Abstract：
:The effect of ionic strength, temperature, and divalent cations on the association of myosin with actin was determined in the ultracentrifuge using scanning absorption optics. The association constant (Ka) for the binding of heavy meromyosin (HmM) to F-actin was 1 X 10(7) M-1 at 20 degrees C, in 0.10 M KCl, 0.01 M imidazole (pH 7.0), 5 MM potassium phosphate, 1 mM MgCl2, and 0.3 mM ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid. Ka was the same for HMM prepared by trypsin or chymotrypsin. The affinity of subfragment 1 (S1) for actin under the same ionic conditions was 3 X 10(6) M-1. Varying the preparative procedure for S1 had little effect on Ka. The small difference in binding energy between HMM and S1 suggests that either only one head can bind strongly to actin at a time or that free energy is lost during the sterically unfavorable attachment of the two heads to actin.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Margossian SS,Lowey Sdoi
10.1021/bi00618a017subject
Has Abstractpub_date
1978-12-12 00:00:00pages
5431-9issue
25eissn
0006-2960issn
1520-4995journal_volume
17pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The activity of the major isoform of porcine pancreatic phospholipase A2 (PLA2), designated B-PLA2, against micellar substrates is inhibited by heparin. Inhibition is a consequence of binding of the enzyme to heparin, documented by a heparin-induced alteration in the intrinsic fluorescence of B-PLA2 and in the 8-anili...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00101a026
更新日期：1991-09-17 00:00:00
abstract：:A complex derived from chromatin containing one molecule of each of histones H2A, H2B, H3, and H4, termed core protein, was studied by 13C and 1H nuclear magnetic resonance. 13C line widths, when analyzed and compared with those of native and thermally unfolded representative globular proteins, showed that regions of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00632a008
更新日期：1977-06-28 00:00:00
abstract：:We previously isolated a unique anticoagulant protein named IX/X-bp (factor IX/factor X-binding protein) from the venom of the habu snake Trimeresurus flavoviridis. We recently determined its primary structure and found that this protein had a structure homologous to the carbohydrate-recognition domains of C-type lect...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00078a012
更新日期：1993-07-13 00:00:00
abstract：:PhoR of Bacillus subtilis is a histidine sensor-kinase belonging to the family of two-component signal transduction systems. PhoR is responsible for processing the phosphate-starvation signal and providing phosphate input to regulate the level of phosphorylated response regulator, PhoP, which activates/represses Pho r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990658t
更新日期：1999-08-03 00:00:00
abstract：:Triplex-forming oligonucleotides (TFOs) have been shown to bind in a sequence-specific manner to polypurine/polypyrimidine sequences in several human gene promoters, including the c-myc P1 promoter. TFOs have been shown to inhibit transcription in vitro and the expression of target genes in cell culture. The human c-m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00025a023
更新日期：1995-06-27 00:00:00
abstract：:A grand canonical formalism is developed to combine discrete simulations for chemically distinct species in equilibrium. Each simulation is based on a perturbed funneled landscape. The formalism is illustrated using the alkaline-induced transitions of cytochrome c as observed by FTIR spectroscopy and with various othe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802293m
更新日期：2009-03-24 00:00:00
abstract：:The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00013
更新日期：2015-04-14 00:00:00
abstract：:Reaction center (RC) complexes isolated from a Zn-bacteriochlorophyll (BChl) a-containing purple bacterium, Acidiphilium rubrum, were characterized by absorption, circular dichroism, and magnetic circular dichroism (MCD) spectroscopy. The oxidized-minus-reduced difference spectra indicated that, in this RC, the Zn-BCh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991444e
更新日期：2000-04-11 00:00:00
abstract：:Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8002956
更新日期：2008-07-08 00:00:00
abstract：:Binding of the coupling ion (Na+ or Li+) and sugars to the purified melibiose permease of Escherichia coli, reconstituted in proteoliposomes, produces selective and cooperative changes of the transporter tryptophan fluorescence. To assess the individual contribution of N- or C-terminal domains of the permease to these...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961372g
更新日期：1996-09-17 00:00:00
abstract：:The time course of changes in the intravesicular Ca2+ concentration ([Ca2+]i) in terminal cisternal sarcoplasmic reticulum vesicles upon the induction of Ca2+ release was investigated by using tetramethylmurexide (TMX) as an intravesicular Ca2+ probe. Upon the addition of polylysine at the concentration that led to th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00235a017
更新日期：1991-05-28 00:00:00
abstract：:Regulation of cellular response is an important mechanism for controlling cellular functions. The transmembrane signaling of the hormone receptors is regulated by GTP-binding proteins (GTPases) and their associated proteins. Our previous studies demonstrated that the bifunctional GTP-binding protein, G alpha h (transg...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9522965
更新日期：1996-02-27 00:00:00
abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00008a014
更新日期：1995-02-28 00:00:00
abstract：:P-Selectin, expressed on activated endothelial cells and platelets, is a high kinetic adhesion receptor involved in leukocyte rolling of the inflammatory response, or in tumor cell binding in the course of metastasis. Thus, P-selectin inhibition is a promising therapeutic target. The anti-inflammatory and anti-metasta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602347g
更新日期：2007-05-22 00:00:00
abstract：:The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00480a026
更新日期：1990-07-17 00:00:00
abstract：:S-(4-Bromo-2,3-dioxobutyl)glutathione (S-BDB-G), a reactive analogue of glutathione, has been synthesized and characterized by UV spectroscopy and thin-layer chromatography, as well as by bromide and primary amine analysis. Incubation of S-BDB-G (200 microM) with the 4-4 isoenzyme of rat liver glutathione S-transferas...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00111a006
更新日期：1991-11-26 00:00:00
abstract：:The gamma-phosphoryl groups of two intermediates (M-ATP and M-ADP-P1) in the pathway of MgATP hydrolysis by myosin undergo extensive oxygen exchange with water. Actin activates the overall rate of hydrolysis at a rate-limiting step which follows these exchange reactions. Thus, actin, by decreasing the turnover time of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00620a022
更新日期：1977-01-11 00:00:00
abstract：:The interaction of the 2'-phosphate-containing nucleotides (NADP+, NADPH, 2'-phosphoadenosine 5'-diphosphoribose, and adenosine 2',5'-bisphosphate) with NADP+ -specific isocitrate dehydrogenase was studied by using 31P NMR spectroscopy. The separate resonances corresponding to free and bound nucleotides, characteristi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00303a015
更新日期：1984-04-10 00:00:00
abstract：:Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501342w
更新日期：2014-12-16 00:00:00
abstract：:The catalytic activity of protein-tyrosine phosphatases (PTPs) is mediated by a cysteine side chain which carries out a nucleophilic attack initiating the phosphate cleavage. Experimentally, it has been observed that the active site cysteine has a remarkably low pKa. In the present study, we have investigated the orig...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971187i
更新日期：1998-04-21 00:00:00
abstract：:The ability of avian-specific linker histone H5, and the globular domains of H5 (GH5) and H1(0) (GH1(0), to self-associate either free in solution or when bound to DNA was investigated. All three proteins underwent a salt-dependent increase in turbidity that may be indicative of nonspecific interactions. Dithiobis(suc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980716v
更新日期：1998-09-08 00:00:00
abstract：:The folding kinetics of a 16-residue beta-hairpin (trpzip4) and five mutants were studied by a laser-induced temperature-jump infrared method. Our results indicate that mutations which affect the strength of the hydrophobic cluster lead to a decrease in the thermal stability of the beta-hairpin, as a result of increas...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052039s
更新日期：2006-02-28 00:00:00
abstract：:Prokaryotic promoters have been extensively studied to relate sequence features to promoter function. Here we examine the relationship between double-helix stability and promoter activity. The double-helix stability is evaluated from sequence data by free energy computation, based on reported values of dinucleotide fr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00414a035
更新日期：1988-07-12 00:00:00
abstract：:Eubacterial peptidyl-tRNA hydrolase (PTH) recycles all N-blocked aminoacyl-tRNA molecules but initiator formyl-methionyl-tRNAfMet, the acceptor helix of which is characterized by a 1-72 mismatch. Positive selection by PTH of noninitiator tRNA molecules with a full 1-72 base pair is abolished, however, upon the removal...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982657r
更新日期：1999-04-20 00:00:00
abstract：:Nitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Dev...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00431
更新日期：2015-07-07 00:00:00
abstract：:Human type-alpha transforming growth factor (hTGF alpha) is a small mitogenic protein containing 50 amino acids and three disulfide bonds. It has both sequence and structural homology with epidermal growth factor (EGF). While the three-dimensional structures of hTGF alpha and other EGF-like proteins have been studied ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00008a003
更新日期：1995-02-28 00:00:00
abstract：:The dissociation of the trypsin-sensitive basic tails of the core histones in core chromatin has been followed as a function of [NaCl] using proton NMR spectroscopy. The tails dissociate in a highly cooperative all or none manner over the salt concentration range 0.2-0.6 M, that is, below the salt concentration requir...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00318a037
更新日期：1984-11-06 00:00:00
abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500139h
更新日期：2014-05-06 00:00:00
abstract：:The fluorescent base analogue 2-aminopurine (2-AP) is commonly used to study specific conformational and protein binding events involving nucleic acids. Here, combinations of steady-state and time-resolved fluorescence spectroscopy of 2-AP were employed to monitor conformational transitions within a model hairpin RNA ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7011977
更新日期：2007-12-11 00:00:00
abstract：:The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00267a039
更新日期：1982-11-23 00:00:00
