Abstract:
:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogenous DNA was (A + T)-richer than the DNA in chromatin, and when the mixture was exposed to 0.6 M NaCl or formaldehyde. When a (G + C)-richer DNA was added as the acceptor for histones, no substantial transfer of histones from chromatin DNA to exogenous DNA was found. Thus the activation energy of histone dissociation from chromatin DNA seems to be substantially lowered by 0.6 M NaCl or formaldehyde such that histones (mostly histone Hl) can be dissociated and bind the (A + T)-richer DNA and form a more stable complex. It is suggested that the formaldehyde effect on histones may be due to the loss of positive charges on lysine and arginin residues (probably more on lysine than on arginine) in histones after their rapid reaction with formaldehyde. Formaldehyde treatment of chromatin also distorts the DNA conformation, as revealed by circular dichroism (CD) studies. This structural effect occurs mainly on those base pairs bound by histones other than Hl, or within the chromatin subunit. Histone redistribution is treated as a thermodynamic phenomenon of histone binding to DNA. The validity of using formaldehyde to study chromatin structure is discussed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Polacow I,Cabasso L,Li HJdoi
10.1021/bi00666a002subject
Has Abstractpub_date
1976-10-19 00:00:00pages
4559-65issue
21eissn
0006-2960issn
1520-4995journal_volume
15pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The membrane-proximal external region (MPER) of the gp41 fusion protein of HIV is highly conserved among isolates of this virus and is considered a target for vaccine development. This region also appears to play a role in membrane fusion as well as localization of the virus to cholesterol-rich domains in membranes. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8014828
更新日期:2008-11-11 00:00:00
abstract::The molecular structure of the T4 phage tail sheath protein, gp18, was studied by limited proteolysis, immunoblotting, and immunoelectron microscopy. Gp18 is extremely resistant to proteolysis in the assembled form of either extended or contracted sheaths, but it is readily cleaved by proteases in the monomeric form, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00473a009
更新日期:1990-05-29 00:00:00
abstract::Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2015822
更新日期:2011-12-20 00:00:00
abstract::Sulfhydryl-reactive derivatives of glucosamine were synthesized as potentially transportable affinity labels of the human erythrocyte hexose carrier. N-Maleoylglycyl derivatives of either 6- or 2-amino-2-deoxy-D-glucopyranose were the most potent inhibitors of 3-O-methylglucose uptake, with concentrations of half-maxi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00430a044
更新日期:1989-02-21 00:00:00
abstract::Decarboxylated S-adenosylmethionine was found to be a substrate for the nuclear acetyltransferases that act on polyamines and on histones. The rate of acetylation of decarboxylated S-adenosylmethionine was more than twice that of spermidine at saturating substrate concentrations, and decarboxylated S-adenosylmethionin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00350a016
更新日期:1986-01-28 00:00:00
abstract::Understanding the molecular mechanisms of enzyme catalysis and allosteric regulation has been a primary goal of biochemistry for many years. The dynamics of these processes, approached through a variety of kinetic methods, are discussed. The results obtained for many different enzymes suggest that multiple intermediat...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi0260839
更新日期:2002-07-02 00:00:00
abstract::The dietary flavonoid quercetin has a broad range of biological activities, including potent antitumor activity against several types of tumors. Recently, it has been shown that quercetin inhibits cancer cells proliferation by depleting cellular microtubules and perturbing cellular microtubule functions. However, the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025952r
更新日期:2002-10-29 00:00:00
abstract::Methylmalonyl-CoA mutase from Propionibacterium shermanii is an adenosylcobalamin-dependent enzyme which catalyzes the reversible isomerization of methylmalonyl-CoA and succinyl-CoA. The rate of tritium loss from 5'-[3H]adenosylcobalamin during the enzymic reaction and the relative rates of tritium appearance in subst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961250o
更新日期:1996-09-10 00:00:00
abstract::Vesicular stomatitis virus buds from domains of the plasma membrane that have a unique protein and lipid composition. Fluorescence digital imaging microscopy and resonance energy transfer were used to determine how the two viral envelope-associated proteins, the G and the M proteins, could alter the lateral distributi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00031a008
更新日期:1995-08-08 00:00:00
abstract::The bond cleavage step of pepsinogen activation has been investigated in a kinetic study in which the denatured products of short-term acidifications were separated on SDS-polyacrylamide gels and the peptide products were quantitated by densitometry. Although several peptide products were observed, under the condition...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00432a040
更新日期:1989-03-21 00:00:00
abstract::A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a042
更新日期:1977-04-05 00:00:00
abstract::The equilibrium binding of influenza virus hemagglutinin to derivatives of its cell-surface ligand, sialic acid, was measured by nuclear magnetic resonance (NMR) spectroscopy. Binding was quantified by observing perturbations of sialic acid resonances in the presence of protein. The major perturbation observed was a c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00447a018
更新日期:1989-10-17 00:00:00
abstract::Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01242
更新日期:2018-03-13 00:00:00
abstract::Corticosteroid binding globulin (CBG), a serum glycoprotein which binds glucocorticoids and progestins with high affinity, is widely distributed throughout the animal world. Although its charge and size characteristics have largely been conserved across species, we found the behavior of CBG in squirrel monkey (Saimiri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00407a046
更新日期:1988-04-05 00:00:00
abstract::The distance between the calcium site (site I) and the zinc site (site II) in alpha-lactalbumin was estimated from Forster energy-transfer measurements between donor Eu(III) [or Tb(III)] at site I and acceptor Co(II) at site II to be 11.5 +/- 1.5 A. Intersite distances were also measured between the bis-ANS [4,4'-bis[...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00365a024
更新日期:1986-08-26 00:00:00
abstract::A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00218a034
更新日期:1991-01-29 00:00:00
abstract::A new fluorescence method to measure viscosity in cell cytosol [Fushimi, K., & Verkman, A. S. (1991) J. Cell Biol. 112, 719-725] has been applied to determine fluid-phase viscosity in sea urchin eggs. Freshly harvested eggs from Lytechinus pictus were loaded with the dyes 2,7-bis(2-carboxyethyl)-5-(and-6-)carboxyfluor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00115a600
更新日期:1991-12-24 00:00:00
abstract::We have shown previously that the hepatic and intestinal transcription of the human apolipoprotein A-II (apoA-II) gene in cell cultures is controlled by a complex set of regulatory elements A-N [Chambaz, et al. (1991) J. Biol. Chem. 266, 11676-11685; Cardot, et al. (1991) J. Biol. Chem. 266, 24460-24470]. In the prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00086a013
更新日期:1993-09-07 00:00:00
abstract::A set of peptides derived from the N-terminal domain of the ribosomal protein L9 (NTL9) have been characterized in an effort to define the minimum unit of this domain required to fold and to provide model peptides for the analysis of electrostatic interactions in the unfolded state. NTL9 is a 56-residue alpha-beta pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026410c
更新日期:2002-11-12 00:00:00
abstract::An alanine racemase encoded by a gene from the thermophilic Gram-positive bacterium Bacillus stearothermophilus is overproduced to 0.3% of the soluble protein when carried on plasmid pICR4 in Escherichia coli [Inagaki, K., Tanizawa, K., Badet, B., Walsh, C. T., Tanaka, H., & Soda, K. (1986) Biochemistry (third paper o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00359a029
更新日期:1986-06-03 00:00:00
abstract::Previous studies of Escherichia coli dihydrofolate reductase (ecDHFR) have demonstrated that residue G121, which is 19 A from the catalytic center, is involved in catalysis, and long distance dynamical motions were implied. Specifically, the ecDHFR mutant G121V has been extensively studied by various experimental and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0518242
更新日期:2006-02-07 00:00:00
abstract::The structures of synthetic peptides corresponding to regions of putative secondary structure in the cellular prion protein PrPC were studied as models for the conformational transition that features in the formation of the pathogenic isoform, PrPSc. Transgenetic studies argue that these PrP isoforms interact during t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00013a006
更新日期:1995-04-04 00:00:00
abstract::Tryptic digestion studies of the human erythrocyte glucose carrier have shown that a reactive and transport-sensitive exofacial sulfhydryl is located in the carboxy-terminal half of the molecule, corresponding to Cys347, Cys421, or Cys429. In the present studies, the erythrocyte glucose carrier labeled on the exofacia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00497a014
更新日期:1990-11-13 00:00:00
abstract::Although the short-chain dehydrogenase/reductase (SDR) superfamily contains a very large number of members defined in annotated databases and by biochemical and structural studies, very few SDR enzymes have been identified that have a homologous partner catalyzing the same reaction but with an opposite stereospecifici...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0118005
更新日期:2002-02-26 00:00:00
abstract::Ligand-dependent site-site (or subunit-subunit) interactions provide the basis for explaining cooperativity in chemical reactions. Even in the simplest possible nonaggregating system, interpretation of the interactions in terms of structural details requires an explicit assumption (or model) for the binding of the lig...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi00106a004
更新日期:1991-10-22 00:00:00
abstract::ISU-type proteins mediate cluster transfer to apo protein targets. Rate constants have been determined for cluster transfer from ISU to apo Fd for both Homo sapiens and Schizosaccharomyces pombe proteins, and cross reactions have also been examined. Substitution of a key aspartate residue of ISU is found to decrease t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0256781
更新日期:2002-07-16 00:00:00
abstract::Phospholipase A2 from Naja naja kaouthia venom was covalently coupled onto agarose beads using two different chemistries. The effect of micellar competitive inhibitors in the coupling media was evaluated. Enzyme bound to N-hydroxysuccinimide-activated agarose, which is reactive primarily toward epsilon-amino groups, h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00083a007
更新日期:1993-08-17 00:00:00
abstract::Thermodynamics of 39 oligonucleotides with internal G.T mismatches dissolved in 1 M NaCl were determined from UV absorbance versus temperature profiles. These data were combined with literature values of six sequences to derive parameters for 10 linearly independent trimer and tetramer sequences with G.T mismatches an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962590c
更新日期:1997-08-26 00:00:00
abstract::The hepatic metabolism of deuteriated D-methionine has been studied in the intact, anesthetized rat using 2H NMR spectroscopy. The rate of formation of the principal labeled metabolite, [methyl-2H3]sarcosine, from the D-[methyl-2H3]methionine precursor was found to be as rapid as the rate observed previously in NMR st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00420a042
更新日期:1988-10-04 00:00:00
abstract::The fluorescence self-quenching by energy transfer of FITC-C9, a fluoresceinated derivative of human complement protein C9 [Sims, P.J. (1984) Biochemistry (preceding paper in this issue)], has been used to monitor the kinetics of C9 polymerization induced by the membrane-associated complex of complement proteins C5b-8...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00309a021
更新日期:1984-07-03 00:00:00