Abstract：
:We report the genetically encoded chemical decaging strategy for protein activation in living bacterial cells. In contrast to the metabolically labile photocaging groups inside Escherichia coli, our chemical decaging strategy that relies on the inverse electron-demand Diels-Alder (iDA) reaction is compatible with the intracellular environment of bacteria, which can be a general tool for gain-of-function study of a given protein in prokaryotic systems. By applying this strategy for in situ activation of the indole-producing enzyme TnaA, we built an orthogonal and chemically inducible indole production pathway inside E. coli cells, which revealed the role of indole in bacterial antibiotic tolerance.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Liu L,Liu Y,Zhang G,Ge Y,Fan X,Lin F,Wang J,Zheng H,Xie X,Zeng X,Chen PRdoi
10.1021/acs.biochem.7b01017subject
Has Abstractpub_date
2018-01-30 00:00:00pages
446-450issue
4eissn
0006-2960issn
1520-4995journal_volume
57pub_type
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