Abstract:
:The nitrite anion is known to oxidize and degrade hemoglobin (Hb). Recent literature reports suggest a nitrite reductase activity for Hb, converting nitrite into nitric oxide. Surprisingly, no structural information about Hb-nitrite interactions has been reported. We have determined the crystal structure of the ferric Hb-nitrite complex at 1.80 A resolution. The nitrite ligand adopts the uncommon O-nitrito binding mode. In addition, the nitrito conformations in the alpha and beta subunits are different, reflecting subtle effects of the distal His in orienting the nitrite ligand in the O-nitrito binding mode.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Yi J,Safo MK,Richter-Addo GBdoi
10.1021/bi801015csubject
Has Abstractpub_date
2008-08-12 00:00:00pages
8247-9issue
32eissn
0006-2960issn
1520-4995journal_volume
47pub_type
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