Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purification, and studies of the enzyme active site.

abstract：:A pterin analogue, 5-[(3-azido-6-nitrobenzylidene)amino]-2,6-diamino-4-pyrimidinone (ANBADP), was synthesized as a probe of the pterin binding site of phenylalanine hydroxylase. The photoaffinity label has been found to be a competitive inhibitor of the enzyme with respect to 6,7-dimethyltetrahydropterin, having a Ki ...

journal_title：Biochemistry

authors： Gibbs BS,Benkovic SJ

更新日期：1991-07-09 00:00:00

abstract：:The three-dimensional structure of the duplex formed by the association of the unnatural oligonucleotide alpha-d(TCTAAACTC) covalently linked to an acridine derivative (m5Acr) with its natural and parallel complementary sequence beta-d(AGATTTGAG) was investigated by nuclear magnetic resonance spectroscopy and constrai...

journal_title：Biochemistry

authors： Guesnet JL,Vovelle F,Thuong NT,Lancelot G

更新日期：1990-05-22 00:00:00

abstract：:Ankyrin repeat (AR) proteins possess a distinctive modular and repetitive architecture, and their global folds are maintained by short-range interactions in terms of the primary sequence. In this work, we extended our previous study on the highly conserved TPLH tetrapeptide and investigated the impact of a solvent-exp...

journal_title：Biochemistry

authors： Yuan C,Guo Y,Zhu L,Guo W,Mahajan A,Weghorst CM,Li J

更新日期：2013-07-16 00:00:00

abstract：:Vitronectin is a multifunctional plasma glycoprotein which may regulate the systems related to protease cascades such as the coagulation, fibrinolysis, and complement systems as well as cell adhesion. Solid-phase assays and affinity chromatography on immobilized glycolipids indicated that vitronectin purified under de...

journal_title：Biochemistry

authors： Yoneda A,Ogawa H,Kojima K,Matsumoto I

更新日期：1998-05-05 00:00:00

abstract：:The effects of NH3 on the oxygen evolving enzyme have been investigated with EPR and steady-state O2 evolution. The following results were obtained. At low light intensity O2 evolution occurs in all centers even though ammonia is bound. This binding occurs in the S2 state and results in a modification of the multiline...

journal_title：Biochemistry

authors： Boussac A,Rutherford AW,Styring S

更新日期：1990-01-09 00:00:00

abstract：:The solvent polarity dependence of the interaction between thiamin and tryptophan was studied by spectrophotometric methods. The ultraviolet (UV) data clearly indicate that the interaction is weakened when the complex is transferred from water to aqueous ethanol or aqueous dioxane. The interaction of thiamin and trypt...

journal_title：Biochemistry

authors： Farzami B,Mariam YH,Jordan F

更新日期：1977-03-22 00:00:00

abstract：:N-RAP is a recently discovered muscle-specific protein that is concentrated at the myotendon junctions in skeletal muscle and at the intercalated disks in cardiac muscle. The C-terminal half of N-RAP contains a region with sequence homology to nebulin, while a LIM domain is found at its N-terminus. N-RAP is hypothesiz...

journal_title：Biochemistry

authors： Luo G,Herrera AH,Horowits R

更新日期：1999-05-11 00:00:00

abstract：:Apolipophorin III (apoLp-III) from the greater wax moth Galleria mellonella is an exchangeable insect apolipoprotein that consists of five amphipathic alpha-helices, sharing high sequence identity with apoLp-III from the sphinx moth Manduca sexta whose structure is available. To define the minimal requirement for apoL...

journal_title：Biochemistry

authors： Dettloff M,Weers PM,Niere M,Kay CM,Ryan RO,Wiesner A

更新日期：2001-03-13 00:00:00

abstract：:Squid cartilage chondroitin sulfate E (CS-E) exhibits various biological activities, including anticoagulant activities, lymphoid regulatory activities, and neuroregulatory activities [Ueoka, C., Kaneda, N., Okazaki, I., Nadanaka, S., Muramatsu, T., and Sugahara, K. (2000) J. Biol. Chem. 275, 37407-37413]. These activ...

journal_title：Biochemistry

authors： Kinoshita A,Yamada S,Haslam SM,Morris HR,Dell A,Sugahara K

更新日期：2001-10-23 00:00:00

abstract：:We have developed a new genomic sequencing method for detecting, with resolution at the nucleotide level, the interstrand DNA cross-links induced by 4,5',8-trimethylpsoralen along single-copy genes in mammalian cells. The cross-links (diadducts) initially formed are converted into monoadducts by alkali reversal prior ...

journal_title：Biochemistry

authors： Komura J,Ikehata H,Hosoi Y,Riggs AD,Ono T

更新日期：2001-04-03 00:00:00

abstract：:The beta chain 15-42 sequence of the fibrin(ogen) E region was implicated in heparin binding [Odrljin et al. (1996) Blood 88, 2050-2061]; whether heparin binds to other fibrin(ogen) regions remains to be clarified. To address this question, we studied the interaction of heparin with fibrinogen, fibrin, and their major...

journal_title：Biochemistry

authors： Yakovlev S,Gorlatov S,Ingham K,Medved L

更新日期：2003-07-01 00:00:00

abstract：:A series of human lymphoblastoid cell lines derived from nonleukemic donors are known to be cysteine prototrophs (cys+), while several lymphoblastoid lines derived from leukemic donors are cysteine auxotrophs (cys-). We have tested representative cell lines of each type for their content of cystathionase enzyme activi...

journal_title：Biochemistry

authors： Glode LM,Kriegler MP,Livingston DM

更新日期：1981-03-03 00:00:00

abstract：:A rapid assay procedure was developed for cleavage of the N-terminal propeptides of procollagen. With the assay a neutral procollagen N-protease was purified about 300-fold from chick embryo tendon extract. The enzyme had an apparent molecular weight of 260 000 and a pH optimum of 7.4. Ca2+ was required for enzymic ac...

journal_title：Biochemistry

authors： Tuderman L,Kivirikko KI,Prockop DJ

更新日期：1978-07-25 00:00:00

abstract：:In order to further characterize the binding of the hybrid molecule NetOPC [bis(pyrrolecarboxamide)-oxazolopyridocarbazole conjugate] to double-stranded DNA, we have performed a molecular modeling study to investigate the binding modes of the complexes possibly formed between NetOPC and synthetic polynucleotides poly ...

journal_title：Biochemistry

authors： Goulaouic H,Carteau S,Subra F,Mouscadet JF,Auclair C,Sun JS

更新日期：1994-02-15 00:00:00

abstract：:Regulation of cellular response is an important mechanism for controlling cellular functions. The transmembrane signaling of the hormone receptors is regulated by GTP-binding proteins (GTPases) and their associated proteins. Our previous studies demonstrated that the bifunctional GTP-binding protein, G alpha h (transg...

journal_title：Biochemistry

authors： Baek KJ,Das T,Gray CD,Desai S,Hwang KC,Gacchui R,Ludwig M,Im MJ

更新日期：1996-02-27 00:00:00

abstract：:Group II introns self-splice via a two-step mechanism: cleavage at the 5' splice site followed by exon ligation at the 3' splice site. The second step has been difficult to study in vitro because it is generally faster than the first. Herein we describe development and partial kinetic characterization of a novel assay...

journal_title：Biochemistry

authors： Bar-Shalom A,Moore MJ

更新日期：2000-08-22 00:00:00

abstract：:The relaxin-like factor (RLF) is a circulating hormone that is synthesized in the gonads of mammals and released into the bloodstream. The distribution of its receptor and a trace of cross-reactivity to relaxin receptors implied that this relatively new factor is more relaxin- than insulin-like. The chemical synthesis...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：1999-03-09 00:00:00

abstract：:The precise nature of redox modulation of N-methyl-d-aspartate (NMDA) receptors is still unclear, although it is thought to be related to the formation and breaking of disulfide bonds. Recent structural data demonstrated the way in which disulfide bonds in the ligand-binding core of the NR1 subunit are arranged. Howev...

journal_title：Biochemistry

authors： Kaye SL,Sansom MS,Biggin PC

更新日期：2007-02-27 00:00:00

abstract：:Recent structural studies of the HMG-CoA synthase members of the thiolase superfamily have shown that the catalytic loop containing the nucleophilic cysteine follows the phi and psi angle pattern of a II' beta turn. However, the i + 1 residue is conserved as an alanine, which is quite unusual in this position as it mu...

journal_title：Biochemistry

authors： Steussy CN,Robison AD,Tetrick AM,Knight JT,Rodwell VW,Stauffacher CV,Sutherlin AL

更新日期：2006-12-05 00:00:00

abstract：:To probe attitudinal features of the Ca(2+)-deficient site (site I) in the Ca2+ switch of cardiac troponin C (cTnC), we have examined steady-state fluorescence emission and polarization of a Trp26 inserted in a recombinant cardiac TnC (cTnC3.W) and compared these with the properties of the Ca(2+)-competent site I in s...

journal_title：Biochemistry

authors： Rao VG,Akella AB,Su H,Gulati J

更新日期：1995-01-17 00:00:00

abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...

journal_title：Biochemistry

authors： Sha R,Liu F,Iwasaki H,Seeman NC

更新日期：2002-09-10 00:00:00

abstract：:The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...

journal_title：Biochemistry

authors： Westrick JA,Peters KS,Ropp JD,Sligar SG

更新日期：1990-07-17 00:00:00

abstract：:Time-resolved resonance Raman spectroscopy has been employed to monitor geminate heme-CO rebinding in photolyzed HbCO. The excitation frequency was tuned to enhance the scattering from rebound heme sites 20-500 ns subsequent to CO photolysis. The behavior of vFe-C during ligand rebinding has important ramifications co...

journal_title：Biochemistry

authors： Schneebeck MC,Vigil LE,Friedman JM,Chavez MD,Ondrias MR

更新日期：1993-02-09 00:00:00

abstract：:Phospholipids containing a saturated fatty acid in the primary position and an unsaturated fatty acid in the secondary position are a major structural part of biological membranes. The mixed-chain hydrophobic core of the membranes is the diacylglycerol part. To better understand the core properties of membranes we hav...

journal_title：Biochemistry

authors： Di L,Small DM

更新日期：1995-12-26 00:00:00

abstract：:HIV-1 Vif is an accessory protein that induces the proteasomal degradation of the host restriction factor, apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G). The N-terminal half of Vif binds to APOBEC3G, and the C-terminal half binds to subunits of a cullin 5-based ubiquitin ligase. This Vi...

journal_title：Biochemistry

authors： Techtmann SM,Ghirlando R,Kao S,Strebel K,Maynard EL

更新日期：2012-03-13 00:00:00

abstract：:The murine invariant chain (Ii) was found to incorporate radioactive palmitic acid. This binding of fatty acid inhibits the formation of interchain S-S bonds, probably because the cysteine residue in the transmembrane region of the Ii chain is palmitylated. The inhibition of fatty acylation by cerulenin blocks further...

journal_title：Biochemistry

authors： Koch N,Hämmerling GJ

更新日期：1985-10-22 00:00:00

abstract：:The carbohydrate binding specificity of the seed lectin from Artocarpus integrifolia, artocarpin, has been elucidated by the enzyme-linked lectin absorbent assay [Misquith, S., et al (1994) J. Biol. Chem. 269, 30393-30401], wherein it was demonstrated to be a Man/Glc specific lectin with high affinity for the trisacch...

journal_title：Biochemistry

authors： Rani PG,Bachhawat K,Reddy GB,Oscarson S,Surolia A

更新日期：2000-09-05 00:00:00

abstract：:Despite the high degree of sequence similarity in their basic-helix-loop-helix (BHLH) domains, MASH-1 and MyoD are involved in different biological processes. In order to define possible differences between the DNA binding specificities of these two proteins, we investigated the DNA binding properties of MASH-1 by cir...

journal_title：Biochemistry

authors： Meierhan D,el-Ariss C,Neuenschwander M,Sieber M,Stackhouse JF,Allemann RK

更新日期：1995-09-05 00:00:00

abstract：:We have determined the structures of three novel oligosaccharides isolated from human milk using the monoclonal antibody MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. From the results of 500-MHz...

journal_title：Biochemistry

authors： Kitagawa H,Nakada H,Kurosaka A,Hiraiwa N,Numata Y,Fukui S,Funakoshi I,Kawasaki T,Yamashina I,Shimada I

更新日期：1989-10-31 00:00:00

abstract：:The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural r...

journal_title：Biochemistry

authors： Tanaka K,Caaveiro JM,Tsumoto K

更新日期：2015-11-24 00:00:00