Competitive binding of ATP and the fluorescent substrate analogue 2',3'-O-(2,4,6-trinitrophenylcyclohexadienylidine) adenosine 5'-triphosphate to the gastric H+,K+-ATPase: evidence for two classes of nucleotide sites.

abstract：:Plexins are transmembrane proteins that serve as guidance receptors during angiogenesis, lymphangiogenesis, neuronal development, and zebrafish fin regeneration, with a putative role in cancer metastasis. Receptor dimerization or clustering, induced by extracellular ligand binding but modulated in part by the plexin t...

journal_title：Biochemistry

authors： Barton R,Khakbaz P,Bera I,Klauda JB,Iovine MK,Berger BW

更新日期：2016-09-06 00:00:00

abstract：:A new procedure to cleave tryptophanyl peptide bonds in high yield is reported. The method involves treatment of the S-alkylated protein with o-iodosobenzoic acid. The procedure is highly selective for tryptophan and does not modify tyrosine or histidine, but may convert methionine to its sulfoxide derivative. The yie...

journal_title：Biochemistry

authors： Mahoney WC,Hermodson MA

更新日期：1979-08-21 00:00:00

abstract：:Studies of intragenomic homologues in bacterial genomes can provide valuable insights into functional divergence. Three GTP cyclohydrolase II homologues in the Streptomyces coelicolor genome have been shown to catalyze two related but distinct reactions [Spoonamore, J. E., Dahlgran, A. L., Jacobsen, N. E., and Bandari...

journal_title：Biochemistry

authors： Spoonamore JE,Bandarian V

更新日期：2008-02-26 00:00:00

abstract：:On the basis of the available high-resolution structures of mandelate racemase (MR) from Pseudomonas putida [Landro, J. A., Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, J., & Petsko, G. A. (1994) Biochemistry 33, 635-643], Lys 166 and His 297 are positioned appropriat...

journal_title：Biochemistry

authors： Kallarakal AT,Mitra B,Kozarich JW,Gerlt JA,Clifton JG,Petsko GA,Kenyon GL

更新日期：1995-03-07 00:00:00

abstract：:The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...

journal_title：Biochemistry

authors： Takahashi N,Ishii I,Ishihara H,Mori M,Tejima S,Jefferis R,Endo S,Arata Y

更新日期：1987-02-24 00:00:00

abstract：:The motion of the ethanolamine head group in unsonicated lipid bilayers above and below the phase transition is studied by means of deuterium and phosphorus magnetic resonance. For this purpose, dipalmitoyl-3-sn-phosphatidylethanolamine is selectively deuterated at the two ethanolamine carbon atoms. The deuterium quad...

journal_title：Biochemistry

authors： Seelig J,Gally H

更新日期：1976-11-30 00:00:00

abstract：:The contact between EcoRI endonuclease and the "primary clamp" phosphate of its recognition site pGAATTC is absolutely required for recognition of the canonical and all variant DNA sites. We have probed this contact using oligonucleotides containing single stereospecific (Rp)- or (Sp)- phosphorothioates (Ps). At the G...

journal_title：Biochemistry

authors： Kurpiewski MR,Koziolkiewicz M,Wilk A,Stec WJ,Jen-Jacobson L

更新日期：1996-07-09 00:00:00

abstract：:Isotope effects have been measured with Mg2+ as the activator and L-malate labeled with deuterium or tritium at carbon 2 as the substrate over the pH range 4-10. Comparison of the nearly pH-independent deuterium-isotope effect on V/Kmalate of 1.5 with the tritium effect of 2.0 by the method of Northrop (Northrop, D.B....

journal_title：Biochemistry

authors： Schimerlik MI,Grimshaw CE,Cleland WW

更新日期：1977-02-22 00:00:00

abstract：:The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...

journal_title：Biochemistry

authors： Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

更新日期：2008-02-19 00:00:00

abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...

journal_title：Biochemistry

authors： Wolf B,Hanlon S

更新日期：1975-04-22 00:00:00

abstract：:Translocation of DNA during in vitro DNA synthesis on nuclear matrix bound replicational assemblies from regenerating rat liver was determined by measuring the processivity (average number of nucleotides added following one productive binding event of the polymerase to the DNA template) of nuclear matrix bound DNA pol...

journal_title：Biochemistry

authors： Tubo RA,Martelli AM,Berezney R

更新日期：1987-09-08 00:00:00

abstract：:In spite of a nuclear targeting sequence, a substantial amount of dual-specificity tyrosine phosphorylation-regulated kinase (Dyrk1A) is located within the cytoplasm of neurons. Analysis of fractionated rat brains revealed that the majority of Dyrk1A was in the postnuclear precipitate. The kinase in this fraction was ...

journal_title：Biochemistry

authors： Murakami N,Bolton D,Hwang YW

更新日期：2009-10-06 00:00:00

abstract：:In order to define structural regions in thrombin that interact with hirudin, the N alpha-dinitrofluorobenzyl analogue of an undecapeptide was synthesized corresponding to residues 54-64 of hirudin [GDFEEIPEEY(O35SO3)L (DNFB-[35S]Hir54-64)]. DNFB-[35S]Hir54-64 was reacted at a 10-fold molar excess with human alpha-thr...

journal_title：Biochemistry

authors： Bourdon P,Fenton JW 2nd,Maraganore JM

更新日期：1990-07-10 00:00:00

abstract：:Cdc31, the Saccharomyces cerevisiae centrin, is an EF-hand calcium-binding protein essential for the cell division and mRNA nuclear export. We used biophysical techniques to investigate its calcium, magnesium, and protein target binding properties as well as their conformations in solution. We show here that Cdc31 dis...

journal_title：Biochemistry

authors： Miron S,Durand D,Chilom C,Pérez J,Craescu CT

更新日期：2011-07-26 00:00:00

abstract：:A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3 mi...

journal_title：Biochemistry

authors： Kusumi A,Tsuji A,Murata M,Sako Y,Yoshizawa AC,Kagiwada S,Hayakawa T,Ohnishi S

更新日期：1991-07-02 00:00:00

abstract：:A method is presented for the rapid determination of substrate selection in a manner that is not restricted to conditions of metabolic and isotopic steady state. Competition between several substrates can be assessed directly and continuously in a single experiment, allowing the effect of interventions to be studied. ...

journal_title：Biochemistry

authors： Malloy CR,Thompson JR,Jeffrey FM,Sherry AD

更新日期：1990-07-24 00:00:00

abstract：:MantATP [2'(3')-O-(-N-methylanthraniloyl)-adenosine 5'-triphosphate] was employed as a fluorescence probe of the nucleotide-binding sites of dynein from sea urchin sperm flagella. MantATP binds specifically with enhanced fluorescence (approximately 2.2-fold), homogeneous lifetime (8.4 ns), and high anisotropy (r appro...

journal_title：Biochemistry

authors： Mocz G,Helms MK,Jameson DM,Gibbons IR

更新日期：1998-07-07 00:00:00

abstract：:The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as leaving groups is described. It is demonstrated that th...

journal_title：Biochemistry

authors： Stennicke HR,Mortensen UH,Breddam K

更新日期：1996-06-04 00:00:00

abstract：:Human hemoglobin produced in the Escherichia coli coexpression system of Hernan et al. [(1992) Biochemistry 31, 8619-8628] has been transformed into a functionally homogeneous protein whose properties closely approximate those of normal hemoglobin A. Both of the alpha and beta chains of this hemoglobin contain a valin...

journal_title：Biochemistry

authors： Hui HL,Kavanaugh JS,Doyle ML,Wierzba A,Rogers PH,Arnone A,Holt JM,Ackers GK,Noble RW

更新日期：1999-01-19 00:00:00

abstract：:In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...

journal_title：Biochemistry

authors： Dunford HB,Hewson WD

更新日期：1977-06-28 00:00:00

abstract：:Secretory carrier membrane protein 2 (SCAMP2) functions in late steps of membrane fusion in calcium-dependent granule exocytosis. A basic/hydrophobic peptide segment within SCAMP2 (SCAMP2 E: CWYRPIYKAFR) has been implicated in this function and shown to bind and sequester phosphatidylinositol 4,5-bisphosphate [PI(4,5)...

journal_title：Biochemistry

authors： Liao H,Ellena J,Liu L,Szabo G,Cafiso D,Castle D

更新日期：2007-09-25 00:00:00

abstract：:The photolyase/cryptochrome family is a large family of flavoproteins that encompasses DNA repair proteins, photolyases, and cryptochromes that regulate blue-light-dependent growth and development in plants, and light-dependent and light-independent circadian clock setting in animals. Phylogenetic analysis has reveale...

journal_title：Biochemistry

authors： Oztürk N,Kao YT,Selby CP,Kavakli IH,Partch CL,Zhong D,Sancar A

更新日期：2008-09-30 00:00:00

abstract：:The thermodynamics and kinetics of magnesium binding to tRNA(Phe)(yeast) have been studied directly by 25Mg NMR. In 0.17 M Na+(aq), tRNA(Phe) exists in its native conformation and the number of strong binding sites (Ka greater than or equal to 10(4)) was estimated to be 3-4 by titration experiments, in agreement with ...

journal_title：Biochemistry

authors： Reid SS,Cowan JA

更新日期：1990-06-26 00:00:00

abstract：:The toll that Streptococcus pneumoniae exacts on the welfare of humanity is enormous. This organism claims the lives of approximately 3700 people daily, the majority of whom are children below the age of 5, and the situation could worsen due to the increasing incidence of pernicious, multiple-antibiotic-resistant stra...

journal_title：Biochemistry

authors： Andreassi JL 2nd,Dabovic K,Leyh TS

更新日期：2004-12-28 00:00:00

abstract：:The topography of the photosynthetic reaction center (RC) polypeptides (H, M, and L) was investigated by proteolysis and radioiodination of membrane vesicles isolated from Rhodopseudomonas sphaeroides. Chromatophores, obtained from French-pressed cell lysates, are closed vesicles' and oriented inside out with respect ...

journal_title：Biochemistry

authors： Bachmann RC,Gillies K,Takemoto JY

更新日期：1981-08-04 00:00:00

abstract：:Sf9 cells infected with a recombinant baculovirus containing the gene for human prorenin were cultured in the presence of [3H]mannose. In vivo labeled prorenin was isolated by immunoprecipitation from the culture medium and digested with Pronase. The oligosaccharide structures on the resulting glycopeptides were analy...

journal_title：Biochemistry

authors： Aeed PA,Elhammer AP

更新日期：1994-07-26 00:00:00

abstract：:AMSH, a deubiquitinating enzyme (DUB) with exquisite specificity for Lys63-linked polyubiquitin chains, is an endosome-associated DUB that regulates sorting of activated cell-surface signaling receptors to the lysosome, a process mediated by the members of the endosomal sorting complexes required for transport (ESCRT)...

journal_title：Biochemistry

authors： Davies CW,Paul LN,Das C

更新日期：2013-11-05 00:00:00

abstract：:Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...

journal_title：Biochemistry

authors： Oller AR,Vanden Broek W,Conrad M,Topal MD

更新日期：1991-03-05 00:00:00

abstract：:The oxygen transfer to p-hydroxybenzoate catalyzed by p-hydroxybenzoate hydroxylase (PHBH) has been shown to occur via a C4a-hydroperoxide of the flavin. Two factors are likely to be important in facilitating the transfer of oxygen from the C4a-hydroperoxide to the substrate. (a) The positive electrostatic potential o...

journal_title：Biochemistry

authors： Ortiz-Maldonado M,Aeschliman SM,Ballou DP,Massey V

更新日期：2001-07-31 00:00:00

abstract：:The salivary antimicrobial peptide histatin-5 is able to aggregate and fuse negatively charged small unilamellar vesicles, and this fusogenic activity is selectively induced by the presence of zinc ions. Circular dichroism spectroscopy shows that histatin-5, in the presence of negatively charged vesicles and zinc ions...

journal_title：Biochemistry

authors： Melino S,Rufini S,Sette M,Morero R,Grottesi A,Paci M,Petruzzelli R

更新日期：1999-07-27 00:00:00