,Pankiewicz KW,Watanabe KA,Gilliland GL,Stivers JT

更新日期：1999-09-14 00:00:00

abstract：:The binding of bleomycin to DNA in the presence and absence of ferric iron was measured by fluorescence spectroscopy. In millimolar concentrations of tris(hydroxymethyl)aminomethane, pH 7.5, approximately 80% of the bleomycin binds to DNA. Ferric iron seems to have no significant effect on the binding of DNA to bleomy...

journal_title：Biochemistry

authors： Caspary WJ,Lanzo DA,Niziak C

更新日期：1982-01-19 00:00:00

abstract：:The envelope glycoprotein of HIV-1 consists of the surface subunit gp120 and the transmembrane subunit gp41. Binding of gp120 to target cell receptors induces a conformational change in gp41, which then mediates the fusion of viral and cellular membranes. A buried isoleucine (Ile573) in a central trimeric coiled coil ...

journal_title：Biochemistry

authors： Liu J,Shu W,Fagan MB,Nunberg JH,Lu M

更新日期：2001-03-06 00:00:00

abstract：:The requirement for Ca2+ in the Mn(2+)-dependent photoactivation of oxygen evolution was re-evaluated using 17 kDa/24 kDa-less photosystem II (PSII) membranes depleted of (Mn)4-clusters by NH2OH extraction. At optimum conditions (1 mM Mn2+/10 microM 2,6-dichlorphenolindophenol (DCIP)/20 mM Ca2+), the light-induced inc...

journal_title：Biochemistry

authors： Chen C,Kazimir J,Cheniae GM

更新日期：1995-10-17 00:00:00

abstract：:The interactions of two plant annexins, annexin 24(Ca32) from Capsicum annuum and annexin Gh1 from Gossypium hirsutum, with phospholipid membranes have been characterized using liposome-based assays and adsorption to monolayers. These two plant annexins show a preference for phosphatidylserine-containing membranes and...

journal_title：Biochemistry

authors： Dabitz N,Hu NJ,Yusof AM,Tranter N,Winter A,Daley M,Zschörnig O,Brisson A,Hofmann A

更新日期：2005-12-13 00:00:00

abstract：:Nuclease footprinting techniques have been employed to investigate the interaction between the diuretic drug amiloride, a sodium channel blocker with potential therapeutic use in the treatment of cystic fibrosis, and three DNA fragments of defined sequence. Using either DNAse I or micrococcal nuclease as probes, an un...

journal_title：Biochemistry

authors： Bailly C,Cuthbert AW,Gentle D,Knowles MR,Waring MJ

更新日期：1993-03-16 00:00:00

abstract：:Previous studies of P-glycoprotein have demonstrated that its function can be modulated by phosphorylation. In the present study, inhibition of protein kinase C with calphostin C or stauroporine or prolonged treatment with the phorbol ester TPA decreased phosphorylation of P-glycoprotein, and impaired transport of vin...

journal_title：Biochemistry

authors： Bates SE,Lee JS,Dickstein B,Spolyar M,Fojo AT

更新日期：1993-09-07 00:00:00

abstract：:Retinoid X receptors (RXRs) are transcription factors with important functions in embryonic development, metabolic processes, differentiation, and apoptosis. A particular feature of RXRs is their ability to act as obligatory heterodimerization partners of class II nuclear receptors. At the same time, these receptors a...

journal_title：Biochemistry

authors： Belorusova A,Osz J,Petoukhov MV,Peluso-Iltis C,Kieffer B,Svergun DI,Rochel N

更新日期：2016-03-29 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) represent the largest class of drug targets, accounting for more than 40% of marketed drugs; however, discovery efforts for many GPCRs have failed to provide viable drug candidates. Historically, drug discovery efforts have focused on developing ligands that act at the orthosteric s...

journal_title：Biochemistry

authors： Wood MR,Hopkins CR,Brogan JT,Conn PJ,Lindsley CW

更新日期：2011-04-05 00:00:00

abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...

journal_title：Biochemistry

authors： Szegletes T,Mallender WD,Thomas PJ,Rosenberry TL

更新日期：1999-01-05 00:00:00

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00

abstract：:The thermal dependence of the structural stability of membrane-reconstituted yeast cytochrome c oxidase has been studied by using different techniques including high-sensitivity differential scanning calorimetry, differential detergent solubility thermal gel analysis, and enzyme activity measurements. For these studie...

journal_title：Biochemistry

authors： Morin PE,Diggs D,Freire E

更新日期：1990-01-23 00:00:00

abstract：:Vc H-NOX (or VCA0720) is an H-NOX (heme-nitric oxide and oxygen binding) protein from facultative aerobic bacterium Vibrio cholerae. It shares significant sequence homology with soluble guanylyl cyclase (sGC), a NO sensor protein commonly found in animals. Similar to sGC, Vc H-NOX binds strongly to NO and CO with affi...

journal_title：Biochemistry

authors： Wu G,Liu W,Berka V,Tsai AL

更新日期：2013-12-31 00:00:00

abstract：:Succinyl-CoA synthetase of Escherichia coli is an alpha 2 beta 2 protein containing active sites at the interfaces between alpha- and beta-subunits. The alpha-subunit contains a histidine residue that is phosphorylated during the reaction. The beta-subunit binds coenzyme A and probably succinate [see Nishimura, J. S. ...

journal_title：Biochemistry

authors： Ybarra J,Prasad AR,Nishimura JS

更新日期：1986-11-04 00:00:00

abstract：:The covalent modification of chicken liver fatty acid synthase by 4-(1-pyrenyl)butyryl-CoA (PBA-CoA), a fluorescent analogue of acetyl- and malonyl-CoA, has been studied. The binding isotherms and the kinetics of inactivation suggest 2 mol of PBA-CoA/mol of enzyme is specifically incorporated into the enzyme. Two clas...

journal_title：Biochemistry

authors： Anderson VE,Hammes GG

更新日期：1983-06-07 00:00:00

abstract：:The collagens synthesized by Chinese hamster ovary cells have been isolated and characterized. Although these cells produce very small amounts of collagen, at least five distinct collagenous chains could be identified from radiolabeled media and cell extracts after limited pepsin digestion. Two chains were characteriz...

journal_title：Biochemistry

authors： Limeback H,Sodek J,Aubin J

更新日期：1982-09-14 00:00:00