Abstract：
:The two most basic charge isomers of myelin basic protein (BP), components 1 and 2 (C1 and C2), which presumably differ in the degree of deamidation, were purified from bovine BP by cation-exchange chromatography. Two additional specific types of posttranslational modifications were introduced into the purified isomers: (1) C-terminal arginine deficient derivatives of C1 and C2 were prepared by incubating the isomers with a carboxypeptidase, and (2) phosphorylated derivatives of C1 (1.6 and 1.7 mol of phosphate/mol of protein) were prepared by incubating C1 with the protein kinase from rabbit muscle. The ability of these charge isomers to increase the permeability of multilamellar vesicles composed of phosphatidylserine/phosphatidylcholine (1:11.5 w/w) and sphingomyelin/cholesterol/phosphatidic acid (1:1:0.2 w/w/w) was measured by monitoring the release of a water-soluble spin-label (tempocholine chloride) from the vesicles. The increase in vesicle permeability caused by BP was taken as a measure of the degree of perturbation of the bilayer by the protein, most likely by penetration partly into the bilayer. All classes of charge isomers (naturally occurring or generated in vitro) were more effective at increasing vesicle permeability than was poly(L-lysine), a polycation that only interacts electrostatically with the bilayer. Although C1 and C2 and their C-terminal-deficient derivatives did not differ in the amount of marker released, the phosphorylated derivative of C1 caused a smaller increase in vesicle permeability than did the other isomers, suggesting that phosphorylation had altered the ability of the protein to perturb the bilayer.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Cheifetz S,Boggs JM,Moscarello MAdoi
10.1021/bi00340a032subject
Has Abstractpub_date
1985-09-10 00:00:00pages
5170-5issue
19eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The beta 2-adrenergic receptor (beta 2AR) is a member of a large superfamily of seven transmembrane domain, G-protein-coupled receptors. Within the putative seventh transmembrane domain of the beta 2AR is a sequence of amino acids, NPLIY, which is conserved with minor variations in all members of the superfamily. Prev...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00047a003
更新日期：1995-11-28 00:00:00
abstract：:Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterizat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00496a011
更新日期：1990-11-06 00:00:00
abstract：:It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00167a038
更新日期：1994-01-11 00:00:00
abstract：:The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051316s
更新日期：2005-11-08 00:00:00
abstract：:The functional interaction of the recombinant alpha 2 adrenergic receptor subtypes, alpha 2-C10 (the human platelet alpha 2 receptor, equivalent to the alpha 2 A subtype) and alpha 2-C4 (an alpha 2 receptor subtype cloned from a human kidney cDNA library), with G proteins was characterized in an in vitro reconstitutio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00227a024
更新日期：1991-04-02 00:00:00
abstract：:Myocardial contraction is initiated when Ca2+ binds to site II of cardiac troponin C. This 12-residue EF-hand loop (NH2-DEDGSGTVDFDE-COOH) contains six residues (bold) that coordinate Ca2+ binding and six residues that do not appear to influence Ca2+ binding directly. We have introduced six single-cysteine substitutio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800164c
更新日期：2008-05-06 00:00:00
abstract：:The interactions of mandelate racemase with divalent metal ion, substrate, and competitive inhibitors were investigated. The enzyme was found by electron paramagnetic resonance (EPR) to bind 0.9 Mn2+ ion per subunit with a dissociation constant of 8 muM, in agreement with its kinetically determined activator constant....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00677a006
更新日期：1975-03-25 00:00:00
abstract：:UDP-glucose:glycoprotein glucosyltransferase (UGT) is a soluble protein of the endoplasmic reticulum (ER) that operates as a gatekeeper for quality control by preventing transport of improperly folded glycoproteins out of the ER. We report the isolation of two cDNAs encoding human UDP-glucose:glycoprotein glucosyltran...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9916473
更新日期：2000-03-07 00:00:00
abstract：:ATP and the fluorescent substrate analogue TNP-ATP bind competitively to the gastric H,-K-ATPase. Substrate and product completely reverse the fluorescence enhancement caused by TNP-ATP binding to the enzyme. The fluorophore is displaced monophasically from apoenzyme. However, ATP displaces TNP-ATP from the Mg2+-quenc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00442a034
更新日期：1989-08-08 00:00:00
abstract：:The NO adducts of leghemoglobin (Lb) are implicated in biological processes, but only the adduct with ferrous Lb (Lb(II)NO) has been characterized previously. We report the first characterization of ferric nitrosylleghemoglobin (Lb(III)NO) and XAS experiments performed on frozen aqueous solutions of Lb(II)NO and Lb(II...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990730n
更新日期：1999-12-14 00:00:00
abstract：:The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, together with conventional NOE constraints. The use of pseudocontact shift constraints ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971022+
更新日期：1997-09-30 00:00:00
abstract：:Autophagy, an essential eukaryotic homeostasis pathway, allows the sequestration of unwanted, damaged, or harmful cytoplasmic components in vesicles called autophagosomes, permitting subsequent lysosomal degradation and nutrient recycling. Autophagosome nucleation is mediated by class III phosphatidylinositol-3-kinase...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00246
更新日期：2016-08-02 00:00:00
abstract：:Kinetic analysis and modeling studies of HIV-1 and HIV-2 proteinases were carried out using the oligopeptide substrate [formula: see text] and its analogs containing single amino acid substitutions in P3-P3' positions. The two proteinases acted similarly on the substrates except those having certain hydrophobic amino ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00135a008
更新日期：1992-05-26 00:00:00
abstract：:We find that specific oxidation for the Met-192 residue in delta-chymotrypsin to methionine sulfoxide results in a twofold increase in Km(app) and unchanged kcat in the hydrolysis of N-acetyl mono(amino acid) amide substrates. However, the catalyzed hydrolyses of N-acetyl dipeptide amide substrates by (methionine sulf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a006
更新日期：1976-09-21 00:00:00
abstract：:We have used DNase I footprinting to examine the formation of antiparallel DNA triple helices on DNA fragments containing the homopurine target sites (GGA)2GGX(GGA)2GG.(CCT)2CCZ(CCT)2CC (where X.Z is each base pair in turn), with the GA- and GT-rich oligonucleotides, (GGA)2GGN(GGA)2GG and (GGT)2GGN(GGT)2GG (N = each b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9609679
更新日期：1996-11-26 00:00:00
abstract：:Binding of deoxyribonucleoside 5'-triphosphates to DNA polymerase I of Escherichia coli was measured by using a microscale nonequilibrium dialysis method. It allowed rapid and economic measurement of dissociation constants, with negligible interfering side reactions. A stoichiometry of 1 mol of nucleoside 5'-triphosph...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00335a033
更新日期：1985-07-02 00:00:00
abstract：:The crystal structure of the complex between the quinoprotein methylamine dehydrogenase (MADH) and the type I blue copper protein amicyanin, both from Paracoccus denitrificans, has been determined at 2.5-A resolution using molecular replacement. The search model was MADH from Thiobacillus versutus. The amicyanin could...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00136a006
更新日期：1992-06-02 00:00:00
abstract：:The interaction between the calcium binding and inhibitory components of troponin is central to the regulation of muscle contraction. In this work, two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance (2D-¿1H,15N¿-HSQC NMR) spectroscopy was used to determine the stoichiometry, affinity, an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992579n
更新日期：2000-03-21 00:00:00
abstract：:Backbone amide dynamics of the Escherichia coli tryptophan repressor protein (WT-TrpR) and two functionally distinct variants, L75F-TrpR and A77V-TrpR, in their holo (l-tryptophan corepressor-bound) form have been characterized using (15)N nuclear magnetic resonance (NMR) relaxation. The three proteins possess very si...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200389k
更新日期：2011-06-14 00:00:00
abstract：:The ferlin family proteins have emerged as multi-C2 domain regulators of calcium-triggered membrane fusion and fission events. While initially determined to share many of the features of members of the synaptotagmin family of calcium sensors, ferlins in more recent studies have been found to interact directly with non...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00928
更新日期：2017-12-12 00:00:00
abstract：:The continued rise of antibiotic-resistant infections coupled with the limited pipeline of new antimicrobials highlights the pressing need for the development of new antibacterial agents. One potential pathway for new agents is de novo purine biosynthesis as studies have shown that bacteria and lower eukaryotes synthe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00939
更新日期：2019-04-30 00:00:00
abstract：:A soluble and fully functional 10.5 kDa fragment of the 18.2 kDa membrane-bound cytochrome c(552) from Paracoccus denitrificans has been heterologously expressed and (13)C/(15)N-labeled to study the structural features of this protein in both redox states. Well-resolved solution structures of both the reduced and oxid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010615o
更新日期：2001-10-16 00:00:00
abstract：:Tat, an essential human immunodeficiency virus type 1 protein interacts with the transactivation response element (TAR) and stimulates transcription from the viral long-terminal repeat (LTR). Blockage of Tat-TAR interaction halts viral transcription and hence replication. We have found that polyamide nucleic acid (PNA...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000708q
更新日期：2000-09-26 00:00:00
abstract：:Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00514a019
更新日期：1981-05-26 00:00:00
abstract：:BtuB is a bacterial outer-membrane protein that transports vitamin B(12). Spectroscopic studies using site-directed spin labeling (SDSL) indicate that the N-terminus of BtuB undergoes a dramatic structural change from a docked (folded) to an undocked (unfolded) configuration upon substrate binding. However, this drama...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035439t
更新日期：2003-11-18 00:00:00
abstract：:We have recently reported the cation-induced self-assembly of DNA oligomers of the general sequence C4T4G4T1-4G4 into high-molecular weight multistranded structures [Marotta, S.P., Tamburri, P.A., and Sheardy, R.D. (1996) Biochemistry 35, 10484-10492]. The architecture of the proposed structure consists of a series of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973009v
更新日期：1998-03-03 00:00:00
abstract：:Crk-II is a signaling adaptor protein that is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an Src homology 2 domain (SH2) followed by two Src homology 3 (SH3) domains. The structures and ligand-binding properties of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060590z
更新日期：2006-07-25 00:00:00
abstract：:We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in cl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00251a044
更新日期：1994-11-29 00:00:00
abstract：:Deamidation of proteins is one of the most prevalent post-translational modifications found upon aging, and in age-onset diseases. Specific asparagine and glutamine residues are often selectively deamidated during this process. In the human lens, deamidation has been shown to occur in many crystallins, but it is not c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00185
更新日期：2015-08-11 00:00:00
abstract：:Halogen bonding is a weak chemical force that has so far mostly found applications in crystal engineering. Despite its potential for use in drug discovery, as a new molecular tool in the direction of molecular recognition events, it has rarely been assessed in biopolymers. Motivated by this fact, we have developed a p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00429
更新日期：2017-06-27 00:00:00
