Abstract：
:GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on the protein kinase PINK1 and that mimicking the phosphorylation at S111 by a serine/glutamate substitution (S111E) impaired Rab8a activation by its cognate nucleotide exchange factor (GEF) Rabin8. However, Ser111 is not part of the interface of the Rab8a:Rabin8 complex. Here, we performed comparative molecular dynamics and free energy simulations on Rab8a and Rab8a:Rabin8 complexes to elucidate the molecular details of how pS111 and S111E may influence the interaction with Rabin8. The simulations indicate that S111E and pS111 establish an intramolecular interaction with arginine 79 (R79). The interaction persists in the complex and perturbs a favorable intermolecular salt-bridge contact between R79 in Rab8a and aspartate 187 in Rabin8. Binding free energy analysis reveals that S111E and pS111, as well as the R79A mutation, drastically decrease the binding affinity for Rabin8. Combining the R79A mutation with S111E or pS111 nearly diminishes Rab8a-Rabin8 binding. In vitro experiments confirm our computational results showing a >80% decrease in the nucleotide exchange rate of the respective Rab8a mutants in the presence of Rabin8 compared to that of the wild type. In addition to insights into how S111 phosphorylation of Rab8a influences GEF-mediated activation, the simulations demonstrate how side chain modifications in general can allosterically influence the surface side chain interaction network between binding partners.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Pourjafar-Dehkordi D,Vieweg S,Itzen A,Zacharias Mdoi
10.1021/acs.biochem.9b00516subject
Has Abstractpub_date
2019-08-20 00:00:00pages
3546-3554issue
33eissn
0006-2960issn
1520-4995journal_volume
58pub_type
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