Formation of an 8-hydroxyguanine moiety in deoxyribonucleic acid on gamma-irradiation in aqueous solution.

abstract：:Serum paraoxonase hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. High serum paraoxonase levels appear to protect against the neurotoxic effects of organophosphorus substrates of this enzyme [Costa et al. (1990) Toxicol. Appl. Pharmacol. 103, 66-76]. The amino acid sequence accounting f...

journal_title：Biochemistry

authors： Hassett C,Richter RJ,Humbert R,Chapline C,Crabb JW,Omiecinski CJ,Furlong CE

更新日期：1991-10-22 00:00:00

abstract：:Recent studies have shown that there can be large differences in the stability of double and triple helical nucleic acid complexes, depending on whether RNA or DNA strands are involved. These differences have been attributed to structural differences in the sugar-phosphate backbone of these two polymers. However, sinc...

journal_title：Biochemistry

authors： Wang S,Kool ET

更新日期：1995-03-28 00:00:00

abstract：:DNA photolyase from Escherichia coli contains FAD plus a partially characterized, second chromophore. In vivo, the flavin is fully reduced (FADH2), but oxidation to a stable, blue radical (FADH.) occurs during enzyme isolation. The second chromophore is irreversibly reduced by reaction of the enzyme with sodium borohy...

journal_title：Biochemistry

authors： Jorns MS,Wang B,Jordan SP

更新日期：1987-10-20 00:00:00

abstract：:Glucocorticoid (GC) hormones induce apoptosis in T-cell and pre-B-cell acute lymphoblastic leukemia (ALL) cells. Steroid-mediated apoptosis requires a threshold level of the glucocorticoid receptor (GR) protein, and increasing the intracellular GR levels in ALL cells would augment their hormone sensitivity. A protein ...

journal_title：Biochemistry

authors： Geng CD,Vedeckis WV

更新日期：2010-10-19 00:00:00

abstract：:The electron spin echo decay envelope for the blue copper protein, stellacyanin, and for a number of other Cu(II) complexes has been studied. Particular attention was given to the form of the "nuclear modulation" patterns, which show the effects of coupling between the electron spin and the neighboring nuclei. The env...

journal_title：Biochemistry

authors： Mims WB,Peisach J

更新日期：1976-08-24 00:00:00

abstract：:The structure and backbone dynamics of an extended second transmembrane segment (TM2e) of the human neuronal glycine receptor alpha(1) subunit in sodium dodecyl sulfate micelles were studied by (1)H and (15)N solution-state NMR. The 28-amino acid segment contained the consensus TM2 domain plus part of the linker betwe...

journal_title：Biochemistry

authors： Yushmanov VE,Mandal PK,Liu Z,Tang P,Xu Y

更新日期：2003-04-08 00:00:00

abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...

journal_title：Biochemistry

authors： Santoro MM,Bolen DW

更新日期：1992-05-26 00:00:00

abstract：:We have used frequency domain fluorescence techniques to resolve the component emission spectra for several two tryptophan containing proteins (e.g., horse liver alcohol dehydrogenase, sperm whale apomyoglobin, yeast 3-phosphoglycerate kinase, apoazurin from Alcaligenes denitricans). We have first performed multifrequ...

journal_title：Biochemistry

authors： Eftink MR,Wasylewski Z,Ghiron CA

更新日期：1987-12-15 00:00:00

abstract：:Biotin synthase (BioB) is an iron-sulfur dimeric enzyme which catalyzes the last step in biotin synthesis. The reaction consists of the introduction of a sulfur atom into dethiobiotin. It is shown here that BioB displays a significant cysteine desulfurase activity, providing it with the ability to mobilize sulfur from...

journal_title：Biochemistry

authors： Ollagnier-De-Choudens S,Mulliez E,Hewitson KS,Fontecave M

更新日期：2002-07-23 00:00:00

abstract：:The kinetic parameters (Km and Vmax) of sugar-modified analogues of inosine and guanosine have been determined with human erythrocytic purine nucleoside phosphorylase (PNP). Steric alterations at the 2' and 3' positions greatly lessened or abolished substrate activity. However, the 5'-deoxy- and 2',5'-dideoxy-beta-D-r...

journal_title：Biochemistry

authors： Stoeckler JD,Cambor C,Parks RE Jr

更新日期：1980-01-08 00:00:00

abstract：:Reaction center (RC) complexes isolated from a Zn-bacteriochlorophyll (BChl) a-containing purple bacterium, Acidiphilium rubrum, were characterized by absorption, circular dichroism, and magnetic circular dichroism (MCD) spectroscopy. The oxidized-minus-reduced difference spectra indicated that, in this RC, the Zn-BCh...

journal_title：Biochemistry

authors： Mimuro M,Kobayashi M,Shimada K,Uezono K,Nozawa T

更新日期：2000-04-11 00:00:00

abstract：:Hepatic mixed-function oxidase metabolism of the ubiquitous pollutant polychlorinated biphenyls (PCBs) is implicated in their toxification and detoxification. We used dichlorobiphenyls (DCBs) as models to investigate the effect of the chloro substituent sites on this metabolism experimentally and by molecular orbital ...

journal_title：Biochemistry

authors： Kaminsky LS,Kennedy MW,Adams SM,Guengerich FP

更新日期：1981-12-22 00:00:00

abstract：:Glycosaminoglycans (GAGs) have been reported to play a significant role in amyloid formation of a wide range of proteins/peptides either associated with diseases or native biological functions. The exact mechanism by which GAGs influence amyloid formation is not clearly understood. Here, we studied two closely related...

journal_title：Biochemistry

authors： Jha NN,Anoop A,Ranganathan S,Mohite GM,Padinhateeri R,Maji SK

更新日期：2013-12-10 00:00:00

abstract：:Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...

journal_title：Biochemistry

authors： Thielges MC,Chung JK,Axup JY,Fayer MD

更新日期：2011-06-28 00:00:00

abstract：:The primary subsite specificities of human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II, bovine chymotrypsin A alpha, and the protease from strain V-8 of Staphylococcus aureus have been mapped with a series of tripeptide thiobenzyl ester substrates of the general formu...

journal_title：Biochemistry

authors： Harper JW,Cook RR,Roberts CJ,McLaughlin BJ,Powers JC

更新日期：1984-06-19 00:00:00

abstract：:A strategy to design potent antagonists of human coagulation factor VIIa (FVIIa) by linking two proteins that independently inhibit activity and bind at separate, nonoverlapping sites is presented. A bifunctional inhibitor (KDTF5), comprising a Kunitz-type domain engineered to inhibit the FVIIa active site and a solub...

journal_title：Biochemistry

authors： Lee GF,Lazarus RA,Kelley RF

更新日期：1997-05-13 00:00:00

abstract：:The membrane-bound quinoprotein glucose dehydrogenase (mGDH) in Escherichia coli contains pyrroloquinoline quinone (PQQ) and participates in the direct oxidation of D-glucose to D-gluconate by transferring electrons to ubiquinone (UQ). To elucidate the mechanism of ubiquinone reduction by mGDH, we applied a pulse radi...

journal_title：Biochemistry

authors： Kobayashi K,Mustafa G,Tagawa S,Yamada M

更新日期：2005-10-18 00:00:00

abstract：:Proteolytic activation, oligomerization, and membrane insertion are three steps that precede channel formation by the bacterial toxin aerolysin. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and hydrogen-deuterium exchange, the structural changes associated with each step were an...

journal_title：Biochemistry

authors： Cabiaux V,Buckley JT,Wattiez R,Ruysschaert JM,Parker MW,van der Goot FG

更新日期：1997-12-09 00:00:00

abstract：:The cardiac-specific Nkx2.5 homeodomain has been expressed as a 79-residue protein with the oxidizable Cys(56) replaced with Ser. The Nkx2.5 or Nkx2.5(C56S) homeodomain is 73% identical in sequence to and has the same NMR structure as the vnd (ventral nervous system defective)/NK-2 homeodomain of Drosophila when bound...

journal_title：Biochemistry

authors： Fodor E,Mack JW,Maeng JS,Ju JH,Lee HS,Gruschus JM,Ferretti JA,Ginsburg A

更新日期：2005-09-20 00:00:00

abstract：:The thermotropic phase behavior of 10 mixed-chain phosphatidylcholines, in excess water, has been examined and compared with that of identical-chain C(16):C(16)PC by using high-resolution differential scanning calorimetry (DSC). The molecular weights (MW) of these 11 molecular species are the same, but their delta C/C...

journal_title：Biochemistry

authors： Bultmann T,Lin HN,Wang ZQ,Huang CH

更新日期：1991-07-23 00:00:00

abstract：:Illumination of envelope vesicles prepared from Halobacterium halobium cells causes translocation of protons from inside to outside, due to the light-induced cycling of bacteriorhodopsin. This process results in a pH gradient across the membranes, an electrical potential, and the movements of K+ and Na+. The electrica...

journal_title：Biochemistry

authors： Renthal R,Lanyi JK

更新日期：1976-05-18 00:00:00

abstract：:The structure of an analogue of the yeast tRNAPhe T Psi C stem-loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified T Psi C stem-loop is the product of the m...

journal_title：Biochemistry

authors： Koshlap KM,Guenther R,Sochacka E,Malkiewicz A,Agris PF

更新日期：1999-07-06 00:00:00

abstract：:The structure of the adduct formed by reaction of acetaldehyde and Met5-enkephalin has been determined by analysis of 400-MHz proton spectra: two-dimensional J spectroscopy was used to resolve and measure virtually all the overlapping resonances, and decoupling difference spectroscopy was used to assign the resonances...

journal_title：Biochemistry

authors： Gidley MJ,Hall LD,Sanders JK,Summers MC

更新日期：1981-06-23 00:00:00

abstract：:A specific requirement for lipids, particularly cardiolipin (CL), in cytochrome c oxidase (CcO) has been reported in many previous studies using mainly in vitro lipid removal approaches in mammalian systems. Our accompanying paper shows that CcO produced in markedly CL-depleted Rhodobacter sphaeroides displays wild-ty...

journal_title：Biochemistry

authors： Zhang X,Hiser C,Tamot B,Benning C,Reid GE,Ferguson-Miller SM

更新日期：2011-05-17 00:00:00

abstract：:Hemocyanin (Hc) is a dinuclear copper protein that binds oxygen reversibly. The structure of the Cu(II) site in a derivative of hemocyanin known as green half-met (GHM) has been analyzed using the pulsed EPR technique of electron spin-echo envelope modulation (ESEEM) spectroscopy. The derivative, prepared by treating ...

journal_title：Biochemistry

authors： Magliozzo RS,Bubacco L,McCracken J,Jiang F,Beltramini M,Salvato B,Peisach J

更新日期：1995-02-07 00:00:00

abstract：:GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...

journal_title：Biochemistry

authors： Syson K,Stevenson CE,Rashid AM,Saalbach G,Tang M,Tuukkanen A,Svergun DI,Withers SG,Lawson DM,Bornemann S

更新日期：2014-04-22 00:00:00

abstract：:Three activities on the pathway of purine biosynthesis de novo in chicken liver, namely, glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase, have been found to reside on the same polypeptide chain. Three diverse purification schemes, utilizing...

journal_title：Biochemistry

authors： Daubner SC,Schrimsher JL,Schendel FJ,Young M,Henikoff S,Patterson D,Stubbe J,Benkovic SJ

更新日期：1985-12-03 00:00:00

abstract：:Ketosteroid isomerase (KSI) from Pseudomonas putida biotype B is a homodimeric enzyme catalyzing an allylic rearrangement of Delta5-3-ketosteroids at rates comparable with the diffusion-controlled limit. The tyrosine triad (Tyr14.Tyr55.Tyr30) forming a hydrogen-bond network in the apolar active site of KSI has been ch...

journal_title：Biochemistry

authors： Nam GH,Jang DS,Cha SS,Lee TH,Kim DH,Hong BH,Yun YS,Oh BH,Choi KY

更新日期：2001-11-13 00:00:00

abstract：:The newly discovered oncogenic protein gankyrin, which contains six ankyrin repeats, has been reported to be involved in the phosphorylation and degradation of the retinoblastoma gene product, Rb. Using in vitro systems, we have identified a peptide fragment of gankyrin, 176LHLACDEERN185, which is responsible for bind...

journal_title：Biochemistry

authors： Li J,Tsai MD

更新日期：2002-03-26 00:00:00

abstract：:Translational initiation factor IF-2 is involved in a multistep pathway leading to the synthesis of the first peptide bond. IF-2 is a guanine nucleotide binding protein (G-protein) and catalyzes GTP hydrolysis in the presence of ribosomes. According to sequence homologies with other G-proteins, particularly EF-Tu, a t...

journal_title：Biochemistry

authors： Vachon G,Laalami S,Grunberg-Manago M,Julien R,Cenatiempo Y

更新日期：1990-10-16 00:00:00