Abstract:
:The time course of changes in the intravesicular Ca2+ concentration ([Ca2+]i) in terminal cisternal sarcoplasmic reticulum vesicles upon the induction of Ca2+ release was investigated by using tetramethylmurexide (TMX) as an intravesicular Ca2+ probe. Upon the addition of polylysine at the concentration that led to the maximum rate of Ca2+ release, [Ca2+]i decreased monotonically in parallel with Ca2+ release. Upon induction of Ca2+ release by lower concentrations of polylysine, [Ca2+]i first increased above the resting level, followed by a decrease well below it. The release triggers polylysine, and caffeine brought about dissociation of calcium that bound to a nonvesicular membrane segment consisting of the junctional face membrane and calsequestrin bound to it, as monitored with TMX. No Ca2+ dissociation from calsequestrin-free junctional face membranes or from the dissociated calsequestrin was produced by release triggers, but upon reassociation of the dissociated calsequestrin and the junctional face membrane, Ca2+ dissociation by triggers was restored. On the basis of these results, we propose that the release triggers elicit a signal in the junctional face membrane, presumably in the foot protein moiety, which is then transmitted to calsequestrin, leading to the dissociation of the bound calcium; and in SR vesicles, to the transient increase of [Ca2+]i, and subsequently release across the membrane.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Ikemoto N,Antoniu B,Kang JJ,Mészáros LG,Ronjat Mdoi
10.1021/bi00235a017subject
Has Abstractpub_date
1991-05-28 00:00:00pages
5230-7issue
21eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The solvent accessibility of thrombin in its substrate-free and substrate-bound forms has been compared by amide hydrogen/deuterium (H/(2)H) exchange. The optimized inhibitor peptide dPhe-Pro-Arg chloromethyl ketone (PPACK) was used to simulate the substrate-bound form of thrombin. These studies were motivated by the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0499718
更新日期:2004-05-11 00:00:00
abstract::The binding of 125I-labeled ovine follitropin (oFSH) and 125I-labeled deglycosylated ovine follitropin (DG-oFSH) to porcine granulosa cell membranes was studied at equilibrium and nonequilibrium binding conditions and statistically analyzed. Saturation and competition binding experiments revealed homogeneity in the po...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00386a059
更新日期:1987-06-16 00:00:00
abstract::When the identity of the metal ion activator, M, is changed within the series, Zn2+, Co2+, Mg2+, Ni2+, Mn2+, and Cd2+, the equilibrium distribution among the central complexes in the phosphoglucomutase system is markedly altered. (The central complexes are Ep-M-Glc-6-P, ED-M-Glc-1,6-P2, and Ep-M-Glc-1-P, where Ep and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00663a016
更新日期:1976-09-07 00:00:00
abstract::The protein composition, steady state and time-resolved fluorescence emission spectra were studied in solubilized and aggregated LHCII complexes, that were prepared according to two different isolation protocols: (1) by fractionation of cation-depleted thylakoid membranes using the non-ionic detergent Triton X-100 acc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9625253
更新日期:1997-06-17 00:00:00
abstract::In an effort to further probe metal binding to metallo-beta-lactamase L1 (mbetal L1), Cu- (Cu-L1) and Ni-substituted (Ni-L1) L1 were prepared and characterized by kinetic and spectroscopic studies. Cu-L1 bound 1.7 equiv of Cu and small amounts of Zn(II) and Fe. The EPR spectrum of Cu-L1 exhibited two overlapping, axia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802295z
更新日期:2009-04-07 00:00:00
abstract::Discoidal high-density lipoprotein (HDL) particles are known to be fractionalized into several discrete populations in plasma and to differ in behavior according to size; however, their structural differences and the factors regulating their size are less understood. In this study, we prepared several reconstituted HD...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900785x
更新日期:2009-08-18 00:00:00
abstract::Oxidation of the biological antioxidant alpha-tocopherol (vitamin E; TH) by peroxyl radicals yields 8a-(alkyldioxy)tocopherones, which either may hydrolyze to alpha-tocopheryl quinone (TQ) or may be reduced by ascorbic acid to regenerate TH. To define the chemistry of this putative two-electron TH redox cycle, we stud...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00451a034
更新日期:1989-12-12 00:00:00
abstract::In order to evaluate the contributions that histidine residues might play both in the catalytic activities of endonuclease V and in binding to nontarget DNA, the technique of oligonucleotide site directed mutagenesis was used to create mutations at each of the four histidine residues in the endonuclease V gene. Althou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00246a025
更新日期:1991-08-13 00:00:00
abstract::The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400514k
更新日期:2013-07-02 00:00:00
abstract::The rapid expansion of the amount of genomic and structural data has provided many examples of enzymes with evolutionarily related active sites that catalyze different reactions. Functional comparisons of these active sites can provide insight into the origins of the enormous catalytic proficiency of enzymes and the e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060847t
更新日期:2006-08-15 00:00:00
abstract::Cyclopropane fatty acid (CFA) synthase of Escherichia coli catalyzes a modification of the acyl chains of phospholipid bilayers. We report (i) identification of the CFA synthase protein, (ii) overproduction (> 600-fold) and purification to essential homogeneity of the enzyme, and (iii) the amino acid sequence of CFA s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00160a011
更新日期:1992-11-17 00:00:00
abstract::Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025953j
更新日期:2002-07-02 00:00:00
abstract::We have developed a panel of antibodies specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the rat Na,K-ATPase. TrpE-alpha subunit isoform fusion proteins were used to generate three antisera, each of which reacted specifically with a distinct alpha subunit isotype. Western blot analysis of rat tissue mi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00437a002
更新日期:1989-05-30 00:00:00
abstract::Ppr from the purple phototrophic bacterium, Rhodospirillum centenum (also known as Rhodocista centenaria), is a hybrid of photoactive yellow protein (PYP), bacteriophytochrome (Bph), and histidine kinase (HK) domains. The holo-Ppr (containing both chromophores) exhibits characteristic absorption maxima at 435 nm due t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700616j
更新日期:2007-07-17 00:00:00
abstract::The interaction of insulin with its receptor is complex. Kinetic and equilibrium binding studies suggest coexistence of high- and low-affinity binding sites or negative cooperativity. These phenomena and high-affinity interactions are dependent on the dimeric structure of the receptor. Structure-function studies of in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801693h
更新日期:2008-12-02 00:00:00
abstract::Corticosteroid binding globulin (CBG), a serum glycoprotein which binds glucocorticoids and progestins with high affinity, is widely distributed throughout the animal world. Although its charge and size characteristics have largely been conserved across species, we found the behavior of CBG in squirrel monkey (Saimiri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00407a046
更新日期:1988-04-05 00:00:00
abstract::The aggregation of the microtubule-associated protein, tau, into amyloid fibrils is a hallmark of neurodegenerative diseases such as the tauopathies and Alzheimer's disease. Since monomeric tau is an intrinsically disordered protein and the polymeric fibrils possess an ordered cross-β core, the aggregation process is ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500528x
更新日期:2014-10-21 00:00:00
abstract::GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00516
更新日期:2019-08-20 00:00:00
abstract::The solution structure of the complex formed between an oligodeoxynucleotide containing a two-base bulge (5'-CCATCGTCTACCTTTGGTAGGATGG) and SCA-alpha2, a designed spirocyclic helical molecule, has been elucidated. SCA-alpha2, a close mimic of the metabolite, NCSi-gb, of the DNA bulge-specific enediyne antibiotic neoca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602599d
更新日期:2007-04-24 00:00:00
abstract::An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences gene...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00364a007
更新日期:1986-08-12 00:00:00
abstract::The constant (CL) fragment of the immunoglobulin light chain contains only one intrachain disulfide bond buried in the interior of the molecule. The kinetics of reduction with dithiothreitol of the disulfide bond were studied at various concentrations of guanidine hydrochloride at pH 8.0 and 25 degrees C. It was found...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00356a026
更新日期:1986-04-22 00:00:00
abstract::Six rhodopsin mutants containing disulfide cross-links between different cytoplasmic regions were prepared: disulfide bond 1, between Cys65 (interhelical loop I-II) and Cys316 (end of helix VII); disulfide bond 2, between Cys246 (end of helix VI) and Cys312 (end of helix VII); disulfide bond 3, between Cys139 (end of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9912443
更新日期:1999-09-28 00:00:00
abstract::MAP kinase kinase (MAPKK) is a dual specificity protein kinase that phosphorylates and activates MAP kinase in vivo. In this study, four mouse monoclonal single-chain Fv (scFv) antibodies (Y1-6, Y1-7, Y3-6, and Y3-11) that can specifically bind to Xenopus MAPKK were isolated from combinatorial scFv-displaying phage li...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960956f
更新日期:1996-10-08 00:00:00
abstract::Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00367a061
更新日期:1986-09-23 00:00:00
abstract::Dihydrofolate and dihydropteroylpolyglutamates inhibit pig liver methylenetetrahydrofolate reductase. In all cases the inhibition is linearly competitive with respect to methylenetetrahydrofolate. The Ki values decrease with each additional glutamyl residue from one to six, from a value of 6.5 microM for dihydrofolate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00551a005
更新日期:1980-05-13 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00350
更新日期:2017-07-25 00:00:00
abstract::The X-ray structures of neuronal nitric oxide synthase (nNOS) with N(omega)-hydroxy-l-arginine (l-NHA) and CO (or NO) bound have been determined at 1.91-2.2 A resolution. Microspectrophotometric techniques confirmed reduced redox state and the status of diatomic ligand complexes during X-ray diffraction data collectio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9009743
更新日期:2009-11-03 00:00:00
abstract::RPE65, a protein expressed in cells of the retinal pigment epithelium of the eye, is essential for the synthesis by isomerohydrolase of 11-cis-retinal, the chromophore of rod and cone opsins. Recent work has established that RPE65 is a retinyl ester binding protein, and as all-trans-retinyl esters are the substrate fo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0505363
更新日期:2005-07-26 00:00:00
abstract::The mitochondrial tyrosyl tRNA synthetase from Neurospora crassa (CYT-18 protein) is a bifunctional group I intron splicing cofactor. CYT-18 is capable of splicing multiple group I introns from a wide variety of sources by stabilizing the catalytically active intron structures. CYT-18 and mt TyrRSs from related fungal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401614h
更新日期:2014-03-04 00:00:00
abstract::Two dioxygenases (ARD and ARD') were cloned from Klebsiella pneumoniae that catalyze different oxidative decomposition reactions of an advanced aci-reductone intermediate, CH(3)SCH(2)CH(2)COCH(OH)=CH(OH) (I), in the methionine salvage pathway. The two enzymes are remarkable in that they have the same polypeptide seque...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010110y
更新日期:2001-05-29 00:00:00