Isolation and characterization of rat pancreatic elastase.

abstract：:Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...

journal_title：Biochemistry

authors： Czekster CM,Naismith JH

更新日期：2017-04-18 00:00:00

abstract：:Macrophage migration inhibitory factor (MIF) is a pro-inflammatory and tumor-promoting cytokine that occurs in two redox-dependent immunologically distinct conformational isoforms. The disease-related structural isoform of MIF (oxMIF) can be specifically and predominantly detected in the circulation of patients with i...

journal_title：Biochemistry

authors： Schinagl A,Kerschbaumer RJ,Sabarth N,Douillard P,Scholz P,Voelkel D,Hollerweger JC,Goettig P,Brandstetter H,Scheiflinger F,Thiele M

更新日期：2018-03-06 00:00:00

abstract：:Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(II) dependent agmatinase (SpeB). This is the first report of an iron-dependent agmatinase. We demonstrate that aerobically isolated recombinant enzyme contains two disulfide bonds and only a trace amount of any metal and...

journal_title：Biochemistry

authors： Miller D,Xu H,White RH

更新日期：2012-04-10 00:00:00

abstract：:For nonspecific binding of oligopeptides and other cationic ligands, including proteins, to nucleic acid oligomers, we develop a model capable of quantifying and predicting the salt concentration dependence of the binding free energy (deltaG(o)obs) by way of an analytic treatment of the Coulombic end effect (CEE). Lig...

journal_title：Biochemistry

authors： Shkel IA,Ballin JD,Record MT Jr

更新日期：2006-07-11 00:00:00

abstract：:Enolase is a glycolytic metalloenzyme involved in carbon metabolism. The advantage of targeting enolase lies in its essentiality in many biological processes such as cell wall formation and RNA turnover and as a plasminogen receptor. We initially used a DARTS assay to identify enolase as a target in Escherichia coli. ...

journal_title：Biochemistry

authors： Krucinska J,Falcone E,Erlandsen H,Hazeen A,Lombardo MN,Estrada A,Robinson VL,Anderson AC,Wright DL

更新日期：2019-03-05 00:00:00

abstract：:The luminescence properties (fluorescence and phosphorescence) of luliberin have been investigated at liquid-nitrogen temperature (77 K) in 50% ethylene glycolaqueous buffer at various pH's. Calculation of the energy-transfer efficiency between Trp and Tyr residues leads to an evaluation of the distance separating the...

journal_title：Biochemistry

authors： Marche P,Montenay-Garestier T,Fromageot P,Hélène C

更新日期：1976-12-28 00:00:00

abstract：:Although the Escherichia coli fatty acid synthesis (FAS) pathway is the best studied type II fatty acid synthesis system, a major experimental limitation has been the inability to feed intermediates into the pathway in vivo because exogenously supplied free fatty acids are not efficiently converted to the acyl-acyl ca...

journal_title：Biochemistry

authors： Jiang Y,Morgan-Kiss RM,Campbell JW,Chan CH,Cronan JE

更新日期：2010-02-02 00:00:00

abstract：:Proteins denature at low pH because of intramolecular electrostatic repulsions. The addition of salt partially overcomes this repulsion for some proteins, yielding a collapsed conformation called the A-state. A-states have characteristics expected for the molten globule, a notional kinetic protein folding intermediate...

journal_title：Biochemistry

authors： Davis-Searles PR,Morar AS,Saunders AJ,Erie DA,Pielak GJ

更新日期：1998-12-01 00:00:00

abstract：:RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell prolif...

journal_title：Biochemistry

authors： Wang X,Ge W,Zhang Y

更新日期：2019-01-29 00:00:00

abstract：:The pathogenic bacteria Pseudomonas aeruginosa and Bordetella pertussis contain in their outer membranes the rare sugar 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid. Five enzymes are required for the biosynthesis of this sugar starting from UDP-N-acetylglucosamine. One of these, referred to as WlbB, is an N-acetyltra...

journal_title：Biochemistry

authors： Thoden JB,Holden HM

更新日期：2010-06-08 00:00:00

abstract：:The duplex formed by annealing the formacetal backbone modified dodecamer d-(CGCGTTOCH2OTTGCGC) to its complementary strand, d(GCGCAAAACGCG) (duplex I), has been studied by NMR techniques and analyzed with reference to its unmodified counterpart (duplex II). Comparison of parameters such as 2D cross-peak intensities, ...

journal_title：Biochemistry

authors： Gao X,Brown FK,Jeffs P,Bischofberger N,Lin KY,Pipe AJ,Noble SA

更新日期：1992-07-14 00:00:00

abstract：:Glucocorticoid receptors of wild-type and nti ("increased nuclear transfer") mutant S49.1 mouse lymphoma cells exist in extracts under low-salt conditions predominantly as high molecular weight species (Mr greater than or equal to 300,000). These receptor-hormone complexes are unable to bind to DNA. High salt (300 mM ...

journal_title：Biochemistry

authors： Rexin M,Busch W,Gehring U

更新日期：1988-07-26 00:00:00

abstract：:Cdx and Hox gene families encode homeodomain-containing transcription factors involved in anterior-posterior vertebral patterning. Although Cdx proteins are direct transcriptional regulators of Hox gene expression, both Hox and Cdx proteins are known to interact with other homeodomain transcription factors, leading us...

journal_title：Biochemistry

authors： Lafontaine CA,Grainger S,Hess BL,Béland M,Lohnes D

更新日期：2012-12-04 00:00:00

abstract：:In reconstituted vesicles above the lipid phase transition temperature, bacteriorhodopsin (BR) undergoes rotational diffusion about an axis perpendicular to the plane of the bilayer [Cherry, R. J., Muller, U., & Schneider, G. (1977) FEBS Lett. 80, 465]. This diffusion narrows the 13C NMR powder line shape of the BR pe...

journal_title：Biochemistry

authors： Lewis BA,Harbison GS,Herzfeld J,Griffin RG

更新日期：1985-08-13 00:00:00

abstract：:All retroviruses package two copies of their genomes during virus assembly, both of which are required for strand transfer-mediated recombination during reverse transcription. Genome packaging is mediated by interactions between the nucleocapsid (NC) domains of assembling Gag polyproteins and an RNA packaging signal, ...

journal_title：Biochemistry

authors： Dey A,York D,Smalls-Mantey A,Summers MF

更新日期：2005-03-15 00:00:00

abstract：:We extended the technique of frequency-domain fluorometry to an upper frequency limit of 2000 MHz. This was accomplished by using the harmonic content of a laser pulse train (3.76 MHz, 5 ps) from a synchronously pumped and cavity-dumped dye laser. We used a microchannel plate photomultiplier as the detector to obtain ...

journal_title：Biochemistry

authors： Lakowicz JR,Laczko G,Gryczynski I

更新日期：1987-01-13 00:00:00

abstract：:Here, we introduce the concept of the "seleno effect" in the study of oxidoreductases that catalyze thiol/disulfide exchange reactions. In these reactions, selenium can replace sulfur as a nucleophile, electrophile, or leaving group, and the resulting change in rate (the seleno effect) is defined as kS/ kSe. In soluti...

journal_title：Biochemistry

authors： O'Keefe JP,Dustin CM,Barber D,Snider GW,Hondal RJ

更新日期：2018-03-20 00:00:00

abstract：:Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formati...

journal_title：Biochemistry

authors： Chi LM,Lam SL

更新日期：2009-12-08 00:00:00

abstract：:Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone involved in the maintenance of the corpus luteum in early pregnancy. The free alpha-subunit of hCG has a biological activity of its own, namely, stimulation of prolactin secretion from term pregnancy decidual cells [Blithe, D. L., et al. (1991)...

journal_title：Biochemistry

authors： van Zuylen CW,de Beer T,Leeflang BR,Boelens R,Kaptein R,Kamerling JP,Vliegenthart JF

更新日期：1998-02-17 00:00:00

abstract：:p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine o...

journal_title：Biochemistry

authors： Koyama T,Obata S,Saito K,Takeshita-Koike A,Ogura K

更新日期：1994-10-25 00:00:00

abstract：:Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Freier SM,Turner DH

更新日期：1986-09-23 00:00:00

abstract：:The Y70F mutant of aspartate aminotransferase has reduced affinity for coenzymes compared to the wild type. The equilibrium dissociation constants for pyridoxamine phosphate (PMP) holoenzymes, KPMPdiss, were determined from the association and dissociation rate constants to be 1.3 nM and 30 nM for the wild type and mu...

journal_title：Biochemistry

authors： Toney MD,Kirsch JF

更新日期：1991-07-30 00:00:00

abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...

journal_title：Biochemistry

authors： Kasson PM,Rabinowitz JD,Schmitt L,Davis MM,McConnell HM

更新日期：2000-02-08 00:00:00

abstract：:Pyrimidine oligonucleotides have been shown to prime the E. coli DNA polymerase I repair reaction, specifically and reproducibly. DNA molecules up to 30 nucleotides long have been obtained from the extension of oligopyrimidine primers, 9 to 11 nucleotides long isolated from the complementary (minus) strand of bacterio...

journal_title：Biochemistry

authors： Kaptein JS,Spencer JH

更新日期：1978-03-07 00:00:00

abstract：:Living organisms have evolved a vast array of catalytic functions that make them ideally suited for the production of medicinally and industrially relevant small-molecule targets. Indeed, native metabolic pathways in microbial hosts have long been exploited and optimized for the scalable production of both fine and co...

journal_title：Biochemistry

authors： Weeks AM,Chang MC

更新日期：2011-06-21 00:00:00

abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...

journal_title：Biochemistry

authors： Sha R,Liu F,Iwasaki H,Seeman NC

更新日期：2002-09-10 00:00:00

abstract：:Animal galectins (lectins with specificity for beta-galactosides of glycan chains) are potent effectors in diverse aspects of cell sociology. Gene divergence has led to different groups and a marked interspecies variability in the number of members per group. Since the suitability of a model for studying functionality...

journal_title：Biochemistry

authors： Kaltner H,Solís D,André S,Lensch M,Manning JC,Mürnseer M,Sáiz JL,Gabius HJ

更新日期：2009-05-26 00:00:00

abstract：:While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...

journal_title：Biochemistry

authors： Xin X,Xi L,Tu SC

更新日期：1994-10-11 00:00:00

abstract：:Treatment of human lymphoid cells with methyl methanesulfonate (MMS) results in discontinuities in cellular DNA. Discontinuities can be detected by sedimentation through neutral sucrose gradients after digestion with S1 nuclease on top of the gradient and by benzoylated naphthoylated diethylaminoethylcellulose (BND-ce...

journal_title：Biochemistry

authors： Karran P,Higgins NP,Strauss B

更新日期：1977-10-04 00:00:00

abstract：:Most terpene synthase reactions follow Markovnikov rules for formation of high-energy carbenium ion intermediates. However, there are notable exceptions. For example, pentalenene synthase (PS) undergoes an initial anti-Markovnikov cyclization reaction followed by a 1,2-hydride shift to form an intermediate humulyl cat...

journal_title：Biochemistry

authors： Matos JO,Kumar RP,Ma AC,Patterson M,Krauss IJ,Oprian DD

更新日期：2020-09-08 00:00:00