Abstract:
:Protein folding in vivo takes place in a highly crowded environment. The resulting excluded volume forces are thought to stabilize folded forms of proteins. In agreement, many in vitro studies have shown that the presence of macromolecular crowding agents increases the stability of folded proteins but often by only a few kJ per mol. Although it should not matter at what position in the transition between folded and unfolded forms the effect of crowding is employed, there have been no studies assessing whether excluded volume forces alone can correctly fold polypeptides that are mostly unfolded. However, some studies have indicated that the effect of crowding becomes larger the more destabilized the protein is (but still being folded), suggesting that the crowding effect may be exaggerated for unfolded proteins. To address this question directly, we turned to a destabilized mutant of protein L that is mostly unfolded in water but can be folded upon addition of salt. We find that the effect of 200 mg/mL Dextran 20 on the folding equilibrium constant for unfolded protein L (ΔΔGU ≈ 2 kJ mol(-1)) matches the crowding effects found on the folded wild type protein and the mutant when prefolded by salt. This result indicates that the excluded volume effect is independent of starting protein stability and that crowding can shift the reaction toward the folded form when the polypeptide is in the transition region between folded and unfolded states.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Adén J,Wittung-Stafshede Pdoi
10.1021/bi500222gsubject
Has Abstractpub_date
2014-04-15 00:00:00pages
2271-7issue
14eissn
0006-2960issn
1520-4995journal_volume
53pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The specificity of valyl-, phenylalanyl-, and tyrosyl-tRNA synthetases from yeast has been examined by a series of stringent tests designed to eliminate the possibility of artefactual interference. Valyl-tRNA synthetase, as well as activating a number of amino acid analogues, will accept alanine, cysteine, isoleucine,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00610a006
更新日期:1978-08-22 00:00:00
abstract::Thermal unfolding of discoidal complexes of apolipoprotein (apo) C-1 with dimyristoyl phosphatidylcholine (DMPC) reveals a novel mechanism of lipoprotein stabilization that is based on kinetics rather than thermodynamics. Far-UV CD melting curves recorded at several heating/cooling rates from 0.047 to 1.34 K/min show ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025588w
更新日期:2002-06-11 00:00:00
abstract::Molecules that bind DNA via threading intercalation show high binding affinity as well as slow dissociation kinetics, properties ideal for the development of anticancer drugs. To this end, it is critical to identify the specific molecular characteristics of threading intercalators that result in optimal DNA interactio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01036
更新日期:2018-02-06 00:00:00
abstract::The deposition of fibrils and amorphous aggregates of transthyretin (TTR) in patient tissues is a hallmark of TTR amyloid disease, but the molecular details of amyloidogenesis are poorly understood. Tetramer dissociation is typically rate-limiting for TTR amyloid fibril formation, so we have used a monomeric variant o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049621l
更新日期:2004-06-15 00:00:00
abstract::The inhibitory effect of phosphorothioate residues, located within one strand of double-stranded DNA, on the hydrolytic activity of the restriction endonuclease EcoRV was investigated. Specific incorporation of a phosphorothioate group at the site of cleavage yielded the sequence 5'-GATsATC-3'. This modified sequence ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00493a008
更新日期:1990-10-16 00:00:00
abstract::Little is known about how a membrane can regulate interactions between transmembrane helices. Here, we show that strong self-interaction of the transmembrane helix of human quiescin sulfhydryl oxidase 2 rests on a motif of conserved amino acids comprising one face of the helix. Atomistic molecular dynamics simulations...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01239
更新日期:2016-03-08 00:00:00
abstract::Equilibrium binding experiments are widely used for the accurate characterization of binding and competitive binding behavior in biological systems. Modern high-throughput discovery efforts in chemical biology rely heavily upon this principle. Here, we derive exact analytical expressions for general competitive bindin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048233g
更新日期:2004-12-28 00:00:00
abstract::Aqueous suspensions of dark-adapted bacteriorhodopsin (bR560) in the purple membrane of Halobacterium halobium are exposed to rapid jumps to high pH. Optical and resonance Raman measurements are carried out by using flow and stationary methods. Above pH congruent to 11.5 bR560 starts to be reversibly converted to a sp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00263a019
更新日期:1982-09-28 00:00:00
abstract::Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model membranes used in this earlier study, the lipid composition of fuso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002030k
更新日期:2001-04-10 00:00:00
abstract::Pyrrolocytosine (PC) and 2-aminopurine (2AP) are fluorescent nucleobase analogues of the DNA nucleobases cytosine and adenine, respectively, and form base pairs with guanine and thymine. Both fluorescent nucleobases are used extensively as probes for local structure in nucleic acids as the fluorescence properties of P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00521
更新日期:2018-07-31 00:00:00
abstract::All CG dinucleotides along exons 5-8 of the p53 tumor suppressor gene contain endogenous 5-methylcytosine (MeC). These same sites (e.g., codons 157, 158, 245, 248, and 273) are mutational hot spots in smoking-induced lung cancer. Several groups used the UvrABC endonuclease incision assay to demonstrate that methylated...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035259j
更新日期:2004-01-20 00:00:00
abstract::We have separately expressed the Dictyosteliumdiscoideum myosin II nonhydrolyzer point mutations E459V and E476K [Ruppel, K. M., and Spudich, J. A. (1996) Mol. Biol. Cell 7, 1123-1136] in the soluble myosin head fragment M761-1R [Anson et al. (1996) EMBO J. 15, 6069-6074] and performed transient kinetic analyses to ch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi973143f
更新日期:1998-07-07 00:00:00
abstract::As part of a high-throughput, structural proteomic project we have used NMR spectroscopy to determine the solution structure and ascertain the function of a previously unknown, conserved protein (MtH895) from the thermophilic archeon Methanobacterium thermoautotrophicum. Our findings indicate that MtH895 contains a ce...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0115176
更新日期:2002-04-16 00:00:00
abstract::A manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2 (MndD) cloned in Escherichia coli has been purified to homogeneity. Sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 Da). Steady-state kinetic analysis of Mnd...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951979h
更新日期:1996-01-09 00:00:00
abstract::The interactions of myelin basic protein and peptides derived from it with detergent micelles of lysophosphatidylglycerol, lysophosphatidylserine, palmitoyllysophosphatidic acid, and sodium lauryl sulfate, and with mixed micelles of the neutral detergent dodecylphosphocholine and the negatively charged detergent palmi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00461a014
更新日期:1990-03-06 00:00:00
abstract::Heat shock protein 90 (Hsp90) is a highly conserved ATP-driven machine involved in client protein maturation, folding, and activation. The chaperone is supported by a set of cochaperones that confer client specificities. One of those proteins is the suppressor of G2 allele of skp1 (Sgt1), which participates together w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi5000542
更新日期:2014-04-22 00:00:00
abstract::Solution X-ray scattering patterns of enzyme I of the phosphotransferase system from Staphylococcus carnosus indicate an increase in radius of gyration and molecular mass in the presence of Mg2+ or both Mg2+ and phosphoenolpyruvate, indicating a partial dimerization of enzyme I. Mg2+ ions are essential for both the di...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00048a014
更新日期:1995-12-05 00:00:00
abstract::Isopenicillin N synthase (IPNS) catalyzes the oxidative cyclization of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine to isopenicillin N. It is proposed that the multiple products produced from certain substrate analogues result from pathway branching after formation of a ferryl oxene intermediate. We have been int...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00022a031
更新日期:1995-06-06 00:00:00
abstract::Marijuana is the most widely abused illegal drug, and its spectrum of effects suggests that several receptors are responsible for the activity. Two cannabinoid receptor subtypes, CB1 and CB2, have been identified, but the complex pharmacological properties of exogenous cannabinoids and endocannabinoids are not fully e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200010k
更新日期:2011-06-28 00:00:00
abstract::Raman spectroscopy was used to study the phase behavior of several semisynthetic species of the acidic glycosphingolipid cerebroside sulfate (CBS) which occur in myelin. The C-H stretching mode region at 2800-3100 cm-1 of C18:0-CBS, C24:0-CBS, and C26:0-CBS, and the alpha-hydroxy fatty acid species C18:0h-CBS, was stu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00121a041
更新日期:1992-02-18 00:00:00
abstract::S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner. As dimer formation is crucial to S100 function, we applied a yeast two-hybrid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000257+
更新日期:2000-08-08 00:00:00
abstract::Many proteins utilize segmental motions to catalyze a specific reaction. The Omega loop of triosephosphate isomerase (TIM) is important for preventing the loss of the reactive enediol(ate) intermediate. The loop opens and closes even in the absence of the ligand, and the loop itself does not change conformation during...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049208d
更新日期:2004-09-14 00:00:00
abstract::We describe the crystal structure of human epidermal-type fatty acid binding protein (E-FABP) that was recently found to be highly upregulated in human psoriatic keratinocytes. To characterize E-FABP with respect to ligand-binding properties and tertiary structure, we cloned the respective cDNA, overexpressed the prot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990305u
更新日期:1999-09-21 00:00:00
abstract::The (1)H NMR resonances of the heme substituents of the low-spin Fe(III) form of nitrophorin 2, as its complexes with N-methylimidazole (NP2-NMeIm) and imidazole (NP2-ImH), have been assigned by a combination of (1)H homonuclear two-dimensional NMR techniques and (1)H-(13)C HMQC. Complete assignment of the proton and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026765w
更新日期:2003-01-28 00:00:00
abstract::Salts and polyamines have a variety of effects on the physical properties of DNA, including stabilization against thermal melting. We wished to gain greater insight into the mechanism of this stabilization by ascertaining its effect on the dynamics of base opening and closing reactions, as measured by NMR. Since the b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00404a018
更新日期:1988-02-23 00:00:00
abstract::The interaction between skeletal myosin subfragment 1 (S1) and filamentous actin was examined at various intermediate states of the actomyosin ATPase cycle by chemical cross-linking experiments. Reaction of the actin-S1 complex with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide and N-hydroxysuccinimide generated pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980139a
更新日期:1998-06-09 00:00:00
abstract::Eukaryotic assimilatory nitrate reductase (NR) is a dimeric multidomain molybdo-heme-flavo protein that catalyzes the first and rate-limiting step in the nitrate assimilation of plants, algae, and fungi. Nitrate reduction takes place at the N-terminal molybdenum cofactor-containing domain. Reducing equivalents are der...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1003487
更新日期:2010-09-21 00:00:00
abstract::Minor adducts, derived from the covalent binding of anti-benzo[a]pyrene-7,8-dihydroxy-9,10-epoxide to cellular DNA, may play an important role in generating mutations and initiating cancer. We have applied a combined NMR-computational approach including intensity based refinement to determine the solution structure of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991212f
更新日期:1999-08-17 00:00:00
abstract::We have used nucleoside phosphorothioates (NTP alpha S) and a substitution-interference method to identify phosphate oxygens that appear to be important to guanosine cofactor addition in the self-splicing group I intron from Tetrahymena thermophila. For the majority of these phosphate oxygens, however, the effect of N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00068a001
更新日期:1993-05-04 00:00:00
abstract::Phototropin (phot) is a blue-light sensor protein that elicits several photo responses in plants. Phototropin has two flavin mononucleotide (FMN)-binding domains, LOV1 and LOV2, in its N-terminal half. The C-terminal half is a blue-light-regulated Ser/Thr kinase. Various functional studies have reported that only LOV2...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701851v
更新日期:2008-01-22 00:00:00