Abstract:
:An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences generated during the course of these studies. The largest p36 cDNA insert (p36/6 of 1.6 kilobase pairs) was fully sequenced by the dideoxy method. The DNA sequence of this insert had an open reading frame of 1014 base pairs and coded for a protein with a molecular weight of 38 481. The deduced protein sequence of 338 residues was concordant with 173 residue positions of p36 determined at the protein level. The 5'- and 3'-ends of p36/6 contained 54 and 307 base pairs of untranslated sequence, respectively. Examination of poly(A)+ RNA prepared from the Madin-Darby cell line indicated a p36 mRNA species of about 1.6 kilobases. Four regions of internal homology, each about 70 amino acid residues in length, were observed in the deduced protein sequence for p36. Thirty-three of the 70 residue positions were conserved in at least three of the four repeating units. A comparison of derived amino acid sequence for bovine p36 with that previously determined for human lipocortin [Wallner, B. P., Mattaliano, R. J., Hession, C., Cate, R. L., Tizard, R., Sinclair, L. K., Foeller, C., Chow, E. P., Browning, J. L., Ramachandran, K. L., & Pepinsky, R. B. (1986) Nature (London) 320, 77-81] revealed extensive homology (66% overall) and the presence of four repetitive regions in the lipocortin structure.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kristensen T,Saris CJ,Hunter T,Hicks LJ,Noonan DJ,Glenney JR Jr,Tack BFdoi
10.1021/bi00364a007subject
Has Abstractpub_date
1986-08-12 00:00:00pages
4497-503issue
16eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Folding models suggest that the highly conserved histidine 217 of the cytochrome b subunit from the cytochrome bc1 complex is close to the quinone reductase (Qi) site. This histidine (bH217) in the cytochrome b polypeptide of the photosynthetic bacterium Rhodobacter capsulatus has been replaced with three other residu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00169a014
更新日期:1994-01-25 00:00:00
abstract::The sequence of two overlapping cDNA clones for the zinc metalloproteinase hemorrhagic toxin e (also known as atrolysin e, EC 3.4.24.44) from the venom gland of Crotalus atrox, the Western diamondback rattlesnake, is presented. The assembled cDNA sequence is 1975 nucleotides in length and encodes an open reading frame...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00142a005
更新日期:1992-07-14 00:00:00
abstract::Restrictocin and related fungal endoribonucleases from the α-sarcin family site-specifically cleave the sarcin/ricin loop (SRL) on the ribosome to inhibit translation and ultimately trigger cell death. Previous studies showed that the SRL folds into a bulged-G motif and tetraloop, with restrictocin achieving a specifi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1018336
更新日期:2011-04-12 00:00:00
abstract::Studies are presented of the equilibrium binding of [3H]-d-tubocurarine (dTC) and [3H]acetylcholine (AcCh) to Torpedo postsynaptic membranes. The saturable binding of [3H]dTC is characterized by two affinities: Kd1 = 33 +/- 6 nM and Kd2 = 7.7 +/- 4.6 microM, with equal numbers of binding sites. Both components are com...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00591a032
更新日期:1979-11-27 00:00:00
abstract::Cytochrome P450 BM-3 is a bacterial enzyme with sequence similarity to mammalian P450s that catalyzes the hydroxylation of fatty acids with high efficiency. Enzyme-substrate binding and dynamics has been an important topic of study for cytochromes P450 because most of the crystal structures of substrate-bound structur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4000645
更新日期:2013-10-01 00:00:00
abstract::An investigation into the kinetics and regulatory properties of the type-1 phosphofructokinase (PFK) from the extreme thermophile Thermus thermophilus (TtPFK) reveals an enzyme that is inhibited by PEP and activated by ADP by modifying the affinity exhibited for the substrate fructose 6-phosphate (Fru-6-P) in a manner...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401402j
更新日期:2014-01-14 00:00:00
abstract::Yeast mitochondrial NAD+-specific isocitrate dehydrogenase (IDH) has previously been shown to bind specifically to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions have been found to allosterically inhibit activity of the enzyme. This inhibition is relieved by AMP, an allo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0200662
更新日期:2002-06-04 00:00:00
abstract::A large set of glycome information was obtained from egg white proteins of 88 samples from Galloanserae (63 Anseriformes and 25 Galliformes). The data were obtained on whole N-glycan structures and types of sialic acids of these egg whites by glycoblotting-based high-throughput and quantitative glycomics. The results ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101940x
更新日期:2011-05-31 00:00:00
abstract::The pyridoxal 5'-phosphate dependent histidine decarboxylase from Morganella morganii shows a nitrogen isotope effect k14/k15 = 0.9770 +/- 0.0021, a carbon isotope effect k12/k13 = 1.0308 +/- 0.0006, and a carbon isotope effect for L-[alpha-2H]histidine of 1.0333 +/- 0.0001 at pH 6.3, 37 degrees C. These results indic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00416a016
更新日期:1988-08-09 00:00:00
abstract::A pterin analogue, 5-[(3-azido-6-nitrobenzylidene)amino]-2,6-diamino-4-pyrimidinone (ANBADP), was synthesized as a probe of the pterin binding site of phenylalanine hydroxylase. The photoaffinity label has been found to be a competitive inhibitor of the enzyme with respect to 6,7-dimethyltetrahydropterin, having a Ki ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00241a024
更新日期:1991-07-09 00:00:00
abstract::Deposition of misfolded alpha-synuclein is a critical factor in several neurodegenerative disorders. Filamentous alpha-synuclein is the major component of Lewy bodies and Lewy neurites, the intracellular inclusions in the dopaminergic neurons of the substantia nigra, which are considered the pathological hallmark of P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034938r
更新日期:2004-03-23 00:00:00
abstract::Segregation of mRNAs in the cytoplasm of polar cells has been demonstrated for proteins involved in Xenopus and Drosophila oogenesis, and for some proteins in somatic cells. It is assumed that vectorial transport of the messages is generally responsible for this localization. The mRNA encoding the basic protein of cen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9800247
更新日期:1998-05-12 00:00:00
abstract::Excitation energy transfer studies were carried out to determine the distance between the adenosinetriphosphatase (ATPase) site and a unique "fast-reacting" sulfhydryl (referred to as SH1) in myosin subfragment 1. The fluorescent moiety of the probe N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylene-diamine was used as th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00520a035
更新日期:1981-08-18 00:00:00
abstract::Resonance energy-transfer approaches have been used to directly monitor the interactions of the GTP gamma S-bound alpha subunit of transducin (alpha T GTP gamma S) with the retinal cyclic GMP phosphodiesterase (PDE). The PDE was labeled with 5-(iodoacetamido) fluorescein (IAF-PDE) and served as the fluorescence donor ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00243a011
更新日期:1991-07-23 00:00:00
abstract::The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00101a003
更新日期:1991-09-17 00:00:00
abstract::BtuB is a TonB-dependent transport protein that binds and carries vitamin B(12) across the outer membrane of Gram negative bacteria such as Escherichia coli. Previous work has demonstrated that the Ton box, a highly conserved segment near the N-terminus of the protein, undergoes an order-to-disorder transition upon th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061051x
更新日期:2006-09-12 00:00:00
abstract::The Sup35 protein of Saccharomyces cerevisiae forms a prion that generates the [PSI(+)] phenotype. Its NM region governs prion status, forming self-seeding amyloid fibers in vivo and in vitro. A tryptophan mutant of Sup35 (NM(F117W)) was used to probe its aggregation. Four indicators of aggregation, Trp 117 maximum em...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9000276
更新日期:2009-07-28 00:00:00
abstract::Transport of hydrophilic di- and tripeptides into Lactococcus lactis is mediated by a proton motive force-driven peptide transport protein (DtpT) that shares similarity with eukaryotic peptide transporters, e.g., from kidney and small intestine of rabbit, man, and rat. Hydropathy profiling in combination with the "pos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963068t
更新日期:1997-06-03 00:00:00
abstract::Annexin V is a Ca(2+)-dependent, phospholipid-binding protein that may have one or more membrane-related functions. The binding of annexin V to phospholipids in a detergent micelle matrix was studied to attempt to determine directly the stoichiometry of specific phospholipid-binding sites and the importance of negativ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00094a030
更新日期:1993-11-02 00:00:00
abstract::In vitro transcription by T7 RNA polymerase was used to prepare 32 different mutations in the 21 nucleotides that participate in the 9 tertiary base pairs or triples of yeast tRNAPhe. The mutations were designed either to disrupt the tertiary interaction or to change the sequence without disrupting the structure by tr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00462a014
更新日期:1990-03-13 00:00:00
abstract::De novo purine biosynthesis proceeds by two divergent paths. In bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (AIR) is converted to 4-carboxy-AIR (CAIR) by two enzymes: N(5)-carboxy-AIR (N(5)-CAIR) synthetase (PurK) and N(5)-CAIR mutase (class I PurE). In animals, the conversion of AIR to CAIR requires...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi102033a
更新日期:2011-05-31 00:00:00
abstract::Prokaryotic and eukaryotic cytochrome c oxidases and several bacterial ubiquinol oxidases compose a superfamily of heme-copper oxidases. These enzymes are terminal components of aerobic respiratory chains, the principal energy-generating systems of aerobic organisms. Two such heme-copper oxidases are the aa3-type cyto...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00091a046
更新日期:1993-10-12 00:00:00
abstract::It has been suggested that the mechanism of alkaline phosphatase (AP) is associative, or triester-like, because phosphorothioate monoesters are hydrolyzed by AP approximately 10(2)-fold slower than phosphate monoesters. This "thio effect" is similar to that observed for the nonenzymatic hydrolysis of phosphate trieste...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000899x
更新日期:2000-08-08 00:00:00
abstract::Recent work has resulted in the development of potent inhibitors of oligosaccharyl transferase (OT), the enzyme that catalyzes the cotranslational glycosylation of asparagine [Hendrickson, T. L., Spencer, J. R., Kato, M., and Imperiali, B. (1996) J. Am. Chem. Soc. 118, 7636-7637; Kellenberger, C., Hendrickson, T. L., ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982740e
更新日期:1999-04-27 00:00:00
abstract::A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00218a034
更新日期:1991-01-29 00:00:00
abstract::The fluorescence decay of apoazurin derived from Pseudomonas aeruginosa is monoexponential. By this criterion the population of molecules of apoazurin is homogeneous. The emission anisotropy factor and the absorption anisotropy factor at the red edge of the absorption band assume similar values, showing that the trypt...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00680a018
更新日期:1975-05-06 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00350
更新日期:2017-07-25 00:00:00
abstract::Isolation and characterization of a novel radiation-induced product, i.e., the 8-hydroxyguanine residue, produced in deoxyribonucleic acid (DNA), 2'-deoxyguanosine, and 2'-deoxyguanosine 5'-monophosphate by gamma-irradiation in aqueous solution, are described. For this purpose, gamma-irradiated DNA was first hydrolyze...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00337a032
更新日期:1985-07-30 00:00:00
abstract::Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b6f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700748y
更新日期:2007-10-02 00:00:00
abstract::A hairpin structure contains two conformationally distinct domains: a double-helical stem with Watson-Crick base pairs and a single-stranded loop that connects the two arms of the stem. By extensive 1D and 2D 500-MHz 1H NMR studies in H2O and D2O, it has been demonstrated that the DNA oligomers d(CGCCGCAGC) and d(CGCC...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00398a027
更新日期:1987-12-01 00:00:00