Entrapment of Water at the Transmembrane Helix-Helix Interface of Quiescin Sulfhydryl Oxidase 2.

abstract：:The time course of changes in the intravesicular Ca2+ concentration ([Ca2+]i) in terminal cisternal sarcoplasmic reticulum vesicles upon the induction of Ca2+ release was investigated by using tetramethylmurexide (TMX) as an intravesicular Ca2+ probe. Upon the addition of polylysine at the concentration that led to th...

journal_title：Biochemistry

authors： Ikemoto N,Antoniu B,Kang JJ,Mészáros LG,Ronjat M

更新日期：1991-05-28 00:00:00

abstract：:NahE and PhdJ are bifunctional hydratase-aldolases in bacterial catabolic pathways for naphthalene and phenanthrene, respectively. Bacterial species with these pathways can use polycyclic aromatic hydrocarbons (PAHs) as sole sources of carbon and energy. Because of the harmful properties of PAHs and their widespread d...

journal_title：Biochemistry

authors： LeVieux JA,Medellin B,Johnson WH Jr,Erwin K,Li W,Johnson IA,Zhang YJ,Whitman CP

更新日期：2018-06-26 00:00:00

abstract：:alpha-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of 1-fucose from the donor sugar guanosine 5'-diphospho-beta-1-fucose (GDP-Fuc) to an acceptor sugar. A secondary isotope effect on the fucosyltransfer reaction with guanosine 5'-diphospho-[1-2H]-beta-1-fucose (GDP-[1-2H]-Fuc) as the substrate was observed...

journal_title：Biochemistry

authors： Murray BW,Wittmann V,Burkart MD,Hung SC,Wong CH

更新日期：1997-01-28 00:00:00

abstract：:The genomic hypervariation of human immunodeficiency virus 1 (HIV-1) could result from misincorporations by the viral reverse transcriptase. We developed an assay for reverse transcriptase fidelity during RNA-dependent as well as DNA-dependent DNA polymerization in vitro. A lacZ alpha RNA fragment transcribed by T3 RN...

journal_title：Biochemistry

authors： Ji JP,Loeb LA

更新日期：1992-02-04 00:00:00

abstract：:A new actinoleukin-like antitumor antibiotic, BBM-928A, has been shown to interact with isolated DNA molecules. BBM-928A contains two substituted quinolines linked by a cyclic decapeptide. Quenching effects of the covalently closed superhelical PM2 DNA on the BBM-928A fluorescence revealed a strong interaction with an...

journal_title：Biochemistry

authors： Huang CH,Mong S,Crooke ST

更新日期：1980-11-25 00:00:00

abstract：:This study highlights the effect of incorporation of locked nucleic acid (LNA) on improving the functional efficacy of DNAzymes against microRNAs (antagomirzymes). DNAzymes were designed against two different sites of miR-27a, which were encompassed both within the precursor and mature form of miRNA. The cleavage and ...

journal_title：Biochemistry

authors： Kaur H,Scaria V,Maiti S

更新日期：2010-11-09 00:00:00

abstract：:Flavo-diiron proteins (FDPs) function as anaerobic nitric oxide scavengers in some microorganisms, catalyzing reduction of nitric to nitrous oxide. The FDP from Thermotoga maritima can be prepared in a deflavinated form with an intact diferric site (deflavo-FDP). Hayashi et al. [(2010) Biochemistry 49, 7040-7049] repo...

journal_title：Biochemistry

authors： Caranto JD,Weitz A,Giri N,Hendrich MP,Kurtz DM Jr

更新日期：2014-09-09 00:00:00

abstract：:Self-assembled nanostructures that are sensitive to environmental stimuli are promising nanomaterials for drug delivery. In this class, disulfide-containing redox-sensitive strategies have gained enormous attention because of their wide applicability and simplicity of nanoparticle design. In the context of nucleic aci...

journal_title：Biochemistry

authors： Dutta K,Das R,Medeiros J,Thayumanavan S

更新日期：2021-01-11 00:00:00

abstract：:The genomic and antigenomic RNAs of hepatitis delta virus are capable of self-cleavage and show no significant sequence similarities to other known self-cleaving RNAs. We have derived an antigenomic delta RNA which cleaves to completion in 15 s in 9 mM magnesium at 37 degrees C and is capable of efficient self-cleavag...

journal_title：Biochemistry

authors： Smith JB,Gottlieb PA,Dinter-Gottlieb G

更新日期：1992-10-13 00:00:00

abstract：:The structure and function of iron(II)-ruthenium(II) hybrid hemoglobins alpha(Ru-CO)2 beta(Fe)2 and alpha(Fe)2 beta(Ru-CO)2, which can serve as models for the intermediate species of the oxygenation step in native human adult hemoglobin, were investigated by measuring oxygen equilibrium curves and the Fe(II)-N epsilon...

journal_title：Biochemistry

authors： Ishimori K,Tsuneshige A,Imai K,Morishima I

更新日期：1989-10-17 00:00:00

abstract：:By means of 2D NMR techniques, all backbone resonances in the 1H NMR spectrum of the single-stranded DNA binding protein encoded by gene V of the filamentous phage IKe have been assigned sequence specifically (at pH 4.6, T = 298 K). In addition, a major part of the side chain resonances could be assigned as well. Anal...

journal_title：Biochemistry

authors： van Duynhoven JP,Folkers PJ,Prinse CW,Harmsen BJ,Konings RN,Hilbers CW

更新日期：1992-02-04 00:00:00

abstract：:A 16-mer deoxyribonucleotide was labeled at the 5'-end with x-rhodamine and at the 3'-end with fluorescein. Förster resonance energy transfer was used to determine the distribution, P(R), of donor-acceptor distances for the oligomer in three duplex structures (hybridized to complementary strands having 10, 16, and 24 ...

journal_title：Biochemistry

authors： Parkhurst KM,Parkhurst LJ

更新日期：1995-01-10 00:00:00

abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...

journal_title：Biochemistry

authors： Miao R,Martinho M,Morales JG,Kim H,Ellis EA,Lill R,Hendrich MP,Münck E,Lindahl PA

更新日期：2008-09-16 00:00:00

abstract：:The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...

journal_title：Biochemistry

authors： Seok YJ,Lee BR,Gazdar C,Svenson I,Yadla N,Peterkofsky A

更新日期：1996-01-09 00:00:00

abstract：:Understanding the structural basis for defects in protein function that underlie protein-based genetic diseases is the fundamental requirement for development of therapies. This situation is epitomized by the cystic fibrosis transmembrane conductance regulator (CFTR)-the gene product known to be defective in CF patien...

journal_title：Biochemistry

authors： Cheung JC,Deber CM

更新日期：2008-02-12 00:00:00

abstract：:Two intermediates in the biosynthetic pathway to bacterial menaquinones are o-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid. Cell-free extracts have been prepared from Escherichia coli which catalyze the conversion of labeled o-succinylbenzoic acid to the naphthoic acid and also to menaquinones. The naphthoa...

journal_title：Biochemistry

authors： Bryant RW Jr,Bentley R

更新日期：1976-11-02 00:00:00

abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...

journal_title：Biochemistry

authors： Brautigan DL,Picton C,Fischer EH

更新日期：1980-12-09 00:00:00

abstract：:5-Iodonaphthyl 1-azide (INA) has been previously shown to selectively label, on photolysis, only those proteins in contact with the membrane lipids. Low concentrations (less than 10 microM) of INA added to rat ovarian plasma membranes induced, on photoactivation, a selective and complete loss of the response of the ad...

journal_title：Biochemistry

authors： Raviv Y,Bercovici T,Gitler C,Salomon Y

更新日期：1984-01-31 00:00:00

abstract：:To explore the interactions of triacylglycerol and phospholipid hydrolysis in lipoprotein conversions and remodeling, we compared the activities of lipoprotein and hepatic lipases on human VLDL, IDL, LDL, and HDL2. Triacylglycerol and phospholipid hydrolysis by each enzyme were measured concomitantly in each lipoprote...

journal_title：Biochemistry

authors： Deckelbaum RJ,Ramakrishnan R,Eisenberg S,Olivecrona T,Bengtsson-Olivecrona G

更新日期：1992-09-15 00:00:00

abstract：:Previously, we have shown that 4-hydroxynonenal (4-HNE) induces Fas-mediated apoptosis in HLE B-3 cells through a pathway which is independent of FasL, FADD, procaspase 8, and DISC (Li, J., et al. (2006) Biochemistry 45, 12253-12264). The involvement of Daxx has also been suggested in this pathway, but its role is not...

journal_title：Biochemistry

authors： Sharma R,Sharma A,Dwivedi S,Zimniak P,Awasthi S,Awasthi YC

更新日期：2008-01-08 00:00:00

abstract：:The reaction of cytochrome c oxidase with hydrogen peroxide has been of great value in generating and characterizing oxygenated species of the enzyme that are identical or similar to those formed during turnover of the enzyme with dioxygen. Most previous studies have utilized relatively low peroxide concentrations (mi...

journal_title：Biochemistry

authors： Zaslavsky D,Smirnova IA,Brzezinski P,Shinzawa-Itoh K,Yoshikawa S,Gennis RB

更新日期：1999-11-30 00:00:00

abstract：:A large fraction of a constitutively synthesized polypeptide, comprising 5% of the total Escherichia coli protein, is released when plasmolysed cells are subjected to osmotic shock into ice-cold water. Since the protein is not liberated by the conversion of cells to spheroplasts, it is not a typical periplasmic protei...

journal_title：Biochemistry

authors： Jacobson GR,Takacs BJ,Rosenbusch JP

更新日期：1976-06-01 00:00:00

abstract：:Hepatic mixed-function oxidase metabolism of the ubiquitous pollutant polychlorinated biphenyls (PCBs) is implicated in their toxification and detoxification. We used dichlorobiphenyls (DCBs) as models to investigate the effect of the chloro substituent sites on this metabolism experimentally and by molecular orbital ...

journal_title：Biochemistry

authors： Kaminsky LS,Kennedy MW,Adams SM,Guengerich FP

更新日期：1981-12-22 00:00:00

abstract：:Amphiphiles respond both to polar and to nonpolar solvents. In this paper X-ray diffraction and osmotic stress have been used to examine the phase behavior, the structural dimensions, and the work of deforming the monolayer-lined aqueous cavities formed by mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleo...

journal_title：Biochemistry

authors： Rand RP,Fuller NL,Gruner SM,Parsegian VA

更新日期：1990-01-09 00:00:00

abstract：:Esterification of retinol (vitamin A alcohol) with long-chain fatty acids by lecithin-retinol acyltransferase (LRAT) is an important step in both the absorption and storage of vitamin A. Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular re...

journal_title：Biochemistry

authors： Herr FM,Ong DE

更新日期：1992-07-28 00:00:00

abstract：:The effect of ionic strength, temperature, and divalent cations on the association of myosin with actin was determined in the ultracentrifuge using scanning absorption optics. The association constant (Ka) for the binding of heavy meromyosin (HmM) to F-actin was 1 X 10(7) M-1 at 20 degrees C, in 0.10 M KCl, 0.01 M imi...

journal_title：Biochemistry

authors： Margossian SS,Lowey S

更新日期：1978-12-12 00:00:00

abstract：:The accessibility of the asparagine-linked carbohydrate chains of human thyrotropin (hTSH) and free alpha and beta subunits was investigated by their susceptibility to endoglycosidases H and F as well as to peptide:N-glycosidase F. Iodinated hTSH or subunits were incubated with a commercial enzyme preparation containi...

journal_title：Biochemistry

authors： Ronin C,Papandreou MJ,Canonne C,Weintraub BD

更新日期：1987-09-08 00:00:00

abstract：:The second-order rate constants for the reactions between nitrogen monoxide and oxymyoglobin or oxyhemoglobin, determined by stopped-flow spectroscopy, increase with increasing pH. At pH 7.0 the rates are (43.6 +/- 0.5) x 10(6) M(-1) x s(-1) for oxymyoglobin and (89 +/- 3) x 10(6) M(-1) x s(-1) for oxyhemoglobin (per ...

journal_title：Biochemistry

authors： Herold S,Exner M,Nauser T

更新日期：2001-03-20 00:00:00

abstract：:Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of no...

journal_title：Biochemistry

authors： Longo A,Guanga GP,Rose RB

更新日期：2007-03-20 00:00:00

abstract：:DnaB helicase is responsible for unwinding duplex DNA during chromosomal DNA replication and is an essential component of the DNA replication apparatus in Escherichia coli. We have analyzed the mechanism of binding of single-stranded DNA (ssDNA) by the DnaB x DnaC complex and DnaB helicase. Binding of ssDNA to DnaB he...

journal_title：Biochemistry

authors： Biswas SB,Biswas-Fiss EE

更新日期：2006-09-26 00:00:00