当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Influence of chemistry in immobilization of cobra venom phospholipase A2: implications as to mechanism.

Influence of chemistry in immobilization of cobra venom phospholipase A2: implications as to mechanism.

Abstract：

:Phospholipase A2 from Naja naja kaouthia venom was covalently coupled onto agarose beads using two different chemistries. The effect of micellar competitive inhibitors in the coupling media was evaluated. Enzyme bound to N-hydroxysuccinimide-activated agarose, which is reactive primarily toward epsilon-amino groups, had 20% activity retention against micellar diheptanoylphosphatidylcholine (DiC7-PC). Enzyme bound through carboxylic groups, using a modification of the carbodiimide method, had 50% retention. Similar relative activities were observed, for both conjugates, in monomeric dihexanoyl-PC and in mixed micelles of Triton X-100 with dipalmitoyl-PC or dioleoylphosphatidylethanolamine. The soluble form of the enzyme showed premicellar activation against monomeric DiC7-PC, while the immobilized form showed interfacial recognition at concentrations around the critical micellar concentration. These results suggest that the enzyme activity lost upon immobilization is a result of the inherent chemical modification of the enzyme and that enzyme oligomerization and interfacial recognition are not cause-effect phenomena.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ferreira JP,Sasisekharan R,Louie O,Langer R

doi

10.1021/bi00083a007

subject

Has Abstract

pub_date

1993-08-17 00:00:00

pages

8098-102

issue

32

eissn

0006-2960

issn

1520-4995

journal_volume

32

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Conformational and aggregational properties of the gene 9 minor coat protein of bacteriophage M13 in membrane-mimicking systems.

    abstract：:The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized prote...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981149e

    authors： Houbiers MC,Spruijt RB,Wolfs CJ,Hemminga MA

    更新日期：1999-01-19 00:00:00

  • Isolation and structural determination of novel sulfated hexasaccharides from squid cartilage chondroitin sulfate E that exhibits neuroregulatory activities.

    abstract：:Squid cartilage chondroitin sulfate E (CS-E) exhibits various biological activities, including anticoagulant activities, lymphoid regulatory activities, and neuroregulatory activities [Ueoka, C., Kaneda, N., Okazaki, I., Nadanaka, S., Muramatsu, T., and Sugahara, K. (2000) J. Biol. Chem. 275, 37407-37413]. These activ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015577n

    authors： Kinoshita A,Yamada S,Haslam SM,Morris HR,Dell A,Sugahara K

    更新日期：2001-10-23 00:00:00

  • Interaction of the antitumor compound cryptophycin-52 with tubulin.

    abstract：:Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0010827

    authors： Panda D,Ananthnarayan V,Larson G,Shih C,Jordan MA,Wilson L

    更新日期：2000-11-21 00:00:00

  • Activation of overexpressed receptors for insulin and epidermal growth factor interferes in mitogenic signaling without affecting the activation of p21ras.

    abstract：:Activated receptors with a tyrosine kinase activity induce a variety of responses like changes in the differentiation and mitogenic status of cells. These responses are mediated in part by p21ras. Some of these activated receptors induce in certain cell types a pronounced, but transient, increase in Ras-GTP. We have s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00189a053

    authors： Osterop AP,Medema RH,Ouwens DM,Van der Zon GC,Möller W,Maassen JA

    更新日期：1994-06-14 00:00:00

  • Involvement of insulin-like growth factor type 1 receptor and protein kinase Cdelta in bis(maltolato)oxovanadium(IV)-induced phosphorylation of protein kinase B in HepG2 cells.

    abstract：:Vanadium(IV) oxo-bis(maltolato) (BMOV), an organovanadium compound, is a potent insulinomimetic agent and improves glucose homeostasis in various models of diabetes. We have shown previously that BMOV stimulates the phosphorylation of PKB which may contribute as one of the mechanisms for the insulinomimetic effect of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060403x

    authors： Mehdi MZ,Vardatsikos G,Pandey SK,Srivastava AK

    更新日期：2006-09-26 00:00:00

  • Solution structure of decorin-binding protein A from Borrelia burgdorferi.

    abstract：:Decorin-binding protein A (DBPA) is an important lipoprotein from the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. The absence of DBPA drastically reduces the pathogenic potential of the bacterium, and biochemical evidence indicates DBPA's interactions with the glycosaminoglycan (GAG) portion o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi3007093

    authors： Wang X

    更新日期：2012-10-23 00:00:00

  • The structural basis for pseudoreversion of the E165D lesion by the secondary S96P mutation in triosephosphate isomerase depends on the positions of active site water molecules.

    abstract：:The structural basis for the improvement in catalytic efficiency of the mutant E165D chicken triosephosphate isomerase by the secondary mutation, S96P, has been analyzed using a combination of X-ray crystallography and Fourier transform infrared spectroscopy. All X-ray structures were of the complex of phosphoglycoloh...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00041a041

    authors： Komives EA,Lougheed JC,Liu K,Sugio S,Zhang Z,Petsko GA,Ringe D

    更新日期：1995-10-17 00:00:00

  • Structure and inhibition of a quorum sensing target from Streptococcus pneumoniae.

    abstract：:Streptococcus pneumoniae 5'-methylthioadenosine/S-adenosylhomocysteine hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-methylthioribose or S-ribosylhomocysteine (SRH). MTAN is not found in mammals but is involved in bacterial quorum sensing. MTAN gene disruption affects ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061184i

    authors： Singh V,Shi W,Almo SC,Evans GB,Furneaux RH,Tyler PC,Painter GF,Lenz DH,Mee S,Zheng R,Schramm VL

    更新日期：2006-10-31 00:00:00

  • Dissociation of the α-subunit Calf-2 domain and the β-subunit I-EGF4 domain in integrin activation and signaling.

    abstract：:Integrin conformational changes mediate integrin activation and signaling triggered by intracellular molecules or extracellular ligands. Even though it is known that αβ transmembrane domain separation is required for integrin signaling, it is still not clear how this signal is transmitted from the transmembrane domain...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101462h

    authors： Wang W,Fu G,Luo BH

    更新日期：2010-11-30 00:00:00

  • Rational design of new binding specificity by simultaneous mutagenesis of calmodulin and a target peptide.

    abstract：:Calcium-saturated calmodulin (CaM) binds and influences the activity of a varied collection of target proteins in most cells. This promiscuity underlies the role of CaM as a shared participant in calcium-dependent signal transduction pathways but imposes a handicap on popular CaM-based calcium biosensors, which displa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060857u

    authors： Green DF,Dennis AT,Fam PS,Tidor B,Jasanoff A

    更新日期：2006-10-17 00:00:00

  • Cysteine biosynthetic enzymes are the pieces of a metabolic energy pump.

    abstract：:Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025953j

    authors： Wei J,Tang QX,Varlamova O,Roche C,Lee R,Leyh TS

    更新日期：2002-07-02 00:00:00

  • Structural basis for herbicidal inhibitor selectivity revealed by comparison of crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases.

    abstract：:A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049323o

    authors： Yang C,Pflugrath JW,Camper DL,Foster ML,Pernich DJ,Walsh TA

    更新日期：2004-08-17 00:00:00

  • Solvent effects on the kinetics and thermodynamics of stacking in poly(cytidylic acid).

    abstract：:The Raman laser temperature-jump technique has been used to measure the kinetics of the coil to helix reaction of poly(cytidylic acid) [poly(C)] in aqueous cosolvent mixtures. The rate of helix formation has a low activation energy and is proportional to reciprocal solvent viscosity. The observations suggest helix for...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00509a003

    authors： Freier SM,Hill KO,Dewey TG,Marky LA,Breslauer KJ,Turner DH

    更新日期：1981-03-17 00:00:00

  • The conserved seven-transmembrane sequence NP(X)2,3Y of the G-protein-coupled receptor superfamily regulates multiple properties of the beta 2-adrenergic receptor.

    abstract：:The beta 2-adrenergic receptor (beta 2AR) is a member of a large superfamily of seven transmembrane domain, G-protein-coupled receptors. Within the putative seventh transmembrane domain of the beta 2AR is a sequence of amino acids, NPLIY, which is conserved with minor variations in all members of the superfamily. Prev...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00047a003

    authors： Barak LS,Ménard L,Ferguson SS,Colapietro AM,Caron MG

    更新日期：1995-11-28 00:00:00

  • The BCL-2 5' untranslated region contains an RNA G-quadruplex-forming motif that modulates protein expression.

    abstract：:The BCL-2 gene encodes a 25 kDa membrane protein that plays critical roles in the control of apoptosis. The regulation of BCL-2 gene expression is highly complex and occurs both transcriptionally and posttranscriptionally. In particular, the 5' upstream region of BCL-2 contains a number of elements that control its ex...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100957h

    authors： Shahid R,Bugaut A,Balasubramanian S

    更新日期：2010-09-28 00:00:00

  • Binding and recognition of GATATC target sequences by the EcoRV restriction endonuclease: a study using fluorescent oligonucleotides and fluorescence polarization.

    abstract：:Oligonucleotides labeled with hexachlorofluorescein (hex) have enabled the interaction of the restriction endonuclease EcoRV with DNA to be evaluated using fluorescence anisotropy. The sensitivity of hex allowed measurements at oligonucleotide concentrations as low as 1 nM, enabling K(D) values in the low nanomolar ra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001956p

    authors： Reid SL,Parry D,Liu HH,Connolly BA

    更新日期：2001-02-27 00:00:00

  • Insights into the reaction mechanism of the prolyl-acyl carrier protein oxidase involved in anatoxin-a and homoanatoxin-a biosynthesis.

    abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200892a

    authors： Mann S,Lombard B,Loew D,Méjean A,Ploux O

    更新日期：2011-08-23 00:00:00

  • Involvement of serine 96 in the catalytic mechanism of ferredoxin-NADP+ reductase: structure--function relationship as studied by site-directed mutagenesis and X-ray crystallography.

    abstract：:The crystal structure of ferredoxin-NADP+ reductase (FNR) suggests that Ser96 is directly involved in hydride transfer between the isoalloxazine moiety of FAD and the nicotinamide ring of NADP(H). To probe its role, Ser96 has been mutated to valine (S96V) and glycine (S96G). These mutations primarily affected the inte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00026a019

    authors： Aliverti A,Bruns CM,Pandini VE,Karplus PA,Vanoni MA,Curti B,Zanetti G

    更新日期：1995-07-04 00:00:00

  • Role of ionic interactions in ligand binding and catalysis of R67 dihydrofolate reductase.

    abstract：:R67 dihydrofolate reductase (DHFR), which catalyzes the NADPH dependent reduction of dihydrofolate to tetrahydrofolate, belongs to a type II family of R-plasmid encoded DHFRs that confer resistance to the antibacterial drug trimethoprim. Crystal structure data reveals this enzyme is a homotetramer that possesses a sin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034643d

    authors： Hicks SN,Smiley RD,Hamilton JB,Howell EE

    更新日期：2003-09-16 00:00:00

  • Enzyme:substrate hydrogen bond shortening during the acylation phase of serine protease catalysis.

    abstract：:Atomic resolution (

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0517133

    authors： Fodor K,Harmat V,Neutze R,Szilágyi L,Gráf L,Katona G

    更新日期：2006-02-21 00:00:00

  • Enzymatic incorporation in DNA of 1,5-anhydrohexitol nucleotides.

    abstract：:The ability of several DNA polymerases to catalyze the template-directed synthesis of duplex oligonucleotides containing a base pair between a nucleotide with anhydrohexitol ring and its natural complement has been investigated. All DNA polymerases were able to accept the chemically synthesized anhydrohexitol triphosp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001297g

    authors： Vastmans K,Pochet S,Peys A,Kerremans L,Van Aerschot A,Hendrix C,Marlière P,Herdewijn P

    更新日期：2000-10-24 00:00:00

  • Xylose isomerase in substrate and inhibitor michaelis states: atomic resolution studies of a metal-mediated hydride shift.

    abstract：:Xylose isomerase (E.C. 5.3.1.5) catalyzes the interconversion of aldose and ketose sugars and has an absolute requirement for two divalent cations at its active site to drive the hydride transfer rates of sugar isomerization. Evidence suggests some degree of metal movement at the second metal site, although how this m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049812o

    authors： Fenn TD,Ringe D,Petsko GA

    更新日期：2004-06-01 00:00:00

  • Study of the conformational transition of A beta(1-42) using D-amino acid replacement analogues.

    abstract：:A critical event in Alzheimer's disease is the transition of Abeta peptides from their soluble forms into disease-associated beta-sheet-rich conformers. Structural analysis of a complete D-amino acid replacement set of Abeta(1-42) enabled us to localize in the full-length 42-mer peptide the region responsible for the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002005e

    authors： Janek K,Rothemund S,Gast K,Beyermann M,Zipper J,Fabian H,Bienert M,Krause E

    更新日期：2001-05-08 00:00:00

  • Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7.

    abstract：:The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701949p

    authors： Collino S,Kim IW,Evans JS

    更新日期：2008-03-25 00:00:00

  • Ca2+-signaling cycle of a membrane-docking C2 domain.

    abstract：:The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic signal-transducing proteins, including cytosolic phospholipase A2 (cPLA2) of the vertebrate inflammation pathway. Intracellular Ca2+ signals recruit the C2 domain of cPLA...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9717340

    authors： Nalefski EA,Slazas MM,Falke JJ

    更新日期：1997-10-07 00:00:00

  • Changes in conformation at the cytoplasmic ends of the fifth and sixth transmembrane helices of a yeast G protein-coupled receptor in response to ligand binding.

    abstract：:The third intracellular loop (IL3) of G protein-coupled receptors (GPCRs) is an important contact domain between GPCRs and their G proteins. Previously, the IL3 of Ste2p, a Saccharomyces cerevisiae GPCR, was suggested to undergo a conformational change upon activation as detected by differential protease susceptibilit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200254h

    authors： Umanah GK,Huang LY,Maccarone JM,Naider F,Becker JM

    更新日期：2011-08-16 00:00:00

  • Molecular structure of a fibrillar Alzheimer's A beta fragment.

    abstract：:Amyloid-beta (Abeta) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Abeta amyloid fibril using magnetically aligned preparations of a central Abeta domain which forms representative amyloid fibrils. Diffraction ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000637v

    authors： Serpell LC,Blake CC,Fraser PE

    更新日期：2000-10-31 00:00:00

  • Role of the electrophilic lipid peroxidation product 4-hydroxynonenal in the development and maintenance of obesity in mice.

    abstract：:The lipid peroxidation product 4-hydroxynonenal (4-HNE) is a signaling mediator with wide-ranging biological effects. In this paper, we report that disruption of mGsta4, a gene encoding the 4-HNE-conjugating enzyme mGSTA4-4, causes increased 4-HNE tissue levels and is accompanied by age-dependent development of obesit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702124u

    authors： Singh SP,Niemczyk M,Saini D,Awasthi YC,Zimniak L,Zimniak P

    更新日期：2008-03-25 00:00:00

  • The interface between the EGF2 domain and the protease domain in blood coagulation factor IX contributes to factor VIII binding and factor X activation.

    abstract：:The light chain of activated factor IX (FIXa) is involved in a number of functional properties, including FIXa enzymatic activity. This suggests the existence of a functional link between the FIXa light chain and the catalytic domain. The FIXa structure includes a few putative interactions between EGF2 and the proteas...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060451h

    authors： Fribourg C,Meijer AB,Mertens K

    更新日期：2006-09-05 00:00:00

  • Evidence that catalysis by yeast inorganic pyrophosphatase proceeds by direct phosphoryl transfer to water and not via a phosphoryl enzyme intermediate.

    abstract：:In this work, we show that adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) is a substrate for yeast inorganic pyrophosphatase (PPase) (EC 3.6.1.1) and further, using chirally labeled [gamma-17O,18O]ATP gamma S, that enzyme-catalyzed hydrolysis to produce chiral inorganic thio[17O,18O]phosphate proceeds with inversio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00300a002

    authors： Gonzalez MA,Webb MR,Welsh KM,Cooperman BS

    更新日期：1984-02-28 00:00:00