当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Two homologues encoding human UDP-glucose:glycoprotein glucosyltransferase differ in mRNA expression and enzymatic activity.

Two homologues encoding human UDP-glucose:glycoprotein glucosyltransferase differ in mRNA expression and enzymatic activity.

Abstract：

:UDP-glucose:glycoprotein glucosyltransferase (UGT) is a soluble protein of the endoplasmic reticulum (ER) that operates as a gatekeeper for quality control by preventing transport of improperly folded glycoproteins out of the ER. We report the isolation of two cDNAs encoding human UDP-glucose:glycoprotein glucosyltransferase homologues. HUGT1 encodes a 1555 amino acid polypeptide that, upon cleavage of an N-terminal signal peptide, is predicted to produce a soluble 173 kDa protein with the ER retrieval signal REEL. HUGT2 encodes a 1516 amino acid polypeptide that also contains a signal peptide and the ER retrieval signal HDEL. HUGT1 shares 55% identity with HUGT2 and 31-45% identity with Drosophila, Caenorhabditis elegans, and Schizosaccharomyces pombe homologues, with most extensive conservation of residues in the carboxy-terminal fifth of the protein, the proposed catalytic domain. HUGT1 is expressed as multiple mRNA species that are induced to similar extents upon disruption of protein folding in the ER. In contrast, HUGT2 is transcribed as a single mRNA species that is not induced under similar conditions. HUGT1 and HUGT2 mRNAs are broadly expressed in multiple tissues and differ slightly in their tissue distribution. The HUGT1 and HUGT2 cDNAs were expressed by transient transfection in COS-1 monkey cells to obtain similar levels of protein localized to the ER. Extracts from HUGT1-transfected cells displayed a 27-fold increase in the transfer of [(14)C]glucose from UDP-[(14)C]glucose to denatured substrates. Despite its high degree of sequence identity with HUGT1, the expressed recombinant HUGT2 protein was not functional under the conditions optimized for HUGT1. Site-directed alanine mutagenesis within a highly conserved region of HUGT1 identified four residues that are essential for catalytic function.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Arnold SM,Fessler LI,Fessler JH,Kaufman RJ

doi

10.1021/bi9916473

subject

Has Abstract

pub_date

2000-03-07 00:00:00

pages

2149-63

issue

9

eissn

0006-2960

issn

1520-4995

pii

bi9916473

journal_volume

39

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Resonance Raman and electronic absorption spectral studies of some beta-(2-furyl)acryloylglyceraldehyde-3-phosphate dehydrogenases.

    abstract：:Electronic absorption and resonance Raman spectra, together with rates of arsenolysis, are used to show that the acylated subunits of the rabbit furylacryloylglyceraldehyde-3-phosphate dehydrogenase (FA-rabbit enzyme), but not the FA-sturgeon enzyme, exist as a mixed population of at least two forms. One form of the F...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00515a023

    authors： Storer AC,Phelps DJ,Carey PR

    更新日期：1981-06-09 00:00:00

  • Kinetics of peptide binding to the class II MHC protein I-Ek.

    abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9921337

    authors： Kasson PM,Rabinowitz JD,Schmitt L,Davis MM,McConnell HM

    更新日期：2000-02-08 00:00:00

  • Electron/proton coupling in bacterial nitric oxide reductase during reduction of oxygen.

    abstract：:The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050524h

    authors： Flock U,Watmough NJ,Adelroth P

    更新日期：2005-08-09 00:00:00

  • Ion effects on the lac repressor--operator equilibrium.

    abstract：:The effects of ions on the interaction of lac repressor protein and operator DNA have been studied by the membrane filter technique. The equilibrium association constant was determined as a function of monovalent and divalent cation concentrations, anions, and pH. The binding of repressor and operator is extremely sen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00516a027

    authors： Barkley MD,Lewis PA,Sullivan GE

    更新日期：1981-06-23 00:00:00

  • A method for assessing the stability of a membrane protein.

    abstract：:The integral membrane protein diacylglycerol kinase (DGK) from Escherichia coli has been reversibly unfolded in a protein/detergent/mixed micelle system by varying the molar ratio of n-decyl beta-D-maltoside (DM) and sodium dodecyl sulfate (SDS). Unfolding was monitored by circular dichroism (CD) and ultraviolet (UV) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi963095j

    authors： Lau FW,Bowie JU

    更新日期：1997-05-13 00:00:00

  • The SIV tilted peptide induces cylindrical reverse micelles in supported lipid bilayers.

    abstract：:Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negativ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060317x

    authors： El Kirat K,Dufrêne YF,Lins L,Brasseur R

    更新日期：2006-08-01 00:00:00

  • The observed inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate for HIV-1 reverse transcriptase depends on the length of the poly(rA) region of the template.

    abstract：:The inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) against HIV-1 reverse transcriptase (HIV-1 RT) has been further evaluated. The results indicate that the previously reported low Ki values for AZTTP against HIV-1 RT (2.35 nM) are due neither to the to the direct tight binding of AZTTP to HIV...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00120a013

    authors： Ma QF,Bathurst IC,Barr PJ,Kenyon GL

    更新日期：1992-02-11 00:00:00

  • Crystal structure of substrate-free Pseudomonas putida cytochrome P-450.

    abstract：:The crystal structure of Pseudomonas putida cytochrome P-450cam in the substrate-free form has been refined at 2.20-A resolution and compared to the substrate-bound form of the enzyme. In the absence of the substrate camphor, the P-450cam heme iron atom is hexacoordinate with the sulfur atom of Cys-357 providing one a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00366a049

    authors： Poulos TL,Finzel BC,Howard AJ

    更新日期：1986-09-09 00:00:00

  • Redundancy of mammalian proteasome beta subunit function during endoplasmic reticulum associated degradation.

    abstract：:Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectivel...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011322y

    authors： Oberdorf J,Carlson EJ,Skach WR

    更新日期：2001-11-06 00:00:00

  • The role of Gly-4 of human cystatin A (stefin A) in the binding of target proteinases. Characterization by kinetic and equilibrium methods of the interactions of cystatin A Gly-4 mutants with papain, cathepsin B, and cathepsin L.

    abstract：:The importance of the evolutionarily conserved Gly-4 residue for the affinity and kinetics of interaction of cystatin A with several cysteine proteinases was assessed by site-directed mutagenesis. Even the smallest replacement, by Ala, resulted in approximately 1000-, approximately 10- and approximately 6000-fold decr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980026r

    authors： Estrada S,Nycander M,Hill NJ,Craven CJ,Waltho JP,Björk I

    更新日期：1998-05-19 00:00:00

  • Site-directed mutagenesis and 1H NMR spectroscopy of an interdomain segment in the pyruvate dehydrogenase multienzyme complex of Escherichia coli.

    abstract：:Deletion of two of the three homologous lipoyl domains that form the N-terminal half of each dihydrolipoamide acetyltransferase (E2p) polypeptide chain of the Escherichia coli pyruvate dehydrogenase complex can be achieved by in vitro deletion in the structural gene aceF. A site-directed mutagenesis of this shortened ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00401a044

    authors： Texter FL,Radford SE,Laue ED,Perham RN,Miles JS,Guest JR

    更新日期：1988-01-12 00:00:00

  • Picosecond resolution of tyrosine fluorescence and anisotropy decays by 2-GHz frequency-domain fluorometry.

    abstract：:We extended the technique of frequency-domain fluorometry to an upper frequency limit of 2000 MHz. This was accomplished by using the harmonic content of a laser pulse train (3.76 MHz, 5 ps) from a synchronously pumped and cavity-dumped dye laser. We used a microchannel plate photomultiplier as the detector to obtain ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00375a012

    authors： Lakowicz JR,Laczko G,Gryczynski I

    更新日期：1987-01-13 00:00:00

  • A monoclonal antibody against brain calmodulin-dependent protein kinase type II detects putative conformational changes induced by Ca2+-calmodulin.

    abstract：:A mouse monoclonal IgG1 antibody has been generated against the soluble form of the calmodulin-dependent protein kinase type II. This antibody recognizes both the soluble and cytoskeletal forms of the enzyme, requiring Ca2+ (EC50 = 20 microM) for the interaction. Other divalent cations such as Zn2+, Mn2+, Cd2+, Co2+, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00417a060

    authors： LeVine H 3rd,Su JL,Sahyoun NE

    更新日期：1988-08-23 00:00:00

  • Equilibrium binding of [3H]tubocurarine and [3H]acetylcholine by Torpedo postsynaptic membranes: stoichiometry and ligand interactions.

    abstract：:Studies are presented of the equilibrium binding of [3H]-d-tubocurarine (dTC) and [3H]acetylcholine (AcCh) to Torpedo postsynaptic membranes. The saturable binding of [3H]dTC is characterized by two affinities: Kd1 = 33 +/- 6 nM and Kd2 = 7.7 +/- 4.6 microM, with equal numbers of binding sites. Both components are com...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00591a032

    authors： Neubig RR,Cohen JB

    更新日期：1979-11-27 00:00:00

  • Aluminum fluoride activation of bovine transducin induces two distinct conformational changes in the alpha subunit.

    abstract：:We have used resonance energy transfer to read out the interactions of the alpha subunit of transducin (alpha T) with the transducin beta gamma subunit complex (beta gamma T) and to compare the rate of aluminum fluoride-induced alpha T activation, as reflected by the enhancement of the alpha T tryptophan fluorescence,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00199a046

    authors： Mittal R,Cerione RA,Erickson JW

    更新日期：1994-08-23 00:00:00

  • Crystal Structures of Apo and Liganded 4-Oxalocrotonate Decarboxylase Uncover a Structural Basis for the Metal-Assisted Decarboxylation of a Vinylogous β-Keto Acid.

    abstract：:The enzymes in the catechol meta-fission pathway have been studied for more than 50 years in several species of bacteria capable of degrading a number of aromatic compounds. In a related pathway, naphthalene, a toxic polycyclic aromatic hydrocarbon, is fully degraded to intermediates of the tricarboxylic acid cycle by...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00050

    authors： Guimarães SL,Coitinho JB,Costa DM,Araújo SS,Whitman CP,Nagem RA

    更新日期：2016-05-10 00:00:00

  • X-ray structure of the magnesium(II).ADP.vanadate complex of the Dictyostelium discoideum myosin motor domain to 1.9 A resolution.

    abstract：:The structure of the vanadate-trapped ADP complex of a truncated head of Dictyostelium myosin II consisting of residues Asp 2-Asn 762 has been determined by molecular replacement at 1.9 A resolution and refined to a crystallographic R-factor of 19.4%. The crystals belong to the orthorhombic space group C2221 where a =...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952633+

    authors： Smith CA,Rayment I

    更新日期：1996-04-30 00:00:00

  • Substrate activation by acyl-CoA dehydrogenases: transition-state stabilization and pKs of involved functional groups.

    abstract：:The mechanism by which acyl-CoA dehydrogenases initiate catalysis was studied by using p-substituted phenylacetyl-CoAs (substituents-NO2, -CN, and CH3CO-), 3S-C8-, and 3'-dephospho-3S-C8CoA. These analogues lack a beta C-H and cannot undergo alpha,beta-dehydrogenation. Instead they deprotonate at alpha C-H at pH > or ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971827h

    authors： Vock P,Engst S,Eder M,Ghisla S

    更新日期：1998-02-17 00:00:00

  • DNA deformability as a recognition feature in the reverb response element.

    abstract：:Most nuclear receptors recognize the same consensus hexameric sequence, AGGTCA. An important question has been how the various members of this transcription factor family distinguish identity features in these closely related DNA sites. We determined structures from several crystal forms of the RevErb-DNA complex and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011086r

    authors： Sierk ML,Zhao Q,Rastinejad F

    更新日期：2001-10-30 00:00:00

  • Substrate binding to the peripheral site of acetylcholinesterase initiates enzymatic catalysis. Substrate inhibition arises as a secondary effect.

    abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9813577

    authors： Szegletes T,Mallender WD,Thomas PJ,Rosenberry TL

    更新日期：1999-01-05 00:00:00

  • Spectroscopic Investigation of Cysteamine Dioxygenase.

    abstract：:Thiol dioxygenases are mononuclear non-heme FeII-dependent metalloenzymes that initiate the oxidative catabolism of thiol-containing substrates to their respective sulfinates. Cysteine dioxygenase (CDO), the best characterized mammalian thiol dioxygenase, contains a three-histidine (3-His) coordination environment rat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00267

    authors： Fernandez RL,Dillon SL,Stipanuk MH,Fox BG,Brunold TC

    更新日期：2020-07-07 00:00:00

  • Mechanism of inhibition of the class C beta-lactamase of Enterobacter cloacae P99 by phosphonate monoesters.

    abstract：:The class C serine beta-lactamase of Enterobacter cloacae P99 was inhibited by a series of aryl methylphosphonate monoester monoanions. The effectiveness of these inhibitors was promoted by an acylamido substituent on the methyl group and a good leaving group at phosphorus. The former preference suggests that noncoval...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00140a024

    authors： Rahil J,Pratt RF

    更新日期：1992-06-30 00:00:00

  • Acetyltransfer precedes uridylyltransfer in the formation of UDP-N-acetylglucosamine in separable active sites of the bifunctional GlmU protein of Escherichia coli.

    abstract：:The GlmU protein is a bifunctional enzyme with both acetyltransferase and uridylyltransferase (pyrophosphorylase) activities which catalyzes the transformation of glucosamine-1-P, UTP, and acetyl-CoA to UDP-N-acetylglucosamine [Mengin-Lecreulx, D., & van Heijenoort, J. (1994) J. Bacteriol. 176, 5788-5795], a fundament...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952275a

    authors： Gehring AM,Lees WJ,Mindiola DJ,Walsh CT,Brown ED

    更新日期：1996-01-16 00:00:00

  • Immunophilin AtFKBP13 sustains all peptidyl-prolyl isomerase activity in the thylakoid lumen from Arabidopsis thaliana deficient in AtCYP20-2.

    abstract：:The physiological roles of immunophilins are unclear, but many possess peptidyl-prolyl isomerase (PPIase) activity, and they have been found in all organisms examined to date, implying that they are involved in fundamental, protein-folding processes. The chloroplast thylakoid lumen of the higher plant Arabidopsis thal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700426q

    authors： Edvardsson A,Shapiguzov A,Petersson UA,Schröder WP,Vener AV

    更新日期：2007-08-21 00:00:00

  • Structural studies of the contractile tail sheath protein of bacteriophage T4. 2. Structural analyses of the tail sheath protein, Gp18, by limited proteolysis, immunoblotting, and immunoelectron microscopy.

    abstract：:The molecular structure of the T4 phage tail sheath protein, gp18, was studied by limited proteolysis, immunoblotting, and immunoelectron microscopy. Gp18 is extremely resistant to proteolysis in the assembled form of either extended or contracted sheaths, but it is readily cleaved by proteases in the monomeric form, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00473a009

    authors： Arisaka F,Takeda S,Funane K,Nishijima N,Ishii S

    更新日期：1990-05-29 00:00:00

  • Characterization of unique DNA-binding and transcriptional-activation functions in the carboxyl-terminal extension of the zinc finger region in the human vitamin D receptor.

    abstract：:The vitamin D receptor (VDR) binds 1,25-dihydroxyvitamin D(3) and mediates its actions on gene transcription by heterodimerizing with retinoid X receptors (RXRs) on direct repeat (DR+3) vitamin D responsive elements (VDREs) located in target genes. The VDRE binding function of VDR has been primarily ascribed to the zi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9916574

    authors： Hsieh JC,Whitfield GK,Oza AK,Dang HT,Price JN,Galligan MA,Jurutka PW,Thompson PD,Haussler CA,Haussler MR

    更新日期：1999-12-07 00:00:00

  • Effects of guanidine hydrochloride on the proton inventory of proteins: implications on interpretations of protein stability.

    abstract：:The DeltaG degrees (N)(-)(D) value obtained from extrapolation to zero denaturant concentration by the linear extrapolation method (LEM) is commonly interpreted to represent the Gibbs energy difference between native (N) and denatured (D) ensembles at the limit of zero denaturant concentration. For DeltaG degrees (N)(...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001071d

    authors： Bolen DW,Yang M

    更新日期：2000-12-12 00:00:00

  • Protonation state of Glu142 differs in the green- and blue-absorbing variants of proteorhodopsin.

    abstract：:Proteorhodopsins are a recently discovered class of microbial rhodopsins, ubiquitous in marine bacteria. Over 4000 variants have thus far been discovered, distributed throughout the oceans of the world. Most variants fall into one of two major groups, green- or blue-absorbing proteorhodopsin (GPR and BPR, respectively...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7018964

    authors： Kralj JM,Bergo VB,Amsden JJ,Spudich EN,Spudich JL,Rothschild KJ

    更新日期：2008-03-18 00:00:00

  • Direct determination of the association constant between elongation factor Tu X GTP and aminoacyl-tRNA using fluorescence.

    abstract：:We have investigated the formation of the aa-tRNA X EF-Tu X GTP ternary complex spectroscopically by monitoring a fluorescence change that accompanies the association of EF-Tu X GTP with Phe-tRNAPhe-F8, a functionally active analogue of Phe-tRNAPhe with a fluorescein moiety covalently attached to the s4U-8 base. With ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00324a023

    authors： Abrahamson JK,Laue TM,Miller DL,Johnson AE

    更新日期：1985-01-29 00:00:00

  • Membrane insertion and dissociation processes of a model transmembrane helix.

    abstract：:An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026191v

    authors： Yano Y,Matsuzaki K

    更新日期：2002-10-15 00:00:00