Isolation and structure of a cross-linked tripeptide from calf bone collagen.

abstract：:The two dominant glucolipids in Acholeplasma laidlawii, viz., 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol (MGlcDG) and 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1----2)-O-alpha-D-glucopyranosyl ]- sn-glycerol (DGlcDG), have markedly different phase behavior. MGlcDG has an ability to form nonlamellar phases, wher...

journal_title：Biochemistry

authors： Eriksson PO,Rilfors L,Wieslander A,Lundberg A,Lindblom G

更新日期：1991-05-21 00:00:00

abstract：:Pantothenate kinase (PanK) catalyzes the first step of the universal five-step coenzyme A (CoA) biosynthetic pathway. The recently characterized type III PanK (PanK-III, encoded by the coaX gene) is distinct in sequence, structure and enzymatic properties from both the long-known bacterial type I PanK (PanK-I, exempli...

journal_title：Biochemistry

authors： Yang K,Strauss E,Huerta C,Zhang H

更新日期：2008-02-05 00:00:00

abstract：:Sufiredoxins (Srx) repair the inactivated forms of typical two-Cys peroxiredoxins (Prx) implicated in hydrogen peroxide-mediated cell signaling. The reduction of the cysteine sulfinic acid moiety within the active site of the Prx by Srx involves novel sulfur chemistry and the use of ATP and Mg(2+). The 1.65 A crystal ...

journal_title：Biochemistry

authors： Jönsson TJ,Murray MS,Johnson LC,Poole LB,Lowther WT

更新日期：2005-06-21 00:00:00

abstract：:Sedimentation and gel retardation studies show a stronger interaction of HMG 1 and 2 with negatively supercoiled DNA than with linear, nicked-circular, or positively supercoiled ds-DNA. An apparent unwinding angle of 58 degrees was obtained for HMG 1 and 2 when assayed by protection of negatively supercoiled DNA from ...

journal_title：Biochemistry

authors： Sheflin LG,Fucile NW,Spaulding SW

更新日期：1993-04-06 00:00:00

abstract：:Vitronectin is a multifunctional plasma glycoprotein which may regulate the systems related to protease cascades such as the coagulation, fibrinolysis, and complement systems as well as cell adhesion. Solid-phase assays and affinity chromatography on immobilized glycolipids indicated that vitronectin purified under de...

journal_title：Biochemistry

authors： Yoneda A,Ogawa H,Kojima K,Matsumoto I

更新日期：1998-05-05 00:00:00

abstract：:It has previously been shown that 9S, untransformed progestin, estrogen, androgen, and glucocorticoid receptor complexes in rabbit uterine and liver cytosols contain a 59-kDa protein [Tai, P. K., Maeda, Y., Nakao, K., Wakim, N. G., Duhring, J. L., & Faber, L. E. (1986) Biochemistry 25, 5269-5275]. In this work we show...

journal_title：Biochemistry

authors： Sanchez ER,Faber LE,Henzel WJ,Pratt WB

更新日期：1990-05-29 00:00:00

abstract：:Thymidylate synthetase catalyzes an exchange of tritium of [5-3H]dUMP for protons of water in the absence of CH2-H4folate. The turnover number for this reaction is some 45,000-fold lower than that of dTMP formation and Km is 1.2 X 10(-5) M, similar to the dissociation constant of the enzyme-dUMP complex determined by ...

journal_title：Biochemistry

authors： Pogolotti AL Jr,Weill C,Santi DV

更新日期：1979-06-26 00:00:00

abstract：:An NMR and nuclear Overhauser effect (NOE) analysis of Thermus thermophilus tRNAIle1a is presented. This species contains modifications including s2T54 and s4U8 [Horie, N., Hara-Yokoyama, M., Yokoyama, S., Watanabe, K., Kuchino, Y., Nishimura, S., & Miyazawa, T. (1985) Biochemistry 24, 5711-5715]. All the expected sec...

journal_title：Biochemistry

authors： Choi BS,Redfield AG

更新日期：1986-04-08 00:00:00

abstract：:Previous studies of the low molecular mass family 11 xylanase from Bacillus circulans show that the ionization state of the nucleophile (Glu78, pK(a) 4.6) and the acid/base catalyst (Glu172, pK(a) 6.7) gives rise to its pH-dependent activity profile. Inspection of the crystal structure of BCX reveals that Glu78 and Gl...

journal_title：Biochemistry

authors： Joshi MD,Sidhu G,Nielsen JE,Brayer GD,Withers SG,McIntosh LP

更新日期：2001-08-28 00:00:00

abstract：:Type II polyketides are a class of natural products that include pharmaceutically important aromatic compounds such as the antibiotic tetracycline and antitumor compound doxorubicin. The type II polyketide synthase (PKS) is a complex consisting of 5-10 standalone domains homologous to fatty acid synthase (FAS). Polyke...

journal_title：Biochemistry

authors： Korman TP,Tan YH,Wong J,Luo R,Tsai SC

更新日期：2008-02-19 00:00:00

abstract：:It is well-known, but little understood, that the nucleotide sequences between phased A(4-6)-tracts (at 10-11 bp intervals) have only a slight effect on overall curvature. To explore this phenomenon, we have examined the gel-migration properties of sequences containing both A-tracts as well as G-tracts (i.e., sequence...

journal_title：Biochemistry

authors： Merling A,Sagaydakova N,Haran TE

更新日期：2003-05-06 00:00:00

abstract：:Ethanol, in vitro, is known to perturb the molecular order of the phospholipids in biological membranes, while chronic ethanol exposure, in vivo, leads to resistance to disordering. Such changes have usually been measured by electron spin resonance, utilizing fatty acid spin probes. The use of such probes is controver...

journal_title：Biochemistry

authors： Taraschi TF,Wu A,Rubin E

更新日期：1985-12-03 00:00:00

abstract：:Proteolytic fragments of bovine testis calmodulin were obtained by limited proteolysis with trypsin or thrombin. Cadmium-113 NMR studies showed that the tryptic fragment encompassing Ca2+ binding domains III and IV (TR2C) gave rise to a spectrum identical with that of the native protein. Two thrombic fragments contain...

journal_title：Biochemistry

authors： Andersson A,Forsén S,Thulin E,Vogel HJ

更新日期：1983-05-10 00:00:00

abstract：:Polylysine promoted extensive membrane mixing of liposomes only if the buffer pH was below the pKa of the lysyl residues. This observation suggested that fusion could be regulated in a physiological pH range if the homopolymer of L-histidine was substituted as fusogen. Microgram quantities of polyhistidine were added ...

journal_title：Biochemistry

authors： Uster PS,Deamer DW

更新日期：1985-01-01 00:00:00

abstract：:The virally encoded integrase protein carries out retroviral integration, which requires specific interactions with the two ends of the viral DNA, and also with host DNA that is the target of integration. We attached a photo-cross-linking agent to specific viral and target DNA sites to identify regions of the integras...

journal_title：Biochemistry

authors： Heuer TS,Brown PO

更新日期：1998-05-12 00:00:00

abstract：:The potency of cholesterol to affect the acyl chain order and dynamics of cardiolipin membranes in the liquid-crystalline state was monitored by steady-state and time-resolved fluorescence anisotropy as well as excited-state lifetime measurements with cis- and trans-parinaric acids as probes. Up to a cholesterol mole ...

journal_title：Biochemistry

authors： Gallay J,Vincent M

更新日期：1986-05-06 00:00:00

abstract：:The generation of multiprotein complexes at receptors and adapter proteins is crucial for the activation of intracellular signaling pathways. In this study, we used multiple biochemical and biophysical methods to examine the binding properties of several SH2 and SH3 domain-containing signaling proteins as they interac...

journal_title：Biochemistry

authors： Houtman JC,Higashimoto Y,Dimasi N,Cho S,Yamaguchi H,Bowden B,Regan C,Malchiodi EL,Mariuzza R,Schuck P,Appella E,Samelson LE

更新日期：2004-04-13 00:00:00

abstract：:The kinetics of activation of Glu-plasminogen (Glu-Pg) and Lys77-Pg by two-chain recombinant tissue plasminogen activator (t-PA) were determined in the presence of isolated protein components of the extracellular matrix (ECM) and compared to activation in the presence of fibrinogen and fibrinogen fragments and in the ...

journal_title：Biochemistry

authors： Stack S,Gonzalez-Gronow M,Pizzo SV

更新日期：1990-05-22 00:00:00

abstract：:The effect of Mg2+ on the disposition of myosin cross-bridges was studied on myofibrils and synthetic myosin and rod filaments by employing chymotryptic digestion and chemical cross-linking methods. In the presence of low Mg2+ concentrations (0.1 mM), the proteolytic susceptibility at the heavy meromyosin/light meromy...

journal_title：Biochemistry

authors： Reisler E,Liu J,Cheung P

更新日期：1983-10-11 00:00:00

abstract：:A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...

journal_title：Biochemistry

authors： Volwerk JJ,Mrsny RJ,Patapoff TW,Jost PC,Griffith OH

更新日期：1987-01-27 00:00:00

abstract：:The mechanism by which the heme-containing peroxidase, chloroperoxidase, is able to chlorinate substrates is poorly understood. One approach to advance our understanding of the mechanism of the enzyme is to determine those factors which contribute to its stability. In particular, under alkaline conditions, chloroperox...

journal_title：Biochemistry

authors： Blanke SR,Martinis SA,Sligar SG,Hager LP,Rux JJ,Dawson JH

更新日期：1996-11-19 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...

journal_title：Biochemistry

authors： Meinke C,Solé VA,Pospisil P,Dau H

更新日期：2000-06-20 00:00:00

abstract：:EPR studies have revealed that removal of calcium using citric acid from the site in spinach photosystem II which is coupled to the photosynthetic O2-evolving process produces a structural change in the manganese cluster responsible for water oxidation. If done in the dark, this yields a modified S1' oxidation state w...

journal_title：Biochemistry

authors： Sivaraja M,Tso J,Dismukes GC

更新日期：1989-11-28 00:00:00

abstract：:The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...

journal_title：Biochemistry

authors： Zhou H,Shatz W,Purdy MM,Fera N,Dahlquist FW,Reich NO

更新日期：2007-06-19 00:00:00

abstract：:The incorporation of extensively fluorinated, or fluorous, analogues of hydrophobic amino acids into proteins potentially provides the opportunity to modulate the physicochemical properties of proteins in a predictable manner. On the basis of the properties of small fluorocarbon molecules, extensively fluorinated prot...

journal_title：Biochemistry

authors： Buer BC,de la Salud-Bea R,Al Hashimi HM,Marsh EN

更新日期：2009-11-17 00:00:00

abstract：:We studied the urea-induced unfolding transition of staphylococcal nuclease (SNase) and its five proline mutants (P47A, P47T, P117G, P47T/P117G, and P47A/P117G) [corrected] by peptide and aromatic circular dichroism and aromatic absorption spectroscopy at equilibrium and the refolding-unfolding kinetics of the protein...

journal_title：Biochemistry

authors： Ikura T,Tsurupa GP,Kuwajima K

更新日期：1997-05-27 00:00:00

abstract：:Induced fit has been postulated to be an important component of ligand interactions with proteins, including protein-DNA interactions. We imagined that the entropic cost of induced fit might be highly dependent on the local protein sequence context around critical contact residues. To investigate this question, we ana...

journal_title：Biochemistry

authors： Ribas de Pouplana L,Auld DS,Kim S,Schimmel P

更新日期：1996-06-25 00:00:00

abstract：:Recent crystal structures of the small ribosomal subunit have made it possible to examine the detailed energetics of codon recognition on the ribosome by computational methods. The binding of cognate and near-cognate anticodon stem loops to the ribosome decoding center, with mRNA containing the Phe UUU and UUC codons,...

journal_title：Biochemistry

authors： Almlöf M,Andér M,Aqvist J

更新日期：2007-01-09 00:00:00

abstract：:We have used DNase I footprinting to examine the formation of antiparallel DNA triple helices on DNA fragments containing the homopurine target sites (GGA)2GGX(GGA)2GG.(CCT)2CCZ(CCT)2CC (where X.Z is each base pair in turn), with the GA- and GT-rich oligonucleotides, (GGA)2GGN(GGA)2GG and (GGT)2GGN(GGT)2GG (N = each b...

journal_title：Biochemistry

authors： Chandler SP,Fox KR

更新日期：1996-11-26 00:00:00