Comparison of the Forward and Reverse Photocycle Dynamics of Two Highly Similar Canonical Red/Green Cyanobacteriochromes Reveals Unexpected Differences.

abstract：:X-ray reflectivity was used to study the interaction of the PX domain of p40(phox) protein (p40(phox)-PX) with a Langmuir monolayer of a mixture of SOPC (1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine), SOPS (1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine), and DPPtdIns(3)P (1,2-dipalmitoylphosphatidylinositol 3-phos...

journal_title：Biochemistry

authors： Málková S,Stahelin RV,Pingali SV,Cho W,Schlossman ML

更新日期：2006-11-14 00:00:00

abstract：:Chemical modification, mutagenesis, chemical rescue, and isotope effect studies are used to identify and probe the roles of several conserved amino acid groups in catalysis by human dihydroorotate dehydrogenase. Time- and pH-dependent inactivation of human dihydroorotate dehydrogenase by trinitrobenzenesulfonate impli...

journal_title：Biochemistry

authors： Jiang W,Locke G,Harpel MR,Copeland RA,Marcinkeviciene J

更新日期：2000-07-11 00:00:00

abstract：:A new quantitative technique for measuring the binding of proteins to membranes is described. The method is based on a combination of total internal reflection fluorescence microscopy and the preparation of supported planar bilayers. Specific and reversible binding of a fluorescence-labeled monoclonal antibody to lipi...

journal_title：Biochemistry

authors： Kalb E,Engel J,Tamm LK

更新日期：1990-02-13 00:00:00

abstract：:In mature human apolipoprotein A-I (apo A-I), the amino acid residues 1-43 are encoded by exon 3, whereas residues 44-243 are encoded by exon 4 of the apo A-I gene. The region encoded by exon 4 of the apo A-I gene contains 10 tandem amphipathic alpha-helixes; their location and the class to which they belong are as fo...

journal_title：Biochemistry

authors： Mishra VK,Palgunachari MN,Datta G,Phillips MC,Lund-Katz S,Adeyeye SO,Segrest JP,Anantharamaiah GM

更新日期：1998-07-14 00:00:00

abstract：:Molecular cloning revealed the unique serpin endopin 2C that demonstrates selective inhibition of cathepsin L compared to papain or elastase. Endopin 2C, thus, functions as a serpin with the property of cross-class inhibition. Endopin 2C possesses homology in primary sequence to endopin 2A and other isoforms of endopi...

journal_title：Biochemistry

authors： Hwang SR,Stoka V,Turk V,Hook VY

更新日期：2005-05-31 00:00:00

abstract：:Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) is a key element in a signal transduction pathway that couples expression of genes required for glycerol metabolism to the relative availability of glycerol and glucose. Its catalytic activity is inhibited by protein-protein interactions...

journal_title：Biochemistry

authors： Liu WZ,Faber R,Feese M,Remington SJ,Pettigrew DW

更新日期：1994-08-23 00:00:00

abstract：:We have isolated a new toxin, calitoxin (CLX), from the sea anemone Calliactis parasitica whose amino acid sequence differs greatly from that of other sea anemone toxins. The polypeptide chain contains 46 amino acid residues, with a molecular mass of 4886 Da and an isoelectric point at pH 5.4. The amino acid sequence ...

journal_title：Biochemistry

authors： Cariello L,de Santis A,Fiore F,Piccoli R,Spagnuolo A,Zanetti L,Parente A

更新日期：1989-03-21 00:00:00

abstract：:The kinetics of ATP-induced Ca2+ uptake by vesicular dispersions of sarcoplasmic reticulum were determined with a time resolution of about 10 ms, depending on the temperature. Ca2+ uptake was initiated by the addition of ATP through the flash photolysis of P3-1-(2-nitrophenyl)-ethyl adenosine 5'-triphosphate utilizing...

journal_title：Biochemistry

authors： Pierce DH,Scarpa A,Topp MR,Blasie JK

更新日期：1983-11-08 00:00:00

abstract：:In the following, molecular simulations are used to reveal unexpected behavior within bacterial membranes. We show that lipopolysaccharide molecules found in these membranes form viscous amorphous solids when they are interlinked with monovalent and divalent cations. The bilayers exhibit both liquid and glassy charact...

journal_title：Biochemistry

authors： Jefferies D,Hsu PC,Khalid S

更新日期：2017-03-21 00:00:00

abstract：:The substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. This group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. Atu3266 from Agrobacterium tumefaciens C58 and Oant2987 from Ochrobactrum anthropi...

journal_title：Biochemistry

authors： Ornelas A,Korczynska M,Ragumani S,Kumaran D,Narindoshvili T,Shoichet BK,Swaminathan S,Raushel FM

更新日期：2013-01-08 00:00:00

abstract：:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...

journal_title：Biochemistry

authors： Nordell P,Jansson ET,Lincoln P

更新日期：2009-02-24 00:00:00

abstract：:Trypanosomes are purine-auxotrophic parasites that depend upon nucleoside hydrolase (NH) activity to salvage nitrogenous bases necessary for nucleic acid and cofactor synthesis. Nonspecific and purine-specific NHs have been widely studied, yet little is known about the 6-oxopurine-specific isozymes, although they are ...

journal_title：Biochemistry

authors： Vandemeulebroucke A,Minici C,Bruno I,Muzzolini L,Tornaghi P,Parkin DW,Versées W,Steyaert J,Degano M

更新日期：2010-10-19 00:00:00

abstract：:Limited cleavages of human C1r by extrinsic proteases of various specificity (plasmin, elastase, chymotrypsin, thermolysin) yield dimeric associations of two globular domains, each comprised of the intact B chain disulfide linked to gamma, the C-terminal fragment of the A chain. These (gamma-B)2 domains, which are hom...

journal_title：Biochemistry

authors： Arlaud GJ,Gagnon J,Villiers CL,Colomb MG

更新日期：1986-09-09 00:00:00

abstract：:To establish the state of protonation of quinonoid species formed nonenzymically from pyridoxal phosphate (PLP) and diethyl aminomalonate, we have studied absorption spectra of the rapidly established steady-state mixture of species. We have evaluated the formation constant and the spectrum of the mixture of Schiff ba...

journal_title：Biochemistry

authors： Metzler CM,Harris AG,Metzler DE

更新日期：1988-06-28 00:00:00

abstract：:The catalytic activity and inhibitor binding energetics of enzymes are often pH-dependent properties. Aspartic proteases comprise an important class of enzyme targets for structure-based drug design. We have performed a complete thermodynamic study of pepstatin binding to plasmepsin II, an aspartic proteinase found in...

journal_title：Biochemistry

authors： Xie D,Gulnik S,Collins L,Gustchina E,Suvorov L,Erickson JW

更新日期：1997-12-23 00:00:00

abstract：:Despite intense interest in the proteolysis of the β-Amyloid Precursor Protein (APP) in Alzheimer's disease, how the normal processing of this type I receptor-like glycoprotein is physiologically regulated remains ill-defined. In recent years, several candidate protein ligands for APP, including F-spondin, Reelin, β1 ...

journal_title：Biochemistry

authors： Rice HC,Young-Pearse TL,Selkoe DJ

更新日期：2013-05-14 00:00:00

abstract：:Heme propionate-7 in cytochrome c is an ionizable group located in a region of the protein that is inaccessible to bulk solvent. Electrostatic stabilization of this functional group appears to be achieved through interaction of heme propionate-7 with several amino acid residues that occur within hydrogen-bonding dista...

journal_title：Biochemistry

authors： Davies AM,Guillemette JG,Smith M,Greenwood C,Thurgood AG,Mauk AG,Moore GR

更新日期：1993-05-25 00:00:00

abstract：:The allosteric interactions that regulate substrate channeling and catalysis in the tryptophan synthase bienzyme complex from Salmonella typhimurium are triggered by covalent reactions at the beta-site and binding of substrate/product to the alpha-site. The transmission of these allosteric signals between the alpha- a...

journal_title：Biochemistry

authors： Ferrari D,Niks D,Yang LH,Miles EW,Dunn MF

更新日期：2003-07-01 00:00:00

abstract：:New homo- and heterobifunctional cross-linking reagents have been synthesized. These reagents are based on ortho ester, acetal, and ketal functionalities that undergo acid-catalyzed dissociation but are base stable. The protein-reactive group in all the homobifunctional reagents is a maleimide group; the heterobifunct...

journal_title：Biochemistry

authors： Srinivasachar K,Neville DM Jr

更新日期：1989-03-21 00:00:00

abstract：:A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...

journal_title：Biochemistry

authors： Llarrull LI,Mobashery S

更新日期：2012-06-12 00:00:00

abstract：:Retinoschisin (RS1) is a retina-specific secreted protein encoding a conserved discoidin domain sequence. As an adhesion molecule, RS1 preserves the retinal cell architecture and promotes visual signal transduction. In young males, loss-of-function mutations in the X-linked retinoschisis gene (RS1) cause X-linked reti...

journal_title：Biochemistry

authors： Kotova S,Vijayasarathy C,Dimitriadis EK,Ikonomou L,Jaffe H,Sieving PA

更新日期：2010-08-24 00:00:00

abstract：:Restrictocin and related fungal endoribonucleases from the α-sarcin family site-specifically cleave the sarcin/ricin loop (SRL) on the ribosome to inhibit translation and ultimately trigger cell death. Previous studies showed that the SRL folds into a bulged-G motif and tetraloop, with restrictocin achieving a specifi...

journal_title：Biochemistry

authors： Plantinga MJ,Korennykh AV,Piccirilli JA,Correll CC

更新日期：2011-04-12 00:00:00

abstract：:Rh(phen)2phi3+ (phen = 1,10-phenanthroline; phi = 9,10-phenanthrenequinone diimine) avidly binds to DNA via intercalation from the major groove and upon photoactivation produces strand scission with single base 5' asymmetry. Enantiomers of Rh(phen)2phi3+, which lack hydrogen-bonding substituents in ancillary positions...

journal_title：Biochemistry

authors： Campisi D,Morii T,Barton JK

更新日期：1994-04-12 00:00:00

abstract：:The solution conformation of the type I collagen alpha-1 chain N-telopeptide has been studied by CD and 1H NMR spectroscopy at 600 MHz in CD3OH/H2O (60/40 v/v) and H2O solutions. The 19 amino acids form the N-terminal end of the alpha-1 polypeptide chain. By the combined application of several two-dimensional, phase-s...

journal_title：Biochemistry

authors： Otter A,Kotovych G,Scott PG

更新日期：1989-10-03 00:00:00

abstract：:Bovine heart ubiquinol-cytochrome c reductase (bc1 complex) was modified with N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ), which is a selective reagent for buried carboxyl groups. EEDQ treatment caused a loss of the proton pumping activity of liposome-reconstituted bc1 complex, without effect on the passiv...

journal_title：Biochemistry

authors： Cocco T,Di Paola M,Papa S,Lorusso M

更新日期：1998-02-17 00:00:00

abstract：:A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through muta...

journal_title：Biochemistry

authors： Springs SL,Bass SE,McLendon GL

更新日期：2000-05-23 00:00:00

abstract：:Biochemical characterization of the inhibition mechanism of Deltalac-acetogenins synthesized in our laboratory indicated that they are a new type of inhibitor of bovine heart mitochondrial NADH-ubiquinone oxidoreductase (complex I) [Murai, M., et al. (2006) Biochemistry 45, 9778-9787]. To identify the binding site of ...

journal_title：Biochemistry

authors： Kakutani N,Murai M,Sakiyama N,Miyoshi H

更新日期：2010-06-15 00:00:00

abstract：:Staphylococcal fibronectin-binding protein (FnbA) is a surface-associated receptor responsible for the reversible binding of bacteria to human fibronectin and fibrin(ogen). Recently we have shown that FnbA serves as a substrate for coagulation factor XIIIa and undergoes covalent cross-linking to its ligands, resulting...

journal_title：Biochemistry

authors： Anderson ET,Fletcher L,Severin A,Murphy E,Baker SM,Matsuka YV

更新日期：2004-09-21 00:00:00

abstract：:The primary ligand-binding protein (CNTFR alpha) of the multicomponent receptor for ciliary neurotrophic factor was produced in Escherichia coli. Using novel applications of size-exclusion chromatography and a protein gel-shift assay, we obtained quantitative separation of correctly refolded protein, as well as analyt...

journal_title：Biochemistry

authors： Panayotatos N,Everdeen D,Liten A,Somogyi R,Acheson A

更新日期：1994-05-17 00:00:00

abstract：:A novel liposomal method permits studies of Ca movements across the bilayers of multilamellar vesicles (MLV) which had entrapped the Ca-dependent, fluorescent indicator dye Fura 2. Ionomycin-mediated Ca translocation across MLV of phosphatidylcholine (PC)/dicetyl phosphate (DCP), 9:1, obeyed simple first-order kinetic...

journal_title：Biochemistry

authors： Blau L,Weissmann G

更新日期：1988-07-26 00:00:00