Ia-associated invariant chain is fatty acylated before addition of sialic acid.

Abstract:

:The murine invariant chain (Ii) was found to incorporate radioactive palmitic acid. This binding of fatty acid inhibits the formation of interchain S-S bonds, probably because the cysteine residue in the transmembrane region of the Ii chain is palmitylated. The inhibition of fatty acylation by cerulenin blocks further posttranslational maturation of the invariant chain as shown by two-dimensional gel electrophoresis of Ii immunoprecipitates. In particular, the addition of sialic acid residues is blocked. Thus, it appears that fatty acylation is essential for carbohydrate processing of the Ii chain.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Koch N,Hämmerling GJ

doi

10.1021/bi00343a023

subject

Has Abstract

pub_date

1985-10-22 00:00:00

pages

6185-90

issue

22

eissn

0006-2960

issn

1520-4995

journal_volume

24

pub_type

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