Abstract:
:Cytochrome c binds to cardiolipin (CL) on the inner mitochondrial membrane during the initial stages of apoptosis where it oxidizes CL, promoting its release into the cytoplasm where it initiates apoptosis. Previous work has identified interaction sites on cytochrome c involved in the cytochrome c-CL interaction. The contributions of the lysines attributed to site A, the anionic site, are studied here to elucidate the relative importance of each for electrostatic interaction of cytochrome c with CL at pH 8, conditions where site A is dominant. A set of single, double, and quadruple lysine to alanine variants of yeast iso-1-cytochrome c, at sequence positions 72, 73, 86, and 87, show that all contribute to the site A-mediated interaction with CL. All variants experience two sequential structural rearrangements as the lipid to protein ratio (LPR) increases. At a low LPR near 10, all variants undergo a small heme-centered structural change detected by Soret circular dichroism. At higher LPRs ranging from 22 to 34, all variants partially unfold as detected by Trp59 emission. The robustness of the mechanism of interaction to sequential neutralization of the four lysines assigned to site A demonstrates that site A is more extensive than previously supposed. The nature of both structural rearrangements also depends on which lysines constitute site A. The peroxidase activity of cytochrome c in the early stages of apoptosis depends on the nature of structural rearrangement near the heme. Thus, the lysines that comprise site A may have evolved to optimize the peroxidase signaling switch.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Elmer-Dixon MM,Bowler BEdoi
10.1021/acs.biochem.8b00704subject
Has Abstractpub_date
2018-10-02 00:00:00pages
5683-5695issue
39eissn
0006-2960issn
1520-4995journal_volume
57pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::By use of a mutational assay employing an octadecamer with a mismatch in the center, it is shown that the introduction of phosphorothioate groups near the 5'-end can protect the mismatch against degradation by the 5'-3'-exonuclease activity of Escherichia coli DNA polymerase I. An optimal level of protection is achiev...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00399a032
更新日期:1987-12-15 00:00:00
abstract::Protein aggregation is associated with a wide range of diseases, and molecular probes that are able to detect a diversity of misfolded protein assemblies are of great importance. The identification of prefibrillar states preceding the formation of well-defined amyloid fibrils is of particular interest both because of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100922r
更新日期:2010-08-17 00:00:00
abstract::Two distinct regions of angiogenin are critical for angiogenic activity: a catalytic site capable of cleaving RNA and a noncatalytic site, encompassing residues 60-68, which may bind to an endothelial cell-surface receptor [Hallahan, T. W., Shapiro, R., & Vallee, B. L. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 2222-222...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00149a036
更新日期:1992-09-01 00:00:00
abstract::In Saccharomyces cerevisiae, the histidine-containing phosphotransfer (HPt) protein YPD1 transfers phosphoryl groups between the three different response regulator domains of SLN1, SSK1, and SKN7 (designated R1, R2, and R3, respectively). Together these proteins form a branched histidine-aspartic acid phosphorelay sys...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048433s
更新日期:2005-01-11 00:00:00
abstract::Laser light scattering has been applied to a systematic study of a heterogeneous solution of tubulin at low temperature--conditions under which tubulin assembly into microtubules does not take place. Methods of analyzing laser light scattering results obtained from solutions containing multiple components are discusse...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00645a020
更新日期:1977-12-27 00:00:00
abstract::Histone tails in nucleosomes play critical roles in regulation of many biological processes, including chromatin compaction, transcription, and DNA repair. Moreover, post-translational modifications, notably lysine acetylation, are crucial to these functions. While the tails have been intensively studied, how the stru...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01208
更新日期:2017-04-11 00:00:00
abstract::The Mn4 complex which is involved in water oxidation in photosystem II (PSII) is known to exhibit two types of EPR signals in the S2 state, one of the five redox states of the enzyme cycle: either a multiline signal (S = 1/2) or a signal at g = 4.1 (S = 3/2 or S= 5/2). The S = 1/2 state can be converted to that respon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9728710
更新日期:1998-03-24 00:00:00
abstract::The processes of liposome solubilization and reconstitution were studied by using n-dodecyl octaethylene glycol monoether (C12E8). The solubilization of large unilamellar liposomes prepared by reverse-phase evaporation was systematically investigated by turbidity, 31P nuclear magnetic resonance, and centrifugation exp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00492a022
更新日期:1990-10-09 00:00:00
abstract::Human uracil DNA glycosylase (hUNG) plays a central role in DNA repair and programmed mutagenesis of Ig genes, requiring it to act on sparsely or densely spaced uracil bases located in a variety of contexts, including U/A and U/G base pairs, and potentially uracils within single-stranded DNA (ssDNA). An interesting qu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301562n
更新日期:2013-04-16 00:00:00
abstract::Nonhomologous end joining (NHEJ) is the primary mechanism by which mammalian cells repair DNA double-strand breaks (DSBs). Proteins known to play a role in NHEJ include the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), the Ku 70/Ku 80 heterodimer (Ku), XRCC4, and DNA ligase IV. One of the main roles of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0524060
更新日期:2006-04-04 00:00:00
abstract::It has been shown (Poole et al., 1991) that deletion of residues 44-49 from the sequence of staphylococcal nuclease (E43 SNase) results in an enzyme (E43 delta SNase) that is significantly more active than D43 SNase, an enzyme that differs from the wild-type enzyme by deletion of a single methylene group. In addition,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00229a006
更新日期:1991-04-16 00:00:00
abstract::A new fluorescence method to measure viscosity in cell cytosol [Fushimi, K., & Verkman, A. S. (1991) J. Cell Biol. 112, 719-725] has been applied to determine fluid-phase viscosity in sea urchin eggs. Freshly harvested eggs from Lytechinus pictus were loaded with the dyes 2,7-bis(2-carboxyethyl)-5-(and-6-)carboxyfluor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00115a600
更新日期:1991-12-24 00:00:00
abstract::Macrophage migration inhibitory factor (MIF) is a pro-inflammatory and tumor-promoting cytokine that occurs in two redox-dependent immunologically distinct conformational isoforms. The disease-related structural isoform of MIF (oxMIF) can be specifically and predominantly detected in the circulation of patients with i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01156
更新日期:2018-03-06 00:00:00
abstract::Avian magnetoreception is assumed to occur in the retina. Although its molecular mechanism is unclear, magnetic field-dependent formation and the stability of radical-containing photointermediate(s) are suggested to play key roles in a hypothesis called the radical pair mechanism. Chicken cryptochrome4 (cCRY4) has bee...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00495
更新日期:2020-09-29 00:00:00
abstract::Stopped-flow experiments obtained in the pre-steady-state time scale of the NAD-malic enzyme reaction exhibit a lag prior to the attainment of steady state. Previous results from isotope effect studies in which the deuterium isotope effect on Vmax decreases to a value of 1 at low pH have been interpreted as suggesting...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00059a007
更新日期:1993-03-02 00:00:00
abstract::The electrostatic binding strength of water-soluble proteins having either an excess positive (cytochrome c) or negative (beta-lactoglobulin) electric charge to oppositely charged supported planar bilayers (SPBs) was studied as a function of the bilayer phase state (fluid or gel phase) by IR-ATR spectroscopy. The bila...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991564m
更新日期:1999-11-16 00:00:00
abstract::X-ray crystal structures of enzyme-ligand complexes are widely believed to mimic states in the catalytic cycle, but this presumption has seldom been carefully scrutinized. In the case of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase (IMPDH), 10 structures of various enzyme-substrate-inhibitor complexes ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101590c
更新日期:2010-12-21 00:00:00
abstract::Cnu is a nucleoid protein that has a high degree of sequence homology with Hha/YmoA family proteins, which bind to chromatin and regulate the expression of Escherichia coli virulence genes in response to changes in temperature or ionic strength. Here, we determined its solution structure and dynamic properties and map...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701914t
更新日期:2008-02-19 00:00:00
abstract::DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully complementary 3WJ and found that it contained unpaired bases at the branchpoint, which is consistent with previous observa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00677
更新日期:2017-09-19 00:00:00
abstract::Deazapurine-containing secondary metabolites comprise a broad range of structurally diverse nucleoside analogues found throughout biology, including various antibiotics produced by species of Streptomyces bacteria and the hypermodified tRNA bases queuosine and archaeosine. Despite early interest in deazapurines as ant...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900400e
更新日期:2009-05-12 00:00:00
abstract::Although the addition of various divalent metals to beta-galactosidase resulted in apparent activation, only Mg2+ and Mn2+ actually did activate. The apparent activation by the other divalent metals was shown to be due to Mg2+ impurities. Calcium did not activate, but experiments suggested that it did bind. Other diva...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00586a005
更新日期:1979-09-18 00:00:00
abstract::Self-assembled nanostructures that are sensitive to environmental stimuli are promising nanomaterials for drug delivery. In this class, disulfide-containing redox-sensitive strategies have gained enormous attention because of their wide applicability and simplicity of nanoparticle design. In the context of nucleic aci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00860
更新日期:2021-01-11 00:00:00
abstract::Ethanol, in vitro, is known to perturb the molecular order of the phospholipids in biological membranes, while chronic ethanol exposure, in vivo, leads to resistance to disordering. Such changes have usually been measured by electron spin resonance, utilizing fatty acid spin probes. The use of such probes is controver...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00346a012
更新日期:1985-12-03 00:00:00
abstract::Esterification of retinol (vitamin A alcohol) with long-chain fatty acids by lecithin-retinol acyltransferase (LRAT) is an important step in both the absorption and storage of vitamin A. Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00144a014
更新日期:1992-07-28 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00350
更新日期:2017-07-25 00:00:00
abstract::The hepatitis delta virus (HDV) ribozyme and HDV-like ribozymes are self-cleaving RNAs found throughout all kingdoms of life. These RNAs fold into a double-nested pseudoknot structure and cleave RNA, yielding 2',3'-cyclic phosphate and 5'-hydroxyl termini. The active site nucleotide C75 has a pK(a) shifted >2 pH units...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100670p
更新日期:2010-08-10 00:00:00
abstract::A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potass...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00649a016
更新日期:1976-02-24 00:00:00
abstract::Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the terminal ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300688k
更新日期:2012-08-07 00:00:00
abstract::Oligodeoxynucleotides modified site-specifically with dG-(+)-trans- and dG-(+)-cis-anti-BPDE (7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) or dG-(-)-trans- and dG-(-)-cis-anti-BPDE were used as templates in primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00080a027
更新日期:1993-07-27 00:00:00
abstract::Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01174
更新日期:2017-08-08 00:00:00