Incorporation of cationic chains in the Dickerson-Drew dodecamer: correlation of energetics, structure, and ion and water binding.

abstract：:Fluorescence spectroscopy has been used to monitor the partitioning of a series of exchangeable neutral phospholipid probes, labeled with carbazole, indolyl or diphenylhexatrienyl moieties, between large unilamellar vesicles containing 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), 1,2-dioleoyloxy-3-(trimethylammonio...

journal_title：Biochemistry

authors： Shin TB,Leventis R,Silvius JR

更新日期：1991-07-30 00:00:00

abstract：:Loop 181-197 of human thymidylate synthase (hTS) populates two conformational states. In the first state, Cys195, a residue crucial for catalytic activity, is in the active site (active conformer); in the other conformation, it is about 10 A away, outside the active site (inactive conformer). We have designed and expr...

journal_title：Biochemistry

authors： Gibson LM,Lovelace LL,Lebioda L

更新日期：2008-04-22 00:00:00

abstract：:We review signaling by reactive oxygen species, which is emerging as a major physiological process. However, among the reactive oxygen species, H(2)O(2) best fulfills the requirements of being a second messenger. Its enzymatic production and degradation, along with the requirements for the oxidation of thiols by H(2)O...

journal_title：Biochemistry

authors： Forman HJ,Maiorino M,Ursini F

更新日期：2010-02-09 00:00:00

abstract：:Protein folding in vivo takes place in a highly crowded environment. The resulting excluded volume forces are thought to stabilize folded forms of proteins. In agreement, many in vitro studies have shown that the presence of macromolecular crowding agents increases the stability of folded proteins but often by only a ...

journal_title：Biochemistry

authors： Adén J,Wittung-Stafshede P

更新日期：2014-04-15 00:00:00

abstract：:The PufX membrane protein is essential for photosynthetic growth of Rhodobacter sphaeroides because it is required for multiple-turnover electron transfer under anaerobic conditions [see accompanying article; Barz, W. P., Francia, F., Venturoli, G., Melandri, B. A., Verméglio, A., & Oesterhelt, D. (1995) Biochemistry ...

journal_title：Biochemistry

authors： Barz WP,Verméglio A,Francia F,Venturoli G,Melandri BA,Oesterhelt D

更新日期：1995-11-21 00:00:00

abstract：:Thermodynamics of 39 oligonucleotides with internal G.T mismatches dissolved in 1 M NaCl were determined from UV absorbance versus temperature profiles. These data were combined with literature values of six sequences to derive parameters for 10 linearly independent trimer and tetramer sequences with G.T mismatches an...

journal_title：Biochemistry

authors： Allawi HT,SantaLucia J Jr

更新日期：1997-08-26 00:00:00

abstract：:To probe the mechanism of the reversible DNA phosphodiester bond cleavage and religation mechanism of the type I topoisomerase from vaccinia virus, we have synthesized DNA substrates carrying a single nonbridging Rp- or Sp-phosphorothioate (Ps) modification at the scissile phosphodiester (Pd) bond. Analysis of the ste...

journal_title：Biochemistry

authors： Stivers JT,Jagadeesh GJ,Nawrot B,Stec WJ,Shuman S

更新日期：2000-05-09 00:00:00

abstract：:Studying the evolution of catalytically promiscuous enzymes like those from the N-succinylamino acid racemase/ o-succinylbenzoate synthase (NSAR/OSBS) subfamily can reveal mechanisms by which new functions evolve. Some enzymes in this subfamily have only OSBS activity, while others catalyze OSBS and NSAR reactions. We...

journal_title：Biochemistry

authors： Odokonyero D,McMillan AW,Ramagopal UA,Toro R,Truong DP,Zhu M,Lopez MS,Somiari B,Herman M,Aziz A,Bonanno JB,Hull KG,Burley SK,Romo D,Almo SC,Glasner ME

更新日期：2018-07-03 00:00:00

abstract：:Isolation and characterization of a novel radiation-induced product, i.e., the 8-hydroxyguanine residue, produced in deoxyribonucleic acid (DNA), 2'-deoxyguanosine, and 2'-deoxyguanosine 5'-monophosphate by gamma-irradiation in aqueous solution, are described. For this purpose, gamma-irradiated DNA was first hydrolyze...

journal_title：Biochemistry

authors： Dizdaroglu M

更新日期：1985-07-30 00:00:00

abstract：:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (EC 5.3.3.2) catalyzes the antarafacial [1.3] allylic rearrangement of isopentenyl diphosphate (IPP) to its electrophilic allylic isomer dimethylallyl diphosphate (DMAPP). Active-site thiols at C138 and C139 were recently identified by covalent modification u...

journal_title：Biochemistry

authors： Street IP,Coffman HR,Baker JA,Poulter CD

更新日期：1994-04-12 00:00:00

abstract：:A series of 24-homologated 1,25-dihydroxyvitamin D3 compounds have been chemically synthesized and studied with regard to their activity in inducing differentiation of human promyelocyte HL-60 cells to monocytes and in calcium mobilizing activity in vitamin D deficient rats. Homologation of 1,25-dihydroxyvitamin D3 or...

journal_title：Biochemistry

authors： Perlman K,Kutner A,Prahl J,Smith C,Inaba M,Schnoes HK,DeLuca HF

更新日期：1990-01-09 00:00:00

abstract：:The vanadate cofactor in vanadium chloroperoxidase has been studied using UV-VIS absorption spectroscopy. A band is present in the near-UV that is red-shifted as compared to free vanadate and shifts in both position and intensity upon change in pH. Mutation of vanadate binding residues has a clear effect on the spectr...

journal_title：Biochemistry

authors： Renirie R,Hemrika W,Piersma SR,Wever R

更新日期：2000-02-08 00:00:00

abstract：:The binding of 8-anilino-1-naphthalenesulfonate (ANS) to ciliary dynein ATPase leads to a marked increase in the dye's fluorescence intensity, accompanied by a blue shift in the observed fluorescence emission maximum. We found that dynein has 37 +/- 3 ANS binding sites and that experimentally applied ANS concentration...

journal_title：Biochemistry

authors： Saucier AC,Mariotti S,Anderson SA,Purich DL

更新日期：1985-12-17 00:00:00

abstract：:Incubation of bovine plasma amine oxidase (BPAO) with benzylamine and various p-substituted analogues results in a time-dependent inactivation that is attributable to buildup of the H(2)O(2)-turnover product on the basis of protection afforded by coincubation with catalase. The mechanism of inactivation is distinct fr...

journal_title：Biochemistry

authors： Lee Y,Shepard E,Smith J,Dooley DM,Sayre LM

更新日期：2001-01-23 00:00:00

abstract：:Preprotein translocation in Escherichia coli is mediated by the translocase with SecA as peripheral ATPase and SecY, SecE, and SecG as membrane domain. To facilitate large-scale purification of the SecYEG heterotrimer, SecY was fused at its amino terminus with a hexahistidine tag and co-overexpressed with SecE and Sec...

journal_title：Biochemistry

authors： van der Does C,Manting EH,Kaufmann A,Lutz M,Driessen AJ

更新日期：1998-01-06 00:00:00

abstract：:The kinetics and thermodynamics of Al(III) exchange between aluminum citrate (AlL) and human serum transferrin were investigated in the 7.2-8.9 pH range. The C-site of human serum apotransferrin in interaction with bicarbonate removes Al(III) from Al citrate with an exchange equilibrium constant K1 = (2.0 +/- 0.6) x 1...

journal_title：Biochemistry

authors： Hémadi M,Miquel G,Kahn PH,El Hage Chahine JM

更新日期：2003-03-18 00:00:00

abstract：:Despite the expression of only one type of receptor, there is great variation in the ability of different cell types to discriminate between C5a and its more stable metabolite, C5a des Arg74. The mechanism that underlies this phenomenon is not understood but presumably involves differences in the interaction with the ...

journal_title：Biochemistry

authors： Crass T,Bautsch W,Cain SA,Pease JE,Monk PN

更新日期：1999-07-27 00:00:00

abstract：:The requirement of cytochrome c-550 (PSII-V) in photosystem II (PSII) has been assessed in Synechocystis sp. PCC 6803 containing mutations between Gly-351 and Thr-436 of the loop E domain of the chlorophyll a-binding protein CP47. Six photoautotrophic strains were utilized to compare the effect of removal of either th...

journal_title：Biochemistry

authors： Morgan TR,Shand JA,Clarke SM,Eaton-Rye JJ

更新日期：1998-10-13 00:00:00

abstract：:In the native purple bacterial reaction center (RC), light-driven charge separation utilizes only the A-side cofactors, with the symmetry related B-side inactive. The process is initiated by electron transfer from the excited primary donor (P*) to the A-side bacteriopheophytin (P* --> P+ H(A)-) in approximately 3 ps. ...

journal_title：Biochemistry

authors： Kee HL,Laible PD,Bautista JA,Hanson DK,Holten D,Kirmaier C

更新日期：2006-06-13 00:00:00

abstract：:Coenzyme A (CoA)-transferases catalyze transthioesterification reactions involving acyl-CoA substrates, using an active-site carboxylate to form covalent acyl anhydride and CoA thioester adducts. Mechanistic studies of class I CoA-transferases suggested that acyl-CoA binding energy is used to accelerate rate-limiting ...

journal_title：Biochemistry

authors： Mullins EA,Kappock TJ

更新日期：2012-10-23 00:00:00

abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...

journal_title：Biochemistry

authors： Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

更新日期：1995-07-04 00:00:00

abstract：:In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...

journal_title：Biochemistry

authors： Molle V,Kremer L,Girard-Blanc C,Besra GS,Cozzone AJ,Prost JF

更新日期：2003-12-30 00:00:00

abstract：:Phase-sensitive 2D 1H/1H COSY spectra can be used to identify the structures of individual pure specimens of the aminoglycoside antibiotic amikacin and its N-hemisuccinyl derivatives. However, even at 500 MHz the 2D chemical shift dispersion does not allow for unambiguous assignment of all cross-peaks. By use of 2D re...

journal_title：Biochemistry

authors： Andersen NH,Eaton HL,Nguyen KT,Hartzell C,Nelson RJ,Priest JH

更新日期：1988-04-19 00:00:00

abstract：:The secretory component (SC) isolated from human milk was labeled with 2 mol of the fluorescent thiol reagent N-[7-(dimethylamino)-4-methylcoumarinyl]maleimide (DACM) per mol of SC through the reactive disulfide bond of SC. The binding of the labeled SC to polymeric immunoglobulins was examined by gel filtration by me...

journal_title：Biochemistry

authors： Goto Y,Aki K

更新日期：1984-12-18 00:00:00

abstract：:Pleckstrin homology (PH) domains play a central role in a wide array of signaling pathways by binding second messenger lipids of the phosphatidylinositol phosphate (PIP) lipid family. A given type of PIP lipid is formed in a specific cellular membrane where it is generally a minor component of the bulk lipid mixture. ...

journal_title：Biochemistry

authors： Corbin JA,Dirkx RA,Falke JJ

更新日期：2004-12-28 00:00:00

abstract：:The interaction between the calcium binding and inhibitory components of troponin is central to the regulation of muscle contraction. In this work, two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance (2D-¿1H,15N¿-HSQC NMR) spectroscopy was used to determine the stoichiometry, affinity, an...

journal_title：Biochemistry

authors： Mercier P,Li MX,Sykes BD

更新日期：2000-03-21 00:00:00

abstract：:EPR studies have revealed that removal of calcium using citric acid from the site in spinach photosystem II which is coupled to the photosynthetic O2-evolving process produces a structural change in the manganese cluster responsible for water oxidation. If done in the dark, this yields a modified S1' oxidation state w...

journal_title：Biochemistry

authors： Sivaraja M,Tso J,Dismukes GC

更新日期：1989-11-28 00:00:00

abstract：:Heme oxygenase-1 (HO-1) is an enzyme that catalyzes the oxidative degradation of heme. Since free heme is toxic to cells, rapid degradation of heme is important for maintaining cellular health. There have been useful mechanistic studies of the HO reaction based on crystal structures; however, how HO-1 recognizes heme ...

journal_title：Biochemistry

authors： Harada E,Sugishima M,Harada J,Fukuyama K,Sugase K

更新日期：2015-01-20 00:00:00

abstract：:We have measured the pH-dependent (1)H, (13)C, and (15)N NMR spectra of pyridoxal 5'-phosphate ((13)C(2)-PLP) mixed with equal amounts of either doubly (15)N-labeled diaminopropane, (15)N(α)-labeled l-lysine, or (15)N(ε)-labeled l-lysine as model systems for various intermediates of the transimination reaction in PLP-...

journal_title：Biochemistry

authors： Chan-Huot M,Sharif S,Tolstoy PM,Toney MD,Limbach HH

更新日期：2010-12-28 00:00:00

abstract：:FTIR-difference spectroscopy in combination with site-directed mutagenesis has been used to investigate the role of water during the photocycle of bacteriorhodopsin. At least one water molecule is detected which undergoes an increase in H-bonding during the primary bR-->K phototransition. Bands due to water appear in ...

journal_title：Biochemistry

authors： Fischer WB,Sonar S,Marti T,Khorana HG,Rothschild KJ

更新日期：1994-11-01 00:00:00