A study of protein-sodium dodecyl sulfate complexes by transient electric birefringence.

Abstract:

:The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (1970), J. Biol. Chem. 245, 5161) and K. Shirahama, K. Tsujii, and T. Takagi (1974, J. Biochem. 75, 309). Differences between these latter two models are resolved by the model presented here. In addition, it has been demonstrated that protein molecular weights may be obtained from the slow relaxation time for transient electric birefringence of protein-dodecyl sulfate complexes.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Wright AK,Thompson MR,Miller RL

doi

10.1021/bi00685a030

subject

Has Abstract

pub_date

1975-07-15 00:00:00

pages

3224-8

issue

14

eissn

0006-2960

issn

1520-4995

journal_volume

14

pub_type

杂志文章