Abstract：
:The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second does not. Dissociation constants were as follows: K(11Lf) = 1.5 ± 0.2 μM, and K(12Lf) = 11 ± 2 μM. Two slightly different interactions of T or TFe(2) with Cp are detected for the first time. They are both independent of pH and μ and occur with 1:1 stoichiometry: K(1T) = 19 ± 7 μM, and K(1TFe2) = 12 ± 4 μM. These results can improve our understanding of the probable process of the transfer of iron from Cp to T in iron and copper transport and homeostasis.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Ha-Duong NT,Eid C,Hémadi M,El Hage Chahine JMdoi
10.1021/bi1014503subject
Has Abstractpub_date
2010-12-07 00:00:00pages
10261-3issue
48eissn
0006-2960issn
1520-4995journal_volume
49pub_type
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