Abstract：
:The Mn4 complex which is involved in water oxidation in photosystem II (PSII) is known to exhibit two types of EPR signals in the S2 state, one of the five redox states of the enzyme cycle: either a multiline signal (S = 1/2) or a signal at g = 4.1 (S = 3/2 or S= 5/2). The S = 1/2 state can be converted to that responsible for the g = 4.1 signal upon the absorption of near-infrared (IR) light [Boussac, A., Girerd, J.-J., and Rutherford, A.W. (1996) Biochemistry 35, 6984-6989]. It is shown here that a third state gives rise to signals at g = 10 and 6. This state is formed by IR illumination of the S = 1/2 state at 65 K, a temperature where IR illumination leads to the loss of the S = 1/2 signal but to no formation of the g = 4.1 state. On the basis of the corresponding decrease of the S = 1/2 state, the new state can be trapped in approximately 40% of the PSII centers. Warming of the sample above 65 K, in the dark, leads to the loss of the g = 10 and 6 resonances with the corresponding appearance of the g = 4.1 signal. It is suggested that the IR-induced conversion of the S = 1/2 state into the g = 4.1 state at 150 K involves the transient formation of the new state. The new state is attributed to a S = 5/2 state of the Mn4 complex (although a S value > 5/2 is also a possibility). Spectral simulations indicate an E/D ratio of -0.05 with D
journal_name
Biochemistryjournal_title
Biochemistryauthors
Boussac A,Un S,Horner O,Rutherford AWdoi
10.1021/bi9728710subject
Has Abstractpub_date
1998-03-24 00:00:00pages
4001-7issue
12eissn
0006-2960issn
1520-4995pii
bi9728710journal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00578a015
更新日期：1979-05-29 00:00:00
abstract：:Most RNA molecules that are endowed with catalytic activity function in the form of ribonucleoproteins within cells. These complexes are frequently large, poorly defined, and difficult to study. As a model system to study biological catalysis by ribonucleoproteins, we have modified the hairpin ribozyme by inserting an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00023a021
更新日期：1995-06-13 00:00:00
abstract：:We have studied the kinetics of transfer of glucocerebroside between phospholipid bilayers by using pyrene and 3H-labeled glucocerebroside incorporated into dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC) bilayers. Pyrene-labeled glucocerebroside (PyrCer) molecules are able to form an e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00535a021
更新日期：1982-03-16 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is a dimeric protein which induces formation of new blood vessels (angiogenesis) through binding to VEGF-receptor-2 tyrosine kinase (VEGFR2 TK) or KDR (kinase insert domain-containing receptor) on the surface of endothelial cells. Angiogenesis has been shown to be essential fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981291f
更新日期：1998-11-24 00:00:00
abstract：:The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00161a024
更新日期：1992-11-24 00:00:00
abstract：:We have examined the DNA damage produced by reaction of peroxyl radicals with human fibroblast DNA. DNA damage consisted of both strand breaks and base modifications. The extent of strand breaks and base modifications induced as a function of peroxyl radical concentration was determined by quantitation of fragment siz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9918994
更新日期：1999-12-14 00:00:00
abstract：:The light-driven enzyme NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), a key regulatory step in the chlorophyll biosynthesis pathway. As POR is light activated, it allows intermediates in the reaction pathway to be observed by initiat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0268448
更新日期：2003-01-21 00:00:00
abstract：:14C-Labeled fluorodinitrobenzene and N-ethylmaleimide have been used as chemical probes of the conformational states of myosin induced by the binding of MgADP and MgATP. The results indicate that in the high-energy conformation, MMgADP-Pi, the essential thiols are protected from modification but their diminished react...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00643a005
更新日期：1977-11-29 00:00:00
abstract：:ATP-dependent Mg2+ accumulation in isolated mitochondria occurs predominantly in the matrix and inner membrane compartments. In mitochondria contaminated with lysosomes, the time course and magnitude of ATP-dependent Mg2+ accumulation are influenced by various cytoplasmic substances, besides substrates of the citric a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00656a013
更新日期：1976-06-01 00:00:00
abstract：:Several strains of Sphingobium chlorophenolicum have been isolated from soil that was heavily contaminated with pentachlorophenol (PCP), a toxic pesticide introduced in the 1930s. S. chlorophenolicum appears to have assembled a poorly functioning pathway for degradation of PCP by patching enzymes recruited via two ind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300261p
更新日期：2012-05-08 00:00:00
abstract：:We have investigated the loss of histone H1 from chromatin [D'Anna, J. A., Gurley, L. R., & Tobey, R. A. (1982) Biochemistry 21, 3991-4001] and the structure of chromatin from Chinese hamster (line CHO) cells blocked in early S phase by sequential use of isoleucine deprivation G1 block and 1 mM hydroxyurea (HU) blocka...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00293a027
更新日期：1983-11-22 00:00:00
abstract：:An A81H variant of yeast iso-1-cytochrome c is prepared to test the hypothesis that the steric size of the amino acid at sequence position 81 of cytochrome c, which has evolved from Ala in yeast to Ile in mammals, slows the dynamics of the opening of the heme crevice. The A81H mutation is used both to increase steric ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501252z
更新日期：2015-03-10 00:00:00
abstract：:The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972827k
更新日期：1998-05-19 00:00:00
abstract：:The protein product of the Methanococcus jannaschii MJ0768 gene has been expressed in Escherichia coli, purified to homogeneity, and shown to catalyze the GTP-dependent addition of two l-glutamates to the l-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F(420)-0) to form F(420)-0-glutamyl-glutama...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034779b
更新日期：2003-08-19 00:00:00
abstract：:We have studied the tridecadeoxynucleotide CGCGAATTACGCG (I), which contains an additional A at position 9 compared to the dodecanucleotide of which the crystal structure has been determined. Sequence I exhibits no distinct melting curve and also has a concentration-dependent pattern of peaks on reverse-phase chromato...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a025
更新日期：1986-11-18 00:00:00
abstract：:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a002
更新日期：1976-10-19 00:00:00
abstract：:Dynamic conformations of two distinct immunoglobulin (Ig) isotypes, murine IgE and human IgG1, were examined with fluorescence resonance energy transfer measurements. The IgE mutant epsilon/C gamma 3* and the IgG1 mutant gamma/C gamma 3* each bind [5-(dimethylamino)naphthalen-1-yl]sulfonyl (DNS) in two identical antig...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00148a004
更新日期：1992-08-25 00:00:00
abstract：:A method for the rapid and quantitative analysis of 5'-terminal oligonucleotides of RNAs made in vitro is described. The method involves synthesis of RNA in the presence of [gamma-32P]ATP or GTP, isolation of the RNA, and digestion with T1 or pancreatic ribonucleases to release labeled 5'-triphosphate termanated oligo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00604a005
更新日期：1978-05-30 00:00:00
abstract：:The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00409a058
更新日期：1988-05-03 00:00:00
abstract：:The interaction of the 2'-phosphate-containing nucleotides (NADP+, NADPH, 2'-phosphoadenosine 5'-diphosphoribose, and adenosine 2',5'-bisphosphate) with NADP+ -specific isocitrate dehydrogenase was studied by using 31P NMR spectroscopy. The separate resonances corresponding to free and bound nucleotides, characteristi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00303a015
更新日期：1984-04-10 00:00:00
abstract：:We have carried out a comparative study of the effect of cholesterol on the thermotropic phase behavior of the distearoyl and dielaidoyl molecular species of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine using high-sensitivity differential scanning calorimetry. For both molecular species of pho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962815j
更新日期：1997-04-22 00:00:00
abstract：:We have investigated the unfolding thermodynamics for incorporating cationic side chains in the Dickerson-Drew dodecamer duplex. Incorporation of two 3-aminopropyl-2'-deoxyuridine residues (one on each self-complementary strand) lowers the stability of the duplex. This reduction is driven by unfavorable heat contribut...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050897i
更新日期：2005-09-20 00:00:00
abstract：:We have examined proteins containing the DNA-binding domain of c-Myb with biophysical methods. This DNA-binding domain consists of three imperfect repeats (R1, R2, and R3) conserved among many species. Our results indicate that the DNA-binding domain forms unspecific and specific complexes with oligodeoxynucleotides. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00252a026
更新日期：1994-12-06 00:00:00
abstract：:Pili are surface-linked virulence factors that play key roles in infection establishment in a variety of pathogenic species. In Gram-positive pathogens, pilus formation requires the action of sortases, dedicated transpeptidases that covalently associate pilus building blocks. In Streptococcus pneumoniae, a major human...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901261y
更新日期：2009-11-10 00:00:00
abstract：:The late intermediates involved in the activation mechanism of bovine rhodopsin are investigated by time-resolved optical absorption spectroscopy. Measurements from 10 μs to 200 ms after photolysis were carried out on membrane suspensions of bovine rhodopsin at a temperature of 15 °C and at pH of 7.3, 8.0, and 8.7. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00687
更新日期：2016-09-13 00:00:00
abstract：:ZFY, a male-associated Zn-finger protein encoded by the human Y chromosome, exhibits a distinctive two-finger repeat: whereas odd-numbered domains fit a general consensus, even-numbered domains exhibit systematic differences. Do these odd and even sequences encode structurally distinct surfaces for DNA recognition? As...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00243a005
更新日期：1991-07-23 00:00:00
abstract：:The Iowa point mutation in apolipoprotein A-I (G26R) leads to a systemic amyloidosis condition, and the Milano mutation (R173C) is associated with hypoalphalipoproteinemia, a reduced plasma level of high-density lipoprotein. To probe the structural effects that lead to these outcomes, we used amide hydrogen-deuterium ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300926j
更新日期：2012-11-06 00:00:00
abstract：:Complex I, a key component of the mitochondrial respiratory chain, exhibits diminished activity as a result of cardiac ischemia/reperfusion. Cardiac ischemia/reperfusion is associated with increases in the levels of mitochondrial Ca(2+) and pro-oxidants. In the current in vitro study, we sought evidence for a mechanis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049803f
更新日期：2004-07-06 00:00:00
abstract：:Recent studies have shown that there can be large differences in the stability of double and triple helical nucleic acid complexes, depending on whether RNA or DNA strands are involved. These differences have been attributed to structural differences in the sugar-phosphate backbone of these two polymers. However, sinc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00012a031
更新日期：1995-03-28 00:00:00
abstract：:A cross-linked tripeptide has been isolated from alkaline hydrolysates of NaB3H4-reduced calf bone collagen. The peptide contains dihydroxylysinonorleucine, the most abundant cross-link in bone collagen, and it has a single N-terminal proline and a single C-terminal valine. These amino acids are in peptide linkage wit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00691a011
更新日期：1975-10-07 00:00:00
