Exploring the role and the binding affinity of a second zinc equivalent in B. cereus metallo-beta-lactamase.

abstract：:The reactions of nitric oxide (NO) with the turnover intermediates of cytochrome c oxidase were investigated by combining amperometric and spectroscopic techniques. We show that the complex of nitrite with the oxidized enzyme (O) is obtained by reaction of both the "peroxy" (P) and "ferryl" (F) intermediates with stoi...

journal_title：Biochemistry

authors： Giuffrè A,Barone MC,Mastronicola D,D'Itri E,Sarti P,Brunori M

更新日期：2000-12-19 00:00:00

abstract：:The human tear fluid film consists of a superficial lipid layer, an aqueous middle layer, and a hydrated mucin layer located next to the corneal epithelium. The superficial lipid layer protects the eye from drying and is composed of polar and neutral lipids provided by the meibomian glands. Excess accumulation of lipi...

journal_title：Biochemistry

authors： Jauhiainen M,Setälä NL,Ehnholm C,Metso J,Tervo TM,Eriksson O,Holopainen JM

更新日期：2005-06-07 00:00:00

abstract：:We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. (1992) FEBS Lett. 304, 256-260]. Like [gamma-32P]ATP, [gamma-32P]GTP ...

journal_title：Biochemistry

authors： Lelong IH,Cardarelli CO,Gottesman MM,Pastan I

更新日期：1994-08-02 00:00:00

abstract：:The heme and cysteine microenvironments of bluefin tuna apomyoglobin have been investigated by examining the fluorescence properties of two extrinsic chromophores, i.e., ANS and 1,5-AEDANS. 1,5-AEDANS was covalently bound to the single cysteine residue found in the primary structure of tuna apomyoglobin. Recombination...

journal_title：Biochemistry

authors： Colonna G,Balestrieri C,Bismuto E,Servillo L,Irace G

更新日期：1982-01-19 00:00:00

abstract：:Reduced intracellular drug accumulation due to the activity of the drug efflux pump ABC (B1) is a major mechanism in the resistance of cancer cells to chemotherapy. ABC (B1) is a poly specific transporter, and the molecular mechanism of its complex translocation process remains to be elucidated. To understand the proc...

journal_title：Biochemistry

authors： Storm J,Modok S,O'Mara ML,Tieleman DP,Kerr ID,Callaghan R

更新日期：2008-03-25 00:00:00

abstract：:LTbetaR is a member of the TNF receptor family of proteins. It binds to two different cell surface ligands, LIGHT, a homotypic trimer, and LTalpha1beta2, a heterotypic trimer. We have measured the affinities of the dimeric IgG fusion protein, LTbetaRIgG, and monomeric LTbetaR protein binding to both LIGHT and LTalpha1...

journal_title：Biochemistry

authors： Eldredge J,Berkowitz S,Corin AF,Day ES,Hayes D,Meier W,Strauch K,Zafari M,Tadi M,Farrington GK

更新日期：2006-08-22 00:00:00

abstract：:The ZFY protein is a member of one of the most interesting classes of polydactyl zinc finger proteins. It has a domain of 13 tandem zinc fingers that is organized with an internal repeat of odd-even finger pairs. It has been proposed that each finger pair interacts with six base pairs within a turn of the double helix...

journal_title：Biochemistry

authors： Grants J,Flanagan E,Yee A,Romaniuk PJ

更新日期：2010-02-02 00:00:00

abstract：:We found that recombinant human adult hemoglobin (rHb A) expressed in Escherichia coli showed heterogeneity of components with the intensity of a positive CD band at 260 nm and that it could be resolved into three components (SP-1, SP-2, and SP-3) by SP-Sepharose column chromatography. 1H NMR revealed that SP-1 is ide...

journal_title：Biochemistry

authors： Nagai M,Nagai Y,Aki Y,Imai K,Wada Y,Nagatomo S,Yamamoto Y

更新日期：2008-01-15 00:00:00

abstract：:A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 h...

journal_title：Biochemistry

authors： Musila JM,L Forbes D,Ellis HR

更新日期：2018-07-31 00:00:00

abstract：:Recent studies of DNA hairpin loops have shown considerable dependence of the stability on the sequence of the loop [Senior, M., Jones, R. A., & Breslauer, K. J. (1988a) Proc. Natl. Acad. Sci. U.S.A. 85, 6242-6246; Xodo, L. E., Manzini, G., Quadrifoglio, F., van der Marel, G., & van Boom, J. H. (1989) Biochimie 71, 79...

journal_title：Biochemistry

authors： Wang S,Booher MA,Kool ET

更新日期：1994-04-19 00:00:00

abstract：:Nhp6A is an abundant non-histone chromatin-associated protein in Saccharomyces cerevisiae that contains a minor groove DNA binding motif called the HMG box. In this report, we show that Nhp6Ap binds to cisplatin intrastrand cross-links on duplex DNA with a 40-fold greater affinity than to unmodified DNA with the same ...

journal_title：Biochemistry

authors： Wong B,Masse JE,Yen YM,Giannikopoulos P,Feigon J,Johnson RC

更新日期：2002-04-30 00:00:00

abstract：:The light-induced global conformational change of photoactive yellow protein was directly observed by small-angle X-ray scattering (SAXS). The N-terminal 6, 15, or 23 amino acid residues were enzymatically truncated (T6, T15, or T23, respectively), and their near-UV intermediates were accumulated under continuous illu...

journal_title：Biochemistry

authors： Imamoto Y,Kamikubo H,Harigai M,Shimizu N,Kataoka M

更新日期：2002-11-19 00:00:00

abstract：:Retinoid X receptors (RXRs) are nuclear receptors that can activate transcription as homodimers or as obligate heterodimeric partners of other nuclear receptors. While the crystal structures of the RXR ligand-binding domains (LBD) have been previously determined, the dynamics of activation is less well characterized a...

journal_title：Biochemistry

authors： Lu J,Cistola DP,Li E

更新日期：2006-02-14 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors that exhibit photochromism between two states: a thermally stable dark-adapted state and a metastable light-adapted state with bound linear tetrapyrrole (bilin) chromophores possessing 15Z and 15E configurations, respectively. The photodynamics of canonica...

journal_title：Biochemistry

authors： Kirpich JS,Chang CW,Franse J,Yu Q,Escobar FV,Jenkins AJ,Martin SS,Narikawa R,Ames JB,Lagarias JC,Larsen DS

更新日期：2021-01-13 00:00:00

abstract：:The role of acidic amino acid residues in cation recognition and selectivity by the Na+/dicarboxylate cotransporter, NaDC-1, was investigated by site-directed mutagenesis and expression in Xenopus oocytes. Four of the residues tested, Asp-52, Glu-74, Glu-101, and Glu-332, were found to be unimportant for transport act...

journal_title：Biochemistry

authors： Griffith DA,Pajor AM

更新日期：1999-06-08 00:00:00

abstract：:Nonenzymatic deamidation of asparagine and glutamine in peptides and proteins is a frequent modification both in vivo and in vitro. The biological effect is not completely understood, but it is often associated with protein degradation and loss of biological function. Here we describe the deamidation of CsgA, the majo...

journal_title：Biochemistry

authors： Wang H,Shu Q,Frieden C,Gross ML

更新日期：2017-06-13 00:00:00

abstract：:Anaerobic induction of light harvesting and reaction center gene expression involves two transacting factors termed RegA and RegB. Sequence and mutational analysis has indicated that RegA and RegB constitute cognate components of a prokaryotic sensory transduction cascade with RegB comprising a membrane-spanning senso...

journal_title：Biochemistry

authors： Inoue K,Kouadio JL,Mosley CS,Bauer CE

更新日期：1995-01-17 00:00:00

abstract：:To determine the active site cysteine residue in aldehyde dehydrogenase, we mutated amino acid residues 49, 162, and 302 of recombinantly expressed rat liver mitochondrial (class 2) aldehyde dehydrogenase. The C49A and C162A mutants were fully active tetrameric enzymes, although the C162A mutant was found to be highly...

journal_title：Biochemistry

authors： Farrés J,Wang TT,Cunningham SJ,Weiner H

更新日期：1995-02-28 00:00:00

abstract：:Engineering proteins to withstand a broad range of conditions continues to be a coveted objective, holding the potential to advance biomedicine, industry, and our understanding of disease. One way of achieving this goal lies in elucidating the underlying interactions that define protein stability. It has been shown th...

journal_title：Biochemistry

authors： Strickler SS,Gribenko AV,Gribenko AV,Keiffer TR,Tomlinson J,Reihle T,Loladze VV,Makhatadze GI

更新日期：2006-03-07 00:00:00

abstract：:We have examined the reduction of cyanide by using the purified component proteins of nitrogenase (Av1 and Av2). The previously reported self-inhibition phenomenon was found to be an artifact. One of the two species present in cyanide solutions, CN-, was shown to be a potent reversible inhibitor (Ki = 27 microM) of to...

journal_title：Biochemistry

authors： Li J,Burgess BK,Corbin JL

更新日期：1982-08-31 00:00:00

abstract：:The reactions of metmyoglobin (metMb) and methemoglobin (metHb), oxidized to their respective oxoferryl free radical species (.Mb-FeIV = O/.Hb-4FeIV = O) by tert-butyl hydroperoxide (t-BuOOH), with nitric oxide (NO.) were studied by a combination of optical, electron spin resonance (ESR), ionspray mass (MS), fluoresce...

journal_title：Biochemistry

authors： Gorbunov NV,Osipov AN,Day BW,Zayas-Rivera B,Kagan VE,Elsayed NM

更新日期：1995-05-23 00:00:00

abstract：:Tumor suppressor Nore1, its acronym coming from novel Ras effector, is one of the 10 members of the Rassf (Ras association domain family) protein family that have been identified. It is expressed as two mRNA splice variants, Nore1A and a shorter isoform, Nore1B. It forms homo- and heterocomplexes through its C-termina...

journal_title：Biochemistry

authors： Makbul C,Constantinescu Aruxandei D,Hofmann E,Schwarz D,Wolf E,Herrmann C

更新日期：2013-02-12 00:00:00

abstract：:Chromatography on a column of SP-Sephadex shows that commercial chymopapain contains three components with proteolytic activity. Each behaves as a single protein upon rechromatography and electrophoresis on polyacrylamide gels. The major component, which represents 31% of the activity applied to the column and is the ...

journal_title：Biochemistry

authors： Robinson GW

更新日期：1975-08-12 00:00:00

abstract：:Depletion of Ca2+ and/or Cl- ions from PSII membranes blocks the electron-transfer reactions that precede O2 evolution on the oxidizing side of the enzyme. Illumination of these inhibited preparations at 273 K generates a paramagnetic species that is detectable by low-temperature (T < 20 K) EPR as a signal in the g = ...

journal_title：Biochemistry

authors： Lydakis-Simantiris N,Dorlet P,Ghanotakis DF,Babcock GT

更新日期：1998-05-05 00:00:00

abstract：:In the commonly used nucleation-dependent model of protein aggregation, aggregation proceeds only after a lag phase in which the concentration of energetically unfavorable nuclei reaches a critical value. The formation of oligomeric species prior to aggregation can be difficult to detect by current spectroscopic techn...

journal_title：Biochemistry

authors： Suzuki Y,Brender JR,Soper MT,Krishnamoorthy J,Zhou Y,Ruotolo BT,Kotov NA,Ramamoorthy A,Marsh EN

更新日期：2013-03-19 00:00:00

abstract：:Reactive amyloidosis occurs during chronic inflammation and involves deposition of amyloid A (AA) fibrils in many organs. Amyloid A is derived by proteolysis from serum amyloid A component (SAA), a major acute-phase reactant in many species. Since spontaneous amyloidosis occurs commonly in Syrian hamsters, we have stu...

journal_title：Biochemistry

authors： Webb CF,Tucker PW,Dowton SB

更新日期：1989-05-30 00:00:00

abstract：:Adeno-associated virus (AAV) nonstructural proteins Rep78 and Rep68 carry out all DNA transactions that regulate the AAV life cycle. They share two multifunctional domains: an N-terminal origin binding/nicking domain (OBD) from the HUH superfamily and a SF3 helicase domain. A short linker of ∼20 amino acids that is cr...

journal_title：Biochemistry

authors： Musayev FN,Zarate-Perez F,Bardelli M,Bishop C,Saniev EF,Linden RM,Henckaerts E,Escalante CR

更新日期：2015-09-29 00:00:00

abstract：:To elucidate the enzymatic excision-repair process operative on cyclobutane-type pyrimidine photodimers in human dermal fibroblasts, we have examined excised dimer-containing material recovered in the trichloroacetic acid soluble fraction from far-ultraviolet-irradiated (254 nm, 40 J m-2) and incubated (24 h) cell cul...

journal_title：Biochemistry

authors： Weinfeld M,Gentner NE,Johnson LD,Paterson MC

更新日期：1986-05-06 00:00:00

abstract：:The role of the "C-terminal tail" segment of long neurotoxins has been investigated. The C-terminal four to five residues of alpha-bungarotoxin and Laticauda colubrina b have been cleaved off by carboxypeptidase P. The effect of such deletion on the toxin conformation has been monitored in proton nuclear magnetic reso...

journal_title：Biochemistry

authors： Endo T,Oya M,Tamiya N,Hayashi K

更新日期：1987-07-14 00:00:00

abstract：:Primase is an essential DNA replication enzyme in Escherichia coli and responsible for primer synthesis during lagging strand DNA replication. Although the interaction of primase with single-stranded DNA plays an important role in primer RNA and Okazaki fragment synthesis, the mechanism of DNA binding and site selecti...

journal_title：Biochemistry

authors： Khopde S,Biswas EE,Biswas SB

更新日期：2002-12-17 00:00:00