当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Conformational changes in aerolysin during the transition from the water-soluble protoxin to the membrane channel.

Conformational changes in aerolysin during the transition from the water-soluble protoxin to the membrane channel.

Abstract：

:Proteolytic activation, oligomerization, and membrane insertion are three steps that precede channel formation by the bacterial toxin aerolysin. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and hydrogen-deuterium exchange, the structural changes associated with each step were analyzed. Our results show that activation induces a significant change in secondary structure, characterized by a decrease in random structure and an increase in beta-sheet content. We show that release of the propeptide is essential for this conformational change to occur and that changes are not restricted to the vicinity of the cleavage site but appear to propagate along the molecule. In contrast, subsequent oligomerization of the mature toxin does not involve any change in overall secondary structure but does involve a modification of the tertiary interactions. Finally, insertion of the heptameric complex into dimyristoylphosphatidylcholine vesicles also occurs without major modification of the secondary structure. Studies on the orientations of the secondary structures of the heptamer in the lipid bilayer have also been performed.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Cabiaux V,Buckley JT,Wattiez R,Ruysschaert JM,Parker MW,van der Goot FG

doi

10.1021/bi971216p

subject

Has Abstract

pub_date

1997-12-09 00:00:00

pages

15224-32

issue

49

eissn

0006-2960

issn

1520-4995

pii

bi971216p

journal_volume

36

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Pulse radiolysis studies on cytochrome cd1 nitrite reductase from Thiosphaera pantotropha: evidence for a fast intramolecular electron transfer from c-heme to d1-heme.

    abstract：:Electron transfer within cytochrome cd1 from Thiosphaera pantotropha was investigated by the technique of pulse radiolysis. The reduction of the heme centers in this nitrite reductase occurred in two phases as judged from kinetic difference spectra. In the faster phase, radiolytically generated N-methylnicotinamide (N...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971045o

    authors： Kobayashi K,Koppenhöfer A,Ferguson SJ,Tagawa S

    更新日期：1997-11-04 00:00:00

  • Primer length dependence of binding of DNA polymerase I Klenow fragment to template-primer complexes containing site-specific bulky lesions.

    abstract：:The binding of the benzo[a]pyrene metabolite anti-BPDE (r7, t8-dihydroxy-t9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) to the N(2) group of 2'-deoxyguanosine residues (dG) is known to adversely affect the Michaelis-Menten primer extension kinetics catalyzed by DNA Pol I and other polymerases. In this work, the impact...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990614k

    authors： Rechkoblit O,Amin S,Geacintov NE

    更新日期：1999-09-07 00:00:00

  • An endogenous sulfated inhibitor of neuronal inositol trisphosphate receptors.

    abstract：:In cerebellum, inositol trisphosphate- (InsP(3)-) gated Ca channels play a key role in learning, though they exhibit a low sensitivity to InsP(3) compared to peripheral tissues. In the present study, the cerebellar InsP(3) receptor is shown to be associated with a novel inhibitor of InsP(3) binding. (3)H-InsP(3) bindi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992175v

    authors： Watras J,Orlando R,Moraru II

    更新日期：2000-03-28 00:00:00

  • GRP1 pleckstrin homology domain: activation parameters and novel search mechanism for rare target lipid.

    abstract：:Pleckstrin homology (PH) domains play a central role in a wide array of signaling pathways by binding second messenger lipids of the phosphatidylinositol phosphate (PIP) lipid family. A given type of PIP lipid is formed in a specific cellular membrane where it is generally a minor component of the bulk lipid mixture. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049017a

    authors： Corbin JA,Dirkx RA,Falke JJ

    更新日期：2004-12-28 00:00:00

  • Solid-state NMR investigation of the dynamics of the soluble and membrane-bound colicin Ia channel-forming domain.

    abstract：:Solid-state NMR spectroscopy was employed to study the molecular dynamics of the colicin Ia channel domain in the soluble and membrane-bound states. In the soluble state, the protein executes small-amplitude librations (with root-mean-square angular fluctuations of 0-10 degrees ) in the backbone and larger-amplitude m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0027231

    authors： Huster D,Xiao L,Hong M

    更新日期：2001-06-26 00:00:00

  • Steps toward constructing a cytochrome b6 f complex in the purple bacterium Rhodobacter sphaeroides: an example of the structural plasticity of a membrane cytochrome.

    abstract：:We have modified the cytochrome b subunit of the cytochrome bc1 complex from the purple bacterium Rhodobacter sphaeroides to introduce two distinctive features of cytochrome b6 f complexes. In the first one, we have split cyt b into two polypeptides thus mimicking the organization of cyt b6 and subunit IV in the b6 f ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9813476

    authors： Kuras R,Guergova-Kuras M,Crofts AR

    更新日期：1998-11-17 00:00:00

  • Substrate activation by acyl-CoA dehydrogenases: transition-state stabilization and pKs of involved functional groups.

    abstract：:The mechanism by which acyl-CoA dehydrogenases initiate catalysis was studied by using p-substituted phenylacetyl-CoAs (substituents-NO2, -CN, and CH3CO-), 3S-C8-, and 3'-dephospho-3S-C8CoA. These analogues lack a beta C-H and cannot undergo alpha,beta-dehydrogenation. Instead they deprotonate at alpha C-H at pH > or ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971827h

    authors： Vock P,Engst S,Eder M,Ghisla S

    更新日期：1998-02-17 00:00:00

  • Functional analysis of amino acid residues essential for activity in the Na+/H+ exchanger of fission yeast.

    abstract：:We identified amino acid residues important for activity of sod2, the Na+/H+ antiporter of Schizosaccharomyces pombe. We mutated all eight His residues of sod2 into Arg. Only His367-->Arg affected function and resulted in complete inability of sod2 to allow growth of S. pombe in LiCl-containing medium. Mutant S. pombe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9801457

    authors： Dibrov P,Young PG,Fliegel L

    更新日期：1998-06-09 00:00:00

  • An additional copy of the homologous region (hr1) sequence in the Autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes.

    abstract：:The Autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. Our results show that the 750 bp hr1 sequence is capable ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049953q

    authors： Venkaiah B,Viswanathan P,Habib S,Hasnain SE

    更新日期：2004-06-29 00:00:00

  • Uracil DNA-glycosylase/glyceraldehyde-3-phosphate dehydrogenase is an Ap4A binding protein.

    abstract：:A 37 kDa protein that binds to diadenosine tetraphosphate (Ap4A) was purified from human HeLa cells and identified as uracil DNA glycosylase/glyceraldehyde-3-phosphate dehydrogenase (UDG/GAPDH). Utilizing photoaffinity labeling with [alpha-32P]8N3-Ap4A, an Ap4A binding protein of 37 kDa was identified from HeLa cell n...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00030a007

    authors： Baxi MD,Vishwanatha JK

    更新日期：1995-08-01 00:00:00

  • Biophysical characterization of the c-Myb DNA-binding domain.

    abstract：:We have examined proteins containing the DNA-binding domain of c-Myb with biophysical methods. This DNA-binding domain consists of three imperfect repeats (R1, R2, and R3) conserved among many species. Our results indicate that the DNA-binding domain forms unspecific and specific complexes with oligodeoxynucleotides. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00252a026

    authors： Ebneth A,Schweers O,Thole H,Fagin U,Urbanke C,Maass G,Wolfes H

    更新日期：1994-12-06 00:00:00

  • Conformational transition required for efficient splicing of transcripts from hybrid lambda promoter yeast tRNA gene fusion.

    abstract：:Fusion of a prokaryotic promoter to a yeast tRNA gene provides a means for uncoupling analyses of mutations affecting splicing from requirements for transcription and other processing steps. For this purpose, a phage lambda promoter was fused to the Saccharomyces cerevisiae tRNATyr(SUP3a) coding sequence. This fusion ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00240a017

    authors： Shapero MH,Greer CL

    更新日期：1991-07-02 00:00:00

  • Expression and purification of the extracellular ligand-binding domain of the atrial natriuretic peptide (ANP) receptor: monovalent binding with ANP induces 2:2 complexes.

    abstract：:The receptor for atrial natriuretic peptide (ANP) is a type-I transmembrane protein containing an extracellular ligand-binding domain, a single transmembrane sequence, an intracellular kinase-homologous domain, and a guanylate cyclase (GCase) domain. Binding of ANP to the extracellular domain causes activation of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982127v

    authors： Misono KS,Sivasubramanian N,Berkner K,Zhang X

    更新日期：1999-01-12 00:00:00

  • Crystal structure of a prolonged-acting insulin with albumin-binding properties.

    abstract：:The fatty acid acylated insulin, Lys(B29)-tetradecanoyl, des-(B30) human insulin, has been crystallized and the structure determined by X-ray crystallography. The fatty acid substituent on residue B29 Lys binds reversibly to circulating albumin protein in vivo, and by this mechanism the hormone's action is prolonged. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9625105

    authors： Whittingham JL,Havelund S,Jonassen I

    更新日期：1997-03-11 00:00:00

  • Thiophilic adsorption: a comparison of model protein behavior.

    abstract：:A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00396a049

    authors： Hutchens TW,Porath J

    更新日期：1987-11-03 00:00:00

  • Observations on the pyridoxal 5'-phosphate inhibition of DNA polymerases.

    abstract：:Pyridoxal 5'-phosphate at concentrations greater than 0.5 mM inhibits polymerization of deoxynucleoside triphosphate catalyzed by a variety of DNA polymerases. The requirement for a phosphate as well as aldehyde moiety of pyridoxal phosphate for inhibition to occur is clearly shown by the fact that neither pyridoxal n...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00661a033

    authors： Modak MJ

    更新日期：1976-08-10 00:00:00

  • Amplitude analysis of single-wavelength time-dependent absorption data does not support the conventional sequential mechanism for the reduction of dioxygen to water catalyzed by bovine heart cytochrome c oxidase.

    abstract：:The reactions of fully reduced and mixed-valence bovine heart cytochrome c oxidase with dioxygen have been reinvestigated in the absence and presence of metal ions (Zn(2+), Ni(2+), and Cd(2+)) by time-resolved optical absorption spectroscopy using the CO flow-flash technique. The time-resolved data were recorded on a ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049408p

    authors： Szundi I,Cappuccio J,Einarsdóttir O

    更新日期：2004-12-21 00:00:00

  • Characterization of a cloned human dihydrotestosterone/androstanediol UDP-glucuronosyltransferase and its comparison to other steroid isoforms.

    abstract：:A human cDNA, UDPGTh-3, encoding a dihydrotestosterone/5 alpha-androstane-3 alpha,17 beta-diol UDP- glucuronosyltransferase (transferase) has been isolated and characterized. The nucleotide sequence of UDPGTh-3 encodes a 530 amino acid protein with a typical membrane insertion-signal peptide, a membrane-anchoring doma...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00091a015

    authors： Chen F,Ritter JK,Wang MG,McBride OW,Lubet RA,Owens IS

    更新日期：1993-10-12 00:00:00

  • Disrupted zinc-binding sites in structures of pathogenic SOD1 variants D124V and H80R.

    abstract：:Mutations in human copper-zinc superoxide dismutase (SOD1) cause an inherited form of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS). Here, we present structures of the pathogenic SOD1 variants D124V and H80R, both of which demonstrate compromised zinc-binding sites. The disruption of the zinc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100314n

    authors： Seetharaman SV,Winkler DD,Taylor AB,Cao X,Whitson LJ,Doucette PA,Valentine JS,Schirf V,Demeler B,Carroll MC,Culotta VC,Hart PJ

    更新日期：2010-07-13 00:00:00

  • Activity and crystal structure of Arabidopsis thaliana UDP-N-acetylglucosamine acyltransferase.

    abstract：:The UDP-N-acetylglucosamine (UDP-GlcNAc) acyltransferase, encoded by lpxA, catalyzes the first step of lipid A biosynthesis in Gram-negative bacteria, the (R)-3-hydroxyacyl-ACP-dependent acylation of the 3-OH group of UDP-GlcNAc. Recently, we demonstrated that the Arabidopsis thaliana orthologs of six enzymes of the b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi3002242

    authors： Joo SH,Chung HS,Raetz CR,Garrett TA

    更新日期：2012-05-29 00:00:00

  • Modular mutagenesis of exons 1, 2, and 8 of a glutathione S-transferase from the mu class. Mechanistic and structural consequences for chimeras of isoenzyme 3-3.

    abstract：:Exons 1 and 2 and exon 8 of the mu class GSH transferases from rat encode sequence-variable regions 1 and 4 of mu class isoenzymes, respectively. These two of four variable regions are located at the N- and C-termini of this isoenzyme class and impinge on the active site. In order to assess the influence of these vari...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00157a005

    authors： Zhang P,Liu S,Shan SO,Ji X,Gilliland GL,Armstrong RN

    更新日期：1992-10-27 00:00:00

  • Self-assembly of designed antimicrobial peptides in solution and micelles.

    abstract：:Hydrophobic interactions are responsible for stabilizing leucine zippers in peptides containing heptad repeats. The effects of substituting leucine by phenylalanine and alanine by glycine on the self-assembly of coiled-coils were examined in minimalist antimicrobial peptides designed to form amphipathic alpha-helices....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961644f

    authors： Javadpour MM,Barkley MD

    更新日期：1997-08-05 00:00:00

  • Structural changes in the peptide backbone in complex formation between activated rhodopsin and transducin studied by FTIR spectroscopy.

    abstract：:Structural changes in the complex formation between transducin and metarhodopsin II, the activated form of photolyzed rhodopsin, in visual transduction processes were analyzed by Fourier transform infrared spectroscopy. The spectrum of the complex was obtained by subtracting the contribution of metarhodopsin I and unc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960911e

    authors： Nishimura S,Sasaki J,Kandori H,Matsuda T,Fukada Y,Maeda A

    更新日期：1996-10-15 00:00:00

  • Enzymatic activation of DNA cleavage by dynemicin A and synthetic analogs.

    abstract：:Dynemicin A (1), a member of the enediyne family of natural products, binds to double-stranded DNA (K(B) approximately 10(4) M(-1)) and in the presence of millimolar concentrations of a reducing cofactor such as NADPH or GSH reacts to cleave DNA. In this work, we show that the two flavin-based enzymes ferredoxin-NADP+...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962976n

    authors： Myers AG,Kort ME,Cohen SB,Tom NJ

    更新日期：1997-04-01 00:00:00

  • New synthetic cluster ligands for galactose/N-acetylgalactosamine-specific lectin of mammalian liver.

    abstract：:Synthetic ligands containing up to six residues of nonreducing terminal galactose were prepared. The synthesis involved coupling of carboxyl groups of N-benzyloxy-carbonylaspartic acid or of N-benzyloxycarbonyltyrosyl-gamma-glutamylglutamic acid to the omega-amino group of the aglycon of a glycoside that contained up ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00313a037

    authors： Lee RT,Lin P,Lee YC

    更新日期：1984-08-28 00:00:00

  • Conserved rhodopsin intradiscal structural motifs mediate stabilization: effects of zinc.

    abstract：:Retinitis pigmentosa (RP), a neurodegenerative disorder, can arise from single point mutations in rhodopsin, leading to a cascade of protein instability, misfolding, aggregation, rod cell death, retinal degeneration, and ultimately blindness. Divalent cations, such as zinc and copper, have allosteric effects on misfol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800968w

    authors： Gleim S,Stojanovic A,Arehart E,Byington D,Hwa J

    更新日期：2009-03-03 00:00:00

  • Chimeric human calcitonin and glucagon receptors reveal two dissociable calcitonin interaction sites.

    abstract：:Two chimeric receptors were constructed by transposing the coding regions for the putative N-terminal domains of the human calcitonin (hCTR) and glucagon (hGGR) receptors. These receptors were stably expressed as glycosylated proteins with molecular masses of 80 kDa for the calcitonin receptor N-terminus chimera (NtCT...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00003a040

    authors： Stroop SD,Kuestner RE,Serwold TF,Chen L,Moore EE

    更新日期：1995-01-24 00:00:00

  • Orientation-conserved transfer of symmetric Krebs cycle intermediates in mammalian tissue.

    abstract：:Metabolism of [2-13C]-, [3-13C]-, and [1,2,3-13C]propionate in perfused rat livers and [2-13C]-acetate in perfused rat hearts has been examined in tissue extracts by 13C NMR. Label from [2-13C]-propionate was preferentially incorporated into the C2 carbon of lactate, alanine, and aspartate in liver tissue while label ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00186a029

    authors： Sherry AD,Sumegi B,Miller B,Cottam GL,Gavva S,Jones JG,Malloy CR

    更新日期：1994-05-24 00:00:00

  • Estimating the degree of expansion in the transition state for protein unfolding: analysis of the pH dependence of the rate constant for caricain denaturation.

    abstract：:The thermal denaturation of caricain (the most alkaline of papain-related proteinases) was studied in acid media. Under all conditions tested, caricain denatured irreversibly following a single first-order reaction that involves simultaneous loss of secondary and tertiary structures. Besides, variation of the rate con...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991658w

    authors： López-Arenas L,Solís-Mendiola S,Hernández-Arana A

    更新日期：1999-11-30 00:00:00

  • Analysis of amino and carboxy terminal GLUT-4 targeting motifs in 3T3-L1 adipocytes using an endosomal ablation technique.

    abstract：:The targeting of the insulin-responsive glucose transporter, GLUT-4, to an intracellular compartment in adipocytes and muscle is one of the key features responsible for the unique insulin sensitivity of this transporter. Through expression of epitope-tagged GLUT-4 mutants in 3T3-L1 adipocytes, two motifs have been ide...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980988y

    authors： Melvin DR,Marsh BJ,Walmsley AR,James DE,Gould GW

    更新日期：1999-02-02 00:00:00