Abstract:
:The electron spin echo decay envelope for the blue copper protein, stellacyanin, and for a number of other Cu(II) complexes has been studied. Particular attention was given to the form of the "nuclear modulation" patterns, which show the effects of coupling between the electron spin and the neighboring nuclei. The envelopes for the hydrated cupric complex and for copper(II) glycylglycine were essentially the same and indicative of the coupling to protons. The peptide complex contains nitrogen nuclei coupled directly to Cu(II), but the coupling constant is so large for these nuclei that a modulation pattern ascribable to 14N is not seen. For copper(II) bovine serum albumin, on the other hand, a contribution due to the coupling of the remote nitrogen belonging to a histidyl imidazole ligand was observed. The modulation pattern for this complex and for stellacyanin closely resembled one another, strongly suggesting that an imidazole is ligated to the copper in this blue protein.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Mims WB,Peisach Jdoi
10.1021/bi00662a033subject
Has Abstractpub_date
1976-08-24 00:00:00pages
3863-9issue
17eissn
0006-2960issn
1520-4995journal_volume
15pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Human acidic mammalian chitinase (AMCase), a member of the family 18 glycosyl hydrolases, is one of the important proteins involved in Th2-mediated inflammation and has been implicated in asthma and allergic diseases. Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a mo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0525977
更新日期:2006-04-11 00:00:00
abstract::The structures of ternary complexes of human inositol monophosphatase with inhibitory Gd3+ and either D- or L-myo-inositol 1-phosphate have been determined to 2.2-2.3 A resolution using X-ray crystallography. Substrate and metal are bound identically in each active site of the phosphatase dimer. The substrate is prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00198a011
更新日期:1994-08-16 00:00:00
abstract::The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00267a039
更新日期:1982-11-23 00:00:00
abstract::The fatty acid synthase (FAS) of animal tissue is a dimer of two identical subunits, each with a Mr of 260,000. The subunit is a single multifunctional protein having seven catalytic activities and a site for binding of the prosthetic group 4'-phosphopantetheine. The mRNA coding for the subunit has an estimated size o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00420a029
更新日期:1988-10-04 00:00:00
abstract::The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00559a031
更新日期:1980-09-02 00:00:00
abstract::Light-induced hypsochromic shifts of the Q(y) absorption band of the bacteriochlorophyll dimer (P) from 865 to 850 nm were identified using continuous illumination of dark-adapted reaction centers (RCs) from Rhodobacter capsulatus when dispersed in the most commonly used detergent, the zwitterionic lauryl N-dimethylam...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200595z
更新日期:2011-06-14 00:00:00
abstract::Tissue plasminogen activator-inhibitor complexes were purified from the conditioned medium of human umbilical vein endothelial cells by affinity chromatography followed by gel filtration. It was found that a single complex was isolated which can exist in two distinct interconvertible conformations. These may be separa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00397a037
更新日期:1987-11-17 00:00:00
abstract::The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901006b
更新日期:2009-08-18 00:00:00
abstract::A soluble Ca2+/calmodulin-dependent protein kinase has been purified from rat brain to near homogeneity by using casein as substrate. The enzyme was purified by using hydroxylapatite adsorption chromatography, phosphocellulose ion-exchange chromatography, Sepharose 6B gel filtration, affinity chromatography using calm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00318a018
更新日期:1984-11-06 00:00:00
abstract::By using some mutant peptides of transcription factor Spl, phosphate backbone contacts with the DNA binding protein containing three zinc fingers have been investigated by alkylation interference, circular permutation, DNase I footprinting, and methylation protection methods. The ethylation interference analyses of Sp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952530r
更新日期:1996-07-02 00:00:00
abstract::Escherichia coli helicase II (UvrD) protein can initiate unwinding of duplex DNA at blunt ends or nicks, although these reactions require excess protein. We have undertaken kinetic studies of these reactions in order to probe the mechanism of initiation of unwinding. DNA unwinding was monitored directly by using agaro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00066a039
更新日期:1993-04-20 00:00:00
abstract::To establish the state of protonation of quinonoid species formed nonenzymically from pyridoxal phosphate (PLP) and diethyl aminomalonate, we have studied absorption spectra of the rapidly established steady-state mixture of species. We have evaluated the formation constant and the spectrum of the mixture of Schiff ba...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00413a050
更新日期:1988-06-28 00:00:00
abstract::Nuclear Overhauser effect (NOE) measurements are reported for the nonexchangeable base and ribose 1' proton resonances of CCGGAp, ACCGGp, and ACCGGUp. The results permit assignment of these resonances to particular nucleotides in the sequences. The NOE data rule out conformations containing syn glycosidic linkages and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00271a005
更新日期:1983-01-18 00:00:00
abstract::Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in hig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00464a015
更新日期:1990-03-27 00:00:00
abstract::Myosin belongs to the family of motor proteins. Its interaction with actin coupled with hydrolysis of ATP is the molecular basis of muscle contraction. The head segment of myosin, called subfragment 1 (S1), contains the distinct binding sites for ATP and actin and responsible for the ATPase activity. The rate-limiting...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952565r
更新日期:1996-04-09 00:00:00
abstract::The Plasmodium falciparum malarial parasite genome appears to encode one and only one phosphatidylinositol 3'-kinase (PI3K), and sequence analysis suggests that the enzyme is a "class III"- or "Vps34"-type PI3K. PfVps34 has generated excitement as a possible druggable target and potentially a key target of artemisinin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00416
更新日期:2017-08-22 00:00:00
abstract::Known crystal structures of class II aminoacyl-tRNA synthetases complexed to their cognate tRNAs reveal that critical acceptor stem contacts are made by the variable loop connecting the beta-strands of motif 2 located within the catalytic core of class II synthetases. To identify potential acceptor stem contacts made ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001835p
更新日期:2000-12-19 00:00:00
abstract::The interaction of the 20-kDa pore-forming domain of colicin A with phospholipid vesicles was investigated by gel permeation chromatography, analytical centrifugation, and electron microscopy. Under the experimental conditions of this study, this peptide was found to interact only with vesicles containing negatively c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00445a029
更新日期:1989-09-19 00:00:00
abstract::While the iron-molybdenum cofactor (FeMoco) of nitrogenase, a constituent of the active site for nitrogen reduction, can be extracted into N-methylformamide (NMF) and pyrrollidinone, the inability to solubilize it in any other organic solvents has hampered further understanding of its structure and chemical properties...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00450a024
更新日期:1989-11-28 00:00:00
abstract::Purification and potential tachykinin and enkephalin precursor cleaving enzymes from bovine chromaffin granules was undertaken using as substrates the model precursors 35S-(Met)-beta-preprotachykinin [35S-(Met)-beta-PPT] and 35S-(Met)-preproenkephalin [35S-(Met)-PPE]. Purification by concanavalin A-Sepharose, Sephacry...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00132a011
更新日期:1992-05-05 00:00:00
abstract::The N-type voltage-gated Ca(2+) channel CaV2.2 is one of the important targets for pain management. ω-Conotoxins isolated from venoms of cone snails, which specifically inhibit CaV2.2, are promising scaffolds for novel analgesics. The inhibitory action of ω-conotoxins on CaV2.2 has been examined experimentally, but th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4003327
更新日期:2013-05-28 00:00:00
abstract::In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501160q
更新日期:2015-01-20 00:00:00
abstract::The acid-base properties of ionophore A23187 in methanol-water solutions (0--95% w/w) and bound to unilamellar vesicles of dimyristoylphosphatidylcholine were examined by ultraviolet and fluorescence spectroscopy, and the spectral properties for the acidic and basic forms were defined under these conditions. Standard ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00539a023
更新日期:1982-05-11 00:00:00
abstract::Diphtheria toxin (DT) has been studied as a model for understanding active-site structure and function in the ADP-ribosyltransferases. Earlier evidence suggested that histidine-21 of DT is important for the ADP-ribosylation of eukaryotic elongation factor 2 (EF-2). We have generated substitutions of this residue by ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00183a019
更新日期:1994-05-03 00:00:00
abstract::Thermodynamics of 39 oligonucleotides with internal G.T mismatches dissolved in 1 M NaCl were determined from UV absorbance versus temperature profiles. These data were combined with literature values of six sequences to derive parameters for 10 linearly independent trimer and tetramer sequences with G.T mismatches an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962590c
更新日期:1997-08-26 00:00:00
abstract::Heparin was isolated from an unusually large human hemangioma that contained an elevated level of mast cells. Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its structure and activity. Characterization of this human heparin included the following: one-d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00164a020
更新日期:1992-12-15 00:00:00
abstract::TOPORS is the first example of a protein that possesses both ubiquitin and SUMO E3 ligase activity. The ubiquitination activity maps to a conserved RING domain in the N-terminal region of the protein, which is not required for sumoylation activity. Similar to other E3 ligases, it is likely that the ubiquitin and sumoy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801904q
更新日期:2008-12-30 00:00:00
abstract::The kinetics of the phospholipase A2-catalyzed hydrolysis of bilayer vesicles and mixed micelles of several oxyglycero and thioglycero analogues of phospholipids have been studied. The results with vesicles show that, depending on the source of the enzyme, the rates of hydrolysis of the oxy-containing long-chain phosp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00149a014
更新日期:1992-09-01 00:00:00
abstract::The structural, electronic, and catalytic properties of cytochrome P450cam are subtly altered when the cysteine that coordinates to the heme iron is replaced with a selenocysteine. To map the effects of the sulfur-to-selenium substitution on the individual steps of the catalytic cycle, we conducted a comparative kinet...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00939
更新日期:2015-11-10 00:00:00
abstract::A member of the SF2 family of helicases, Escherichia coli RecQ, is involved in the recombination and repair of double-stranded DNA breaks and single-stranded DNA (ssDNA) gaps. Although the unwinding activity of this helicase has been studied biochemically, the mechanism of translocation remains unclear. To this end, u...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3000676
更新日期:2012-04-03 00:00:00