Molecular structure of WlbB, a bacterial N-acetyltransferase involved in the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid .

abstract：:The activity of peptidyl-tRNALys-CpCp2'dA was measured in an in vitro poly(A)-dependent polypeptide synthesizing system derived from Escherichia coli. It has already been shown that Lys-tRNALys-CpCp2'dA is active as an acceptor and Ac2-Lys-tRNALys-Cp2'dA can donate its peptidyl residue but that the overall poly(A)-dep...

journal_title：Biochemistry

authors： Wagner T,Sprinzl M

更新日期：1983-01-04 00:00:00

abstract：:The relationship between GTP hydrolysis and microtubule assembly has been investigated by using a rapid filtration method. Microtubules assembled from phosphocellulose-purified tubulin, double-labeled with [gamma-32P]- and [3H]GTP, were trapped and washed free of unbound nucleotide on glass fiber filters. The transien...

journal_title：Biochemistry

authors： Stewart RJ,Farrell KW,Wilson L

更新日期：1990-07-10 00:00:00

abstract：:The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second doe...

journal_title：Biochemistry

authors： Ha-Duong NT,Eid C,Hémadi M,El Hage Chahine JM

更新日期：2010-12-07 00:00:00

abstract：:The downfield (9-15 ppm) proton spectrum of Escherichia coli 5S RNA has been examined at 500 MHz by using nuclear Overhauser methods. The data confirm the existence of the terminal and procaryotic loop helices within the molecule [Fox, G. E., & Woese, C. R. (1975) Nature (London) 256, 505-506]. Very little stable, dou...

journal_title：Biochemistry

authors： Kime MJ,Moore PB

更新日期：1983-05-24 00:00:00

abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...

journal_title：Biochemistry

authors： Miao R,Martinho M,Morales JG,Kim H,Ellis EA,Lill R,Hendrich MP,Münck E,Lindahl PA

更新日期：2008-09-16 00:00:00

abstract：:The kinetic parameters (kpol, Kd app) for all possible correct and incorrect pairing between the A, T, G, and C bases were determined for wild-type (WT) rat DNA polymerase beta (pol beta) and the R283A mutant under pre-steady-state kinetic assay conditions. The base substitution fidelities of these two proteins were t...

journal_title：Biochemistry

authors： Ahn J,Werneburg BG,Tsai MD

更新日期：1997-02-04 00:00:00

abstract：:Although there is considerable evidence that signaling by the erythropoietin (EPO) receptor is initiated when it is dimerized by binding EPO, it has been previously reported that the soluble extracellular domains of the EPO receptor (sEPOR) are not dimerized in the presence of EPO and are able to form only 1:1 complex...

journal_title：Biochemistry

authors： Philo JS,Aoki KH,Arakawa T,Narhi LO,Wen J

更新日期：1996-02-06 00:00:00

abstract：:Thioredoxin reductase from Escherichia coli is a member of the pyridine nucleotide-disulfide oxidoreductase family, and contains one FAD and one redox-active disulfide per subunit. It is known that two other well-studied members of this family, lipoamide dehydrogenase and glutathione reductase, cycle between the two e...

journal_title：Biochemistry

authors： Lennon BW,Williams CH Jr

更新日期：1996-04-16 00:00:00

abstract：:A mechanistic study of rat liver alpha-(2-->6) sialyltransferase (ST) is presented that includes isotope trapping experiments and kinetic isotope effects on V/K for the ST-catalyzed reaction of isotopically labeled CMP-N-acetylneuraminate and N-acetyllactosamine. The isotope trapping experiments confirmed that the kin...

journal_title：Biochemistry

authors： Bruner M,Horenstein BA

更新日期：1998-01-06 00:00:00

abstract：:The 77-amino acid pro-domain greatly accelerates the in vitro folding of subtilisin in a bimolecular reaction whose product is a tight complex between folded subtilisin and folded pro-domain. In this complex the pro-domain has a compact structure with a four-stranded antiparallel beta-sheet and two three-turn alpha-he...

journal_title：Biochemistry

authors： Wang L,Ruan B,Ruvinov S,Bryan PN

更新日期：1998-03-03 00:00:00

abstract：:Three electrochromic difference spectra induced by the deposition of (1) a negative charge on the primary quinone acceptor, Q(A), (2) a positive charge on (or near) Tyr161 of the D1 subunit (Y(Z)), and (3) a positive charge on the manganese cluster were determined at room temperature in photosystem II (PSII) core part...

journal_title：Biochemistry

authors： Mulkidjanian AY,Cherepanov DA,Haumann M,Junge W

更新日期：1996-03-05 00:00:00

abstract：:Several common links between the structural chemistry of the chitolipooligosaccharides of Rhizobium and the general rhizobial membrane lipid and lipopolysaccharide chemistry of these bacteria have been uncovered. Aspects of common chemistry include sulfation, methylation, and the position and extent of fatty acyl chai...

journal_title：Biochemistry

authors： Cedergren RA,Lee J,Ross KL,Hollingsworth RI

更新日期：1995-04-04 00:00:00

abstract：:Incorporation of nonproteinogenic amino acids in small polypeptides synthesized by nonribosomal peptide synthetases (NRPS) significantly contributes to their biological activity. In these peptides, conversion of L-amino acids to the corresponding D-isomer is catalyzed by specialized NRPS modules that utilize an epimer...

journal_title：Biochemistry

authors： Linne U,Doekel S,Marahiel MA

更新日期：2001-12-25 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:An assay was developed to study the spontaneous transfer and transbilayer movement (flip-flop) of lipid analogs labeled with the fluorescent fatty acid, 5-(5,7-dimethyl BODIPY)-1-pentanoic acid (C5-DMB-) in large unilamellar lipid vesicles comprised of 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC). The assay is base...

journal_title：Biochemistry

authors： Bai J,Pagano RE

更新日期：1997-07-22 00:00:00

abstract：:Protocatechuate 3,4-dioxygenase (3,4-PCD) utilizes a ferric ion to catalyze the aromatic ring cleavage of 3,4-dihydroxybenzoate (PCA) by incorporation of both atoms of dioxygen to yield beta-carboxy-cis, cis-muconate. The crystal structures of the anaerobic 3,4-PCD.PCA complex, aerobic complexes with two heterocyclic ...

journal_title：Biochemistry

authors： Orville AM,Lipscomb JD,Ohlendorf DH

更新日期：1997-08-19 00:00:00

abstract：:Phase-sensitive 2D 1H/1H COSY spectra can be used to identify the structures of individual pure specimens of the aminoglycoside antibiotic amikacin and its N-hemisuccinyl derivatives. However, even at 500 MHz the 2D chemical shift dispersion does not allow for unambiguous assignment of all cross-peaks. By use of 2D re...

journal_title：Biochemistry

authors： Andersen NH,Eaton HL,Nguyen KT,Hartzell C,Nelson RJ,Priest JH

更新日期：1988-04-19 00:00:00

abstract：:4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolysis of 4-CBA-CoA to 4-hydroxybenzoyl-coenzyme A (4-HBA-CoA) via a nucleophilic aromatic substitution pathway involving the participation of an active site carboxylate side chain in covalent catalysis. In this paper we report on the identification...

journal_title：Biochemistry

authors： Yang G,Liu RQ,Taylor KL,Xiang H,Price J,Dunaway-Mariano D

更新日期：1996-08-20 00:00:00

abstract：:Enolase in the presence of Mg2+ catalyzes the elimination of H2O from 2-phosphoglyceric acid (PGA) to form phosphoenolpyruvate (PEP) and the reverse reaction, the hydration of PEP to PGA. The structure of the ternary complex yeast enolase-Mg2(+)-PGA/PEP has been determined by X-ray diffraction and refined by crystallo...

journal_title：Biochemistry

authors： Lebioda L,Stec B

更新日期：1991-03-19 00:00:00

abstract：:Urease from Klebsiella aerogenes is composed of three subunits (UreA-UreB-UreC) that assemble into a (UreABC)(3) quaternary structure. UreC harbors the dinuclear nickel active site, whereas the functions of UreA and UreB remain unknown. UreD and UreF accessory proteins previously were suggested to reposition UreB and ...

journal_title：Biochemistry

authors： Carter EL,Boer JL,Farrugia MA,Flugga N,Towns CL,Hausinger RP

更新日期：2011-11-01 00:00:00

abstract：:Novel inhibitors of lupin diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap(4)A) hydrolase have been identified by in silico screening of a large virtual chemical library. Compounds were ranked on the basis of a consensus from six scoring functions. From the top 100 ranked compounds six were selected and initially scre...

journal_title：Biochemistry

authors： Branson KM,Mertens HD,Swarbrick JD,Fletcher JI,Kedzierski L,Gayler KR,Gooley PR,Smith BJ

更新日期：2009-08-18 00:00:00

abstract：:Glucocorticoid (GC) hormones induce apoptosis in T-cell and pre-B-cell acute lymphoblastic leukemia (ALL) cells. Steroid-mediated apoptosis requires a threshold level of the glucocorticoid receptor (GR) protein, and increasing the intracellular GR levels in ALL cells would augment their hormone sensitivity. A protein ...

journal_title：Biochemistry

authors： Geng CD,Vedeckis WV

更新日期：2010-10-19 00:00:00

abstract：:NAD(P)H:quinone oxidoreductase 1 (NQO1) is currently an emerging target in pancreatic cancer. In this report, we describe a series of indolequinones, based on 5-methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione (ES936), and evaluate NQO1 inhibition and growth inhibitory activity in the human pancreatic M...

journal_title：Biochemistry

authors： Reigan P,Colucci MA,Siegel D,Chilloux A,Moody CJ,Ross D

更新日期：2007-05-22 00:00:00

abstract：:We have labeled the primer binding domain of murine leukemia virus reverse transcriptase (MuLV RT) by covalently cross-linking 5' end labeled d(T)8 to MuLV RT, using ultraviolet light energy. The specificity and the functional significance of the primer cross-linking reaction were demonstrated by the fact that (i) oth...

journal_title：Biochemistry

authors： Tirumalai RS,Modak MJ

更新日期：1991-07-02 00:00:00

abstract：:c-Myc is a member of a family of sequence specific-DNA binding proteins that are thought to regulate the transcription of genes involved in normal cell growth, differentiation, and apoptosis. In order to understand how human c-myc functions as a transcription factor, we have studied the mechanism of action and structu...

journal_title：Biochemistry

authors： McEwan IJ,Dahlman-Wright K,Ford J,Wright AP

更新日期：1996-07-23 00:00:00

abstract：:Glutamate mutase (GM) is a cobalamin-dependent enzyme that catalyzes the reversible interconversion of L-glutamate and L-threo-3-methylaspartate via a radical-based mechanism. To initiate catalysis, the 5'-deoxyadenosylcobalamin (AdoCbl) cofactor's Co-C bond is cleaved homolytically to generate an adenosyl radical and...

journal_title：Biochemistry

authors： Brooks AJ,Fox CC,Marsh EN,Vlasie M,Banerjee R,Brunold TC

更新日期：2005-11-22 00:00:00

abstract：:One of the putative proton-transfer pathways leading from solution toward the binuclear center in many cytochrome c oxidases is the D-pathway, so-called because it starts with a highly conserved aspartate [D(I-132)] residue. Another highly conserved amino acid residue in this pathway, glutamate(I-286), has been indica...

journal_title：Biochemistry

authors： Aagaard A,Gilderson G,Mills DA,Ferguson-Miller S,Brzezinski P

更新日期：2000-12-26 00:00:00

abstract：:Protein kinase C and two other proteins with molecular masses of 64 and 32 kDa, purified from bovine brain, constitute a type of protein that binds a large number of calcium ions in a phospholipid-dependent manner. This study suggested that these proteins also induced extensive clustering of acidic phospholipids in th...

journal_title：Biochemistry

authors： Bazzi MD,Nelsestuen GL

更新日期：1991-08-13 00:00:00

abstract：:The evolutionarily conserved DRY motif at the end of the third helix of rhodopsin-like, class-A G protein-coupled receptors (GPCRs) is a major regulator of receptor stability, signaling activity, and β-arrestin-mediated internalization. Substitution of the DRY arginine with histidine in the human vasopressin receptor ...

journal_title：Biochemistry

authors： Burczyk M,Burkhalter MD,Blätte T,Matysik S,Caron MG,Barak LS,Philipp M

更新日期：2015-01-27 00:00:00

abstract：:The two most basic charge isomers of myelin basic protein (BP), components 1 and 2 (C1 and C2), which presumably differ in the degree of deamidation, were purified from bovine BP by cation-exchange chromatography. Two additional specific types of posttranslational modifications were introduced into the purified isomer...

journal_title：Biochemistry

authors： Cheifetz S,Boggs JM,Moscarello MA

更新日期：1985-09-10 00:00:00