Abstract:
:Incorporation of nonproteinogenic amino acids in small polypeptides synthesized by nonribosomal peptide synthetases (NRPS) significantly contributes to their biological activity. In these peptides, conversion of L-amino acids to the corresponding D-isomer is catalyzed by specialized NRPS modules that utilize an epimerization (E) domain. To understand the basis for the specific interaction of E domains with PCP domains (peptidyl carrier proteins, also described as T domains) and to investigate their substrate tolerance, we constructed a set of eight fusion proteins. The gene fragments encoding E and PCP-E domains of TycA (A-PCP-E), the one module tyrocidine synthetase A, were fused to different gene fragments encoding A and A-PCP domains, resulting in A/PCP-E and A-PCP/E types of fusion proteins (slash indicates site of fusion). We were able to show that the E domain of TycA, usually epimerizing Phe, does also accept the alternate substrates Trp, Ile, and Val, although with reduced efficiency. Interestingly, however, an epimerization activity was only observed in the case of fusion proteins where the PCP domain originates from modules containing an E domain. Sequence comparison revealed that such PCPs possess significant differences in the signature Ppant binding motif (CoreT: [GGDSI]), when compared to those carrier proteins, originating from ordinary C-A-PCP elongation modules (CoreT: [GGHSL]). By means of mutational analysis, we could show that epimerization activity is influenced by the nature of amino acid residues in proximity to the cofactor Ppant binding site. The aspartate residue in front of the invariant serine (Ppant binding site) especially seems to play an important role for the proper interaction between PCP and the E domain, as well as the presentation of the aminoacyl-S-Ppant substrate in the course of substrate epimerization. In conclusion, specialized PCP domains are needed for a productive interaction with E domains when constructing hybrid enzymes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Linne U,Doekel S,Marahiel MAdoi
10.1021/bi011595tsubject
Has Abstractpub_date
2001-12-25 00:00:00pages
15824-34issue
51eissn
0006-2960issn
1520-4995pii
bi011595tjournal_volume
40pub_type
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