Abstract：
:In cerebellum, inositol trisphosphate- (InsP(3)-) gated Ca channels play a key role in learning, though they exhibit a low sensitivity to InsP(3) compared to peripheral tissues. In the present study, the cerebellar InsP(3) receptor is shown to be associated with a novel inhibitor of InsP(3) binding. (3)H-InsP(3) binding studies indicated that this inositol trisphosphate receptor inhibitor (IRI) could completely inhibit InsP(3) binding to the purified cerebellar InsP(3) receptor and acted as a competitive inhibitor. Gel filtration of IRI showed a predominant peak at 6500 Da, though this peak appeared to be an aggregate (with a monomeric molecular mass of approximately 1500 Da). Mass spectrometry of IRI showed a predominant peak at 1635 m/z, consistent with this low molecular mass estimate. The inhibitory activity of IRI was prevented by pretreatment with aryl sulfatase, suggesting the presence of a critical sulfo ester in IRI. IRI was insensitive to proteases and organic extraction but bound to concanavalin A, suggesting that IRI is a sulfated glycan. IRI was present in cerebellum but below the level of detection in aorta. IRI was also present in the neuronal cell line N1E115 (which exhibits a low sensitivity to InsP(3)). We conclude that IRI is a novel endogenous sulfated inhibitor of the InsP(3) receptor that modulates the sensitivity of the InsP(3) receptor and thus may explain the low InsP(3) sensitivity of neurons.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Watras J,Orlando R,Moraru IIdoi
10.1021/bi992175vsubject
Has Abstractpub_date
2000-03-28 00:00:00pages
3452-60issue
12eissn
0006-2960issn
1520-4995pii
bi992175vjournal_volume
39pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The role of interleukin-8 (IL-8) and related CXC chemokines has been demonstrated in many human diseases. However, more profound studies, e.g., by blocking the effect of these inflammatory mediators, request animal models and hence the identification of all human counterparts for commonly used laboratory animals. In t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0011227
更新日期：2000-11-28 00:00:00
abstract：:Hemophores from Serratia marcescens (HasA(sm)) and Pseudomonas aeruginosa (HasA(p)) bind hemin between two loops, which harbor the axial ligands H32 and Y75. Hemin binding to the Y75 loop triggers closing of the H32 loop and enables binding of H32. Because Yersinia pestis HasA (HasA(yp)) presents a Gln at position 32,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400280z
更新日期：2013-04-23 00:00:00
abstract：:The hepatitis delta virus (HDV) ribozyme and HDV-like ribozymes are self-cleaving RNAs found throughout all kingdoms of life. These RNAs fold into a double-nested pseudoknot structure and cleave RNA, yielding 2',3'-cyclic phosphate and 5'-hydroxyl termini. The active site nucleotide C75 has a pK(a) shifted >2 pH units...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100670p
更新日期：2010-08-10 00:00:00
abstract：:Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0481895
更新日期：2004-12-07 00:00:00
abstract：:β-2-Microglobulin (β2m) forms amyloid fibrils in the joints of patients undergoing dialysis treatment as a result of kidney failure. One of the ways in which β2m can be induced to form amyloid fibrils in vitro is via incubation with stoichiometric amounts of Cu(II). To better understand the structural changes caused b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01198
更新日期：2017-02-28 00:00:00
abstract：:Di- and triketone inhibitors of (4-hydroxyphenyl)pyruvate dioxygenase (HPPD) are both effective herbicides and therapeutics. The inhibitory activity is used to halt the production of lipophilic redox cofactors in plants and also in humans to prevent accumulation of toxic metabolic byproducts that arise from specific i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049317s
更新日期：2004-06-01 00:00:00
abstract：:Eukaryotic transfer RNA precursors (pre-tRNAs) contain a 5' leader preceding the aminoacyl acceptor stem and a 3' trailer extending beyond this stem. An early step in pre-tRNA maturation is removal of the 5' leader by the endoribonuclease, RNase P. Extensive pairing between leader and trailer sequences has previously ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000603n
更新日期：2000-08-15 00:00:00
abstract：:Proelastase has been purified to homogeneity from rat pancreatic tissue by a combination of CM-Sephadex and immobilized protease inhibitor affinity resins. Trypsin activation yields an elastolytic enzyme that possesses a specificity toward small hydrophobic residues in synthetic amide substrates, similar to those of p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00285a008
更新日期：1983-08-02 00:00:00
abstract：:The 86-amino acid colicin E9 immunity protein (Im9), which inhibits the DNase activity of colicin E9, has been overexpressed in Escherichia coli and isotopically enriched with 15N and 13C. Using the 3D CBCANH and CBCA(CO)NH experiments, we have almost completely assigned the backbone 13C resonances and extended previo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960401k
更新日期：1996-07-23 00:00:00
abstract：:Transferrins have been extensively studied in order to understand how they reversibly bind and release iron. Human serum transferrin (hTF) is a single polypeptide chain that folds into two lobes (N- and C-lobe); each lobe binds a single ferric ion. Iron release induces a large conformational change in each lobe. At th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602425c
更新日期：2007-09-18 00:00:00
abstract：:We mutated residue 166, located in the putative Na(+) transport pathway between transmembrane segments 4 and 5 of human Na(+)/glucose cotransporter (hSGLT1), from alanine to cysteine (A166C). A166C was expressed in Xenopus laevis oocytes, and electrophysiological methods were used to assay function. The affinity for N...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011026l
更新日期：2001-10-02 00:00:00
abstract：:The temporal evolution of the near-infrared stimulated emission band of the special pair excited state (P*) in the reaction center of Rhodobacter sphaeroides has been studied in intracytoplasmic membranes of the antenna-deficient RCO1 mutant at 10 K with a resolution of 30 fs. On the 100-fs time scale the emission ban...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00188a002
更新日期：1994-06-07 00:00:00
abstract：:Rod outer segment membrane guanylate cyclase (ROS-GC) transduction system is a central component of the Ca(2+)-sensitive phototransduction machinery. The system is composed of two parts: Ca(2+) sensor guanylate cyclase activating protein (GCAP) and ROS-GC. GCAP senses Ca(2+) impulses and inhibits the cyclase. This ope...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034025x
更新日期：2003-05-20 00:00:00
abstract：:hTom20 is an outer mitochondrial membrane receptor involved in protein translocation. The cytosolic domain (aa30-145) and selected truncated versions of this domain were overexpressed and purified to study the structure-function relationship of this protein. Our studies reveal that the secondary structure of the cytos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980746y
更新日期：1998-09-22 00:00:00
abstract：:p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00208a015
更新日期：1994-10-25 00:00:00
abstract：:It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first exampl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00428a046
更新日期：1989-01-24 00:00:00
abstract：:Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00629a014
更新日期：1977-05-17 00:00:00
abstract：:Each homologous lobe of human serum transferrin (hTF) has one Fe(3+) ion bound by an aspartic acid, a histidine, two tyrosine residues, and two oxygens from the synergistic anion, carbonate. Extensive characterization of these ligands in the N-terminal lobe has been carried out. Despite sharing the same set of ligands...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050015f
更新日期：2005-06-07 00:00:00
abstract：:Actinaria cytolysins are very potent basic toxins isolated from the venom of sea anemones, which are supposed to exert their toxic activity through formation of oligomeric pores in the host plasma membrane. To gain insight into their mechanism of action, the interaction of Stichodactyla helianthus sticholysin I (St-I)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960787z
更新日期：1996-11-26 00:00:00
abstract：:The structure of isocitrate dehydrogenase (IDH) with a bound complex of isocitrate, NADP+, and Ca2+ was solved at 2.5-A resolution and compared by difference mapping against previously determined enzymatic complexes. Calcium replaces magnesium in the binding of metal-substrate chelate complex, resulting in a substanti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00087a008
更新日期：1993-09-14 00:00:00
abstract：:Resonance Raman spectroscopy has been used to study milk xanthine oxidase, an enzyme containing molybdenum, binuclear iron-sulfur clusters, and FAD as cofactors. The contribution of FAD dominates the resonance Raman spectrum at frequencies above 500 cm-1. As expected, no bands assignable to FAD are observed in deflavo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00332a026
更新日期：1985-05-21 00:00:00
abstract：:The structural and functional properties of G protein-coupled receptors (GPCRs) are often studied in a detergent micellar environment, but many GPCRs tend to denature or aggregate in short alkyl chain detergents. In our previous work [Lee, S., et al. (2016) J. Am. Chem. Soc.138, 15425-15433], we showed that GPCRs in a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00183
更新日期：2020-06-16 00:00:00
abstract：:Polyclonal antibody to mitochondrial P-450c27/25 reacted with two proteins of apparent molecular masses of 52 kilodaltons (kDa) and 50 kDa from the female rat liver mitochondrial proteins bound to an omega-octylaminoagarose column. The two proteins were purified to greater than 85% homogeneity by DEAE-Sephacel and hyd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00098a007
更新日期：1991-08-27 00:00:00
abstract：:A general kinetic model for catalysis by interfacial enzymes is developed. It couples the Michaelis-Menten catalytic turnover cycle at the interface with that in the aqueous phase through the distribution equilibria between the interface and the surrounding aqueous phase. Analysis under two limiting conditions fully d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970855x
更新日期：1997-11-25 00:00:00
abstract：:The structure of empty capsids of Kilham rat virus, an autonomous parvovirus with icosahedral symmetry, was investigated by small-angle neutron scattering. From the forward scatter, the molecular weight was determined to be 4.0 X 10(6), and from the Guinier region, the radius of gyration was found to be 105 A in D2O a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00321a044
更新日期：1984-12-18 00:00:00
abstract：:p-Hydroxyphenylpyruvate dioxygenase (HPD) plays a key role in the normal catabolism of tyrosine. An Fe2+/oxygen-dependent enzyme, it converts p-hydroxyphenylpyruvate into homogentisate and is part of the superfamily of alpha-ketoglutarate-dependent enzymes that couples oxidative decarboxylation of an alpha-ketoacid co...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035762w
更新日期：2004-01-27 00:00:00
abstract：:The clathrin assembly protein complex AP-2 is a multimeric subunit complex consisting of two 100-115-kDa subunits known as alpha and beta and 50- and 16-kDa subunits. The subunits have been dissociated and separated by ion-exchange chromatography in 7.5 M urea. Fractions highly enriched in either the alpha or beta sub...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00236a036
更新日期：1991-06-04 00:00:00
abstract：:The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100055m
更新日期：2010-05-11 00:00:00
abstract：:Previously, we have shown that 4-hydroxynonenal (4-HNE) induces Fas-mediated apoptosis in HLE B-3 cells through a pathway which is independent of FasL, FADD, procaspase 8, and DISC (Li, J., et al. (2006) Biochemistry 45, 12253-12264). The involvement of Daxx has also been suggested in this pathway, but its role is not...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701559f
更新日期：2008-01-08 00:00:00
abstract：:We have investigated, both experimentally and theoretically, the efflux of carboxyfluorescein (a self-quenching fluorescent dye) from vesicles of different sizes and lipid species (POPC, DOPC) after having added the bee venom peptide melittin. This comprises quantitative analyses regarding the extent of lipid-associat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971009p
更新日期：1998-02-24 00:00:00
