Abstract:
:Chemical deglycosylation of gonadotropins with hydrogen fluoride (HF) has facilitated the investigation of the structure-function relationship of the individual peptide and oligosaccharide moieties in the mechanism of hormone action. These studies have dealt almost exclusively with lutropin or human choriogonadotropin. We report here the chemical characterization and biological properties of deglycosylated human follitropin (degly hFSH). Results indicate that deglycosylation of hFSH by HF removes 89% of the total carbohydrate without disruption of the peptide chain or significant loss of amino acid residues. However, a change in the conformation of the molecule was observed by measurement of the far-ultraviolet circular dichroic spectrum. The degly hFSH showed a 44% reduction in binding when tested in a FSH radioimmunoassay utilizing a polyclonal antibody. Binding of the degly hFSH to FSH-responsive tissues showed that the altered hormone bound with equal or better avidity than the intact hormone while the association constants were approximately the same for both preparations. The degly hFSH alone did not stimulate the FSH-stimulatable adenylyl cyclase (AC) activity of cellular homogenates of small follicle porcine granulosa cells. Furthermore, degly hFSH was a potent antagonist of hFSH-stimulatable AC activity when coincubated with intact hFSH. In intact granulosa cells, both the hFSH and the degly hFSH stimulated cAMP production and release by these cells. However, the degly hFSH was one-tenth as effective as the intact hormone.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Calvo FO,Keutmann HT,Bergert ER,Ryan RJdoi
10.1021/bi00361a030subject
Has Abstractpub_date
1986-07-01 00:00:00pages
3938-43issue
13eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Neuroligins 1-4 are postsynaptic transmembrane proteins capable of initiating presynaptic maturation via interactions with beta-neurexin. Both neuroligins and beta-neurexins have alternatively spliced inserts in their extracellular domains. Using analytical ultracentrifugation, we determined that the extracellular dom...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0614131
更新日期:2006-10-24 00:00:00
abstract::The biosynthesis of the antitumor antibiotic sibiromycin by Streptosporangium sibiricum requires the construction of four units: the amino sugar from glucose; the anthranilate ring from DL-tryptophan probably via kynurenine; the aromatic methyl group from methionine; the propylidene proline from L-tyrosine with the lo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00586a029
更新日期:1979-09-18 00:00:00
abstract::Electrogenic events associated with the activity of the melibiose permease (MelB), a transporter from Escherichia coli, were investigated. Proteoliposomes containing purified MelB were adsorbed to a solid supported lipid membrane, activated by a substrate concentration jump, and transient currents were measured. When ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0489053
更新日期:2004-10-05 00:00:00
abstract::Fluorescent derivatives of short oligonucleotides of defined sequence were prepared by the incorporation of 5-(propylamino)uridine via current phosphoramidite chemistry, followed by derivatization of the propylamine function with mansyl chloride. These oligomers, annealed to complementary oligomers, yielded short dupl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00437a014
更新日期:1989-05-30 00:00:00
abstract::The phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc) is a 28.5 kDa enzyme with three zinc ions in its active site. Although much is known about the roles that various PLCBc active site amino acids play in binding and catalysis, there is little information about the rate-determining step of t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9821216
更新日期:1999-04-06 00:00:00
abstract::The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025722c
更新日期:2002-06-18 00:00:00
abstract::Maltosyl isothiocyanate (MITC) has been synthesized from maltose with an overall yield of 88%. It has been found to be a potent irreversible inhibitor of zero trans influx of glucose with human erythrocytes. Kinetic analysis of glucose transport after treatment of erythrocytes with MITC revealed that VT was diminished...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00547a025
更新日期:1980-03-18 00:00:00
abstract::Ionizing radiation, free radicals, and reactive oxygen species produce hundreds of different DNA lesions. Clustered lesions are typical for ionizing radiation. They compromise the efficiency of the base excision repair (BER) pathway, and as a consequence, they are much more toxic and mutagenic than isolated lesions. D...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00396
更新日期:2016-07-19 00:00:00
abstract::Molecular interactions between the tumor suppressor p53 and the transcriptional coactivators CBP/p300 are critical for the regulation of p53 transactivation and stability. The transactivation domain (TAD) of p53 binds directly to several CBP/p300 domains (TAZ1, TAZ2, NCBD, and KIX). Here we map the interaction between...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802055v
更新日期:2009-03-17 00:00:00
abstract::The mitochondrial tyrosyl tRNA synthetase from Neurospora crassa (CYT-18 protein) is a bifunctional group I intron splicing cofactor. CYT-18 is capable of splicing multiple group I introns from a wide variety of sources by stabilizing the catalytically active intron structures. CYT-18 and mt TyrRSs from related fungal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401614h
更新日期:2014-03-04 00:00:00
abstract::Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a025
更新日期:1977-04-05 00:00:00
abstract::Earlier thermodynamic studies of the intermolecular interactions between mature oxytocin and neurophysin, and of the effects of these interactions on neurophysin folding, raised questions about the intramolecular interactions of oxytocin with neurophysin within their common precursor. To address this issue, the disulf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9912950
更新日期:1999-10-12 00:00:00
abstract::Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00198a032
更新日期:1994-08-16 00:00:00
abstract::The quaternary structures of Escherichia coli DNA-dependent RNA polymerase holenzyme (alpha 2 beta beta' sigma) and core enzyme (alpha 2 beta beta') have been investigated by chemical cross-linking with a cleavable bifunctional reagent, methyl 4-mercaptobutyrimidate, and noncleavable reagents, dimethyl suberimidate an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00634a008
更新日期:1977-07-26 00:00:00
abstract::A site-specific analog of ATP, 6,6'-dithiobis (inosinyl imidodiphosphate (S2P-PNP), inactivates the ATPase activities of myosin's proteolytic fragments, heavy meromyosin (HMM) and subfragment one (SF1), by formation of mixed disulfides between the 6 position of the purine ring and certain key cysteines. The stoichiome...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00680a015
更新日期:1975-05-06 00:00:00
abstract::Heme catabolism is an important biochemical process that many bacterial pathogens utilize to acquire iron. However, tetrapyrrole catabolites can be reactive and often require further processing for transport out of the cell or conversion to another useful cofactor. In previous work, we presented in vitro evidence of a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01099
更新日期:2017-02-14 00:00:00
abstract::Three distinct fibrinolytic enzymes have been purified from the venom of Crotalus basiliscus basiliscus (the Mexican west coast rattlesnake). The high-performance liquid chromatography-based purification comprised the following steps: (a) hydrophobic interaction chromatography; (b) hydroxylapatite chromatography; (c) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00134a003
更新日期:1992-05-19 00:00:00
abstract::The aspartate receptor is one of the ligand-specific, homodimeric chemoreceptors that detects extracellular attractants and triggers the chemotaxis pathway of Escherichia coli and Salmonella typhimurium. This receptor regulates the activity of the histidine kinase CheA, which forms a kinetically stable complex with th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0507884
更新日期:2005-09-27 00:00:00
abstract::The antimicrobial peptide MSI-78 serves as a model system for studying interactions of bioactive peptides with membranes. Using a series of MSI-78 peptides that incorporate l-4,4,4-trifluoroethylglycine, a small and sensitive (19)F nuclear magnetic resonance probe, we investigated how the local structure and dynamics ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200639c
更新日期:2011-07-12 00:00:00
abstract::The following peptide was synthesized by classical methods in solution: Ac-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg-Gly-Pro-Arg-Val-NHCH3 (F-8). The Michaelis-Menten parameters for the hydrolysis of the Arg-Gly bond in F-8 by thrombin were determined to be Kcat = 31 X 10(-11) M [(NIH unit/L) s]-1 and KM = 310 X 10(-6) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00287a002
更新日期:1983-08-30 00:00:00
abstract::The transfer of cytochrome P-450 from bovine adrenocortical submitochondrial particles (smp) to unilamellar liposome membranes was investigated using a table top ultracentrifuge. Submitochondrial particles were incubated with liposome membranes at 25 degrees C and precipitated by ultracentrifugation at 200000g for a f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00014a041
更新日期:1995-04-11 00:00:00
abstract::The cystic fibrosis transmembrane conductance regulator (CFTR) functions in vivo as a cAMP-activated chloride channel. A member of the ATP-binding cassette superfamily of membrane transporters, CFTR contains two transmembrane domains (TMDs), two nucleotide-binding domains (NBDs), and a regulatory (R) domain. It is pre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035382a
更新日期:2004-02-03 00:00:00
abstract::The three-dimensional structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica, has been determined in solution at pH 6.8 and 25 degrees C by (1)H and (15)N NMR spectroscopy. The main body (Glu13-Asp97) of oryzacystatin-I is well-defined and consists of an alpha-helix and a f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0006971
更新日期:2000-12-05 00:00:00
abstract::The calcitonin gene-related peptide (CGRP) receptor is a heterodimer of two membrane proteins: calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). CLR is a class B G-protein-coupled receptor (GPCR), possessing a characteristic large amino-terminal extracellular domain (ECD) impor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901848m
更新日期:2010-03-09 00:00:00
abstract::The structural underpinnings of enzyme substrate specificity are investigated in a pair of copper amine oxidases (CAOs) from Hansenula polymorpha (HPAO-1 and HPAO-2). The X-ray crystal structure (to 2.0 A resolution) and steady state kinetic data of the second copper amine oxidase (HPAO-2) are presented for comparison...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901933d
更新日期:2010-03-23 00:00:00
abstract::The malarial parasite Plasmodium falciparum can only synthesize pyrimidine nucleotides via the de novo pathway which is therefore a suitable target for development of antimalarial drugs. New assay procedures have been developed using high-pressure liquid chromatography (HPLC) which enable concurrent measurement of pyr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00183a033
更新日期:1994-05-03 00:00:00
abstract::Thrombin-activatable fibrinolysis inhibitor (TAFI) circulates as an inactive proenzyme of a carboxypeptidase B-like enzyme (TAFIa). It functions by removing C-terminal lysine residues from partially degraded fibrin that are important in tissue-type plasminogen activator mediated plasmin formation. TAFI was classified ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0115683
更新日期:2002-01-29 00:00:00
abstract::The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0500832
更新日期:2005-06-21 00:00:00
abstract::Cytochrome b559 (cyt b559) is an intrinsic and essential component of the photosystem II (PSII) protein complex, but its function, stoichiometry, and electron-transfer kinetics in the physiological system are not well-defined. In this study, we have used flash-detection optical spectroscopy to measure the kinetics and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00161a025
更新日期:1992-11-24 00:00:00
abstract::Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701954p
更新日期:2008-01-08 00:00:00