Conformation and stability of the constant fragment of the immunoglobulin light chain containing an intramolecular mercury bridge.

abstract：:The importance of DNA polymerase-DNA minor groove interactions on translesion synthesis (TLS) was examined in vitro using variants of exonuclease-deficient Klenow polymerase and site-specifically modified DNA oligonucleotides. Polymerase variant R668A lacks primer strand interactions, while variant Q849A lacks templat...

journal_title：Biochemistry

authors： Gestl EE,Eckert KA

更新日期：2005-05-10 00:00:00

abstract：:The heme environmental structures of lactoperoxidase (LP) have been studied by the use of hyperfine-shifted proton NMR and optical absorption spectra. The NMR spectra of the enzyme in native and cyanide forms in H2O indicated that the fifth ligand of the heme iron is the histidyl imidazole with an anionic character an...

journal_title：Biochemistry

authors： Shiro Y,Morishima I

更新日期：1986-10-07 00:00:00

abstract：:Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with up to 14% of its genes in the form of...

journal_title：Biochemistry

authors： Gopal B,Madan LL,Betz SF,Kossiakoff AA

更新日期：2005-01-11 00:00:00

abstract：:The existence of temperature optima in enzyme catalysis that occur before protein melting sets in can be described by different types of kinetic models. Such optima cause distinctly curved Arrhenius plots and have, for example, been observed in several cold-adapted enzymes from psychrophilic species. The two main expl...

journal_title：Biochemistry

authors： Åqvist J,Sočan J,Purg M

更新日期：2020-10-13 00:00:00

abstract：:Purified human von Willebrand factor (vWF) was digested with Staphylococcus aureus V-8 protease, and specific domains interacting with platelets were isolated and characterized. Amino acid sequence analysis and sodium dodecyl sulfate gel electrophoresis demonstrated that the digestion proceeded primarily by a single c...

journal_title：Biochemistry

authors： Girma JP,Chopek MW,Titani K,Davie EW

更新日期：1986-06-03 00:00:00

abstract：:The photobleaching pathway of a short-wavelength cone opsin purified in delipidated form (lambda(max) = 425 nm) is reported. The batho intermediate of the violet cone opsin generated at 45 K has an absorption maximum at 450 nm. The batho intermediate thermally decays to the lumi intermediate (lambda(max) = 435 nm) at ...

journal_title：Biochemistry

authors： Kusnetzow A,Dukkipati A,Babu KR,Singh D,Vought BW,Knox BE,Birge RR

更新日期：2001-07-03 00:00:00

abstract：:We report the genetically encoded chemical decaging strategy for protein activation in living bacterial cells. In contrast to the metabolically labile photocaging groups inside Escherichia coli, our chemical decaging strategy that relies on the inverse electron-demand Diels-Alder (iDA) reaction is compatible with the ...

journal_title：Biochemistry

authors： Liu L,Liu Y,Zhang G,Ge Y,Fan X,Lin F,Wang J,Zheng H,Xie X,Zeng X,Chen PR

更新日期：2018-01-30 00:00:00

abstract：:BtuB is a bacterial outer-membrane protein that transports vitamin B(12). Spectroscopic studies using site-directed spin labeling (SDSL) indicate that the N-terminus of BtuB undergoes a dramatic structural change from a docked (folded) to an undocked (unfolded) configuration upon substrate binding. However, this drama...

journal_title：Biochemistry

authors： Fanucci GE,Lee JY,Cafiso DS

更新日期：2003-11-18 00:00:00

abstract：:Ultraviolet irradiation of DNA results in various pyrimidine modifications. We studied the excision of an ultraviolet thymine photoproduct by Escherichia coli endonuclease III and by a preparation of human WI-38 cells. These enzymes cleave UV-irradiated DNA at apyrimidinic sites formed by glycosylic removal of the pho...

journal_title：Biochemistry

authors： Ganguly T,Weems KM,Duker NJ

更新日期：1990-08-07 00:00:00

abstract：:Lactone-hydrolyzing enzymes derived from some Bacillus species are capable of disrupting quorum sensing in bacteria that use N-acyl-l-homoserine lactones (AHLs) as intercellular signaling molecules. Despite the promise of these quorum-quenching enzymes as therapeutic and anti-biofouling agents, the ring opening mechan...

journal_title：Biochemistry

authors： Momb J,Thomas PW,Breece RM,Tierney DL,Fast W

更新日期：2006-11-07 00:00:00

abstract：:DNA polymerase delta from calf thymus has been purified to apparent homogeneity by a new procedure which utilizes hydrophobic interaction chromatography with phenyl-Sepharose at an early step to separate most of the calcium-dependent protease activity from DNA polymerase delta and alpha. The purified enzyme migrates a...

journal_title：Biochemistry

authors： Lee MY,Tan CK,Downey KM,So AG

更新日期：1984-04-24 00:00:00

abstract：:We have analyzed the role of several protein motifs in controlling the membrane association of the xlcaax-1 protein in Xenopus oocytes. Xlcaax-1 is a maternally expressed protein that during development is associated with the basal lateral membrane of polarized epithelial cells. It is enriched in the tubule cells of t...

journal_title：Biochemistry

authors： Kloc M,Li XX,Etkin LD

更新日期：1993-08-17 00:00:00

abstract：:Isotope effects have been measured with Mg2+ as the activator and L-malate labeled with deuterium or tritium at carbon 2 as the substrate over the pH range 4-10. Comparison of the nearly pH-independent deuterium-isotope effect on V/Kmalate of 1.5 with the tritium effect of 2.0 by the method of Northrop (Northrop, D.B....

journal_title：Biochemistry

authors： Schimerlik MI,Grimshaw CE,Cleland WW

更新日期：1977-02-22 00:00:00

abstract：:The solution conformation of an agalactosyl penta-antennary "bisected" N-linked glycan from hen ovomucoid has been determined using a combination of 1H-NMR NOE measurements and restrained molecular dynamics (MD) simulations. The majority of glycosidic linkages exhibited restricted torsional fluctuations about the glob...

journal_title：Biochemistry

authors： Rutherford TJ,Neville DC,Homans SW

更新日期：1995-10-31 00:00:00

abstract：:The HIV-1 fusion peptide serves as a useful model system for understanding viral/target cell fusion, at least to the lipid-mixing stage. Previous solid-state NMR studies have shown that the membrane-bound HIV-1 fusion peptide adopts an extended conformation in a lipid mixture close to that of host cells of the virus. ...

journal_title：Biochemistry

authors： Yang J,Weliky DP

更新日期：2003-10-14 00:00:00

abstract：:West Nile virus (WNV) is a mosquito-borne flavivirus with a rapidly expanding global distribution. Infection can cause severe neurological disease and fatality in humans. Efforts are ongoing to develop antiviral drugs that inhibit the WNV protease, a viral enzyme required for polyprotein processing. Unfortunately, lit...

journal_title：Biochemistry

authors： Garces AP,Watowich SJ

更新日期：2013-10-01 00:00:00

abstract：:Human protein Z is a vitamin K-dependent plasma glycoprotein, deficiency of which leads to a mild bleeding tendency. Protein Z appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. In this study, to characterize the gene for protein Z, its organization and structure...

journal_title：Biochemistry

authors： Fujimaki K,Yamazaki T,Taniwaki M,Ichinose A

更新日期：1998-05-12 00:00:00

abstract：:The structure of the dimeric form of the protein L7/L12 from ribosomes from Escherichia coli was studied by using the heterobifunctional cross-linker 4-(6-formyl-3-azidophenoxy)butyrimidate. The imidate group of the cross-linker reacts very specifically with Lys-51 of L7/L12. Subsequent cross-linking of this modified ...

journal_title：Biochemistry

authors： Maassen JA,Schop EN,Möller W

更新日期：1981-02-17 00:00:00

abstract：:Several fusions between the gene for human insulin-like growth factor I (IGF-I) and the genes for different IgG-binding fragments of staphylococcal protein A were assembled and compared regarding expression, secretion, and purification of the peptide hormone. After IgG affinity purification of the fusion proteins from...

journal_title：Biochemistry

authors： Moks T,Abrahmsén L,Holmgren E,Bilich M,Olsson A,Uhlén M,Pohl G,Sterky C,Hultberg H,Josephson S

更新日期：1987-08-25 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors that exhibit photochromism between two states: a thermally stable dark-adapted state and a metastable light-adapted state with bound linear tetrapyrrole (bilin) chromophores possessing 15Z and 15E configurations, respectively. The photodynamics of canonica...

journal_title：Biochemistry

authors： Kirpich JS,Chang CW,Franse J,Yu Q,Escobar FV,Jenkins AJ,Martin SS,Narikawa R,Ames JB,Lagarias JC,Larsen DS

更新日期：2021-01-13 00:00:00

abstract：:The stereoselectivity of the adenosine cyclic 3',5'-phosphate (cAMP) binding sites on the regulatory subunit of the type II bovine cardiac muscle cAMP-dependent protein kinase was investigated by examining the interactions of (Rp)- and (Sp)-adenosine cyclic 3',5'-phosphorothioates (cAMPS) with these sites. While activ...

journal_title：Biochemistry

authors： O'Brian CA,Roczniak SO,Bramson HN,Baraniak J,Stec WJ,Kaiser ET

更新日期：1982-08-31 00:00:00

abstract：:The phosphorylating of the lysine-rich histone at various stages in the cell cycle has been studied. In rapidly dividing cell populations the lysine-rich histone is phosphorylated rapidly after synthesis and more slowly once bound to the chromosome. The half-life of hydrolysis of such interphase phosphorylation in 5 h...

journal_title：Biochemistry

authors： Balhorn R,Jackson V,Granner D,Chalkey R

更新日期：1975-06-03 00:00:00

abstract：:The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

journal_title：Biochemistry

authors： Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

更新日期：2000-09-05 00:00:00

abstract：:The molecular mechanism of O2 binding to hemoglobin (Hb) and myoglobin (Mb) is a long-standing issue in the field of bioinorganic and biophysical chemistry. The nature of Fe-O2 bond in oxy Hb and Mb had been extensively investigated by resonance Raman spectroscopy, which assigned the Fe-O2 stretching bands at ∼570 cm-...

journal_title：Biochemistry

authors： Ohta T,Shibata T,Kobayashi Y,Yoda Y,Ogura T,Neya S,Suzuki A,Seto M,Yamamoto Y

更新日期：2018-12-04 00:00:00

abstract：:Glutamate151 (E151) has been shown to be catalytically essential for the aminopeptidase from Vibrio proteolyticus (AAP). E151 acts as the general acid/base during the catalytic mechanism of peptide hydrolysis. However, a glutamate residue is not the only residue capable of functioning as a general acid/base during cat...

journal_title：Biochemistry

authors： Bzymek KP,Moulin A,Swierczek SI,Ringe D,Petsko GA,Bennett B,Holz RC

更新日期：2005-09-13 00:00:00

abstract：:Phosphoenolpyruvate carboxykinase (PEPCK) is an essential metabolic enzyme operating in the gluconeogenesis and glyceroneogenesis pathways. Previous work has demonstrated that the enzyme cycles between a catalytically inactive open state and a catalytically active closed state. The transition of the enzyme between the...

journal_title：Biochemistry

authors： Johnson TA,Mcleod MJ,Holyoak T

更新日期：2016-01-26 00:00:00

abstract：:The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...

journal_title：Biochemistry

authors： Rong J,Habuchi H,Kimata K,Lindahl U,Kusche-Gullberg M

更新日期：2001-05-08 00:00:00

abstract：:Cytochrome c binds to cardiolipin (CL) on the inner mitochondrial membrane during the initial stages of apoptosis where it oxidizes CL, promoting its release into the cytoplasm where it initiates apoptosis. Previous work has identified interaction sites on cytochrome c involved in the cytochrome c-CL interaction. The ...

journal_title：Biochemistry

authors： Elmer-Dixon MM,Bowler BE

更新日期：2018-10-02 00:00:00

abstract：:The anaerobic global regulator FNR from Escherichia coli is a [4Fe-4S](2+) cluster-containing dimer that is inactivated by O(2) through disruption of the Fe-S cluster and conversion to the monomeric apoprotein. It was shown that apo-FNR is subject to ClpXP proteolysis, and two recognition sites, amino acids 5-11 and a...

journal_title：Biochemistry

authors： Pan Q,Shan Y,Yan A

更新日期：2012-06-26 00:00:00

abstract：:Hydrogen bonds which form between a hydrogen bond donor and an aromatic ring as acceptor are thought to contribute to the stability and function of proteins. We have tested the function of such an interaction in a highly homologous pair of proteins, cellular retinol-binding protein (CRBP) and cellular retinol-binding ...

journal_title：Biochemistry

authors： Jamison RS,Kakkad B,Ebert DH,Newcomer ME,Ong DE

更新日期：1995-09-05 00:00:00