Duplication of a Single Strand in a β-Sheet Can Produce a New Switching Function in a Photosensory Protein.

abstract：:S100A1, a member of the S100 protein family, is an EF-hand containing Ca(2+)-binding protein (93 residues per subunit) with noncovalent interactions at its dimer interface. Each subunit of S100A1 has four alpha-helices and a small antiparallel beta-sheet consistent with two helix-loop-helix calcium-binding domains [Ba...

journal_title：Biochemistry

authors： Rustandi RR,Baldisseri DM,Inman KG,Nizner P,Hamilton SM,Landar A,Landar A,Zimmer DB,Weber DJ

更新日期：2002-01-22 00:00:00

abstract：:Glycosylases specifically recognize and flip their target base out of the DNA helix into the enzyme's active site. Our simulations show that a partially flipped state, already present in free DNA carrying a T:G mispair, becomes the more probable state compared to the closed state after binding of thymine DNA glycosyla...

journal_title：Biochemistry

authors： Kanaan N,Imhof P

更新日期：2018-10-02 00:00:00

abstract：:Dihydroorotate dehydrogenases (DHODs) oxidize dihydroorotate (DHO) to orotate using the FMN prosthetic group to abstract a hydride equivalent from C6 and a protein residue (Ser for Class 2 DHODs) to deprotonate C5. The fundamental question of whether the scission of the two DHO C-H bonds is concerted or stepwise was a...

journal_title：Biochemistry

authors： Fagan RL,Nelson MN,Pagano PM,Palfey BA

更新日期：2006-12-19 00:00:00

abstract：:Pseudokinases play important roles in signal transduction and cellular processes similar to those of catalytically competent kinases. However, pseudokinase pharmacological tractability and conformational space accessibility are poorly understood. Pseudokinases have only recently been suggested to adopt "inactive" conf...

journal_title：Biochemistry

authors： Smith RHB,Khan ZM,Ung PM,Scopton AP,Silber L,Mack SM,Real AM,Schlessinger A,Dar AC

更新日期：2021-01-13 00:00:00

abstract：:Gadolinium (III) binds competitively with calcium(II) to porcine pancreatic phospholipase A2 (EC 3.1.1.4) and its zymogen. The enzyme-Gd3+ complex exhibits 4% of the hydrolytic activity of the corresponding Ca2+ complex toward a dispersion of dioctanoyllecithin. Dissociation constants for the Gd3+ complex of enzyme an...

journal_title：Biochemistry

authors： Hershberg RD,Reed GH,Slotboom AJ,deHaas GH

更新日期：1976-06-01 00:00:00

abstract：:Steroid-receptor complexes formed at low temperature and ionic strength are unable to bind to target cell nuclei. After a temporary exposure to high ionic strength and/or temperature they become activated (i.e., able to bind to nuclei). However, there exists an equilibrium between activated and nonactivated complexes;...

journal_title：Biochemistry

authors： Atger M,Milgrom E

更新日期：1976-09-21 00:00:00

abstract：:Activation of a growth factor receptor tyrosine kinase (RTK) is accompanied by a rapid autophosphorylation of the receptor on tyrosine residues. Receptor activation has been shown to promote the association of signal-transducing proteins containing SH2 domains (second domain of src homology). These receptor-associated...

journal_title：Biochemistry

authors： Sierke SL,Koland JG

更新日期：1993-09-28 00:00:00

abstract：:In synthetic biology, metabolic engineering, and gene therapy, there is a strong demand for orthogonal or externally controlled regulation of gene expression. Here, RNA-based regulatory devices represent a promising emerging alternative to proteins, allowing a fast and direct control of gene expression, as no synthesi...

journal_title：Biochemistry

authors： Etzel M,Mörl M

更新日期：2017-03-07 00:00:00

abstract：:Volumetric characteristics of protein recognition events determine the direction of pressure-induced shifts in the recognition reaction, while also providing insights into the structural, dynamic, and hydration changes. We report changes in volume, ΔV, and adiabatic compressibility, ΔK(S), accompanying the binding of ...

journal_title：Biochemistry

authors： Son I,Shek YL,Dubins DN,Chalikian TV

更新日期：2012-07-24 00:00:00

abstract：:The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with...

journal_title：Biochemistry

authors： Vertessy BG,Harmat V,Böcskei Z,Náray-Szabó G,Orosz F,Ovádi J

更新日期：1998-11-03 00:00:00

abstract：:The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (197...

journal_title：Biochemistry

authors： Wright AK,Thompson MR,Miller RL

更新日期：1975-07-15 00:00:00

abstract：:The water-oxidizing complex of photosystem II cycles through five oxidation states, denoted S(i)() (i = 0-4), during water oxidation to molecular oxygen, which appears at the (transient) S(4) state. The recent detection of bimodal EPR signals from the S(3) state [Matsukawa, T., Mino, H., Yoneda, D., Kawamori, A. (1999...

journal_title：Biochemistry

authors： Ioannidis N,Petrouleas V

更新日期：2002-07-30 00:00:00

abstract：:A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...

journal_title：Biochemistry

authors： Selden LA,Kinosian HJ,Estes JE,Gershman LC

更新日期：2000-01-11 00:00:00

abstract：:Interaction of a mitochondria-specific anionic phospholipid, cardiolipin (CL), with an intermembrane protein, cytochrome c (cyt c), yields a peroxidase complex. During apoptosis, the complex induces accumulation of CL oxidation products that are essential for detachment of cyt c from the mitochondrial membrane, induct...

journal_title：Biochemistry

authors： Borisenko GG,Kapralov AA,Tyurin VA,Maeda A,Stoyanovsky DA,Kagan VE

更新日期：2008-12-23 00:00:00

abstract：:The presence of a N-(2-deoxy-D-erythro pentofuranosyl)urea (henceforth referred to as deoxyribosylurea) residue, ring fragmentation product of a thymine, in a frameshift situation in the sequence 5'd(AGGACCACG).d(CGTGGurTCCT) has been studied by 1H and 31P nuclear magnetic resonance and molecular dynamics. At equilibr...

journal_title：Biochemistry

authors： Gervais V,Cognet JA,Guy A,Cadet J,Téoule R,Fazakerley GV

更新日期：1998-01-27 00:00:00

abstract：:In recent years, a number of bioorthogonal reactions have been developed, exemplified by click chemistry, that enable the efficient formation of a specific product, even within a highly complex chemical environment. While the exquisite selectivity and reliability of these transformations have led to their broad applic...

journal_title：Biochemistry

authors： Best MD

更新日期：2009-07-21 00:00:00

abstract：:In the Arc repressor dimer, the side chains of Ile37 and Val41 in alpha-helix B pack against each other and against the symmetry-related side chains of Ile37' and Val41' in alpha-helix B' to form part of the hydrophobic core and the dimer interface. Following combinatorial mutagenesis of these positions, only the wild...

journal_title：Biochemistry

authors： Milla ME,Sauer RT

更新日期：1995-03-14 00:00:00

abstract：:Steroidogenic acute regulatory protein (StAR) rapidly stimulates the movement of cholesterol into adrenal and gonadal mitochondria to mediate the acute steroidogenic response; StAR mutations cause potentially lethal congenital lipoid adrenal hyperplasia (lipoid CAH). Bacterially expressed wild-type StAR and four amino...

journal_title：Biochemistry

authors： Bose HS,Baldwin MA,Miller WL

更新日期：1998-07-07 00:00:00

abstract：:CO dehydrogenase from Clostridium thermoaceticum is a nickel-containing enzyme that catalyzes both the reversible conversion of CO2 to CO (for incorporation into the carbonyl group of acetate) and the synthesis of acetyl-CoA from methyl corrinoid, CO, and CoASH. The latter activity is conveniently assayed by monitorin...

journal_title：Biochemistry

authors： Raybuck SA,Bastian NR,Orme-Johnson WH,Walsh CT

更新日期：1988-10-04 00:00:00

abstract：:The roles of four of the active center groups with potential acid-base properties in the region of pH optimum of pyruvate decarboxylase from Saccharomyces cerevisiae have been studied with the substitutions Asp28Ala, His114Phe, His115Phe, and Glu477Gln, introduced by site-directed mutagenesis methods. The steady-state...

journal_title：Biochemistry

authors： Liu M,Sergienko EA,Guo F,Wang J,Tittmann K,Hübner G,Furey W,Jordan F

更新日期：2001-06-26 00:00:00

abstract：:Chemical deglycosylation of gonadotropins with hydrogen fluoride (HF) has facilitated the investigation of the structure-function relationship of the individual peptide and oligosaccharide moieties in the mechanism of hormone action. These studies have dealt almost exclusively with lutropin or human choriogonadotropin...

journal_title：Biochemistry

authors： Calvo FO,Keutmann HT,Bergert ER,Ryan RJ

更新日期：1986-07-01 00:00:00

abstract：:Heparan sulfate (HS) proteoglycans (PGs) interact with a number of extracellular signaling proteins, thereby playing an essential role in the regulation of many physiological processes. These interactions are important for both normal signal transduction and regulation of the tissue distribution of signaling molecules...

journal_title：Biochemistry

authors： Zhang F,McLellan JS,Ayala AM,Leahy DJ,Linhardt RJ

更新日期：2007-04-03 00:00:00

abstract：:N-RAP is a recently discovered muscle-specific protein that is concentrated at the myotendon junctions in skeletal muscle and at the intercalated disks in cardiac muscle. The C-terminal half of N-RAP contains a region with sequence homology to nebulin, while a LIM domain is found at its N-terminus. N-RAP is hypothesiz...

journal_title：Biochemistry

authors： Luo G,Herrera AH,Horowits R

更新日期：1999-05-11 00:00:00

abstract：:More than 100 distinct mutations in the gene CuZnSOD encoding human copper-zinc superoxide dismutase (CuZnSOD) have been associated with familial amyotrophic lateral sclerosis (fALS), a fatal neuronal disease. Many studies of different mutant proteins have found effects on protein stability, catalytic activity, and me...

journal_title：Biochemistry

authors： Hennig J,Andrésen C,Museth AK,Lundström P,Tibell LA,Jonsson BH

更新日期：2015-01-20 00:00:00

abstract：:Nitrogenase reduces N2 to NH3, but the mechanistic details are unclear. Diazene (N2H2), a proposed 2e-/2H+ intermediate on the reduction pathway, is labile under typical enzyme assay conditions, and no firm evidence is available on whether or not it can be reduced by or inhibit nitrogenase. In this paper, we compare t...

journal_title：Biochemistry

authors： McKenna CE,Simeonov AM,Eran H,Bravo-Leerabhandh M

更新日期：1996-04-09 00:00:00

abstract：:The binding of formate ion, a substrate for the peroxidatic reaction of catalase, has been investigated by magnetic resonance techniques. Comparative studies of formate binding to ferric myoglobin have also been performed. The nuclear magnetic relaxation (NMR) rate of formate and water protons is enhanced by the prese...

journal_title：Biochemistry

authors： Hershberg RD,Chance B

更新日期：1975-08-26 00:00:00

abstract：:The site-specific chemical modification of NADH-quinone oxidoreductase (complex I) by various functional probes such as fluorophores and microbeads, without affecting the enzyme activity, may allow single-molecule analyses of putative dynamic conformational changes in the enzyme. In an attempt to address this challeng...

journal_title：Biochemistry

authors： Masuya T,Murai M,Ifuku K,Morisaka H,Miyoshi H

更新日期：2014-04-15 00:00:00

abstract：:The downfield (9-15 ppm) proton spectrum of Escherichia coli 5S RNA has been examined at 500 MHz by using nuclear Overhauser methods. The data confirm the existence of the terminal and procaryotic loop helices within the molecule [Fox, G. E., & Woese, C. R. (1975) Nature (London) 256, 505-506]. Very little stable, dou...

journal_title：Biochemistry

authors： Kime MJ,Moore PB

更新日期：1983-05-24 00:00:00

abstract：:Sialyltransferase activities, SAT-3 (CMP-NeuAc:nLcOse4Cer alpha 2-3sialyltransferase) and SAT-4 (CMP-NeuAc:GgOse4Cer alpha 2-3sialyltransferase), in Colo 205 cells catalyze the transfer of sialic acid to the terminal galactose of GlcNc-- and GalNAc-containing glycolipid substrates, respectively. Competition kinetic st...

journal_title：Biochemistry

authors： Basu SS,Basu M,Li Z,Basu S

更新日期：1996-04-23 00:00:00

abstract：:A study of the dynamics and mechanism of the various thermotropic phase transitions undergone by the hydrated monoacylglycerides monoolein and monoelaidin, in the temperature range of 20-120 degrees C and from 0 to 5 M NaCl, has been undertaken. Measurements were made by using time-resolved X-ray diffraction at the Co...

journal_title：Biochemistry

authors： Caffrey M

更新日期：1987-10-06 00:00:00